BrabA.17695.a

Putative uncharacterized protein

CENTER ID: BrabA.17695.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17695.a.A1.GE31726 Full length( BrabA.17695.a ) 1 284
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17695.a.A1.PW31629 Full length( BrabA.17695.a ) 1 284

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.1954
RefSeq: YP_415221.1
UniProt: Q2YLN3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTFRAAAIQ MRSGIDVVRN VEALEKLVAD AAAQGAHYIQ TPEMTGALMR DRPALFASVR DEENDLVFRA ARALAARHGV FLHIGSTAID AGTGKIANRG GIFAPSGEKI ATYDKIHMFD VDLDNGESWR ESAACEPGRQ AVIAELPFAK VGMAICYDIR FPQLFQAQAL AGANVITDPA AFTRQTGEAH WHVLQRARAI ENGAFLISAA QGGLHEDGRE TYGHSIIVSP WGKVLAEAAH DEPGVIVADI DLSESTAARA KVPNLKNARE FEVSLVCASQ KEDA
NT Sequence
ATGAGCACCT TTCGCGCTGC CGCAATCCAG ATGCGTTCGG GCATTGATGT CGTCCGCAAT GTGGAGGCTC TCGAAAAACT GGTCGCAGAT GCGGCAGCGC AAGGCGCCCA TTATATCCAG ACGCCGGAAA TGACCGGCGC GCTGATGCGC GACCGTCCGG CCCTTTTTGC AAGCGTGCGC GATGAAGAGA ACGACCTGGT CTTCCGGGCG GCTCGCGCAC TGGCGGCCAG GCATGGCGTC TTCCTGCATA TCGGTTCGAC GGCAATCGAT GCAGGCACCG GCAAGATCGC CAATCGCGGC GGGATTTTTG CGCCATCGGG CGAAAAGATT GCCACTTATG ACAAGATTCA TATGTTCGAT GTCGATCTCG ACAATGGCGA AAGCTGGCGC GAATCGGCGG CCTGTGAGCC CGGCAGGCAA GCCGTCATCG CGGAACTGCC TTTCGCCAAA GTGGGCATGG CGATCTGCTA CGATATCCGT TTCCCGCAGC TTTTTCAGGC ACAGGCGCTG GCTGGCGCAA ATGTCATCAC CGACCCTGCT GCTTTCACGC GCCAGACTGG TGAAGCCCAT TGGCACGTTC TGCAGCGCGC ACGCGCTATC GAGAATGGCG CGTTCCTGAT TTCCGCGGCA CAGGGCGGGC TGCATGAAGA TGGCCGTGAA ACCTATGGTC ATTCGATTAT CGTTTCACCC TGGGGCAAGG TGCTGGCGGA GGCTGCCCAT GACGAACCCG GCGTGATCGT TGCCGATATC GACCTGTCGG AAAGCACGGC GGCACGCGCC AAGGTGCCGA ACCTGAAGAA TGCGCGGGAA TTTGAGGTGA GCCTAGTTTG TGCGAGCCAG AAAGAGGATG CG
Details for BrabA.17695.a.A1.GE31726
HARVESTED ON: 2/23/2011
SEQUENCED ON: 3/1/2011
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MGTLEAQTQG PGSMSTFRAA AIQMRSGIDV VRNVEALEKL VADAAAQGAH YIQTPEMTGA LMRDRPALFA SVRDEENDLV FRAARALAAR HGVFLHIGST AIDAGTGKIA NRGGIFAPSG EKIATYDKIH MFDVDLDNGE SWRESAACEP GRQAVIAELP FAKVGMAICY DIRFPQLFQA QALAGANVIT DPAAFTRQTG EAHWHVLQRA RAIENGAFLI SAAQGGLHED GRETYGHSII VSPWGKVLAE AAHDEPGVIV ADIDLSESTA ARAKVPNLKN AREFEVSLVC A
Validated NT Sequence
ctcgcacaaa ctaggctcac ctcaaattcc cgcgcattct tcaggttcgg caccttggcg cgtgccgccg tgctttccga caggtcgata tcggcaacga tcacgccggg ttcgtcatgg gcagcctccg ccagcacctt gccccagggt gaaacgataa tcgaatgacc ataggtttca cggccatctt catgcagccc gccctgtgcc gcggaaatca ggaacgcgcc attctcgata gcgcgtgcgc gctgcagaac gtgccaatgg gcttcaccag tctggcgcgt gaaagcagca gggtcggtga tgacatttgc gccagccagc gcctgtgcct gaaaaagctg cgggaaacgg atatcgtagc agatcgccat gcccactttg gcgaaaggca gttccgcgat gacggcttgc ctgccgggct cacaggccgc cgattcgcgc cagctttcgc cattgtcgag atcgacatcg aacatatgaa tcttgtcata agtggcaatc ttttcgcccg atggcgcaaa aatcccgccg cgattggcga tcttgccggt gcctgcatcg attgccgtcg aaccgatatg caggaagacg ccatgcctgg ccgccagtgc gcgagccgcc cggaagacca ggtcgttctc ttcatcgcgc acgcttgcaa aaagggccgg acggtcgcgc atcagcgcgc cggtcatttc cggcgtctgg atataatggg cgccttgcgc tgccgcatct gcgaccagtt tttcgagagc ctccacattg cggacgacat caatgcccga acgcatctgg attgcggcag cgcgaaaggt gctcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat gg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTFRAAAI QMRSGIDVVR NVEALEKLVA DAAAQGAHYI QTPEMTGALM RDRPALFASV RDEENDLVFR AARALAARHG VFLHIGSTAI DAGTGKIANR GGIFAPSGEK IATYDKIHMF DVDLDNGESW RESAACEPGR QAVIAELPFA KVGMAICYDI RFPQLFQAQA LAGANVITDP AAFTRQTGEA HWHVLQRARA IENGAFLISA AQGGLHEDGR ETYGHSIIVS PWGKVLAEAA HDEPGVIVAD IDLSESTAAR AKVPNLKNAR EFEVSLVCAS QKEDA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaccttt cgcgctgccg caatccagat gcgttcgggc attgatgtcg tccgcaatgt ggaggctctc gaaaaactgg tcgcagatgc ggcagcgcaa ggcgcccatt atatccagac gccggaaatg accggcgcgc tgatgcgcga ccgtccggcc ctttttgcaa gcgtgcgcga tgaagagaac gacctggtct tccgggcggc tcgcgcactg gcggccaggc atggcgtctt cctgcatatc ggttcgacgg caatcgatgc aggcaccggc aagatcgcca atcgcggcgg gatttttgcg ccatcgggcg aaaagattgc cacttatgac aagattcata tgttcgatgt cgatctcgac aatggcgaaa gctggcgcga atcggcggcc tgtgagcccg gcaggcaagc cgtcatcgcg gaactgcctt tcgccaaagt gggcatggcg atctgctacg atatccgttt cccgcagctt tttcaggcac aggcgctggc tggcgcaaat gtcatcaccg accctgctgc tttcacgcgc cagactggtg aagcccattg gcacgttctg cagcgcgcac gcgctatcga gaatggcgcg ttcctgattt ccgcggcaca gggcgggctg catgaagatg gccgtgaaac ctatggtcat tcgattatcg tttcaccctg gggcaaggtg ctggcggagg ctgcccatga cgaacccggc gtgatcgttg ccgatatcga cctgtcggaa agcacggcgg cacgcgccaa ggtgccgaac ctgaagaatg cgcgggaatt tgaggtgagc ctagtttgtg cgagccagaa agaggatgcg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BrabA.17695.a.A1.PW31629
PURIFICATION DATe: 4/8/2011
CONCENTRATION: 24.89mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 1000µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMSTFRAA AIQMRSGIDV VRNVEALEKL VADAAAQGAH YIQTPEMTGA LMRDRPALFA SVRDEENDLV FRAARALAAR HGVFLHIGST AIDAGTGKIA NRGGIFAPSG EKIATYDKIH MFDVDLDNGE SWRESAACEP GRQAVIAELP FAKVGMAICY DIRFPQLFQA QALAGANVIT DPAAFTRQTG EAHWHVLQRA RAIENGAFLI SAAQGGLHED GRETYGHSII VSPWGKVLAE AAHDEPGVIV ADIDLSESTA ARAKVPNLKN AREFEVSLVC A
Validated NT Sequence
ctcgcacaaa ctaggctcac ctcaaattcc cgcgcattct tcaggttcgg caccttggcg cgtgccgccg tgctttccga caggtcgata tcggcaacga tcacgccggg ttcgtcatgg gcagcctccg ccagcacctt gccccagggt gaaacgataa tcgaatgacc ataggtttca cggccatctt catgcagccc gccctgtgcc gcggaaatca ggaacgcgcc attctcgata gcgcgtgcgc gctgcagaac gtgccaatgg gcttcaccag tctggcgcgt gaaagcagca gggtcggtga tgacatttgc gccagccagc gcctgtgcct gaaaaagctg cgggaaacgg atatcgtagc agatcgccat gcccactttg gcgaaaggca gttccgcgat gacggcttgc ctgccgggct cacaggccgc cgattcgcgc cagctttcgc cattgtcgag atcgacatcg aacatatgaa tcttgtcata agtggcaatc ttttcgcccg atggcgcaaa aatcccgccg cgattggcga tcttgccggt gcctgcatcg attgccgtcg aaccgatatg caggaagacg ccatgcctgg ccgccagtgc gcgagccgcc cggaagacca ggtcgttctc ttcatcgcgc acgcttgcaa aaagggccgg acggtcgcgc atcagcgcgc cggtcatttc cggcgtctgg atataatggg cgccttgcgc tgccgcatct gcgaccagtt tttcgagagc ctccacattg cggacgacat caatgcccga acgcatctgg attgcggcag cgcgaaaggt gctcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat gg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTFRAAAI QMRSGIDVVR NVEALEKLVA DAAAQGAHYI QTPEMTGALM RDRPALFASV RDEENDLVFR AARALAARHG VFLHIGSTAI DAGTGKIANR GGIFAPSGEK IATYDKIHMF DVDLDNGESW RESAACEPGR QAVIAELPFA KVGMAICYDI RFPQLFQAQA LAGANVITDP AAFTRQTGEA HWHVLQRARA IENGAFLISA AQGGLHEDGR ETYGHSIIVS PWGKVLAEAA HDEPGVIVAD IDLSESTAAR AKVPNLKNAR EFEVSLVCAS QKEDA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCACCTTT CGCGCTGCCG CAATCCAGAT GCGTTCGGGC ATTGATGTCG TCCGCAATGT GGAGGCTCTC GAAAAACTGG TCGCAGATGC GGCAGCGCAA GGCGCCCATT ATATCCAGAC GCCGGAAATG ACCGGCGCGC TGATGCGCGA CCGTCCGGCC CTTTTTGCAA GCGTGCGCGA TGAAGAGAAC GACCTGGTCT TCCGGGCGGC TCGCGCACTG GCGGCCAGGC ATGGCGTCTT CCTGCATATC GGTTCGACGG CAATCGATGC AGGCACCGGC AAGATCGCCA ATCGCGGCGG GATTTTTGCG CCATCGGGCG AAAAGATTGC CACTTATGAC AAGATTCATA TGTTCGATGT CGATCTCGAC AATGGCGAAA GCTGGCGCGA ATCGGCGGCC TGTGAGCCCG GCAGGCAAGC CGTCATCGCG GAACTGCCTT TCGCCAAAGT GGGCATGGCG ATCTGCTACG ATATCCGTTT CCCGCAGCTT TTTCAGGCAC AGGCGCTGGC TGGCGCAAAT GTCATCACCG ACCCTGCTGC TTTCACGCGC CAGACTGGTG AAGCCCATTG GCACGTTCTG CAGCGCGCAC GCGCTATCGA GAATGGCGCG TTCCTGATTT CCGCGGCACA GGGCGGGCTG CATGAAGATG GCCGTGAAAC CTATGGTCAT TCGATTATCG TTTCACCCTG GGGCAAGGTG CTGGCGGAGG CTGCCCATGA CGAACCCGGC GTGATCGTTG CCGATATCGA CCTGTCGGAA AGCACGGCGG CACGCGCCAA GGTGCCGAAC CTGAAGAATG CGCGGGAATT TGAGGTGAGC CTAGTTTGTG CGAGCCAGAA AGAGGATGCG TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA