SSGCID: Target Page

BrabA.18827.a

BspI VirB type IV secretion effector protein

CENTER ID:	BrabA.18827.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.18827.a.B1.GE37970	full length		1	224

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.1937
RefSeq:	YP_415209.1
UniProt:	Q2YLL0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDLIHIHED DWGMRNLFPL AAFSEVKEDI AKSATAAAKH QDASGFGYTD VYLIEPPSIS YADVGLLVSD AEGVLLPILP RVQQFRATSF QGMTSGKQDS YGTYQDDTSC FGLGRHCYLK LDKKGPLVEG IWFGLDTDDV DAIGRLRMAI EAIDALVPSV IADYFLDISG PVGADGVLDS YFEAFQLQHL KAKQAVQEFQ AKYRRQENML DKLRKLVAFL GRFR

NT Sequence

atgactgacc tgattcacat acatgaagac gattggggaa tgcgtaatct ttttcccctg gccgcctttt ctgaagtgaa agaagatatt gccaaatctg cgaccgccgc agcgaaacac caggacgctt cgggttttgg ctatacggat gtttatttga tagagccacc ttcgatcagt tatgccgatg tcgggttgtt agtctctgat gcggaaggcg tgttacttcc aatcctgccg cgcgtgcaac agttccgcgc cacatcgttt caggggatga ctagcggaaa acaggattca tacggcacat atcaggatga cacgtcgtgc tttggtctag gacgccactg ctatctcaaa ctcgacaaaa agggccctct tgttgaagga atctggtttg gtctggatac cgatgatgta gatgcaattg gccggctgcg tatggctatc gaagccattg atgcgttggt accgagcgtt attgccgatt attttctcga tatctccggc ccggtcggtg cggatggagt cctagacagc tattttgagg cttttcaatt gcaacacctc aaagccaagc aggcagtaca ggaatttcag gccaagtatc gacggcagga aaatatgctg gacaaactgc gcaagcttgt ggcgtttttg ggccgctttc gctga

Details for BrabA.18827.a.B1.GE37970

HARVESTED ON:	5/12/2014
SEQUENCED ON:	5/4/2014
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT DLIHIHEDDW GMRNLFPLAA FSEVKEDIAK SATAAAKHQD ASGFGYTDVY LIEPPSISYA DVGLLVSDAE GVLLPILPRV QQFRATSFQG MTSGKQDSYG TYQDDTSCFG LGRHCYLKLD KKGPLVEGIW FGLDTDDVDA IGRLRMAIEA IDALVPSVIA DYFLDISGPV GADGVLDSYF EAFQLQHLKA KQAVQEFQAK YRRQENMLDK LRKLVAFLGR FR

Validated NT Sequence

atcttactca cttagcgaaa gcggcccaaa aacgccacaa gcttgcgcag tttgtccagc atattttcct gccgtcgata cttggcctga aattcctgta ctgcctgctt ggctttgagg tgttgcaatt gaaaagcctc aaaatagctg tctaggactc catccgcacc gaccgggccg gagatatcga gaaaataatc ggcaataacg ctcggtacca acgcatcaat ggcttcgata gccatacgca gccggccaat tgcatctaca tcatcggtat ccagaccaaa ccagattcct tcaacaagag ggcccttttt gtcgagtttg agatagcagt ggcgtcctag accaaagcac gacgtgtcat cctgatatgt gccgtatgaa tcctgttttc cgctagtcat cccctgaaac gatgtggcgc ggaactgttg cacgcgcggc aggattggaa gtaacacgcc ttccgcatca gagactaaca acccgacatc ggcataactg atcgaaggtg gctctatcaa ataaacatcc gtatagccaa aacccgaagc gtcctggtgt ttcgctgcgg cggtcgcaga tttggcaata tcttctttca cttcagaaaa ggcggccagg ggaaaaagat tacgcattcc ccaatcgtct tcatgtatgt gaatcaggtc agtcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg acctgattca catacatgaa gacgattggg gaatgcgtaa tctttttccc ctggccgcct tttctgaagt gaaagaagat attgccaaat ctgcgaccgc cgcagcgaaa caccaggacg cttcgggttt tggctatacg gatgtttatt tgatagagcc accttcgatc agttatgccg atgtcgggtt gttagtctct gatgcggaag gcgtgttact tccaatcctg ccgcgcgtgc aacagttccg cgccacatcg tttcagggga tgactagcgg aaaacaggat tcatacggca catatcagga tgacacgtcg tgctttggtc taggacgcca ctgctatctc aaactcgaca aaaagggccc tcttgttgaa ggaatctggt ttggtctgga taccgatgat gtagatgcaa ttggccggct gcgtatggct atcgaagcca ttgatgcgtt ggtaccgagc gttattgccg attattttct cgatatctcc ggcccggtcg gtgcggatgg agtcctagac agctattttg aggcttttca attgcaacac ctcaaagcca agcaggcagt acaggaattt caggccaagt atcgacggca ggaaaatatg ctggacaaac tgcgcaagct tgtggcgttt ttgggccgct ttcgctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat