BrabA.17757.a

ABC transporter substrate-binding protein KO: K02051 sulfonate/nitrate transport system substrate-binding protein

CENTER ID:	BrabA.17757.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	native diffraction-data
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17757.a.A2.GE32530	Mature protein( BrabA.17757.a )		22	322

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17757.a.A2.PS01131	Mature protein( BrabA.17757.a )		22	322

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_133123
PATRIC ID:	fig\|359391.4.peg.224
RefSeq:	YP_413696.1
UniProt:	Q2YP60

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKMAFAALF GAVLSFASSA EANDKLKLIL DWYVNPDHGP IIIANKLGYF RQAGLDVDIV APADASVPTK MVAAGQADLA VSYQQQVHLQ VHEGLPVIRV GTLIDSPLNC LMTRDDGSVN AIADLKGKKI GYSVAGVEET LLGTILGQHG VKLSDVELIN VNFSLAPALM SKQVDAVIGA YRNVELNQMA VEGVAGKCFF VEEEGVPVYD ELVYVANSDK LDAAERQKIS RFLAATEKAA QYIVNHPEKS YEIFSSYSTE LKDELNQRAW KDTVARFSRA PASLDHGRWS RYESYLKEHG LVPSILPVEK LAIDVNAEGS KT

NT Sequence

ATGAAGAAAA TGGCATTTGC CGCGCTGTTC GGCGCGGTGC TCAGCTTCGC CTCGTCGGCG GAAGCCAATG ACAAGCTGAA GCTCATTCTC GACTGGTATG TGAACCCCGA CCATGGGCCG ATTATCATTG CCAACAAGCT CGGCTATTTC AGGCAAGCGG GGCTGGACGT GGATATCGTC GCGCCTGCCG ATGCTTCCGT GCCCACGAAA ATGGTTGCTG CCGGCCAGGC CGATCTTGCC GTTTCCTATC AGCAGCAGGT GCATCTGCAA GTACATGAGG GCCTGCCGGT CATCCGCGTC GGCACGCTGA TCGATTCGCC GCTCAATTGC CTGATGACCC GCGATGACGG ATCGGTGAAT GCAATCGCCG ACCTGAAAGG CAAAAAGATC GGCTATTCGG TTGCAGGCGT TGAGGAAACC CTGCTCGGCA CCATTCTCGG CCAGCATGGC GTCAAGCTTT CCGATGTCGA ACTCATCAAT GTCAATTTCT CGCTGGCGCC AGCGCTCATG TCGAAACAGG TGGATGCGGT GATAGGCGCC TATCGCAATG TGGAACTGAA CCAGATGGCC GTCGAGGGCG TGGCGGGCAA GTGCTTCTTC GTGGAGGAGG AAGGCGTGCC GGTCTATGAC GAGCTGGTCT ATGTCGCAAA TAGCGACAAG CTTGATGCGG CAGAGAGGCA AAAGATTTCC CGCTTCCTCG CCGCCACCGA AAAGGCCGCG CAATATATCG TCAATCACCC GGAGAAGAGT TACGAGATCT TTTCCTCCTA CAGCACGGAA CTGAAGGACG AATTGAACCA GCGCGCGTGG AAAGACACGG TTGCCCGCTT CTCGCGCGCA CCAGCAAGCC TCGATCATGG CCGCTGGAGC CGCTATGAAT CCTATCTGAA GGAGCATGGC CTCGTGCCCT CCATCCTGCC GGTCGAAAAA TTGGCCATCG ACGTCAATGC GGAAGGGAGC AAGACA

Details for BrabA.17757.a.A2.GE32530

HARVESTED ON:	5/17/2011
SEQUENCED ON:	5/23/2011
EXPECTED MW:	35kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	18
PERCENT COVERAGE:	67

Validated AA Sequence

MIEAXWCARE AGNRVXXRAL XQXXLQFRAV GGKDLVTLLR VIDDILRXLF GGGEEAGNLL PLCRIKLVAI CDIDQLVIDR HAFLLHEEAL ARHALDGHLV QFHIAIGAYH RIHLFRHERW RQREIDIDEF DIGKLDAMLA ENGAEQGFLN ACNRIADLFA FQVGDCIHRS VIAGHXAIER RIDQRADXDX RXALMYLQMH LLLIGNGKIX LAGSNHFRG

Validated NT Sequence

ccatgatcga ggcttgntgg tgcgcgcgag aagcnggcaa ccgtgtcttn nnacgcgcgc tgnttcaatt nntncttcag ttccgtgcng taggaggaaa agatctngta actcttctcc gggtgattga cgatatattg cgcgnccttt tcggtggcgg cgaggaagcg ggaaatcttt tgcctctctg ccgcatcaag ctngtcgcta tttgcgacat agaccagctc gtcatagacc ggcacgcctt cctcctccac gaagaagcac ttgcccgcca cgccctcgac ggccatctgg ttcagttcca cattgcgata ggcgcctatc accgcatcca cctgtttcga catgagcgct ggcgccagcg agaaattgac attgatgagt tcgacatcgg aaagcttgac gccatgctgg ccgagaatgg tgccgagcag ggtttcctca acgcctgcaa ccgaatagcc gatctttttg cctttcaggt cggcgattgc attcaccgat ccgtcatcgc gggtcatcan gcaattgagc ggcgaatcga tcagcgtgcc gacncggatg ancggcangc cctcatgtac ttgcagatgc acctgctgct gataggaaac ggcaagatcn gnctggccgg cagcaaccat tttcgtgggc a

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMANDKLKLI LDWYVNPDHG PIIIANKLGY FRQAGLDVDI VAPADASVPT KMVAAGQADL AVSYQQQVHL QVHEGLPVIR VGTLIDSPLN CLMTRDDGSV NAIADLKGKK IGYSVAGVEE TLLGTILGQH GVKLSDVELI NVNFSLAPAL MSKQVDAVIG AYRNVELNQM AVEGVAGKCF FVEEEGVPVY DELVYVANSD KLDAAERQKI SRFLAATEKA AQYIVNHPEK SYEIFSSYST ELKDELNQRA WKDTVARFSR APASLDHGRW SRYESYLKEH GLVPSILPVE KLAIDVNAEG SKT

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccaatgac aagctgaagc tcattctcga ctggtatgtg aaccccgacc atgggccgat tatcattgcc aacaagctcg gctatttcag gcaagcgggg ctggacgtgg atatcgtcgc gcctgccgat gcttccgtgc ccacgaaaat ggttgctgcc ggccaggccg atcttgccgt ttcctatcag cagcaggtgc atctgcaagt acatgagggc ctgccggtca tccgcgtcgg cacgctgatc gattcgccgc tcaattgcct gatgacccgc gatgacggat cggtgaatgc aatcgccgac ctgaaaggca aaaagatcgg ctattcggtt gcaggcgttg aggaaaccct gctcggcacc attctcggcc agcatggcgt caagctttcc gatgtcgaac tcatcaatgt caatttctcg ctggcgccag cgctcatgtc gaaacaggtg gatgcggtga taggcgccta tcgcaatgtg gaactgaacc agatggccgt cgagggcgtg gcgggcaagt gcttcttcgt ggaggaggaa ggcgtgccgg tctatgacga gctggtctat gtcgcaaata gcgacaagct tgatgcggca gagaggcaaa agatttcccg cttcctcgcc gccaccgaaa aggccgcgca atatatcgtc aatcacccgg agaagagtta cgagatcttt tcctcctaca gcacggaact gaaggacgaa ttgaaccagc gcgcgtggaa agacacggtt gcccgcttct cgcgcgcacc agcaagcctc gatcatggcc gctggagccg ctatgaatcc tatctgaagg agcatggcct cgtgccctcc atcctgccgg tcgaaaaatt ggccatcgac gtcaatgcgg aagggagcaa gacataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for BrabA.17757.a.A2.PS01131

PURIFICATION DATe:	6/22/2011
CONCENTRATION:	63.53mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	18
PERCENT COVERAGE:	67

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCAATGAC AAGCTGAAGC TCATTCTCGA CTGGTATGTG AACCCCGACC ATGGGCCGAT TATCATTGCC AACAAGCTCG GCTATTTCAG GCAAGCGGGG CTGGACGTGG ATATCGTCGC GCCTGCCGAT GCTTCCGTGC CCACGAAAAT GGTTGCTGCC GGCCAGGCCG ATCTTGCCGT TTCCTATCAG CAGCAGGTGC ATCTGCAAGT ACATGAGGGC CTGCCGGTCA TCCGCGTCGG CACGCTGATC GATTCGCCGC TCAATTGCCT GATGACCCGC GATGACGGAT CGGTGAATGC AATCGCCGAC CTGAAAGGCA AAAAGATCGG CTATTCGGTT GCAGGCGTTG AGGAAACCCT GCTCGGCACC ATTCTCGGCC AGCATGGCGT CAAGCTTTCC GATGTCGAAC TCATCAATGT CAATTTCTCG CTGGCGCCAG CGCTCATGTC GAAACAGGTG GATGCGGTGA TAGGCGCCTA TCGCAATGTG GAACTGAACC AGATGGCCGT CGAGGGCGTG GCGGGCAAGT GCTTCTTCGT GGAGGAGGAA GGCGTGCCGG TCTATGACGA GCTGGTCTAT GTCGCAAATA GCGACAAGCT TGATGCGGCA GAGAGGCAAA AGATTTCCCG CTTCCTCGCC GCCACCGAAA AGGCCGCGCA ATATATCGTC AATCACCCGG AGAAGAGTTA CGAGATCTTT TCCTCCTACA GCACGGAACT GAAGGACGAA TTGAACCAGC GCGCGTGGAA AGACACGGTT GCCCGCTTCT CGCGCGCACC AGCAAGCCTC GATCATGGCC GCTGGAGCCG CTATGAATCC TATCTGAAGG AGCATGGCCT CGTGCCCTCC ATCCTGCCGG TCGAAAAATT GGCCATCGAC GTCAATGCGG AAGGGAGCAA GACATAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA