BrabA.17745.a

Glycosyl transferase, group 1

CENTER ID: BrabA.17745.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17745.a.A1.GE31735 Full length( BrabA.17745.a ) 1 369
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.591
RefSeq: YP_414015.1
UniProt: Q2YMQ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRVLHFFKTY WPDTFGGIER TIHAIAKGVA EHGIASDVLS LSAKPEENTR NFDGHMAYKA KLDLEFASTG LSRDVFSRFR ELSSQADVIH YHFPWPMSDI VQIGVRPDKP TIVTYHSDIV KQKLLLQFYR PLMNRFLGSV DRIVATSPNY LATSDVLQRF SDKTTVIPLG LDESDYSAAD ATTKARWRER FPRPFFLFVG VLRYYKGLRT LLAAARTSDI DIVIVGDGPM KPQLIAYANE HNLSHVHFAG ALPDTDKTAL LELSAGLIFP SHLRSEAFGL SLVEASMFGK PMISCEIGTG TSYVNLNGQT GIVVPPQNPE ALSAAMRSIA HDTEQAKVFG MNARARYIEH FTADKMALEY AKNYTSLMQ
NT Sequence
ATGCGAGTTC TTCATTTTTT TAAGACCTAT TGGCCAGATA CATTTGGCGG CATTGAGCGA ACCATTCATG CGATCGCAAA AGGTGTGGCC GAACACGGTA TCGCGTCGGA CGTGTTATCC TTGAGTGCAA AGCCGGAAGA AAATACCCGA AATTTTGATG GTCACATGGC ATATAAGGCC AAGCTCGACC TTGAATTTGC ATCAACAGGA TTGTCGCGCG ATGTGTTTTC CCGGTTTCGG GAGTTGAGCA GCCAAGCTGA TGTCATTCAT TATCATTTCC CCTGGCCCAT GAGCGATATC GTGCAGATCG GTGTGCGCCC GGATAAGCCG ACTATCGTGA CCTATCATTC GGATATCGTT AAGCAGAAAC TACTTCTTCA GTTCTATCGC CCCTTGATGA ACCGCTTTCT CGGCAGCGTT GACCGGATCG TTGCCACCTC TCCGAATTAT CTTGCGACAA GCGATGTCCT GCAGCGGTTC AGCGATAAAA CGACAGTCAT TCCACTTGGC TTGGACGAGT CTGATTATTC TGCTGCCGAC GCCACAACCA AGGCGCGCTG GCGCGAGCGG TTTCCGCGCC CTTTCTTTCT CTTCGTCGGC GTGCTGCGTT ATTATAAAGG GCTAAGAACG CTACTGGCCG CAGCAAGAAC TTCCGATATT GATATTGTCA TTGTCGGGGA TGGGCCTATG AAACCTCAGC TCATCGCGTA TGCGAATGAG CATAATCTGT CGCACGTTCA TTTCGCCGGC GCGTTGCCGG ATACGGACAA AACTGCGCTT CTGGAGTTGA GTGCTGGGCT GATTTTTCCG TCGCATCTGC GCTCGGAAGC GTTTGGGCTA TCGCTGGTGG AGGCCTCTAT GTTTGGAAAG CCGATGATCT CGTGTGAGAT CGGTACCGGC ACGAGTTATG TCAATCTGAA TGGGCAAACG GGAATCGTGG TGCCGCCTCA GAACCCGGAA GCGCTCTCAG CTGCAATGCG AAGCATTGCA CACGATACGG AGCAGGCCAA AGTTTTTGGA ATGAACGCAC GGGCGCGATA TATAGAGCAT TTCACTGCTG ATAAAATGGC GTTAGAATAT GCGAAAAATT ATACGTCGCT GATGCAG
Details for BrabA.17745.a.A1.GE31735
HARVESTED ON: 2/23/2011
SEQUENCED ON: 3/1/2011
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 79
Validated AA Sequence
MRVLHFFKTY WPDTFGGIER TIHAIAKGVA EHGIASDVLS LSAKPEENTR NFDGHMAYKA KLDLEFASTG LSRDVFSRFR ELSSQADVIX YXFPWPMXDI VXIGVRPDKP TIVTYHSDIV KQKLLLQFYR PXMNRFLGSV DRIVATSXNY XATSDVXQRF SDKTTVIPLG LDESDYSAAD ATTKARWRER FPRPFFLXVG VLRYYKGLRT LLAAARTSDI DIVIVGDGPM KPQLIAYANE HNLSHVHFAG ALPDTDKTAL LELSAGLIFP SHLRSEAFGL SLVEASMFGK PMISCEIGTG TSYVNLNGQ
Validated NT Sequence
gtttgcccat tcagattgac ataactcgtg ccggtaccga tctcacacga gatcatcggc tttccaaaca tagaggcctc caccagcgat agcccaaacg cttccgagcg cagatgcgac ggaaaaatca gcccagcact caactccaga agcgcagttt tgtccgtatc cggcaacgcg ccggcgaaat gaacgtgcga cagattatgc tcattcgcat acgcgatgag ctgaggtttc ataggcccat ccccgacaat gacaatatca atatcggaag ttcttgctgc ggccagtagc gttcttagcc ctttataata acgcagcacg ccgacnaaga gaaagaaagg gcgcggaaan cgctcgcgcc agcgcgcctt ggttgtggcg tcggcagcag aataatcaga ctcgtccaag ccaagtggaa tgactgtcgt tttatcgctg aaccgctgcn ngacatcgct tgtcgcanga taattcgnag aggtggcaac gatccggtca acgctgccna gaaagcggtt catcangggg cgatagaact gaagaagtag tttctgctta acgatatccg aatgataggt cacgatagtc ggcttatccg ggcgcacncc gatntgcacg atatcgntca tgggccaggg gaaannatan tgaatgacat cagcttggct gctcaactcc cgaaaccggg aaaacacatc gcgcgacaat cctgttgatg caaattcaag gtcgagcttg gccttatatg ccatgtgacc atcaaaattt cgggtatttt cttccggctt tgcactcaag gataacacgt ccgacgcgat accgtgttcg gccacacctt ttgcgatcgc atgaatggtt cgctcaatgc cgccaaatgt atctggccaa taggtcttaa aaaaatgaag aactcgcatc gaaccaggac cctgggtctg agcttccagg gta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRVLHFFKT YWPDTFGGIE RTIHAIAKGV AEHGIASDVL SLSAKPEENT RNFDGHMAYK AKLDLEFAST GLSRDVFSRF RELSSQADVI HYHFPWPMSD IVQIGVRPDK PTIVTYHSDI VKQKLLLQFY RPLMNRFLGS VDRIVATSPN YLATSDVLQR FSDKTTVIPL GLDESDYSAA DATTKARWRE RFPRPFFLFV GVLRYYKGLR TLLAAARTSD IDIVIVGDGP MKPQLIAYAN EHNLSHVHFA GALPDTDKTA LLELSAGLIF PSHLRSEAFG LSLVEASMFG KPMISCEIGT GTSYVNLNGQ TGIVVPPQNP EALSAAMRSI AHDTEQAKVF GMNARARYIE HFTADKMALE YAKNYTSLMQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgagttctt cattttttta agacctattg gccagataca tttggcggca ttgagcgaac cattcatgcg atcgcaaaag gtgtggccga acacggtatc gcgtcggacg tgttatcctt gagtgcaaag ccggaagaaa atacccgaaa ttttgatggt cacatggcat ataaggccaa gctcgacctt gaatttgcat caacaggatt gtcgcgcgat gtgttttccc ggtttcggga gttgagcagc caagctgatg tcattcatta tcatttcccc tggcccatga gcgatatcgt gcagatcggt gtgcgcccgg ataagccgac tatcgtgacc tatcattcgg atatcgttaa gcagaaacta cttcttcagt tctatcgccc cttgatgaac cgctttctcg gcagcgttga ccggatcgtt gccacctctc cgaattatct tgcgacaagc gatgtcctgc agcggttcag cgataaaacg acagtcattc cacttggctt ggacgagtct gattattctg ctgccgacgc cacaaccaag gcgcgctggc gcgagcggtt tccgcgccct ttctttctct tcgtcggcgt gctgcgttat tataaagggc taagaacgct actggccgca gcaagaactt ccgatattga tattgtcatt gtcggggatg ggcctatgaa acctcagctc atcgcgtatg cgaatgagca taatctgtcg cacgttcatt tcgccggcgc gttgccggat acggacaaaa ctgcgcttct ggagttgagt gctgggctga tttttccgtc gcatctgcgc tcggaagcgt ttgggctatc gctggtggag gcctctatgt ttggaaagcc gatgatctcg tgtgagatcg gtaccggcac gagttatgtc aatctgaatg ggcaaacggg aatcgtggtg ccgcctcaga acccggaagc gctctcagct gcaatgcgaa gcattgcaca cgatacggag caggccaaag tttttggaat gaacgcacgg gcgcgatata tagagcattt cactgctgat aaaatggcgt tagaatatgc gaaaaattat acgtcgctga tgcagtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa