SSGCID: Target Page

BrabA.18828.a

BspF VirB type IV secretion effector protein

CENTER ID:	BrabA.18828.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.18828.a.B1.GE37971	full length		1	428

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.18828.a.B1.PW37524	full length		1	428

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.2027
RefSeq:	YP_415285.1
UniProt:	Q2YLT8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAAKPLLEES AGGNAAHLVS QLSDSSAVIE AAKKMEESLH SRDIPRKLAI YGAAAGFELR DELDHLSNRT IEPNIFFNAR FLAPAMPRLE DREVRFMVMR DENETRSRLR FVMPYTVERP GLPLSVPVIR SWATPFGPQG TPLIDHDNPV GVVEDLFDIL ARRHIKLPEV LVLPEMRANG AAARLIRSVA IGRQLPVQSI EHKERPFLES KLDGKTYIKE AIGAHHRHDY ERLWRRLAEQ GELVHKVART PDEVRHAFEH FLTLEARGWK GRRGTAMAVD RFRAAFAREA VNNLAECDCV RIHTLELDGR VIAILIVFTV SGEAWTWKTA YDETFHAWSP GVLLMIEVVK NHLDDPNIAR TDSCAVPDHP VMTRLFRERE TMETLVVGLH PGADRLVRQA ASQIHLYQRT RNLARIIRNR LRSFTEHK

NT Sequence

atggctgcaa aacctttgct tgaggaatca gcaggcggaa acgccgccca tcttgtttcg caactttccg acagcagcgc cgtgatcgaa gcggcgaaga agatggagga aagcctgcac agccgcgata ttccgcgcaa gcttgccatt tatggcgcag cagccggttt tgaattgcgt gatgagctgg accatctgag caatcgcacc atagagccga acatcttctt caacgcacgt tttctggcgc ctgccatgcc acgccttgaa gaccgtgaag ttcgcttcat ggtcatgcgc gacgaaaatg aaacccgaag ccgtctgcgc tttgtgatgc cctatacggt ggaacgcccc ggcctgccac tttcggttcc cgtgattcgc tcctgggcca cccccttcgg cccgcagggc acgccgctca tcgaccatga caacccggtc ggggtggttg aagacctgtt cgacattctg gcgcgcaggc acatcaagct gcccgaagta ctggttttgc cggaaatgcg tgccaatgga gcagccgcca ggctgatccg ctcggtcgcc atcgggcgcc aattgccggt tcaatccatc gagcacaagg aacgtccctt ccttgaaagc aagctcgatg gcaagaccta tataaaggaa gccatcgggg cgcatcatcg ccatgactat gaacgattgt ggcgccgcct tgccgagcag ggcgaactcg ttcacaaggt tgcccgcacg cccgatgagg tgcgccatgc ctttgaacat ttcctcacac tggaggcgcg cggctggaag ggacgtcgcg gcacagccat ggctgttgac cgttttcgtg ccgctttcgc gcgcgaagcc gtcaacaatc ttgccgaatg cgactgtgta cgcattcaca cgctggaact tgatggcagg gtcatcgcaa tcctcatcgt cttcacggta tcaggcgaag cctggacctg gaaaaccgcc tatgacgaga catttcatgc ctggtcgccc ggcgttctcc tgatgatcga agtggtgaag aaccacctgg acgaccccaa tatcgcccgc accgattcct gcgccgtgcc agaccatccc gtcatgacgc gcctcttccg tgaacgcgag actatggaaa cgcttgtcgt cggccttcac cccggcgcgg atcggctggt gcggcaggcg gcatcgcaga ttcacctcta tcagcgcacc cgcaatctcg cgcgcatcat ccgcaaccgg ctgcgcagtt tcaccgagca taaataa

Details for BrabA.18828.a.B1.GE37971

HARVESTED ON:	5/12/2014
SEQUENCED ON:	5/4/2014
EXPECTED MW:	50kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	90

Validated AA Sequence

MEESLHSRDI PRKLAIYGAA AGFELRDELD HLSNRTIEPN IFFNARFLAP AMPRLEDREV RFMVMRDENE TRSRLRFVMP YTVERPGLPL SVPVIRSWAT PFGPQGTPLI DHDNPVGVVE DLFDILARRH IKLPEVLVLP EMRANGAAAR LIRSVAIGRQ LPVQSIEHKE RPFLESKLDG KTYIKEAIGA HHRHDYERLW RRLAEQGELV HKVARTPDEV RHAFEHFLTL VARGWKGRRG TAMAVDRFRA AFAREAVNNL AECDCVRIHT LELDGRVIAI LIVFTVSGEA XTWKTAXDET FHAWSPGVLX MIEEVKNHLX DPNIARTDSX AVPDHPVMTR LFRERETMET LVVGLHPGAD RLVRQAASQI HLYQRTRNLA RIIRNRXRSF TEHK

Validated NT Sequence

atcttactca cttatttatg ctcggtgaaa ctgcgcancc ggttgcggat gatgcgcgcg agattgcggg tgcgctgata gaggtgaatc tgcgatgccg cctgccgcac cagccgatcc gcgccggggt gaaggccgac nacnagcgtt tccatagtct cncgttcncg gaanaggcgc gtcatgacgg gatggtctgg cacggcncat gaatcngtgc gggcgatatt ggggtcntcc aggtggttct tcacctcttc gatcatcnng agaacgccgg gcgaccaggc atgaaatgtc tcgtcntagg cggttttcca ggtccnggct tcgcctgata ccgtgaagac gatgaggatt gcgatgaccc tgccatcaag ttccagcgtg tgaatgcgta cacagtcgca ttcggcaaga ttgttgacgg cttcgcgcgc gaaagcggca cgaaaacggt caacagccat ggctgtgccg cgacgtccct tccagccgcg cgccaccagt gtgaggaaat gttcaaaggc atggcgcacc tcatcgggcg tgcgggcaac cttgtgaacg agttcgccct gctcggcaag gcggcgccac aatcgttcat agtcatggcg atgatgcgcc ccgatggctt cctttatata ggtcttgcca tcgagcttgc tttcaaggaa gggacgttcc ttgtgctcga tggattgaac cggcaattgg cgcccgatgg cgaccgagcg gatcagcctg gcggctgctc cattggcacg catttccggc aaaaccagta cttcgggcag cttgatgtgc ctgcgcgcca gaatgtcgaa caggtcttca accaccccga ccgggttgtc atggtcgatg agcggcgtgc cctgcgggcc gaagggggtg gcccaggagc gaatcacggg aaccgaaagt ggcaggccgg ggcgttccac cgtatagggc atcacaaagc gcagacggct tcgggtttca ttttcgtcgc gcatgaccat gaagcgaact tcacggtctt caaggcgtgg catggcaggc gccagaaaac gtgcgttgaa gaagatgttc ggctctatgg tgcgattgct cagatggtcc agctcatcac gcaattcaaa accggctgct gcgccataaa tggcaagctt gcgcggaata tcgcggctgt gcaggctttc ctccatcttc ttcgccgctt cgatcacggc gctgctgtcg gaaagttgcg aaacaagatg ggcggcgttt ccgcctgctg attcctcaag caaa

Expected Protein Sequence

MAHHHHHHMA AKPLLEESAG GNAAHLVSQL SDSSAVIEAA KKMEESLHSR DIPRKLAIYG AAAGFELRDE LDHLSNRTIE PNIFFNARFL APAMPRLEDR EVRFMVMRDE NETRSRLRFV MPYTVERPGL PLSVPVIRSW ATPFGPQGTP LIDHDNPVGV VEDLFDILAR RHIKLPEVLV LPEMRANGAA ARLIRSVAIG RQLPVQSIEH KERPFLESKL DGKTYIKEAI GAHHRHDYER LWRRLAEQGE LVHKVARTPD EVRHAFEHFL TLEARGWKGR RGTAMAVDRF RAAFAREAVN NLAECDCVRI HTLELDGRVI AILIVFTVSG EAWTWKTAYD ETFHAWSPGV LLMIEVVKNH LDDPNIARTD SCAVPDHPVM TRLFRERETM ETLVVGLHPG ADRLVRQAAS QIHLYQRTRN LARIIRNRLR SFTEHK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg caaaaccttt gcttgaggaa tcagcaggcg gaaacgccgc ccatcttgtt tcgcaacttt ccgacagcag cgccgtgatc gaagcggcga agaagatgga ggaaagcctg cacagccgcg atattccgcg caagcttgcc atttatggcg cagcagccgg ttttgaattg cgtgatgagc tggaccatct gagcaatcgc accatagagc cgaacatctt cttcaacgca cgttttctgg cgcctgccat gccacgcctt gaagaccgtg aagttcgctt catggtcatg cgcgacgaaa atgaaacccg aagccgtctg cgctttgtga tgccctatac ggtggaacgc cccggcctgc cactttcggt tcccgtgatt cgctcctggg ccaccccctt cggcccgcag ggcacgccgc tcatcgacca tgacaacccg gtcggggtgg ttgaagacct gttcgacatt ctggcgcgca ggcacatcaa gctgcccgaa gtactggttt tgccggaaat gcgtgccaat ggagcagccg ccaggctgat ccgctcggtc gccatcgggc gccaattgcc ggttcaatcc atcgagcaca aggaacgtcc cttccttgaa agcaagctcg atggcaagac ctatataaag gaagccatcg gggcgcatca tcgccatgac tatgaacgat tgtggcgccg ccttgccgag cagggcgaac tcgttcacaa ggttgcccgc acgcccgatg aggtgcgcca tgcctttgaa catttcctca cactggaggc gcgcggctgg aagggacgtc gcggcacagc catggctgtt gaccgttttc gtgccgcttt cgcgcgcgaa gccgtcaaca atcttgccga atgcgactgt gtacgcattc acacgctgga acttgatggc agggtcatcg caatcctcat cgtcttcacg gtatcaggcg aagcctggac ctggaaaacc gcctatgacg agacatttca tgcctggtcg cccggcgttc tcctgatgat cgaagtggtg aagaaccacc tggacgaccc caatatcgcc cgcaccgatt cctgcgccgt gccagaccat cccgtcatga cgcgcctctt ccgtgaacgc gagactatgg aaacgcttgt cgtcggcctt caccccggcg cggatcggct ggtgcggcag gcggcatcgc agattcacct ctatcagcgc acccgcaatc tcgcgcgcat catccgcaac cggctgcgca gtttcaccga gcataaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for BrabA.18828.a.B1.PW37524

PURIFICATION DATe:	7/8/2014
CONCENTRATION:	3.14mg/ml
OBSERVED MW:	48kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	90

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTG CAAAACCTTT GCTTGAGGAA TCAGCAGGCG GAAACGCCGC CCATCTTGTT TCGCAACTTT CCGACAGCAG CGCCGTGATC GAAGCGGCGA AGAAGATGGA GGAAAGCCTG CACAGCCGCG ATATTCCGCG CAAGCTTGCC ATTTATGGCG CAGCAGCCGG TTTTGAATTG CGTGATGAGC TGGACCATCT GAGCAATCGC ACCATAGAGC CGAACATCTT CTTCAACGCA CGTTTTCTGG CGCCTGCCAT GCCACGCCTT GAAGACCGTG AAGTTCGCTT CATGGTCATG CGCGACGAAA ATGAAACCCG AAGCCGTCTG CGCTTTGTGA TGCCCTATAC GGTGGAACGC CCCGGCCTGC CACTTTCGGT TCCCGTGATT CGCTCCTGGG CCACCCCCTT CGGCCCGCAG GGCACGCCGC TCATCGACCA TGACAACCCG GTCGGGGTGG TTGAAGACCT GTTCGACATT CTGGCGCGCA GGCACATCAA GCTGCCCGAA GTACTGGTTT TGCCGGAAAT GCGTGCCAAT GGAGCAGCCG CCAGGCTGAT CCGCTCGGTC GCCATCGGGC GCCAATTGCC GGTTCAATCC ATCGAGCACA AGGAACGTCC CTTCCTTGAA AGCAAGCTCG ATGGCAAGAC CTATATAAAG GAAGCCATCG GGGCGCATCA TCGCCATGAC TATGAACGAT TGTGGCGCCG CCTTGCCGAG CAGGGCGAAC TCGTTCACAA GGTTGCCCGC ACGCCCGATG AGGTGCGCCA TGCCTTTGAA CATTTCCTCA CACTGGAGGC GCGCGGCTGG AAGGGACGTC GCGGCACAGC CATGGCTGTT GACCGTTTTC GTGCCGCTTT CGCGCGCGAA GCCGTCAACA ATCTTGCCGA ATGCGACTGT GTACGCATTC ACACGCTGGA ACTTGATGGC AGGGTCATCG CAATCCTCAT CGTCTTCACG GTATCAGGCG AAGCCTGGAC CTGGAAAACC GCCTATGACG AGACATTTCA TGCCTGGTCG CCCGGCGTTC TCCTGATGAT CGAAGTGGTG AAGAACCACC TGGACGACCC CAATATCGCC CGCACCGATT CCTGCGCCGT GCCAGACCAT CCCGTCATGA CGCGCCTCTT CCGTGAACGC GAGACTATGG AAACGCTTGT CGTCGGCCTT CACCCCGGCG CGGATCGGCT GGTGCGGCAG GCGGCATCGC AGATTCACCT CTATCAGCGC ACCCGCAATC TCGCGCGCAT CATCCGCAAC CGGCTGCGCA GTTTCACCGA GCATAAATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT