BrabA.18180.a
Galactonate dehydratase (EC 4.2.1.6)
| CENTER ID: | BrabA.18180.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.18180.a.B1.GE37888 | Full length( BrabA.18180.a ) | 1 | 601 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.18180.a.B1.PS02071 | Full length( BrabA.18180.a ) | 1 | 601 |
Structures
7M3K
DEPOSITED: 3/18/2021
DETERMINATION: XRay
CLONE: BrabA.18180.a.B1.GE37888
PROTEIN: BrabA.18180.a.B1.PS02071
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|359391.4.peg.2586 |
| RefSeq: | YP_418507.1 |
| UniProt: | Q2YJ21 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNKPVTPKTP KLRSRAWFDN PDDVDMTALY LERYMNYGLS QEELQSGRPI IGIAQTGSDL SPCNRHHLEL AKRVRDGVRE AGGIVIEFPV HPIQETGKRP TAGLDRNLAY LGLVEVLYGY PLDGVVLTIG CDKTTPACLM AAATVNIPAI ALSVGPMLNG WFRGERTGSG TIVWKARELL AKGEIDYQGF VKLVASSAPS TGYCNTMGTA TTMNSLAEAL GMQLPGSAAI PAPYRDRQEV AYLTGRRIVE MVHEDLKPSD ILTKEAFINA IRVNSAIGGS TNAPIHLNAL ARHIGVELTV DDWQKYGEEI PLLVNLQPAG EYLGEDYYHA GGVPAVVNQL MGQGLIHEDA ITVNGKTIGE NCKNATIEDG NVIKTYDQPL KKHAGFRVLR GNLFSSAIMK LSVISDEFRN RYLSDAKDPN AFEGKAVVFD GPEDYHHRID DPALEIDEHT VLFMRGAGPI GYPGAAEVVN MRAPDYLLKK GITSLPCIGD GRQSGTSGSP SILNASPEAA AGGGLAILKT GDRVRIDLGR GTADILISDE ELAERRKALE AVGGYKYPES QTPWQEIQRA VIGQMETGAV LENAVKYQDI AHTRGLPRDN H
NT Sequence
ATGAACAAGC CGGTTACGCC AAAAACACCC AAATTGCGGT CACGCGCATG GTTCGACAAT CCCGACGATG TCGATATGAC GGCGCTCTAT CTTGAGCGTT ACATGAATTA CGGGCTGAGC CAGGAAGAGC TGCAATCCGG CCGTCCCATC ATCGGTATTG CGCAAACCGG TTCTGATCTT TCGCCCTGCA ACCGGCATCA TCTGGAACTG GCCAAGCGTG TGAGGGATGG AGTTCGCGAG GCGGGTGGCA TCGTCATCGA ATTTCCGGTT CATCCCATTC AGGAAACCGG CAAGCGCCCC ACTGCGGGTC TCGACCGCAA TCTGGCCTAT CTTGGTCTGG TCGAAGTGCT TTACGGCTAT CCGCTCGATG GTGTGGTGCT GACCATCGGC TGCGACAAGA CCACGCCCGC CTGCCTGATG GCAGCGGCGA CCGTCAATAT TCCGGCCATC GCGCTGTCGG TCGGGCCGAT GCTGAACGGC TGGTTCCGGG GTGAGCGCAC GGGTTCGGGC ACCATCGTCT GGAAGGCGCG CGAACTGCTT GCCAAGGGCG AAATCGACTA TCAGGGCTTT GTGAAACTGG TGGCTTCTTC GGCTCCATCG ACCGGCTATT GCAACACGAT GGGCACGGCC ACGACCATGA ATTCTCTGGC CGAAGCGCTG GGTATGCAGC TTCCGGGCTC GGCGGCTATC CCCGCACCTT ATCGTGACCG TCAGGAAGTC GCCTATCTGA CGGGGCGGCG TATTGTCGAG ATGGTTCATG AAGACCTGAA ACCATCGGAT ATCCTGACGA AGGAAGCCTT TATCAATGCG ATCCGCGTCA ATTCCGCCAT TGGCGGCTCC ACCAATGCGC CGATCCATCT CAATGCGCTC GCGCGCCATA TCGGTGTGGA ACTGACGGTG GATGACTGGC AGAAATATGG CGAGGAAATT CCGCTTCTGG TCAATCTCCA GCCTGCGGGC GAATATCTGG GCGAAGACTA TTATCACGCA GGCGGTGTGC CAGCGGTGGT CAACCAGTTG ATGGGGCAGG GCCTCATCCA TGAGGACGCA ATCACGGTCA ATGGCAAGAC CATCGGCGAA AATTGCAAGA ATGCGACCAT CGAAGACGGC AATGTCATCA AGACCTACGA TCAGCCGCTC AAGAAACATG CCGGTTTCCG CGTGCTGCGC GGCAATCTGT TCTCGTCGGC CATCATGAAG CTCAGCGTGA TTTCGGACGA ATTCCGCAAT CGCTACCTGT CCGATGCCAA GGATCCCAAT GCCTTTGAAG GCAAGGCGGT CGTGTTCGAT GGGCCGGAGG ATTATCATCA CCGTATTGAT GATCCGGCGC TGGAAATAGA TGAACATACG GTGCTTTTCA TGCGCGGTGC GGGACCGATC GGCTATCCGG GTGCGGCGGA AGTCGTCAAC ATGCGCGCGC CGGATTACCT TTTGAAGAAG GGCATCACGT CGCTGCCCTG TATCGGCGAC GGCCGCCAGT CGGGTACGTC GGGTTCGCCG TCGATCCTCA ATGCCTCGCC GGAAGCGGCG GCAGGCGGGG GGCTTGCCAT TCTCAAGACC GGCGACCGCG TGCGCATCGA TCTTGGCCGC GGGACGGCGG ATATCCTGAT TTCCGATGAG GAATTGGCCG AACGCCGCAA GGCGCTGGAA GCGGTTGGCG GTTATAAATA TCCTGAAAGC CAGACGCCAT GGCAGGAAAT CCAGCGTGCT GTCATCGGCC AGATGGAAAC CGGGGCCGTG CTGGAAAACG CGGTCAAATA TCAGGATATC GCGCATACGC GCGGGCTTCC GCGAGATAAC CAT
Details for BrabA.18180.a.B1.GE37888
| HARVESTED ON: | 4/10/2014 |
| SEQUENCED ON: | 4/8/2014 |
| EXPECTED MW: | 66kDa |
| OBSERVED MW: | 66kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 85 |
| PERCENT COVERAGE: | 67 |
Validated AA Sequence
MAHHHHHHMN KPVTPKTPKL RSRAWFDNPD DVDMTALYLE RYMNYGLSQE ELQSGRPIIG IAQTGSDLSP CNRHHLELAK RVRDGVREAG GIVIEFPVHP IQETGKRPTA GLDRNLAYLG LVEVLYGYPL DGVVLTIGCD KTTPACLMAA ATVNIPAIAL SVGPMLNGWF RGERTGSGTI VWKARELLAK GEIDYQGFVK LVASSAPSTG YCNTMGTATT MNSLAEALGM QLPGSAAIPA PYRDRQEVAY LMGRRIVEMV HEDLKPSDIL TKEAFINAIR VNSAIGGSTN APIHLNALAR HIGVELTVDD WQKYGEEIPL LVNLQPAGEY LGEDYYHAXX CQRWSXVDGA GLIHXXXXGH GRPSAKLQXX TSXXXSSXXD XXLXKHXXFX XAXXXXXRXP XXXXXXXFXX XXX
Validated NT Sequence
gnnnncncta gaatantttt gtttacttta agaaggagat ataccatggc tcaccaccac caccaccata tgaacaagcc ggttacgcca aaaacaccca aattgcggtc acgcgcatgg ttcgacaatc ccgacgatgt cgatatgacg gcgctctatc ttgagcgtta catgaattac gggctgagcc aggaagagct gcaatccggc cgtcccatca tcggtattgc gcaaaccggt tctgatcttt cgccctgcaa ccggcatcat ctggaactgg ccaagcgtgt gagggatgga gttcgcgagg cgggtggcat cgtcatcgaa tttccggttc atcccattca ggaaaccggc aagcgcccca ctgcgggtct cgaccgcaat ctggcctatc ttggtctggt cgaagtgctt tacggctatc cgctcgatgg tgtggtgctg accatcggct gcgacaagac cacgcccgcc tgcctgatgg cagcggcgac cgtcaatatt ccggccatcg cgctgtcggt cgggccgatg ctgaacggct ggttccgggg tgagcgcacg ggttcgggca ccatcgtctg gaaggcgcgc gaactgcttg ccaagggcga aatcgactat cagggctttg tgaaactggt ggcttcttcg gctccatcga ccggctattg caacacgatg ggcacggcca cgaccatgaa ttctctggcc gaagcgctgg gtatgcagct tccgggctcg gcggctatcc ccgcacctta tcgtgaccgt caggaagtcg cctatctgat ggggcggcgt attgtcgaga tggttcatga agacctgaaa ccatcggata tcctgacgaa ggaagccttt atcaatgcga tccgcgtcaa ttccgccatt ggcggctcca ccaatgcgcc gatccatctc aatgcgctcg cgcgccatat cggtgtggaa ctgacggtgg atgactggca gaaatatggc gaggaaattc cgcttctggt caatctccag cctgcgggcg aatatctggg cgaagactat tatcacgcnn cgntgtgcca gcggtggtca ncagttgatg gggcnggcct catccatgan ncgcancncg gtcatggcag accatcggcg aaattgcaga ngnngacatc nanangnann tcatcanacn acgatcancn nctcaanaaa catgncnggt ttncnngngc tgnnnnnnnn gnnnctcgnn ngccantcnn gaancnnann nnngnatttc nnnnngnnnn nncgcnc
Expected Protein Sequence
MAHHHHHHMN KPVTPKTPKL RSRAWFDNPD DVDMTALYLE RYMNYGLSQE ELQSGRPIIG IAQTGSDLSP CNRHHLELAK RVRDGVREAG GIVIEFPVHP IQETGKRPTA GLDRNLAYLG LVEVLYGYPL DGVVLTIGCD KTTPACLMAA ATVNIPAIAL SVGPMLNGWF RGERTGSGTI VWKARELLAK GEIDYQGFVK LVASSAPSTG YCNTMGTATT MNSLAEALGM QLPGSAAIPA PYRDRQEVAY LTGRRIVEMV HEDLKPSDIL TKEAFINAIR VNSAIGGSTN APIHLNALAR HIGVELTVDD WQKYGEEIPL LVNLQPAGEY LGEDYYHAGG VPAVVNQLMG QGLIHEDAIT VNGKTIGENC KNATIEDGNV IKTYDQPLKK HAGFRVLRGN LFSSAIMKLS VISDEFRNRY LSDAKDPNAF EGKAVVFDGP EDYHHRIDDP ALEIDEHTVL FMRGAGPIGY PGAAEVVNMR APDYLLKKGI TSLPCIGDGR QSGTSGSPSI LNASPEAAAG GGLAILKTGD RVRIDLGRGT ADILISDEEL AERRKALEAV GGYKYPESQT PWQEIQRAVI GQMETGAVLE NAVKYQDIAH TRGLPRDNH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca agccggttac gccaaaaaca cccaaattgc ggtcacgcgc atggttcgac aatcccgacg atgtcgatat gacggcgctc tatcttgagc gttacatgaa ttacgggctg agccaggaag agctgcaatc cggccgtccc atcatcggta ttgcgcaaac cggttctgat ctttcgccct gcaaccggca tcatctggaa ctggccaagc gtgtgaggga tggagttcgc gaggcgggtg gcatcgtcat cgaatttccg gttcatccca ttcaggaaac cggcaagcgc cccactgcgg gtctcgaccg caatctggcc tatcttggtc tggtcgaagt gctttacggc tatccgctcg atggtgtggt gctgaccatc ggctgcgaca agaccacgcc cgcctgcctg atggcagcgg cgaccgtcaa tattccggcc atcgcgctgt cggtcgggcc gatgctgaac ggctggttcc ggggtgagcg cacgggttcg ggcaccatcg tctggaaggc gcgcgaactg cttgccaagg gcgaaatcga ctatcagggc tttgtgaaac tggtggcttc ttcggctcca tcgaccggct attgcaacac gatgggcacg gccacgacca tgaattctct ggccgaagcg ctgggtatgc agcttccggg ctcggcggct atccccgcac cttatcgtga ccgtcaggaa gtcgcctatc tgacggggcg gcgtattgtc gagatggttc atgaagacct gaaaccatcg gatatcctga cgaaggaagc ctttatcaat gcgatccgcg tcaattccgc cattggcggc tccaccaatg cgccgatcca tctcaatgcg ctcgcgcgcc atatcggtgt ggaactgacg gtggatgact ggcagaaata tggcgaggaa attccgcttc tggtcaatct ccagcctgcg ggcgaatatc tgggcgaaga ctattatcac gcaggcggtg tgccagcggt ggtcaaccag ttgatggggc agggcctcat ccatgaggac gcaatcacgg tcaatggcaa gaccatcggc gaaaattgca agaatgcgac catcgaagac ggcaatgtca tcaagaccta cgatcagccg ctcaagaaac atgccggttt ccgcgtgctg cgcggcaatc tgttctcgtc ggccatcatg aagctcagcg tgatttcgga cgaattccgc aatcgctacc tgtccgatgc caaggatccc aatgcctttg aaggcaaggc ggtcgtgttc gatgggccgg aggattatca tcaccgtatt gatgatccgg cgctggaaat agatgaacat acggtgcttt tcatgcgcgg tgcgggaccg atcggctatc cgggtgcggc ggaagtcgtc aacatgcgcg cgccggatta ccttttgaag aagggcatca cgtcgctgcc ctgtatcggc gacggccgcc agtcgggtac gtcgggttcg ccgtcgatcc tcaatgcctc gccggaagcg gcggcaggcg gggggcttgc cattctcaag accggcgacc gcgtgcgcat cgatcttggc cgcgggacgg cggatatcct gatttccgat gaggaattgg ccgaacgccg caaggcgctg gaagcggttg gcggttataa atatcctgaa agccagacgc catggcagga aatccagcgt gctgtcatcg gccagatgga aaccggggcc gtgctggaaa acgcggtcaa atatcaggat atcgcgcata cgcgcgggct tccgcgagat aaccattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BrabA.18180.a.B1.PS02071
| PURIFICATION DATe: | 6/3/2014 |
| CONCENTRATION: | 27.12mg/ml |
| OBSERVED MW: | 66kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 85 |
| PERCENT COVERAGE: | 67 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN KPVTPKTPKL RSRAWFDNPD DVDMTALYLE RYMNYGLSQE ELQSGRPIIG IAQTGSDLSP CNRHHLELAK RVRDGVREAG GIVIEFPVHP IQETGKRPTA GLDRNLAYLG LVEVLYGYPL DGVVLTIGCD KTTPACLMAA ATVNIPAIAL SVGPMLNGWF RGERTGSGTI VWKARELLAK GEIDYQGFVK LVASSAPSTG YCNTMGTATT MNSLAEALGM QLPGSAAIPA PYRDRQEVAY LMGRRIVEMV HEDLKPSDIL TKEAFINAIR VNSAIGGSTN APIHLNALAR HIGVELTVDD WQKYGEEIPL LVNLQPAGEY LGEDYYHAXX CQRWSXVDGA GLIHXXXXGH GRPSAKLQXX TSXXXSSXXD XXLXKHXXFX XAXXXXXRXP XXXXXXXFXX XXX
Validated NT Sequence
gnnnncncta gaatantttt gtttacttta agaaggagat ataccatggc tcaccaccac caccaccata tgaacaagcc ggttacgcca aaaacaccca aattgcggtc acgcgcatgg ttcgacaatc ccgacgatgt cgatatgacg gcgctctatc ttgagcgtta catgaattac gggctgagcc aggaagagct gcaatccggc cgtcccatca tcggtattgc gcaaaccggt tctgatcttt cgccctgcaa ccggcatcat ctggaactgg ccaagcgtgt gagggatgga gttcgcgagg cgggtggcat cgtcatcgaa tttccggttc atcccattca ggaaaccggc aagcgcccca ctgcgggtct cgaccgcaat ctggcctatc ttggtctggt cgaagtgctt tacggctatc cgctcgatgg tgtggtgctg accatcggct gcgacaagac cacgcccgcc tgcctgatgg cagcggcgac cgtcaatatt ccggccatcg cgctgtcggt cgggccgatg ctgaacggct ggttccgggg tgagcgcacg ggttcgggca ccatcgtctg gaaggcgcgc gaactgcttg ccaagggcga aatcgactat cagggctttg tgaaactggt ggcttcttcg gctccatcga ccggctattg caacacgatg ggcacggcca cgaccatgaa ttctctggcc gaagcgctgg gtatgcagct tccgggctcg gcggctatcc ccgcacctta tcgtgaccgt caggaagtcg cctatctgat ggggcggcgt attgtcgaga tggttcatga agacctgaaa ccatcggata tcctgacgaa ggaagccttt atcaatgcga tccgcgtcaa ttccgccatt ggcggctcca ccaatgcgcc gatccatctc aatgcgctcg cgcgccatat cggtgtggaa ctgacggtgg atgactggca gaaatatggc gaggaaattc cgcttctggt caatctccag cctgcgggcg aatatctggg cgaagactat tatcacgcnn cgntgtgcca gcggtggtca ncagttgatg gggcnggcct catccatgan ncgcancncg gtcatggcag accatcggcg aaattgcaga ngnngacatc nanangnann tcatcanacn acgatcancn nctcaanaaa catgncnggt ttncnngngc tgnnnnnnnn gnnnctcgnn ngccantcnn gaancnnann nnngnatttc nnnnngnnnn nncgcnc
Expressed Protein Sequence
MAHHHHHHMN KPVTPKTPKL RSRAWFDNPD DVDMTALYLE RYMNYGLSQE ELQSGRPIIG IAQTGSDLSP CNRHHLELAK RVRDGVREAG GIVIEFPVHP IQETGKRPTA GLDRNLAYLG LVEVLYGYPL DGVVLTIGCD KTTPACLMAA ATVNIPAIAL SVGPMLNGWF RGERTGSGTI VWKARELLAK GEIDYQGFVK LVASSAPSTG YCNTMGTATT MNSLAEALGM QLPGSAAIPA PYRDRQEVAY LTGRRIVEMV HEDLKPSDIL TKEAFINAIR VNSAIGGSTN APIHLNALAR HIGVELTVDD WQKYGEEIPL LVNLQPAGEY LGEDYYHAGG VPAVVNQLMG QGLIHEDAIT VNGKTIGENC KNATIEDGNV IKTYDQPLKK HAGFRVLRGN LFSSAIMKLS VISDEFRNRY LSDAKDPNAF EGKAVVFDGP EDYHHRIDDP ALEIDEHTVL FMRGAGPIGY PGAAEVVNMR APDYLLKKGI TSLPCIGDGR QSGTSGSPSI LNASPEAAAG GGLAILKTGD RVRIDLGRGT ADILISDEEL AERRKALEAV GGYKYPESQT PWQEIQRAVI GQMETGAVLE NAVKYQDIAH TRGLPRDNH
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACA AGCCGGTTAC GCCAAAAACA CCCAAATTGC GGTCACGCGC ATGGTTCGAC AATCCCGACG ATGTCGATAT GACGGCGCTC TATCTTGAGC GTTACATGAA TTACGGGCTG AGCCAGGAAG AGCTGCAATC CGGCCGTCCC ATCATCGGTA TTGCGCAAAC CGGTTCTGAT CTTTCGCCCT GCAACCGGCA TCATCTGGAA CTGGCCAAGC GTGTGAGGGA TGGAGTTCGC GAGGCGGGTG GCATCGTCAT CGAATTTCCG GTTCATCCCA TTCAGGAAAC CGGCAAGCGC CCCACTGCGG GTCTCGACCG CAATCTGGCC TATCTTGGTC TGGTCGAAGT GCTTTACGGC TATCCGCTCG ATGGTGTGGT GCTGACCATC GGCTGCGACA AGACCACGCC CGCCTGCCTG ATGGCAGCGG CGACCGTCAA TATTCCGGCC ATCGCGCTGT CGGTCGGGCC GATGCTGAAC GGCTGGTTCC GGGGTGAGCG CACGGGTTCG GGCACCATCG TCTGGAAGGC GCGCGAACTG CTTGCCAAGG GCGAAATCGA CTATCAGGGC TTTGTGAAAC TGGTGGCTTC TTCGGCTCCA TCGACCGGCT ATTGCAACAC GATGGGCACG GCCACGACCA TGAATTCTCT GGCCGAAGCG CTGGGTATGC AGCTTCCGGG CTCGGCGGCT ATCCCCGCAC CTTATCGTGA CCGTCAGGAA GTCGCCTATC TGACGGGGCG GCGTATTGTC GAGATGGTTC ATGAAGACCT GAAACCATCG GATATCCTGA CGAAGGAAGC CTTTATCAAT GCGATCCGCG TCAATTCCGC CATTGGCGGC TCCACCAATG CGCCGATCCA TCTCAATGCG CTCGCGCGCC ATATCGGTGT GGAACTGACG GTGGATGACT GGCAGAAATA TGGCGAGGAA ATTCCGCTTC TGGTCAATCT CCAGCCTGCG GGCGAATATC TGGGCGAAGA CTATTATCAC GCAGGCGGTG TGCCAGCGGT GGTCAACCAG TTGATGGGGC AGGGCCTCAT CCATGAGGAC GCAATCACGG TCAATGGCAA GACCATCGGC GAAAATTGCA AGAATGCGAC CATCGAAGAC GGCAATGTCA TCAAGACCTA CGATCAGCCG CTCAAGAAAC ATGCCGGTTT CCGCGTGCTG CGCGGCAATC TGTTCTCGTC GGCCATCATG AAGCTCAGCG TGATTTCGGA CGAATTCCGC AATCGCTACC TGTCCGATGC CAAGGATCCC AATGCCTTTG AAGGCAAGGC GGTCGTGTTC GATGGGCCGG AGGATTATCA TCACCGTATT GATGATCCGG CGCTGGAAAT AGATGAACAT ACGGTGCTTT TCATGCGCGG TGCGGGACCG ATCGGCTATC CGGGTGCGGC GGAAGTCGTC AACATGCGCG CGCCGGATTA CCTTTTGAAG AAGGGCATCA CGTCGCTGCC CTGTATCGGC GACGGCCGCC AGTCGGGTAC GTCGGGTTCG CCGTCGATCC TCAATGCCTC GCCGGAAGCG GCGGCAGGCG GGGGGCTTGC CATTCTCAAG ACCGGCGACC GCGTGCGCAT CGATCTTGGC CGCGGGACGG CGGATATCCT GATTTCCGAT GAGGAATTGG CCGAACGCCG CAAGGCGCTG GAAGCGGTTG GCGGTTATAA ATATCCTGAA AGCCAGACGC CATGGCAGGA AATCCAGCGT GCTGTCATCG GCCAGATGGA AACCGGGGCC GTGCTGGAAA ACGCGGTCAA ATATCAGGAT ATCGCGCATA CGCGCGGGCT TCCGCGAGAT AACCATTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T