BrabA.18948.a

Oxidoreductase, N-terminal:Oxidoreductase, C-terminal

CENTER ID: BrabA.18948.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.18948.a.B1.GE38804 full length 1 318
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.18948.a.B1.PS38088 full length 1 318
BrabA.18948.a.B1.PW37688 full length 1 318

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.1676
RefSeq: YP_414979.1
UniProt: Q2YRB8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAPRIAVLGC GYWGGNHIRT LKSLGALQAV SDSNAEKAER FASEFNVPSI PVEELFIHPD IDGIVLALPP QLHAQYAMEA VKNGKDVLVE KPIALDIPVA LAEVEAAREN GRVFMVGHVL RFHPAFEKLL DMVQSGELGD IRYVHSHRVG LGKFHTEFDA LWDFAPHDLS MILAITGEEP NVVRGEGVAI LDHLNDFAHL HMEFPSGIRG HLFASRLNAY RERRLSVTGT KGMAVFDDGE AWERKLALYR HEVWRENDRW AFKSADPVYI QTEEGMPLTR ELQHFMHCIE TRETPRTDGK EAISVLRILT EGTVRHPA
NT Sequence
atggcacctc gtatcgcggt tttgggttgc ggctattggg gcggcaatca cattcgtacc ctgaagagtc tcggcgccct tcaggcagtt tcagactcca acgcggaaaa agcggaacga ttcgcttccg aattcaacgt tcccagcatt ccagtcgagg aactcttcat ccatccggat attgatggca tcgtgctggc gctgccgccg cagcttcatg cgcaatatgc catggaagcg gtgaagaacg gcaaggatgt tctggttgaa aagccgattg cgctcgatat tcccgtagcg cttgccgaag ttgaggccgc gcgcgaaaat ggccgcgtgt tcatggtcgg ccatgtgctc cgcttccatc cggcttttga aaagcttctc gacatggtgc aaagcggcga acttggcgat atccgctatg tgcattcgca ccgcgtcggg cttggcaagt ttcataccga attcgatgcg ctatgggatt tcgcgccgca cgatctttcg atgatccttg ccatcaccgg cgaggagccg aacgtggtgc gcggcgaagg cgttgctatt ctcgatcatc tgaacgattt cgcgcatctt cacatggaat ttccgagcgg aattcgcggc catctttttg cttcgcgtct gaacgcttat cgcgaacgtc gcctcagtgt caccggcacc aagggcatgg ctgttttcga cgatggcgaa gcctgggagc ggaagcttgc cctttacagg catgaggtct ggcgggaaaa cgaccgctgg gctttcaagt ccgccgatcc ggtctatata caaaccgaag aaggtatgcc gctgacgcgc gaattgcagc atttcatgca ttgcatcgaa acgcgtgaaa cgccgcgaac cgacggcaag gaagcgatca gtgtcctgcg cattctcacc gaaggcacgg tgcggcatcc ggcctga
Details for BrabA.18948.a.B1.GE38804
HARVESTED ON: 1/22/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA PRIAVLGCGY WGGNHIRTLK SLGALQAVSD SNAEKAERFA SEFNVPSIPV EELFIHPDID GIVLALPPQL HAQYAMEAVK NGKDVLVEKP IALDIPVALA EVEAARENGR VFMVGHVLRF HPAFEKLLDM VQSGELGDIR YVHSHRVGLG KFHTEFDALW DFAPHDLSMI LAITGEEPNV VRGEGVAILD HLNDFAHLHM EFPSGIRGHL FASRLNAYRE RRLSVTGTKG MAVFDDGEAW ERKLALYRHE VWRENDRWAF KSADPVYIQT EEGMPLTREL QHFMHCIETR ETPRTDGKEA ISVLRILTEG TVRHPA
Validated NT Sequence
atggctcacc accaccacca ccatatggca cctcgtatcg cggttttggg ttgcggctat tggggcggca atcacattcg taccctgaag agtctcggcg cccttcaggc agtttcagac tccaacgcgg aaaaagcgga acgattcgct tccgaattca acgttcccag cattccagtc gaggaactct tcatccatcc ggatattgat ggcatcgtgc tggcgctgcc gccgcagctt catgcgcaat atgccatgga agcggtgaag aacggcaagg atgttctggt tgaaaagccg attgcgcttg atattcccgt agcgcttgcc gaagttgagg ccgcgcgcga aaatggccgc gtgttcatgg tcggccatgt gctccgcttc catccggctt ttgaaaagct tctcgacatg gtgcaaagcg gcgaacttgg cgatatccgc tatgtgcatt cgcaccgcgt cgggcttggc aagtttcata ccgaattcga tgcgctatgg gatttcgcgc cgcacgatct ttcgatgatc cttgccatca ccggcgagga gccgaacgtg gtgcgcggcg aaggcgttgc tattctcgat catctgaacg atttcgcgca tcttcacatg gaatttccga gcggaattcg cggccatctt tttgcttcgc gtctgaacgc ttatcgcgaa cgtcgcctca gtgtcaccgg caccaagggc atggctgttt tcgacgatgg cgaagcctgg gagcggaagc ttgcccttta caggcatgag gtctggcggg aaaacgaccg ctgggctttc aagtccgccg atccggtcta tatacaaacc gaagaaggta tgccgctgac gcgcgaattg cagcatttca tgcattgcat cgaaacgcgt gaaacgccgc gaaccgacgg caaggaagca atcagtgtcc tgcgcattct caccgaaggc acggtgcggc atccggccta agtga
Expected Protein Sequence
MAHHHHHHMA PRIAVLGCGY WGGNHIRTLK SLGALQAVSD SNAEKAERFA SEFNVPSIPV EELFIHPDID GIVLALPPQL HAQYAMEAVK NGKDVLVEKP IALDIPVALA EVEAARENGR VFMVGHVLRF HPAFEKLLDM VQSGELGDIR YVHSHRVGLG KFHTEFDALW DFAPHDLSMI LAITGEEPNV VRGEGVAILD HLNDFAHLHM EFPSGIRGHL FASRLNAYRE RRLSVTGTKG MAVFDDGEAW ERKLALYRHE VWRENDRWAF KSADPVYIQT EEGMPLTREL QHFMHCIETR ETPRTDGKEA ISVLRILTEG TVRHPA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcac ctcgtatcgc ggttttgggt tgcggctatt ggggcggcaa tcacattcgt accctgaaga gtctcggcgc ccttcaggca gtttcagact ccaacgcgga aaaagcggaa cgattcgctt ccgaattcaa cgttcccagc attccagtcg aggaactctt catccatccg gatattgatg gcatcgtgct ggcgctgccg ccgcagcttc atgcgcaata tgccatggaa gcggtgaaga acggcaagga tgttctggtt gaaaagccga ttgcgctcga tattcccgta gcgcttgccg aagttgaggc cgcgcgcgaa aatggccgcg tgttcatggt cggccatgtg ctccgcttcc atccggcttt tgaaaagctt ctcgacatgg tgcaaagcgg cgaacttggc gatatccgct atgtgcattc gcaccgcgtc gggcttggca agtttcatac cgaattcgat gcgctatggg atttcgcgcc gcacgatctt tcgatgatcc ttgccatcac cggcgaggag ccgaacgtgg tgcgcggcga aggcgttgct attctcgatc atctgaacga tttcgcgcat cttcacatgg aatttccgag cggaattcgc ggccatcttt ttgcttcgcg tctgaacgct tatcgcgaac gtcgcctcag tgtcaccggc accaagggca tggctgtttt cgacgatggc gaagcctggg agcggaagct tgccctttac aggcatgagg tctggcggga aaacgaccgc tgggctttca agtccgccga tccggtctat atacaaaccg aagaaggtat gccgctgacg cgcgaattgc agcatttcat gcattgcatc gaaacgcgtg aaacgccgcg aaccgacggc aaggaagcga tcagtgtcct gcgcattctc accgaaggca cggtgcggca tccggcctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BrabA.18948.a.B1.PS38088
PURIFICATION DATe: 10/26/2016
CONCENTRATION: 27.2mg/ml
OBSERVED MW: 35kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 12
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA PRIAVLGCGY WGGNHIRTLK SLGALQAVSD SNAEKAERFA SEFNVPSIPV EELFIHPDID GIVLALPPQL HAQYAMEAVK NGKDVLVEKP IALDIPVALA EVEAARENGR VFMVGHVLRF HPAFEKLLDM VQSGELGDIR YVHSHRVGLG KFHTEFDALW DFAPHDLSMI LAITGEEPNV VRGEGVAILD HLNDFAHLHM EFPSGIRGHL FASRLNAYRE RRLSVTGTKG MAVFDDGEAW ERKLALYRHE VWRENDRWAF KSADPVYIQT EEGMPLTREL QHFMHCIETR ETPRTDGKEA ISVLRILTEG TVRHPA
Validated NT Sequence
atggctcacc accaccacca ccatatggca cctcgtatcg cggttttggg ttgcggctat tggggcggca atcacattcg taccctgaag agtctcggcg cccttcaggc agtttcagac tccaacgcgg aaaaagcgga acgattcgct tccgaattca acgttcccag cattccagtc gaggaactct tcatccatcc ggatattgat ggcatcgtgc tggcgctgcc gccgcagctt catgcgcaat atgccatgga agcggtgaag aacggcaagg atgttctggt tgaaaagccg attgcgcttg atattcccgt agcgcttgcc gaagttgagg ccgcgcgcga aaatggccgc gtgttcatgg tcggccatgt gctccgcttc catccggctt ttgaaaagct tctcgacatg gtgcaaagcg gcgaacttgg cgatatccgc tatgtgcatt cgcaccgcgt cgggcttggc aagtttcata ccgaattcga tgcgctatgg gatttcgcgc cgcacgatct ttcgatgatc cttgccatca ccggcgagga gccgaacgtg gtgcgcggcg aaggcgttgc tattctcgat catctgaacg atttcgcgca tcttcacatg gaatttccga gcggaattcg cggccatctt tttgcttcgc gtctgaacgc ttatcgcgaa cgtcgcctca gtgtcaccgg caccaagggc atggctgttt tcgacgatgg cgaagcctgg gagcggaagc ttgcccttta caggcatgag gtctggcggg aaaacgaccg ctgggctttc aagtccgccg atccggtcta tatacaaacc gaagaaggta tgccgctgac gcgcgaattg cagcatttca tgcattgcat cgaaacgcgt gaaacgccgc gaaccgacgg caaggaagca atcagtgtcc tgcgcattct caccgaaggc acggtgcggc atccggccta agtga
Expressed Protein Sequence
MAHHHHHHMA PRIAVLGCGY WGGNHIRTLK SLGALQAVSD SNAEKAERFA SEFNVPSIPV EELFIHPDID GIVLALPPQL HAQYAMEAVK NGKDVLVEKP IALDIPVALA EVEAARENGR VFMVGHVLRF HPAFEKLLDM VQSGELGDIR YVHSHRVGLG KFHTEFDALW DFAPHDLSMI LAITGEEPNV VRGEGVAILD HLNDFAHLHM EFPSGIRGHL FASRLNAYRE RRLSVTGTKG MAVFDDGEAW ERKLALYRHE VWRENDRWAF KSADPVYIQT EEGMPLTREL QHFMHCIETR ETPRTDGKEA ISVLRILTEG TVRHPA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAC CTCGTATCGC GGTTTTGGGT TGCGGCTATT GGGGCGGCAA TCACATTCGT ACCCTGAAGA GTCTCGGCGC CCTTCAGGCA GTTTCAGACT CCAACGCGGA AAAAGCGGAA CGATTCGCTT CCGAATTCAA CGTTCCCAGC ATTCCAGTCG AGGAACTCTT CATCCATCCG GATATTGATG GCATCGTGCT GGCGCTGCCG CCGCAGCTTC ATGCGCAATA TGCCATGGAA GCGGTGAAGA ACGGCAAGGA TGTTCTGGTT GAAAAGCCGA TTGCGCTCGA TATTCCCGTA GCGCTTGCCG AAGTTGAGGC CGCGCGCGAA AATGGCCGCG TGTTCATGGT CGGCCATGTG CTCCGCTTCC ATCCGGCTTT TGAAAAGCTT CTCGACATGG TGCAAAGCGG CGAACTTGGC GATATCCGCT ATGTGCATTC GCACCGCGTC GGGCTTGGCA AGTTTCATAC CGAATTCGAT GCGCTATGGG ATTTCGCGCC GCACGATCTT TCGATGATCC TTGCCATCAC CGGCGAGGAG CCGAACGTGG TGCGCGGCGA AGGCGTTGCT ATTCTCGATC ATCTGAACGA TTTCGCGCAT CTTCACATGG AATTTCCGAG CGGAATTCGC GGCCATCTTT TTGCTTCGCG TCTGAACGCT TATCGCGAAC GTCGCCTCAG TGTCACCGGC ACCAAGGGCA TGGCTGTTTT CGACGATGGC GAAGCCTGGG AGCGGAAGCT TGCCCTTTAC AGGCATGAGG TCTGGCGGGA AAACGACCGC TGGGCTTTCA AGTCCGCCGA TCCGGTCTAT ATACAAACCG AAGAAGGTAT GCCGCTGACG CGCGAATTGC AGCATTTCAT GCATTGCATC GAAACGCGTG AAACGCCGCG AACCGACGGC AAGGAAGCGA TCAGTGTCCT GCGCATTCTC ACCGAAGGCA CGGTGCGGCA TCCGGCCTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT
Details for BrabA.18948.a.B1.PW37688
PURIFICATION DATe: 4/6/2015
CONCENTRATION: 32mg/ml
OBSERVED MW: 37kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 21
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA PRIAVLGCGY WGGNHIRTLK SLGALQAVSD SNAEKAERFA SEFNVPSIPV EELFIHPDID GIVLALPPQL HAQYAMEAVK NGKDVLVEKP IALDIPVALA EVEAARENGR VFMVGHVLRF HPAFEKLLDM VQSGELGDIR YVHSHRVGLG KFHTEFDALW DFAPHDLSMI LAITGEEPNV VRGEGVAILD HLNDFAHLHM EFPSGIRGHL FASRLNAYRE RRLSVTGTKG MAVFDDGEAW ERKLALYRHE VWRENDRWAF KSADPVYIQT EEGMPLTREL QHFMHCIETR ETPRTDGKEA ISVLRILTEG TVRHPA
Validated NT Sequence
atggctcacc accaccacca ccatatggca cctcgtatcg cggttttggg ttgcggctat tggggcggca atcacattcg taccctgaag agtctcggcg cccttcaggc agtttcagac tccaacgcgg aaaaagcgga acgattcgct tccgaattca acgttcccag cattccagtc gaggaactct tcatccatcc ggatattgat ggcatcgtgc tggcgctgcc gccgcagctt catgcgcaat atgccatgga agcggtgaag aacggcaagg atgttctggt tgaaaagccg attgcgcttg atattcccgt agcgcttgcc gaagttgagg ccgcgcgcga aaatggccgc gtgttcatgg tcggccatgt gctccgcttc catccggctt ttgaaaagct tctcgacatg gtgcaaagcg gcgaacttgg cgatatccgc tatgtgcatt cgcaccgcgt cgggcttggc aagtttcata ccgaattcga tgcgctatgg gatttcgcgc cgcacgatct ttcgatgatc cttgccatca ccggcgagga gccgaacgtg gtgcgcggcg aaggcgttgc tattctcgat catctgaacg atttcgcgca tcttcacatg gaatttccga gcggaattcg cggccatctt tttgcttcgc gtctgaacgc ttatcgcgaa cgtcgcctca gtgtcaccgg caccaagggc atggctgttt tcgacgatgg cgaagcctgg gagcggaagc ttgcccttta caggcatgag gtctggcggg aaaacgaccg ctgggctttc aagtccgccg atccggtcta tatacaaacc gaagaaggta tgccgctgac gcgcgaattg cagcatttca tgcattgcat cgaaacgcgt gaaacgccgc gaaccgacgg caaggaagca atcagtgtcc tgcgcattct caccgaaggc acggtgcggc atccggccta agtga
Expressed Protein Sequence
MAHHHHHHMA PRIAVLGCGY WGGNHIRTLK SLGALQAVSD SNAEKAERFA SEFNVPSIPV EELFIHPDID GIVLALPPQL HAQYAMEAVK NGKDVLVEKP IALDIPVALA EVEAARENGR VFMVGHVLRF HPAFEKLLDM VQSGELGDIR YVHSHRVGLG KFHTEFDALW DFAPHDLSMI LAITGEEPNV VRGEGVAILD HLNDFAHLHM EFPSGIRGHL FASRLNAYRE RRLSVTGTKG MAVFDDGEAW ERKLALYRHE VWRENDRWAF KSADPVYIQT EEGMPLTREL QHFMHCIETR ETPRTDGKEA ISVLRILTEG TVRHPA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAC CTCGTATCGC GGTTTTGGGT TGCGGCTATT GGGGCGGCAA TCACATTCGT ACCCTGAAGA GTCTCGGCGC CCTTCAGGCA GTTTCAGACT CCAACGCGGA AAAAGCGGAA CGATTCGCTT CCGAATTCAA CGTTCCCAGC ATTCCAGTCG AGGAACTCTT CATCCATCCG GATATTGATG GCATCGTGCT GGCGCTGCCG CCGCAGCTTC ATGCGCAATA TGCCATGGAA GCGGTGAAGA ACGGCAAGGA TGTTCTGGTT GAAAAGCCGA TTGCGCTCGA TATTCCCGTA GCGCTTGCCG AAGTTGAGGC CGCGCGCGAA AATGGCCGCG TGTTCATGGT CGGCCATGTG CTCCGCTTCC ATCCGGCTTT TGAAAAGCTT CTCGACATGG TGCAAAGCGG CGAACTTGGC GATATCCGCT ATGTGCATTC GCACCGCGTC GGGCTTGGCA AGTTTCATAC CGAATTCGAT GCGCTATGGG ATTTCGCGCC GCACGATCTT TCGATGATCC TTGCCATCAC CGGCGAGGAG CCGAACGTGG TGCGCGGCGA AGGCGTTGCT ATTCTCGATC ATCTGAACGA TTTCGCGCAT CTTCACATGG AATTTCCGAG CGGAATTCGC GGCCATCTTT TTGCTTCGCG TCTGAACGCT TATCGCGAAC GTCGCCTCAG TGTCACCGGC ACCAAGGGCA TGGCTGTTTT CGACGATGGC GAAGCCTGGG AGCGGAAGCT TGCCCTTTAC AGGCATGAGG TCTGGCGGGA AAACGACCGC TGGGCTTTCA AGTCCGCCGA TCCGGTCTAT ATACAAACCG AAGAAGGTAT GCCGCTGACG CGCGAATTGC AGCATTTCAT GCATTGCATC GAAACGCGTG AAACGCCGCG AACCGACGGC AAGGAAGCGA TCAGTGTCCT GCGCATTCTC ACCGAAGGCA CGGTGCGGCA TCCGGCCTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT