SSGCID: Target Page

BrabA.18830.a

BspG VirB type IV secretion effector protein

CENTER ID:	BrabA.18830.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.18830.a.B1.GE37972	full length		1	202

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.290
RefSeq:	YP_413751.1
UniProt:	Q2YPC6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSQPANSSAE VHAFPRRHGF NAFEQAKQEL AAFKFSLFET VAADPLLRAG QCLNLIIVYA SLVTIDKKTL QATPAYASNG RVFVRAGVRS HHTAVKARRL LEQHGYLIPV SRRNGITVYR IENPHHERVQ MQIQELEECQ REIKTEKMYA ISRNPAWQIM PTLTVQGWQK MPTLKIVRMA IIAHQGWQKV PTKVGRKCHQ LP

NT Sequence

atgagccaac cagccaattc atccgccgag gttcatgcct tcccccgtcg ccatggcttc aatgccttcg agcaggcaaa gcaggaactg gccgcgttca agttctcatt gttcgaaacg gttgctgccg atccgctttt gcgtgcgggt cagtgtctca atctgattat cgtctatgcg tccctcgtaa ctatcgacaa gaagacgctc caggcgacac cggcttatgc atcgaacgga cgagtattcg ttcgcgctgg tgttcggtcg catcacacgg ccgtcaaggc gcgcaggctt cttgaacagc atggttatct cattccggtc agccggagga atggcatcac ggtctaccgg atcgagaacc cacatcatga acgcgttcag atgcaaattc aggaattgga agagtgccaa cgtgagataa aaaccgaaaa aatgtacgca ataagccgca atccagcatg gcaaataatg cccaccttga ccgtacaggg gtggcagaaa atgcccaccc tgaaaatcgt aaggatggca ataattgccc accaaggatg gcagaaagtg ccgaccaagg tgggcagaaa atgccaccaa ttaccttaa

Details for BrabA.18830.a.B1.GE37972

HARVESTED ON:	5/12/2014
SEQUENCED ON:	5/7/2014
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMX QPANSSAEVH AFPRRHGFNA FEQAKQELAA FKFSLFETVA ADPLLRAGQC LNLIIVYASL VTIDKKTLQA TPAYASNGRV FVRAGVRSHH TAVKARRLLE QHGYLIPVSR RNGITVYRIE NPHHERVQMQ IQELEECQRE IKTEKMCAIS RNPAWQIMPT LTVQGWQKMP TLKIVRMAII AHQGWQKVPT KVGRKCHQLP

Validated NT Sequence

atggctcacc accaccacca ccatatganc caaccagcca attcatccgc cgaggttcat gccttccccc gtcgccatgg cttcaatgcc ttcgagcagg caaagcagga actggccgcg ttcaagttct cattgttcga aacggttgct gccgatccgc ttttgcgtgc gggtcagtgt ctcaatctga ttatcgtcta tgcgtccctc gtaactatcg acaagaagac gctccaggcg acaccggctt atgcatcgaa cggacgagta ttcgttcgcg ctggtgttcg gtcgcatcac acggccgtca aggcgcgcag gcttcttgaa cagcatggtt atctcattcc ggtcagccgg aggaatggca tcacggtcta ccggatcgag aacccacatc atgaacgcgt tcagatgcaa attcaggaat tggaagagtg ccaacgtgag ataaaaaccg aaaaaatgtg cgcaataagc cgcaatccag catggcaaat aatgcccacc ttgaccgtac aggggtggca gaaaatgccc accctgaaaa tcgtaaggat ggcaataatt gcccaccaag gatggcagaa agtgccgacc aaggtgggca gaaaatgcca ccaattacct taagtgagta agat

Expected Protein Sequence

MAHHHHHHMS QPANSSAEVH AFPRRHGFNA FEQAKQELAA FKFSLFETVA ADPLLRAGQC LNLIIVYASL VTIDKKTLQA TPAYASNGRV FVRAGVRSHH TAVKARRLLE QHGYLIPVSR RNGITVYRIE NPHHERVQMQ IQELEECQRE IKTEKMYAIS RNPAWQIMPT LTVQGWQKMP TLKIVRMAII AHQGWQKVPT KVGRKCHQLP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc aaccagccaa ttcatccgcc gaggttcatg ccttcccccg tcgccatggc ttcaatgcct tcgagcaggc aaagcaggaa ctggccgcgt tcaagttctc attgttcgaa acggttgctg ccgatccgct tttgcgtgcg ggtcagtgtc tcaatctgat tatcgtctat gcgtccctcg taactatcga caagaagacg ctccaggcga caccggctta tgcatcgaac ggacgagtat tcgttcgcgc tggtgttcgg tcgcatcaca cggccgtcaa ggcgcgcagg cttcttgaac agcatggtta tctcattccg gtcagccgga ggaatggcat cacggtctac cggatcgaga acccacatca tgaacgcgtt cagatgcaaa ttcaggaatt ggaagagtgc caacgtgaga taaaaaccga aaaaatgtac gcaataagcc gcaatccagc atggcaaata atgcccacct tgaccgtaca ggggtggcag aaaatgccca ccctgaaaat cgtaaggatg gcaataattg cccaccaagg atggcagaaa gtgccgacca aggtgggcag aaaatgccac caattacctt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat