SSGCID: Target Page

BrabA.17716.b

Urease alpha subunit (EC 3.5.1.5)

CENTER ID:	BrabA.17716.b
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17716.b.B1.GE37966	Full length( BrabA.17716.b )		1	570

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel86222_0320
BV-BRC:	fig\|359391.4.peg.320
RefSeq:	YP_413771.1
UniProt:	Q2YPD5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPARISRATY AQMFGPTVGD KVRLADTDLI IEVERDLTTY GEEVKFGGGK VIRDGMGQSQ LSRAEGAMDT VITNALILDH SGIYKADIGL LDGRIALIGK AGNPDTQPGI SIIIGPGTEI IAGEGKIVTA GGIDTHVHFI SPQQVDEALN AGITCMVGGG TGPAHGTLAT TCTPGPWHIA RLIQSFDGLP MNIGVFGKGN ASLPGALEEM VRAGACGLKL HEDWGCTPAA IDNCLSVADH FDVQVAIHTD TLNEGGFVED TLNAFKGRTI HSFHTEGAGG GHAPDIIRVC QYPNVLPAST NPTRPYTVNT IAEHLDMLMV CHHLSPAIPE DIAFAESRIR KETIAAEDIL HDMGAFSIIS SDSQAMGRVG EMIIRCWQTA DKMKKQRGSL PDDRPGNDNY RARRYIAKYT INPAIAHGMA HEIGSVEVGK RADLVLWNPA FFGVKPDMVL LGGWIATAPM GDANGSIPTP QPMHTRPMFG SFGKALTNTS ITFVSQAAMD EGLREKIGVD KQLVAVVNTR GGIGKHSMIL NNAMPQMEVD PETYEVRADG ELLTCEPVDV VPMAQRYFLF

NT Sequence

ATGCCAGCAA GAATTAGCCG CGCCACCTAT GCGCAAATGT TCGGCCCCAC CGTTGGCGAC AAGGTGCGCC TTGCCGATAC GGATCTCATC ATCGAAGTCG AGCGCGACCT CACCACCTAT GGCGAGGAAG TGAAGTTCGG CGGCGGCAAG GTTATCCGCG ACGGTATGGG CCAGAGCCAG CTTTCCCGCG CCGAAGGCGC GATGGACACC GTCATCACCA ATGCGCTGAT CCTCGACCAT AGCGGCATCT ACAAGGCGGA TATCGGGCTT CTCGACGGGC GCATCGCCCT CATCGGCAAG GCAGGCAATC CCGATACGCA GCCCGGCATC TCGATCATCA TCGGCCCCGG CACGGAGATC ATCGCTGGCG AAGGCAAGAT CGTCACCGCG GGCGGCATCG ATACCCATGT CCATTTCATC TCGCCGCAAC AGGTGGATGA GGCGCTGAAT GCGGGCATCA CCTGCATGGT TGGCGGCGGC ACCGGCCCCG CGCATGGCAC GCTTGCCACC ACCTGCACGC CTGGCCCCTG GCACATCGCC CGGCTGATCC AGTCCTTCGA TGGCCTGCCG ATGAATATCG GTGTGTTCGG CAAGGGCAAT GCCTCGCTGC CCGGCGCATT GGAAGAAATG GTTCGCGCCG GCGCCTGCGG CCTGAAACTG CATGAGGACT GGGGTTGCAC ACCGGCGGCT ATCGACAATT GCCTTTCGGT TGCCGATCAT TTCGACGTTC AGGTGGCGAT CCACACCGAT ACGCTCAATG AAGGTGGCTT CGTTGAAGAT ACGCTCAATG CCTTCAAGGG ACGCACGATC CACTCTTTCC ATACGGAAGG TGCGGGCGGT GGGCACGCGC CCGATATCAT CCGCGTTTGC CAATATCCGA ACGTGCTGCC TGCTTCGACC AACCCGACCC GGCCCTACAC GGTCAACACG ATTGCGGAAC ATCTCGACAT GCTGATGGTC TGCCATCACC TGTCGCCTGC AATCCCTGAG GATATCGCCT TTGCCGAAAG CCGCATCCGC AAGGAAACGA TTGCGGCGGA AGATATTCTG CACGACATGG GCGCCTTCTC GATCATTTCC TCCGACAGCC AGGCGATGGG CCGTGTGGGT GAAATGATCA TCCGCTGCTG GCAGACGGCA GACAAGATGA AGAAACAGCG CGGCAGCCTG CCTGACGATA GGCCGGGCAA CGACAATTAC CGCGCCCGCC GTTATATTGC CAAATATACG ATCAATCCTG CTATCGCGCA TGGCATGGCG CATGAGATCG GCTCCGTGGA AGTGGGCAAG CGTGCGGATC TGGTGCTGTG GAACCCGGCC TTCTTCGGTG TCAAACCGGA TATGGTGCTG CTTGGCGGCT GGATCGCCAC CGCCCCCATG GGCGATGCAA ACGGTTCCAT CCCCACTCCG CAGCCTATGC ATACCCGCCC CATGTTCGGC TCGTTCGGCA AGGCGCTCAC CAACACGTCC ATCACGTTTG TTTCGCAAGC AGCGATGGAT GAGGGCCTGC GTGAGAAGAT CGGCGTGGAC AAGCAGCTGG TGGCGGTCGT CAATACGCGC GGCGGCATCG GCAAGCATTC CATGATCCTC AACAATGCCA TGCCGCAGAT GGAAGTGGAC CCGGAAACCT ATGAAGTGCG TGCGGACGGC GAACTTCTCA CCTGTGAGCC GGTAGACGTG GTACCAATGG CGCAACGCTA CTTCCTGTTT

Details for BrabA.17716.b.B1.GE37966

HARVESTED ON:	5/12/2014
SEQUENCED ON:	5/4/2014
EXPECTED MW:	62kDa
OBSERVED MW:	65kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	86
PERCENT COVERAGE:	67

Validated AA Sequence

Validated NT Sequence

cnctanatat tttntttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccag caagaattag ccgcgccacc tatgcgcaaa tgttcggccc caccgttggc gacaaggtgc gccttgccga tacggatctc atcatcgaag tcgagcgcga cctcaccacc tatggcgagg aagtgaagtt cggcggcggc aaggttatcc gcgacggtat gggccagagc cagctttccc gcgccgaagg cgcgatggac accgtcatca ccaatgcgct gatcctcgac catagcggca tctacaaggc ggatatcggg cttctcgacg ggcgcatcgc cctcatcggc aaggcaggca atcccgatac gcagcccggc atctcgatca tcatcggccc cggcacggag atcatcgctg gcgaaggcaa gatcgtcacc gcgggcggca tcgataccca tgtccatttc atctcgccgc aacaggtgga tgaggcgctg aatgcgggca tcacctgcat ggttggcggc ggcaccggcc ccgcgcatgg cacgcttgcc accacctgca cgcctggccc ctggcacatc gcccggctga tccagtcctt cgatggcctg ccgatgaata tcggtgtgtt cggcaagggc aatgcctcgc tgcccggcgc attggaagaa atggttcgcg ccggcgcctg cggcctgaaa ctgcatgagg actggggttg cacaccggcg gctatcgaca attgcctttc ggttgccgat catttcgacg ttcaggtggc gatccacacc gatacgctca atgaaggtgg cttcgttgaa gatacgctca atgccttnnn gggacgcacg atccactctt tccatacgga aggtgcgggc ggtgggcacg cgcccgatat catccgcgtt tgccaatatc cgaacgtgct gcctgcttcg accaacccga cccggcccta cacggtcaac acgattgcgg aacatctcga cntgctgatg gtctgccatc acctgtcgcc tgcatccctg aggatatcgc ctttgccgaa agnncatcgc angaaacgat tgnnnngann atatntgcac gannatgggn ngcctnctcg atcatttctc gacannngnn ntggnnnnng gtggnnnnaa ngatcatcgc nnctggnnga anggnngaan nagnatgann aaaac

Expected Protein Sequence

MAHHHHHHMP ARISRATYAQ MFGPTVGDKV RLADTDLIIE VERDLTTYGE EVKFGGGKVI RDGMGQSQLS RAEGAMDTVI TNALILDHSG IYKADIGLLD GRIALIGKAG NPDTQPGISI IIGPGTEIIA GEGKIVTAGG IDTHVHFISP QQVDEALNAG ITCMVGGGTG PAHGTLATTC TPGPWHIARL IQSFDGLPMN IGVFGKGNAS LPGALEEMVR AGACGLKLHE DWGCTPAAID NCLSVADHFD VQVAIHTDTL NEGGFVEDTL NAFKGRTIHS FHTEGAGGGH APDIIRVCQY PNVLPASTNP TRPYTVNTIA EHLDMLMVCH HLSPAIPEDI AFAESRIRKE TIAAEDILHD MGAFSIISSD SQAMGRVGEM IIRCWQTADK MKKQRGSLPD DRPGNDNYRA RRYIAKYTIN PAIAHGMAHE IGSVEVGKRA DLVLWNPAFF GVKPDMVLLG GWIATAPMGD ANGSIPTPQP MHTRPMFGSF GKALTNTSIT FVSQAAMDEG LREKIGVDKQ LVAVVNTRGG IGKHSMILNN AMPQMEVDPE TYEVRADGEL LTCEPVDVVP MAQRYFLF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccag caagaattag ccgcgccacc tatgcgcaaa tgttcggccc caccgttggc gacaaggtgc gccttgccga tacggatctc atcatcgaag tcgagcgcga cctcaccacc tatggcgagg aagtgaagtt cggcggcggc aaggttatcc gcgacggtat gggccagagc cagctttccc gcgccgaagg cgcgatggac accgtcatca ccaatgcgct gatcctcgac catagcggca tctacaaggc ggatatcggg cttctcgacg ggcgcatcgc cctcatcggc aaggcaggca atcccgatac gcagcccggc atctcgatca tcatcggccc cggcacggag atcatcgctg gcgaaggcaa gatcgtcacc gcgggcggca tcgataccca tgtccatttc atctcgccgc aacaggtgga tgaggcgctg aatgcgggca tcacctgcat ggttggcggc ggcaccggcc ccgcgcatgg cacgcttgcc accacctgca cgcctggccc ctggcacatc gcccggctga tccagtcctt cgatggcctg ccgatgaata tcggtgtgtt cggcaagggc aatgcctcgc tgcccggcgc attggaagaa atggttcgcg ccggcgcctg cggcctgaaa ctgcatgagg actggggttg cacaccggcg gctatcgaca attgcctttc ggttgccgat catttcgacg ttcaggtggc gatccacacc gatacgctca atgaaggtgg cttcgttgaa gatacgctca atgccttcaa gggacgcacg atccactctt tccatacgga aggtgcgggc ggtgggcacg cgcccgatat catccgcgtt tgccaatatc cgaacgtgct gcctgcttcg accaacccga cccggcccta cacggtcaac acgattgcgg aacatctcga catgctgatg gtctgccatc acctgtcgcc tgcaatccct gaggatatcg cctttgccga aagccgcatc cgcaaggaaa cgattgcggc ggaagatatt ctgcacgaca tgggcgcctt ctcgatcatt tcctccgaca gccaggcgat gggccgtgtg ggtgaaatga tcatccgctg ctggcagacg gcagacaaga tgaagaaaca gcgcggcagc ctgcctgacg ataggccggg caacgacaat taccgcgccc gccgttatat tgccaaatat acgatcaatc ctgctatcgc gcatggcatg gcgcatgaga tcggctccgt ggaagtgggc aagcgtgcgg atctggtgct gtggaacccg gccttcttcg gtgtcaaacc ggatatggtg ctgcttggcg gctggatcgc caccgccccc atgggcgatg caaacggttc catccccact ccgcagccta tgcatacccg ccccatgttc ggctcgttcg gcaaggcgct caccaacacg tccatcacgt ttgtttcgca agcagcgatg gatgagggcc tgcgtgagaa gatcggcgtg gacaagcagc tggtggcggt cgtcaatacg cgcggcggca tcggcaagca ttccatgatc ctcaacaatg ccatgccgca gatggaagtg gacccggaaa cctatgaagt gcgtgcggac ggcgaacttc tcacctgtga gccggtagac gtggtaccaa tggcgcaacg ctacttcctg ttttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat