SSGCID: Target Page

BrabA.18158.d

Predicted regulator PutR for proline utilization, GntR family

CENTER ID:	BrabA.18158.d
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.18158.d.B1.GE37886	Full length( BrabA.18158.d )		1	228

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel86222_3274
BV-BRC:	fig\|359391.4.peg.3274
RefSeq:	YP_419115.1
UniProt:	Q2YJR9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAYEWRSQT RLLDEVAEAL RERIYAGDYA PGAILRQEHI ATEFGISRTP LREALRVLER DGLVIHLPGR GGRVASADLT RLIDAYAVRE VLDGVAARFA AERASDADIA KLRAHVAGQD KVVDPWDPKA YTQSNVDFHM AVMNTAGNAS LIAFVPLLRM TSQVFAPSFS LSVDRARDAI REHAAIVEAI AARDGEEAER LARAHIRATT ARLEAERPHR ENDNDARK

NT Sequence

TTGTCGGCTT ACGAGTGGCG CAGTCAGACC CGTCTGCTGG ATGAAGTTGC CGAGGCGCTT CGGGAGCGGA TTTATGCGGG CGATTATGCA CCGGGCGCAA TCTTGCGGCA GGAGCATATT GCGACGGAAT TTGGCATCAG CCGCACACCG CTGCGCGAGG CATTGCGTGT TCTGGAACGT GACGGACTGG TCATCCACCT GCCGGGCAGG GGGGGTCGTG TTGCCTCCGC CGATCTCACG CGCCTGATCG ATGCTTATGC TGTGCGTGAG GTGCTGGACG GCGTTGCGGC GCGTTTTGCC GCCGAACGGG CGAGCGATGC GGATATCGCA AAGCTGCGCG CGCATGTTGC GGGGCAGGAC AAGGTTGTCG ATCCGTGGGA TCCGAAAGCC TATACCCAAT CCAATGTCGA TTTTCACATG GCGGTGATGA ACACGGCGGG CAATGCCTCG CTGATTGCCT TCGTGCCACT TTTACGCATG ACATCGCAGG TTTTTGCGCC GTCCTTTTCA CTGTCGGTGG ACCGGGCGCG CGATGCAATC CGGGAACATG CAGCCATTGT CGAAGCCATT GCCGCCCGCG ATGGCGAGGA AGCCGAACGG CTGGCGCGGG CGCATATCCG CGCAACCACG GCAAGGCTGG AGGCGGAACG GCCTCATCGG GAGAATGACA ATGACGCAAG AAAA

Details for BrabA.18158.d.B1.GE37886

HARVESTED ON:	4/10/2014
SEQUENCED ON:	4/8/2014
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML SAYEWRSQTR LLDEVAEALR ERIYAGDYAP GAILRQEHIA TEFGISRTPL REALRVLERD GLVIHLPGRG GRVASADLTR LIDAYAVREV LDGVAARFAA ERASDADIAK LRAHVAGQDK VVDPWDPKAY TQSNVDFHMA VMNTAGNASL IAFVPLLRMT SQVFAPSFSL SVDRARDAIR EHAAIVEAIA ARDGEEAERL ARAHIRATTA RLEAERPHRE NDNDARK

Validated NT Sequence

tgttagcagc cggntcctat cttactcact tattttcttg cgtcattgtc attctcccga tgaggccgtt ccgcctccag ccttgccgtg gttgcgcgga tatgcgcccg cgccagccgt tcggcttcct cgccatcgcg ggcggcaatg gcttcgacaa tggctgcatg ttcccggatt gcatcgcgcg cccggtccac cgacagtgaa aaggacggcg caaaaacctg cgatgtcatg cgtaaaagtg gcacgaaggc aatcagcgag gcattgcccg ccgtgttcat caccgccatg tgaaaatcga cattggattg ggtataggct ttcggatccc acggatcgac aaccttgtcc tgccccgcaa catgcgcgcg cagctttgcg atatccgcat cgctcgcccg ttcggcggca aaacgcgccg caacgccgtc cagcacctca cgcacagcat aagcatcgat caggcgcgtg agatcggcgg aggcaacacg accccccctg cccggcaggt ggatgaccag tccgtcacgt tccagaacac gcaatgcctc gcgcagcggt gtgcggctga tgccaaattc cgtcgcaata tgctcctgcc gcaagattgc gcccggtgca taatcgcccg cataaatccg ctcccgaagc gcctcggcaa cttcatccag cagacgggtc tgactgcgcc actcgtaagc cgacaacata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHMS AYEWRSQTRL LDEVAEALRE RIYAGDYAPG AILRQEHIAT EFGISRTPLR EALRVLERDG LVIHLPGRGG RVASADLTRL IDAYAVREVL DGVAARFAAE RASDADIAKL RAHVAGQDKV VDPWDPKAYT QSNVDFHMAV MNTAGNASLI AFVPLLRMTS QVFAPSFSLS VDRARDAIRE HAAIVEAIAA RDGEEAERLA RAHIRATTAR LEAERPHREN DNDARK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgtcgg cttacgagtg gcgcagtcag acccgtctgc tggatgaagt tgccgaggcg cttcgggagc ggatttatgc gggcgattat gcaccgggcg caatcttgcg gcaggagcat attgcgacgg aatttggcat cagccgcaca ccgctgcgcg aggcattgcg tgttctggaa cgtgacggac tggtcatcca cctgccgggc agggggggtc gtgttgcctc cgccgatctc acgcgcctga tcgatgctta tgctgtgcgt gaggtgctgg acggcgttgc ggcgcgtttt gccgccgaac gggcgagcga tgcggatatc gcaaagctgc gcgcgcatgt tgcggggcag gacaaggttg tcgatccgtg ggatccgaaa gcctataccc aatccaatgt cgattttcac atggcggtga tgaacacggc gggcaatgcc tcgctgattg ccttcgtgcc acttttacgc atgacatcgc aggtttttgc gccgtccttt tcactgtcgg tggaccgggc gcgcgatgca atccgggaac atgcagccat tgtcgaagcc attgccgccc gcgatggcga ggaagccgaa cggctggcgc gggcgcatat ccgcgcaacc acggcaaggc tggaggcgga acggcctcat cgggagaatg acaatgacgc aagaaaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at