BrabA.17987.a

DNA polymerase III subunit beta (EC 2.7.7.7)

CENTER ID: BrabA.17987.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17987.a.B1.GE41242 full length 1 397
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.3
UniProt: Q2YPM4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLIDKGRLP LGCSRPGPSE RVRFKMRVTL ERSNLLKSLN HVHRVVERRN TIPILSNVLL QAEGASLAMK ATDLDLEVNE ATAAMVEQAG ATTVPAHLLY DIVRKLPDGA EVMLSTNPDG GSMSVISGKS SFRLQCLPQS DFPELTAGAF THSFRIEAQA LKRLIDRTQF AISTEETRYY LNGIFFHAIE SDGALKLRAV ATDGHRLARA ELEAPSGTEG MPGIIIPRKT VAELQKLVDV PDVVVTVELS DTKIRFTVGS VVLTSKLIDG TFPDYQRVIP SGNDKKLTID RQDFAASVDR VSTISSERGR AVKLSIADGQ LTLTVNNPDS GSATDELAAD YDGDPLDIGF NSKYLLDITG QLSGTDAVFM LADAGSPTLV RDTGDEDVLY VLMPMRV
NT Sequence
gtgcgcctta tcgacaaggg ccgtctgccc ctcggatgca gtcgccccgg tcctagcgag cgagtgcgtt tcaagatgcg tgttacccta gagcgatcga accttcttaa atctctcaac cacgtccatc gcgtcgtgga gcgccgcaac acgattccaa ttctgtcgaa tgtgcttttg caggccgaag gcgcaagcct cgccatgaag gcaaccgacc tggatctcga agtgaacgag gcgaccgccg ccatggtaga gcaggcgggc gccaccacgg tcccggcgca tcttctctat gatattgttc gcaagctgcc ggatggcgcg gaagtgatgc tgtccaccaa tcccgatggc ggctccatgt cggtcatttc gggcaagtct tctttccgcc tgcaatgcct gccgcaatcc gatttcccgg aactgaccgc tggcgccttc acccattcct tccgcatcga ggcgcaggct ctcaagcgcc tcatcgaccg cacgcagttt gcaatctcga cggaagaaac ccgttattac ctgaacggta tcttcttcca tgcgattgaa agcgatggcg cgttgaagct tcgggcggtt gcaaccgacg ggcaccgtct ggcgcgcgcc gaactggagg cgccctccgg cacggaaggc atgccgggca tcatcatccc gcgcaagacg gttgctgaat tgcagaagct ggtcgatgtg ccggatgtgg ttgtgaccgt tgagctttcc gataccaaga tccgcttcac cgtcggctcg gtggtgctca cctccaagct tatcgacggc actttcccgg attatcagcg cgtcattcca tcaggcaacg acaagaagct caccattgac cggcaggatt ttgctgcttc cgttgaccgc gtttcgacga tttcgagtga gcgtggccgc gcggtgaagc tttcgattgc cgatggacaa ttgacgctca ccgtcaacaa tcccgattcg ggcagtgcga ccgatgagct tgccgccgat tatgatggcg atccgctcga tatcggcttc aactccaaat atctgcttga tatcaccggc cagctttccg gtaccgatgc ggttttcatg ctggccgatg ccggctctcc gacgctggtg cgcgatacgg gcgatgaaga tgtgctctat gtcctcatgc cgatgcgcgt ctaa
Details for BrabA.17987.a.B1.GE41242
HARVESTED ON: data unavailable
SEQUENCED ON: 3/9/2017
EXPECTED MW: 44kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: None
TOTAL EXPRESSION LEVEL: Data Unavailable
SOLUBLE EXPRESSION LEVEL Data Unavailable
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV RLIDKGRLPL GCSRPGPSER VRFKMRVTLE RSNLLKSLNH VHRVVERRNT IPILSNVLLQ AEGASLAMKA TDLDLEVNEA TAAMVEQAGA TTVPAHLLYD IVRKLPDGAE VMLSTNPDGG SMSVISGKSS FRLQCLPQSD FPELTAGAFT HSFRIEAQAL KRLIDRTQFA ISTEETRYYL NGIFFHAIES DGALKLRAVA TDGHRLARAE LEAPSGTEGM PGIIIPRKTV AELQKLVDVP DVVVTVELSD TKIRFTVGSV VLTSKLIDGT FPDYQGVIPS GNDKKLTIDR QDFAASVDRV STISSERGRA VKLSIADGQL TLTVNNPDSG SATDELAADY DGDPLDIGFN SKYLLDITGQ LSGTDAVFML ADAGSPTLVR DTGDEDVL
Validated NT Sequence
atggctcacc accaccacca ccatatggtg cgccttatcg acaagggccg tctgcccctc ggatgcagtc gccccggtcc tagcgagcga gtgcgtttca agatgcgtgt taccctagag cgatcgaacc ttcttaaatc tctcaaccac gtccatcgcg tcgtggagcg ccgcaacacg attccaattc tgtcgaatgt gcttttgcag gccgaaggcg caagcctcgc catgaaggca accgacctgg atctcgaagt gaacgaggcg accgccgcca tggtagagca ggcgggcgcc accacggtcc cggcgcatct tctctatgat attgttcgca agctgccgga tggcgcggaa gtgatgctgt ccaccaatcc cgatggcggc tccatgtcgg tcatttcggg caagtcttct ttccgcctgc aatgcctgcc gcaatccgat ttcccggaac tgaccgctgg cgccttcacc cattccttcc gcatcgaggc gcaggctctc aagcgcctca tcgaccgcac gcagtttgca atctcgacgg aagaaacccg ttattacctg aacggtatct tcttccatgc gattgaaagc gatggcgcgt tgaagcttcg ggcggttgca accgacgggc accgtctggc gcgcgccgaa ctggaggcgc cctccggcac ggaaggcatg ccgggcatca tcatcccgcg caagacggtt gctgaattgc agaagctggt cgatgtgccg gatgtggttg tgaccgttga gctttccgat accaagatcc gcttcaccgt cggctcggtg gtgctcacct ccaagcttat cgacggcact ttcccggatt atcagggcgt cattccatca ggcaacgaca agaagctcac cattgaccgg caggattttg ctgcttccgt tgaccgcgtt tcgacgattt cgagtgagcg tggccgcgcg gtgaagcttt cgattgccga tggacaattg acgctcaccg tcaacaatcc cgattcgggc agtgcgaccg atgagcttgc cgccgattat gatggcgatc cgctcgatat cggcttcaac tccaaatatc tgcttgatat caccggccag ctttccggta ccgatgcggt tttcatgctg gccgatgccg gctctccgac gctggtgcgc gatacgggcg atgaagatgt gctct
Expected Protein Sequence
MAHHHHHHMR LIDKGRLPLG CSRPGPSERV RFKMRVTLER SNLLKSLNHV HRVVERRNTI PILSNVLLQA EGASLAMKAT DLDLEVNEAT AAMVEQAGAT TVPAHLLYDI VRKLPDGAEV MLSTNPDGGS MSVISGKSSF RLQCLPQSDF PELTAGAFTH SFRIEAQALK RLIDRTQFAI STEETRYYLN GIFFHAIESD GALKLRAVAT DGHRLARAEL EAPSGTEGMP GIIIPRKTVA ELQKLVDVPD VVVTVELSDT KIRFTVGSVV LTSKLIDGTF PDYQRVIPSG NDKKLTIDRQ DFAASVDRVS TISSERGRAV KLSIADGQLT LTVNNPDSGS ATDELAADYD GDPLDIGFNS KYLLDITGQL SGTDAVFMLA DAGSPTLVRD TGDEDVLYVL MPMRV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcgcc ttatcgacaa gggccgtctg cccctcggat gcagtcgccc cggtcctagc gagcgagtgc gtttcaagat gcgtgttacc ctagagcgat cgaaccttct taaatctctc aaccacgtcc atcgcgtcgt ggagcgccgc aacacgattc caattctgtc gaatgtgctt ttgcaggccg aaggcgcaag cctcgccatg aaggcaaccg acctggatct cgaagtgaac gaggcgaccg ccgccatggt agagcaggcg ggcgccacca cggtcccggc gcatcttctc tatgatattg ttcgcaagct gccggatggc gcggaagtga tgctgtccac caatcccgat ggcggctcca tgtcggtcat ttcgggcaag tcttctttcc gcctgcaatg cctgccgcaa tccgatttcc cggaactgac cgctggcgcc ttcacccatt ccttccgcat cgaggcgcag gctctcaagc gcctcatcga ccgcacgcag tttgcaatct cgacggaaga aacccgttat tacctgaacg gtatcttctt ccatgcgatt gaaagcgatg gcgcgttgaa gcttcgggcg gttgcaaccg acgggcaccg tctggcgcgc gccgaactgg aggcgccctc cggcacggaa ggcatgccgg gcatcatcat cccgcgcaag acggttgctg aattgcagaa gctggtcgat gtgccggatg tggttgtgac cgttgagctt tccgatacca agatccgctt caccgtcggc tcggtggtgc tcacctccaa gcttatcgac ggcactttcc cggattatca gcgcgtcatt ccatcaggca acgacaagaa gctcaccatt gaccggcagg attttgctgc ttccgttgac cgcgtttcga cgatttcgag tgagcgtggc cgcgcggtga agctttcgat tgccgatgga caattgacgc tcaccgtcaa caatcccgat tcgggcagtg cgaccgatga gcttgccgcc gattatgatg gcgatccgct cgatatcggc ttcaactcca aatatctgct tgatatcacc ggccagcttt ccggtaccga tgcggttttc atgctggccg atgccggctc tccgacgctg gtgcgcgata cgggcgatga agatgtgctc tatgtcctca tgccgatgcg cgtctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat