BrabA.17719.a

Periplasmic binding protein

CENTER ID: BrabA.17719.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17719.a.A2.GE32527 Mature protein( BrabA.17719.a ) 33 315
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17719.a.A2.PS01127 Mature protein( BrabA.17719.a ) 33 315

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.2862
RefSeq: YP_418750.1
UniProt: Q2YKT4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKGSNIMLTF RQKITRLCGV AAMALALSSA AYAADITVKH AQGETAVPAS PEKVVVFDLA SLDNLDRLGV KVTGVPSGVP FPEYLKKYQG DDYAKVGTLF EPDYEAVNAA EPDLIIVGGR SAAKYAALKK IAPTVDLTVD ARNFIADTEA NIEKLGQIFG KEAEAKAEAE KLNKELAALH EKAAGKGTGL LILTSGGKIS AYGPGSRFGV LHDSFGVKPA APNLSIGNHG QPISSEFILE TNPDWLFVID RDAAIGREGN SAKQLLDNEL VRQTNAWKKD QVVYLDAQNW YLVGGGFSAL HNTIRQLSEA FDKSK
NT Sequence
ATGAAAGGAT CGAATATCAT GCTTACTTTC CGCCAAAAAA TTACCCGGCT TTGCGGTGTT GCCGCGATGG CGCTTGCCCT GTCGTCTGCT GCTTATGCCG CCGATATCAC GGTAAAACAT GCGCAGGGCG AAACAGCGGT TCCGGCCTCG CCGGAAAAGG TCGTGGTCTT CGATCTGGCA AGCCTCGACA ATCTTGACCG TCTCGGCGTC AAGGTGACGG GTGTTCCAAG CGGCGTGCCT TTCCCTGAAT ATCTGAAGAA ATATCAGGGC GACGACTATG CAAAGGTCGG CACGCTGTTC GAGCCGGACT ATGAAGCAGT CAATGCCGCA GAACCGGACC TCATCATTGT CGGTGGCCGT TCCGCCGCCA AATATGCCGC GTTGAAGAAG ATCGCACCGA CCGTTGACCT CACCGTTGAT GCCAGGAATT TCATTGCCGA CACGGAAGCC AATATCGAAA AGCTTGGCCA GATTTTCGGC AAGGAAGCGG AAGCCAAGGC TGAGGCCGAA AAGCTCAACA AGGAACTTGC CGCACTGCAC GAAAAGGCCG CAGGCAAAGG CACCGGCCTT CTTATCCTCA CTTCCGGTGG CAAAATCAGT GCCTACGGCC CCGGCTCGCG TTTCGGCGTC CTGCATGACA GCTTCGGTGT GAAGCCTGCG GCCCCCAATC TTTCCATCGG CAATCACGGC CAGCCAATCA GCTCGGAATT CATTCTGGAA ACCAATCCGG ATTGGCTGTT CGTGATCGAC CGCGACGCTG CCATCGGTCG CGAAGGCAAT TCCGCCAAGC AATTGCTCGA TAATGAACTC GTCCGTCAGA CGAATGCATG GAAGAAGGAT CAGGTGGTCT ATCTGGACGC CCAGAACTGG TATCTGGTCG GCGGCGGCTT CAGTGCACTG CACAACACCA TCAGACAGCT TTCCGAAGCT TTCGACAAGA GCAAG
Details for BrabA.17719.a.A2.GE32527
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 32kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAADITVKH AQGETAVPAS PEKVVVFDLA SLDNLDRLGV KVTGVPSGVP FPEYLKKYQG DDYAKVGTLF EPDYEAVNAA EPDLIIVGGR SAAKYAALKK IAPTVDLTVD ARNFIADTEA NIEKLGQIFG KEAEAKAEAE KLNKELAALH EKAAGKGTGL LILTSGGKIS AYGPGSRFGV LHDSFGVKPA APNLSIGNHG QPISSEFILE TNPDWLFVID RDAAIGREGN SAKQLLDNEL VRQTNAWKKD QVVYLDAQNW YLVGGGFSAL HNTIRQLSEA FDKSK
Validated NT Sequence
ttattacttg ctcttgtcga aagcttcgga aagctgtctg atggtgttgt gcagtgcact gaagccgccg ccgaccagat accagttctg ggcgtccaga tagaccacct gatccttctt ccatgcattc gtctgacgga cgagttcatt atcgagcaat tgcttggcgg aattgccttc gcgaccgatg gcagcgtcgc ggtcgatcac gaacagccaa tccggattgg tttccagaat gaattccgag ctgattggct ggccgtgatt gccgatggaa agattggggg ccgcaggctt cacaccgaag ctgtcatgca ggacgccgaa acgcgagccg gggccgtagg cactgatttt gccaccggaa gtgaggataa gaaggccggt gcctttgcct gcggcctttt cgtgcagtgc ggcaagttcc ttgttgagct tttcggcctc agccttggct tccgcttcct tgccgaaaat ctggccaagc ttttcgatat tggcttccgt gtcggcaatg aaattcctgg catcaacggt gaggtcaacg gtcggtgcga tcttcttcaa cgcggcatat ttggcggcgg aacggccacc gacaatgatg aggtccggtt ctgcggcatt gactgcttca tagtccggct cgaacagcgt gccgaccttt gcatagtcgt cgccctgata tttcttcaga tattcaggga aaggcacgcc gcttggaaca cccgtcacct tgacgccgag acggtcaaga ttgtcgaggc ttgccagatc gaagaccacg accttttccg gcgaggccgg aaccgctgtt tcgccctgcg catgttttac cgtgatatcg gcggccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAADITVKH AQGETAVPAS PEKVVVFDLA SLDNLDRLGV KVTGVPSGVP FPEYLKKYQG DDYAKVGTLF EPDYEAVNAA EPDLIIVGGR SAAKYAALKK IAPTVDLTVD ARNFIADTEA NIEKLGQIFG KEAEAKAEAE KLNKELAALH EKAAGKGTGL LILTSGGKIS AYGPGSRFGV LHDSFGVKPA APNLSIGNHG QPISSEFILE TNPDWLFVID RDAAIGREGN SAKQLLDNEL VRQTNAWKKD QVVYLDAQNW YLVGGGFSAL HNTIRQLSEA FDKSK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgccgat atcacggtaa aacatgcgca gggcgaaaca gcggttccgg cctcgccgga aaaggtcgtg gtcttcgatc tggcaagcct cgacaatctt gaccgtctcg gcgtcaaggt gacgggtgtt ccaagcggcg tgcctttccc tgaatatctg aagaaatatc agggcgacga ctatgcaaag gtcggcacgc tgttcgagcc ggactatgaa gcagtcaatg ccgcagaacc ggacctcatc attgtcggtg gccgttccgc cgccaaatat gccgcgttga agaagatcgc accgaccgtt gacctcaccg ttgatgccag gaatttcatt gccgacacgg aagccaatat cgaaaagctt ggccagattt tcggcaagga agcggaagcc aaggctgagg ccgaaaagct caacaaggaa cttgccgcac tgcacgaaaa ggccgcaggc aaaggcaccg gccttcttat cctcacttcc ggtggcaaaa tcagtgccta cggccccggc tcgcgtttcg gcgtcctgca tgacagcttc ggtgtgaagc ctgcggcccc caatctttcc atcggcaatc acggccagcc aatcagctcg gaattcattc tggaaaccaa tccggattgg ctgttcgtga tcgaccgcga cgctgccatc ggtcgcgaag gcaattccgc caagcaattg ctcgataatg aactcgtccg tcagacgaat gcatggaaga aggatcaggt ggtctatctg gacgcccaga actggtatct ggtcggcggc ggcttcagtg cactgcacaa caccatcaga cagctttccg aagctttcga caagagcaag taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BrabA.17719.a.A2.PS01127
PURIFICATION DATe: 6/24/2011
CONCENTRATION: 46.55mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAADITVKH AQGETAVPAS PEKVVVFDLA SLDNLDRLGV KVTGVPSGVP FPEYLKKYQG DDYAKVGTLF EPDYEAVNAA EPDLIIVGGR SAAKYAALKK IAPTVDLTVD ARNFIADTEA NIEKLGQIFG KEAEAKAEAE KLNKELAALH EKAAGKGTGL LILTSGGKIS AYGPGSRFGV LHDSFGVKPA APNLSIGNHG QPISSEFILE TNPDWLFVID RDAAIGREGN SAKQLLDNEL VRQTNAWKKD QVVYLDAQNW YLVGGGFSAL HNTIRQLSEA FDKSK
Validated NT Sequence
ttattacttg ctcttgtcga aagcttcgga aagctgtctg atggtgttgt gcagtgcact gaagccgccg ccgaccagat accagttctg ggcgtccaga tagaccacct gatccttctt ccatgcattc gtctgacgga cgagttcatt atcgagcaat tgcttggcgg aattgccttc gcgaccgatg gcagcgtcgc ggtcgatcac gaacagccaa tccggattgg tttccagaat gaattccgag ctgattggct ggccgtgatt gccgatggaa agattggggg ccgcaggctt cacaccgaag ctgtcatgca ggacgccgaa acgcgagccg gggccgtagg cactgatttt gccaccggaa gtgaggataa gaaggccggt gcctttgcct gcggcctttt cgtgcagtgc ggcaagttcc ttgttgagct tttcggcctc agccttggct tccgcttcct tgccgaaaat ctggccaagc ttttcgatat tggcttccgt gtcggcaatg aaattcctgg catcaacggt gaggtcaacg gtcggtgcga tcttcttcaa cgcggcatat ttggcggcgg aacggccacc gacaatgatg aggtccggtt ctgcggcatt gactgcttca tagtccggct cgaacagcgt gccgaccttt gcatagtcgt cgccctgata tttcttcaga tattcaggga aaggcacgcc gcttggaaca cccgtcacct tgacgccgag acggtcaaga ttgtcgaggc ttgccagatc gaagaccacg accttttccg gcgaggccgg aaccgctgtt tcgccctgcg catgttttac cgtgatatcg gcggccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAADITVKH AQGETAVPAS PEKVVVFDLA SLDNLDRLGV KVTGVPSGVP FPEYLKKYQG DDYAKVGTLF EPDYEAVNAA EPDLIIVGGR SAAKYAALKK IAPTVDLTVD ARNFIADTEA NIEKLGQIFG KEAEAKAEAE KLNKELAALH EKAAGKGTGL LILTSGGKIS AYGPGSRFGV LHDSFGVKPA APNLSIGNHG QPISSEFILE TNPDWLFVID RDAAIGREGN SAKQLLDNEL VRQTNAWKKD QVVYLDAQNW YLVGGGFSAL HNTIRQLSEA FDKSK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGCCGAT ATCACGGTAA AACATGCGCA GGGCGAAACA GCGGTTCCGG CCTCGCCGGA AAAGGTCGTG GTCTTCGATC TGGCAAGCCT CGACAATCTT GACCGTCTCG GCGTCAAGGT GACGGGTGTT CCAAGCGGCG TGCCTTTCCC TGAATATCTG AAGAAATATC AGGGCGACGA CTATGCAAAG GTCGGCACGC TGTTCGAGCC GGACTATGAA GCAGTCAATG CCGCAGAACC GGACCTCATC ATTGTCGGTG GCCGTTCCGC CGCCAAATAT GCCGCGTTGA AGAAGATCGC ACCGACCGTT GACCTCACCG TTGATGCCAG GAATTTCATT GCCGACACGG AAGCCAATAT CGAAAAGCTT GGCCAGATTT TCGGCAAGGA AGCGGAAGCC AAGGCTGAGG CCGAAAAGCT CAACAAGGAA CTTGCCGCAC TGCACGAAAA GGCCGCAGGC AAAGGCACCG GCCTTCTTAT CCTCACTTCC GGTGGCAAAA TCAGTGCCTA CGGCCCCGGC TCGCGTTTCG GCGTCCTGCA TGACAGCTTC GGTGTGAAGC CTGCGGCCCC CAATCTTTCC ATCGGCAATC ACGGCCAGCC AATCAGCTCG GAATTCATTC TGGAAACCAA TCCGGATTGG CTGTTCGTGA TCGACCGCGA CGCTGCCATC GGTCGCGAAG GCAATTCCGC CAAGCAATTG CTCGATAATG AACTCGTCCG TCAGACGAAT GCATGGAAGA AGGATCAGGT GGTCTATCTG GACGCCCAGA ACTGGTATCT GGTCGGCGGC GGCTTCAGTG CACTGCACAA CACCATCAGA CAGCTTTCCG AAGCTTTCGA CAAGAGCAAG TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA