BrabA.19355.a

Type IV secreted protein VceC; flagellar biosynthetic protein FliO

CENTER ID: BrabA.19355.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.19355.a.B2.GE40903 soluble domain 37 418
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.11.peg.1769
UniProt: Q2YQ34

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKEWLSGIIG ESAANIVGFV LIFAIILGGI FVVLGIIRRF SGGTFVSSGR MRQPRLSVMD AAAVDSRRKL VLIRRDDVEH LLLIGGPTDV VVEQNIVLES RAGTHAPGTH ALGTHAQSSR IEPEHIERFR QHEVLANEKT ERPALSVQSQ PGDEREETIQ LSSAIAAPVY RKPEPPKAEN VRPAPSEPAP MRAPQPPVTP PPAAPRPEAP ASQVSEPHPR PASNYPSQPR TVLPPRPAPS AAPNSVRAHP AYPLSQVSRG VVSSTSNIQA ATVASAATAN LGSIVPERES AAPAKPAAPA PNQPERSERP ERPERPQQRI EPVVPFVAQE VRQPESADDD LDDLGGTLHD AIMADLGSNA GKDENMEELD TASLEDELLS SLDISPTDEQ PEDAKAEETI EDEMEKLLGE LTKGETRN
NT Sequence
atgaaggaat ggctcagcgg catcatcggt gaaagcgcgg ctaatattgt cggctttgtg cttatatttg caatcatcct tggcggaatt tttgttgttc tcggcatcat tcgccgtttc agcggcggca catttgtatc cagcgggcgc atgcgccagc cccgcctttc ggtgatggat gccgccgcgg tagacagccg gcgcaagctg gttctgatcc gccgcgacga tgtggaacat ctgctcctga tcggtggccc gaccgatgtg gttgtcgaac agaatatcgt tctggaatcg cgcgctggca cccatgcccc tggaactcat gcccttggta cccacgcaca gagctcgcgt atcgaaccgg aacatattga acgcttccgc cagcacgaag tgcttgccaa tgagaagacg gaacggcctg ccctttccgt ccagtcccag cccggagacg aacgcgagga aactattcag ctaagctcgg ccatcgcggc gcccgtctat cgcaagccgg agccgccgaa ggctgagaac gtccggcccg cgccctccga acccgcacca atgcgggcac cgcagcctcc cgtgacgcca ccgccagccg cgccgcgccc tgaagcgcct gcgtcgcagg tttccgagcc gcatccgcgc ccagcttcaa attacccgtc acagccgcgc acggttcttc ccccgcgccc cgcgccatcg gctgccccaa acagcgttcg cgcgcatcca gcctatccgc tgagccaggt ttcgcgcggc gtggtaagtt ccacatcaaa tatccaggca gccacggttg catcggccgc gacagcaaat ctgggcagca ttgtgcccga acgcgaatct gccgctccgg caaaacccgc agcgcccgcg ccgaatcagc cggaacgttc agagcgtcca gaacgcccgg aacgcccaca acaacgcatc gagccggttg ttccctttgt cgcccaggaa gtcaggcagc ccgaaagcgc agatgacgat ctggatgacc ttggcggcac attgcatgat gccattatgg ccgatcttgg cagcaatgcc ggtaaagatg aaaatatgga ggagttggac acggcttcgc tggaagacga actactcagc tcgcttgata tttcacctac cgatgaacag ccggaagatg ccaaggccga agaaaccatc gaagacgaaa tggaaaagct gcttggtgag ttgaccaagg gagaaacccg caattag
Details for BrabA.19355.a.B2.GE40903
HARVESTED ON: data unavailable
SEQUENCED ON: 12/27/2016
EXPECTED MW: 42kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMI RRFSGGTFVS SGRMRQPRLS VMDAAAVDSR RKLVLIRRDD VEHLLLIGGP TDVVVEQNIV LESRAGTHAP GTHALGTHAQ SSRIEPEHIE RFRQHEVLAN EKTERPALSV QSQPGDEREE TIQLSSAIAA PVYRKPEPPK AENVRPAPSE PAPMRAPQPP VTPPPAAPRP EAPASQVSEP HPRPASNYPS QPRTVLPPRP APSAAPNSVR AHPAYPLSQV SRGVVSSTSN IQAATVASAA TANLGSIVPE RESAAPAKPA APAPNQPERS ERPERPERPQ QRIEPVVPFV AQEVRQPESA DDDLDDLGGT LHDAIMADLG SNAGKDENME ELDTASLEDE LLSSLDISPT DEQPEDAKAE ETIEDEMEKL LG
Validated NT Sequence
ctcaccaagc agcttttcca tttcgtcttc gatggtttct tcggccttgg catcttccgg ctgttcatcg gtaggtgaaa tatcaagcga gctgagtagt tcgtcttcca gcgaagccgt gtccaactcc tccatatttt catctttacc ggcattgctg ccaagatcgg ccataatggc atcatgcaat gtgccgccaa ggtcatccag atcgtcatct gcgctttcgg gctgcctgac ttcctgggcg acaaagggaa caaccggctc gatgcgttgt tgtgggcgtt ccgggcgttc tggacgctct gaacgttccg gctgattcgg cgcgggcgct gcgggttttg ccggagcggc agattcgcgt tcgggcacaa tgctgcccag atttgctgtc gcggccgatg caaccgtggc tgcctggata tttgatgtgg aacttaccac gccgcgcgaa acctggctca gcggataggc tggatgcgcg cgaacgctgt ttggggcagc cgatggcgcg gggcgcgggg gaagaaccgt gcgcggctgt gacgggtaat ttgaagctgg gcgcggatgc ggctcggaaa cctgcgacgc aggcgcttca gggcgcggcg cggctggcgg tggcgtcacg ggaggctgcg gtgcccgcat tggtgcgggt tcggagggcg cgggccggac gttctcagcc ttcggcggct ccggcttgcg atagacgggc gccgcgatgg ccgagcttag ctgaatagtt tcctcgcgtt cgtctccggg ctgggactgg acggaaaggg caggccgttc cgtcttctca ttggcaagca cttcgtgctg gcggaagcgt tcaatatgtt ccggttcgat acgcgagctc tgtgcgtggg taccaagggc atgagttcca ggggcatggg tgccagcgcg cgattccaga acgatattct gttcgacaac cacatcggtc gggccaccga tcaggagcag atgttccaca tcgtcgcggc ggatcagaac cagcttgcgc cggctgtcta ccgcggcggc atccatcacc gaaaggcggg gctggcgcat gcgcccgctg gatacaaatg tgccgccgct gaaacggcga atcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHIR RFSGGTFVSS GRMRQPRLSV MDAAAVDSRR KLVLIRRDDV EHLLLIGGPT DVVVEQNIVL ESRAGTHAPG THALGTHAQS SRIEPEHIER FRQHEVLANE KTERPALSVQ SQPGDEREET IQLSSAIAAP VYRKPEPPKA ENVRPAPSEP APMRAPQPPV TPPPAAPRPE APASQVSEPH PRPASNYPSQ PRTVLPPRPA PSAAPNSVRA HPAYPLSQVS RGVVSSTSNI QAATVASAAT ANLGSIVPER ESAAPAKPAA PAPNQPERSE RPERPERPQQ RIEPVVPFVA QEVRQPESAD DDLDDLGGTL HDAIMADLGS NAGKDENMEE LDTASLEDEL LSSLDISPTD EQPEDAKAEE TIEDEMEKLL GELTKGETRN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catattcgcc gtttcagcgg cggcacattt gtatccagcg ggcgcatgcg ccagccccgc ctttcggtga tggatgccgc cgcggtagac agccggcgca agctggttct gatccgccgc gacgatgtgg aacatctgct cctgatcggt ggcccgaccg atgtggttgt cgaacagaat atcgttctgg aatcgcgcgc tggcacccat gcccctggaa ctcatgccct tggtacccac gcacagagct cgcgtatcga accggaacat attgaacgct tccgccagca cgaagtgctt gccaatgaga agacggaacg gcctgccctt tccgtccagt cccagcccgg agacgaacgc gaggaaacta ttcagctaag ctcggccatc gcggcgcccg tctatcgcaa gccggagccg ccgaaggctg agaacgtccg gcccgcgccc tccgaacccg caccaatgcg ggcaccgcag cctcccgtga cgccaccgcc agccgcgccg cgccctgaag cgcctgcgtc gcaggtttcc gagccgcatc cgcgcccagc ttcaaattac ccgtcacagc cgcgcacggt tcttcccccg cgccccgcgc catcggctgc cccaaacagc gttcgcgcgc atccagccta tccgctgagc caggtttcgc gcggcgtggt aagttccaca tcaaatatcc aggcagccac ggttgcatcg gccgcgacag caaatctggg cagcattgtg cccgaacgcg aatctgccgc tccggcaaaa cccgcagcgc ccgcgccgaa tcagccggaa cgttcagagc gtccagaacg cccggaacgc ccacaacaac gcatcgagcc ggttgttccc tttgtcgccc aggaagtcag gcagcccgaa agcgcagatg acgatctgga tgaccttggc ggcacattgc atgatgccat tatggccgat cttggcagca atgccggtaa agatgaaaat atggaggagt tggacacggc ttcgctggaa gacgaactac tcagctcgct tgatatttca cctaccgatg aacagccgga agatgccaag gccgaagaaa ccatcgaaga cgaaatggaa aagctgcttg gtgagttgac caagggagaa acccgcaatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat