BrabA.17704.a

Periplasmic binding protein

CENTER ID: BrabA.17704.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17704.a.A2.GE32524 Mature protein( BrabA.17704.a ) 29 344
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.2779
RefSeq: YP_418677.1
UniProt: Q2YL07

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKIIKNRPFL AAFYSFAFLV TGFSGSSLAG ETRYPLTLKN CGREVTFHHA PQRAVAIGQS SAEILYNLGL GDRVVGTAVW FGPVMKGYEE VNARVKRLAD NDPSLESVLA QKSDMVAAQF QWHVGPEGIV AKPEQFEELG IPVYTAPADC IGKVNSGKGD GVRHTVFTMD LIYQEIRELA EIFNVQDRGE KVIADLKARE EAARKKIASA NGRLSAVVWF SSAQMDIDPY VAGKNGAPAY ILSALGIRNV IGSEEEWPTV GWETIAKSDP TMIIAGKMDR RRYPADDIAV KRKFLETDPV ASLMPAVKDG HVFDMDAQAM NPTIRTIEGI ETLADAIAAA GLNK
NT Sequence
ATGAAAATCA TAAAAAACAG GCCATTCCTT GCTGCTTTTT ATAGTTTCGC TTTTCTCGTT ACCGGCTTTT CAGGCTCTTC GCTTGCTGGT GAAACCCGCT ATCCGCTGAC GCTGAAAAAT TGCGGACGTG AGGTGACTTT CCATCACGCG CCGCAGCGTG CGGTCGCTAT CGGCCAGAGC AGCGCGGAAA TCCTCTATAA TCTGGGTCTC GGTGACAGGG TTGTAGGCAC GGCAGTCTGG TTCGGCCCGG TCATGAAGGG CTATGAGGAG GTGAACGCCA GGGTAAAGCG CCTTGCCGAT AATGATCCGA GCCTTGAAAG CGTCCTTGCA CAGAAGTCGG ATATGGTGGC CGCGCAATTC CAGTGGCATG TCGGCCCCGA AGGCATCGTC GCCAAACCGG AACAATTCGA GGAACTTGGC ATTCCCGTCT ATACCGCACC AGCAGATTGC ATCGGAAAGG TTAATTCCGG CAAGGGCGAC GGCGTGCGCC ACACTGTCTT CACCATGGAC CTGATCTATC AGGAAATCAG GGAGTTGGCG GAGATTTTCA ATGTGCAGGA TCGCGGCGAG AAGGTGATTG CCGATCTGAA GGCGCGCGAG GAAGCCGCCC GCAAGAAAAT CGCATCGGCC AATGGCAGGC TTTCCGCCGT CGTCTGGTTC TCCAGTGCGC AAATGGACAT TGATCCCTAT GTGGCAGGCA AGAACGGCGC GCCTGCCTAT ATTCTTTCCG CGCTCGGCAT CAGGAATGTG ATCGGCAGCG AGGAAGAATG GCCAACGGTC GGCTGGGAGA CCATCGCCAA GTCTGACCCG ACCATGATTA TTGCAGGCAA GATGGATCGC CGCCGCTACC CCGCCGATGA TATTGCCGTA AAACGCAAGT TTCTGGAGAC CGATCCGGTT GCAAGCCTGA TGCCTGCGGT GAAGGATGGC CATGTCTTCG ATATGGATGC GCAAGCCATG AACCCGACCA TCCGCACCAT AGAAGGCATT GAAACGCTGG CTGATGCCAT TGCCGCCGCC GGGCTGAACA AG
Details for BrabA.17704.a.A2.GE32524
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 89
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAGETRYPL TLKNCGREVT FHHAPQRAVA IGQSSAEILY NLGLGDRVVG TAVWFGPVMK GYEEVNARVK RLADNDPSLE SVLAQKSDMV AAQFQWHVGP EGIVAKPEQF EELGIPVYTA PADCIGKVNS GKGDGVRHTV FTMDLIYQEI RELAEIFNVQ DRGEKVIADL KAREEAARKK IASANGRLSA VVWFSSAQMD IDPYVAGKNG APAYILSALG IRNVIGSEEE WPTVGWETIA KSDPTMIXXG KMDRRRYPAD DIAVKRKFLE TDPVASLMPA V
Validated NT Sequence
ttcaccgcag gcatcaggct tgcaaccgga tcggtctcca gaaacttgcg ttttacggca atatcatcgg cggggtagcg gcggcgatcc atcttgccng nnnnaatcat ggtcgggtca gacttggcga tggtctccca gccgaccgtt ggccattctt cctcgctgcc gatcacattc ctgatgccga gcgcggaaag aatataggca ggcgcgccgt tcttgcctgc cacataggga tcaatgtcca tttgcgcact ggagaaccag acgacggcgg aaagcctgcc attggccgat gcgattttct tgcgggcggc ttcctcgcgc gccttcagat cggcaatcac cttctcgccg cgatcctgca cattgaaaat ctccgccaac tccctgattt cctgatagat caggtccatg gtgaagacag tgtggcgcac gccgtcgccc ttgccggaat taacctttcc gatgcaatct gctggtgcgg tatagacggg aatgccaagt tcctcgaatt gttccggttt ggcgacgatg ccttcggggc cgacatgcca ctggaattgc gcggccacca tatccgactt ctgtgcaagg acgctttcaa ggctcggatc attatcggca aggcgcttta ccctggcgtt cacctcctca tagcccttca tgaccgggcc gaaccagact gccgtgccta caaccctgtc accgagaccc agattataga ggatttccgc gctgctctgg ccgatagcga ccgcacgctg cggcgcgtga tggaaagtca cctcacgtcc gcaatttttc agcgtcagcg gatagcgggt ttcaccagcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAGETRYPL TLKNCGREVT FHHAPQRAVA IGQSSAEILY NLGLGDRVVG TAVWFGPVMK GYEEVNARVK RLADNDPSLE SVLAQKSDMV AAQFQWHVGP EGIVAKPEQF EELGIPVYTA PADCIGKVNS GKGDGVRHTV FTMDLIYQEI RELAEIFNVQ DRGEKVIADL KAREEAARKK IASANGRLSA VVWFSSAQMD IDPYVAGKNG APAYILSALG IRNVIGSEEE WPTVGWETIA KSDPTMIIAG KMDRRRYPAD DIAVKRKFLE TDPVASLMPA VKDGHVFDMD AQAMNPTIRT IEGIETLADA IAAAGLNK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctggtgaa acccgctatc cgctgacgct gaaaaattgc ggacgtgagg tgactttcca tcacgcgccg cagcgtgcgg tcgctatcgg ccagagcagc gcggaaatcc tctataatct gggtctcggt gacagggttg taggcacggc agtctggttc ggcccggtca tgaagggcta tgaggaggtg aacgccaggg taaagcgcct tgccgataat gatccgagcc ttgaaagcgt ccttgcacag aagtcggata tggtggccgc gcaattccag tggcatgtcg gccccgaagg catcgtcgcc aaaccggaac aattcgagga acttggcatt cccgtctata ccgcaccagc agattgcatc ggaaaggtta attccggcaa gggcgacggc gtgcgccaca ctgtcttcac catggacctg atctatcagg aaatcaggga gttggcggag attttcaatg tgcaggatcg cggcgagaag gtgattgccg atctgaaggc gcgcgaggaa gccgcccgca agaaaatcgc atcggccaat ggcaggcttt ccgccgtcgt ctggttctcc agtgcgcaaa tggacattga tccctatgtg gcaggcaaga acggcgcgcc tgcctatatt ctttccgcgc tcggcatcag gaatgtgatc ggcagcgagg aagaatggcc aacggtcggc tgggagacca tcgccaagtc tgacccgacc atgattattg caggcaagat ggatcgccgc cgctaccccg ccgatgatat tgccgtaaaa cgcaagtttc tggagaccga tccggttgca agcctgatgc ctgcggtgaa ggatggccat gtcttcgata tggatgcgca agccatgaac ccgaccatcc gcaccataga aggcattgaa acgctggctg atgccattgc cgccgccggg ctgaacaagt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa