BrabA.17690.a
Basic membrane lipoprotein
| CENTER ID: | BrabA.17690.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.17690.a.A2.GE31724 | Mature protein( BrabA.17690.a ) | 17 | 350 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.17690.a.A2.PS01053 | Mature protein( BrabA.17690.a ) | 17 | 350 |
Structures
3S99
DEPOSITED: 6/1/2011
DETERMINATION: XRay
CLONE: BrabA.17690.a.A2.GE31724
PROTEIN: BrabA.17690.a.A2.PS01053
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_155553 |
| PATRIC ID: | fig|359391.4.peg.2968 |
| RefSeq: | YP_418848.1 |
| UniProt: | Q2YKI6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVIATVAGFM LGGAAHAEEK LKVGFIYIGP PGDFGWTYQH DQARKELVEA LGDKVETTFL ENVAEGADAE RSIKRIARAG NKLIFTTSFG YMDPTVKVAK KFPDVKFEHA TGYKTADNMS AYNARFYEGR YVQGVIAAKM SKKGIAGYIG SVPVPEVVQG INSFMLGAQS VNPDFRVKVI WVNSWFDPGK EADAAKALID QGVDIITQHT DSTAAIQVAH DRGIKAFGQA SDMIKFAPDT QLTAVVDEWG PYYIDRAKAV LDGTWKSQNI WWGMKEGLVK MAPFTNMPDD VKKLAEETEA RIKSGELNPF TGPIKKQDGS EWLKAGEKAD DQTLLGMNFY VAGVDDKLPQ
NT Sequence
ATGGTAATTG CTACTGTCGC GGGCTTTATG CTCGGCGGGG CGGCACATGC AGAAGAAAAA CTGAAGGTGG GCTTCATCTA TATCGGCCCT CCCGGCGATT TCGGCTGGAC GTACCAGCAT GATCAGGCGC GCAAGGAGCT GGTCGAGGCG CTCGGCGACA AGGTTGAAAC CACCTTTCTT GAAAACGTAG CCGAAGGTGC GGATGCCGAG CGCTCCATCA AACGTATTGC CCGCGCAGGC AACAAGCTGA TCTTCACAAC CTCCTTCGGT TACATGGACC CGACCGTGAA GGTCGCCAAG AAGTTCCCGG ACGTGAAGTT TGAACATGCA ACCGGCTACA AGACCGCAGA CAACATGTCC GCCTATAATG CGCGCTTCTA TGAAGGCCGT TATGTGCAGG GCGTGATTGC CGCAAAGATG TCGAAGAAGG GCATTGCCGG CTATATCGGC TCCGTGCCGG TGCCGGAAGT CGTGCAGGGC ATCAACTCCT TCATGCTGGG CGCACAGTCC GTCAATCCTG ATTTCAGGGT CAAGGTAATC TGGGTCAATT CCTGGTTCGA TCCGGGCAAG GAAGCCGATG CCGCCAAGGC GCTGATTGAT CAGGGCGTGG ATATCATCAC CCAGCACACG GATTCCACCG CCGCCATTCA GGTGGCGCAC GACCGCGGCA TCAAGGCGTT CGGCCAGGCT TCGGACATGA TCAAGTTTGC GCCTGACACG CAGCTTACCG CTGTTGTTGA CGAATGGGGT CCGTACTATA TCGACCGCGC CAAGGCTGTT CTTGACGGTA CGTGGAAAAG CCAGAACATC TGGTGGGGTA TGAAGGAAGG CCTCGTGAAG ATGGCGCCTT TCACCAATAT GCCAGATGAT GTGAAGAAGC TGGCGGAAGA AACCGAAGCC AGGATCAAGT CTGGTGAACT CAATCCCTTC ACCGGCCCGA TCAAGAAGCA AGACGGTTCG GAATGGCTGA AAGCAGGCGA AAAGGCTGAT GACCAGACCC TGCTTGGCAT GAATTTCTAC GTTGCTGGCG TGGATGATAA GCTGCCGCAA
Details for BrabA.17690.a.A2.GE31724
| HARVESTED ON: | 2/23/2011 |
| SEQUENCED ON: | 3/1/2011 |
| EXPECTED MW: | 39kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 95 |
| PERCENT COVERAGE: | 92 |
Validated AA Sequence
MXXEKLKVGF IYIGPPGDFG WTYQHDQAXX XXVEALGDKV ETTFLXXXXE GADAERSIKR IARAGXKLIF TTXFGYXDPT VKVAKKFPDV KFEHATGYKT ADNMSAYNAR FYEGRYVQGV IAAKMSKKGI AGYIGSVPVP EVVQGINSFM LGAQSVXPDF RVKVIWVNSW FDPGKEADAA KALIDQGVDI ITQHTDSTAA IQVAHDRGIK AFGQASDMIK FAPDTQLTAV VDEWGPYYID RAKAVLDGTW KSQNIWWGMK EGLVKMAPFT NMPDDVKKLA EETEARIKSG ELNPFTGPIK KQDGSEWLKA GEKADDQTLL GMNFYVAGV
Validated NT Sequence
ccacgccagc aacgtagaaa ttcatgccaa gcagggtctg gtcatcagcc ttttcgcctg ctttcagcca ttccgaaccg tcttgcttct tgatcgggcc ggtgaaggga ttgagttcac cagacttgat cctggcttcg gtttcttccg ccagcttctt cacatcatca ggcatattgg tgaaaggcgc catcttcacg aggccttcct tcatacccca ccagatgttc tggcttttcc acgtaccgtc aagaacagcc ttggcgcggt cgatatagta cggaccccat tcgtcaacaa cagcggtaag ctgcgtgtca ggcgcaaact tgatcatgtc cgaagcctgg ccgaacgcct tgatgccgcg gtcgtgcgcc acctgaatgg cggcggtgga atccgtgtgc tgggtgatga tatccacgcc ctgatcaatc agcgccttgg cggcatcggc ttccttgccc ggatcgaacc aggaattgac ccagattacc ttgaccctga aatcaggant gacggactgt gcgcccagca tgaaggagtt gatgccctgc acgacttccg gcaccggcac ggagccgata tagccggcaa tgcccttctt cgacatcttt gcggcaatca cgccctgcac ataacggcct tcatagaagc gcgcattata ngcggacatg ttgtctgcgg tcttgtagcc ngttgcatgt tcaaacttca cgtccgggaa cttcttggcg accttcacgg tcgggtccnt gtaaccgaan nnngttgtga agatcagctt gntgcctgcg cgggcaatac gtttgatgga gcgctcggca tccgcacctt cggnnnnnnn ntcaagaaag gtggtttcaa ccttgtcgcc gagcgcctcg accnnnnnnn nnnnngcctg atcatgctgg tacgtccagc cgaaatcgcc gggagggccg atatagatga ancccacctt cagtttttct tnnnccatcg aaccaggacc ctgggt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAEEKLKVG FIYIGPPGDF GWTYQHDQAR KELVEALGDK VETTFLENVA EGADAERSIK RIARAGNKLI FTTSFGYMDP TVKVAKKFPD VKFEHATGYK TADNMSAYNA RFYEGRYVQG VIAAKMSKKG IAGYIGSVPV PEVVQGINSF MLGAQSVNPD FRVKVIWVNS WFDPGKEADA AKALIDQGVD IITQHTDSTA AIQVAHDRGI KAFGQASDMI KFAPDTQLTA VVDEWGPYYI DRAKAVLDGT WKSQNIWWGM KEGLVKMAPF TNMPDDVKKL AEETEARIKS GELNPFTGPI KKQDGSEWLK AGEKADDQTL LGMNFYVAGV DDKLPQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcagaagaa aaactgaagg tgggcttcat ctatatcggc cctcccggcg atttcggctg gacgtaccag catgatcagg cgcgcaagga gctggtcgag gcgctcggcg acaaggttga aaccaccttt cttgaaaacg tagccgaagg tgcggatgcc gagcgctcca tcaaacgtat tgcccgcgca ggcaacaagc tgatcttcac aacctccttc ggttacatgg acccgaccgt gaaggtcgcc aagaagttcc cggacgtgaa gtttgaacat gcaaccggct acaagaccgc agacaacatg tccgcctata atgcgcgctt ctatgaaggc cgttatgtgc agggcgtgat tgccgcaaag atgtcgaaga agggcattgc cggctatatc ggctccgtgc cggtgccgga agtcgtgcag ggcatcaact ccttcatgct gggcgcacag tccgtcaatc ctgatttcag ggtcaaggta atctgggtca attcctggtt cgatccgggc aaggaagccg atgccgccaa ggcgctgatt gatcagggcg tggatatcat cacccagcac acggattcca ccgccgccat tcaggtggcg cacgaccgcg gcatcaaggc gttcggccag gcttcggaca tgatcaagtt tgcgcctgac acgcagctta ccgctgttgt tgacgaatgg ggtccgtact atatcgaccg cgccaaggct gttcttgacg gtacgtggaa aagccagaac atctggtggg gtatgaagga aggcctcgtg aagatggcgc ctttcaccaa tatgccagat gatgtgaaga agctggcgga agaaaccgaa gccaggatca agtctggtga actcaatccc ttcaccggcc cgatcaagaa gcaagacggt tcggaatggc tgaaagcagg cgaaaaggct gatgaccaga ccctgcttgg catgaatttc tacgttgctg gcgtggatga taagctgccg caataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BrabA.17690.a.A2.PS01053
| PURIFICATION DATe: | 4/20/2011 |
| CONCENTRATION: | 37.04mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 95 |
| PERCENT COVERAGE: | 92 |
Protocol Notes
notes unavailable
Validated AA Sequence
MXXEKLKVGF IYIGPPGDFG WTYQHDQAXX XXVEALGDKV ETTFLXXXXE GADAERSIKR IARAGXKLIF TTXFGYXDPT VKVAKKFPDV KFEHATGYKT ADNMSAYNAR FYEGRYVQGV IAAKMSKKGI AGYIGSVPVP EVVQGINSFM LGAQSVXPDF RVKVIWVNSW FDPGKEADAA KALIDQGVDI ITQHTDSTAA IQVAHDRGIK AFGQASDMIK FAPDTQLTAV VDEWGPYYID RAKAVLDGTW KSQNIWWGMK EGLVKMAPFT NMPDDVKKLA EETEARIKSG ELNPFTGPIK KQDGSEWLKA GEKADDQTLL GMNFYVAGV
Validated NT Sequence
ccacgccagc aacgtagaaa ttcatgccaa gcagggtctg gtcatcagcc ttttcgcctg ctttcagcca ttccgaaccg tcttgcttct tgatcgggcc ggtgaaggga ttgagttcac cagacttgat cctggcttcg gtttcttccg ccagcttctt cacatcatca ggcatattgg tgaaaggcgc catcttcacg aggccttcct tcatacccca ccagatgttc tggcttttcc acgtaccgtc aagaacagcc ttggcgcggt cgatatagta cggaccccat tcgtcaacaa cagcggtaag ctgcgtgtca ggcgcaaact tgatcatgtc cgaagcctgg ccgaacgcct tgatgccgcg gtcgtgcgcc acctgaatgg cggcggtgga atccgtgtgc tgggtgatga tatccacgcc ctgatcaatc agcgccttgg cggcatcggc ttccttgccc ggatcgaacc aggaattgac ccagattacc ttgaccctga aatcaggant gacggactgt gcgcccagca tgaaggagtt gatgccctgc acgacttccg gcaccggcac ggagccgata tagccggcaa tgcccttctt cgacatcttt gcggcaatca cgccctgcac ataacggcct tcatagaagc gcgcattata ngcggacatg ttgtctgcgg tcttgtagcc ngttgcatgt tcaaacttca cgtccgggaa cttcttggcg accttcacgg tcgggtccnt gtaaccgaan nnngttgtga agatcagctt gntgcctgcg cgggcaatac gtttgatgga gcgctcggca tccgcacctt cggnnnnnnn ntcaagaaag gtggtttcaa ccttgtcgcc gagcgcctcg accnnnnnnn nnnnngcctg atcatgctgg tacgtccagc cgaaatcgcc gggagggccg atatagatga ancccacctt cagtttttct tnnnccatcg aaccaggacc ctgggt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAEEKLKVG FIYIGPPGDF GWTYQHDQAR KELVEALGDK VETTFLENVA EGADAERSIK RIARAGNKLI FTTSFGYMDP TVKVAKKFPD VKFEHATGYK TADNMSAYNA RFYEGRYVQG VIAAKMSKKG IAGYIGSVPV PEVVQGINSF MLGAQSVNPD FRVKVIWVNS WFDPGKEADA AKALIDQGVD IITQHTDSTA AIQVAHDRGI KAFGQASDMI KFAPDTQLTA VVDEWGPYYI DRAKAVLDGT WKSQNIWWGM KEGLVKMAPF TNMPDDVKKL AEETEARIKS GELNPFTGPI KKQDGSEWLK AGEKADDQTL LGMNFYVAGV DDKLPQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCAGAAGAA AAACTGAAGG TGGGCTTCAT CTATATCGGC CCTCCCGGCG ATTTCGGCTG GACGTACCAG CATGATCAGG CGCGCAAGGA GCTGGTCGAG GCGCTCGGCG ACAAGGTTGA AACCACCTTT CTTGAAAACG TAGCCGAAGG TGCGGATGCC GAGCGCTCCA TCAAACGTAT TGCCCGCGCA GGCAACAAGC TGATCTTCAC AACCTCCTTC GGTTACATGG ACCCGACCGT GAAGGTCGCC AAGAAGTTCC CGGACGTGAA GTTTGAACAT GCAACCGGCT ACAAGACCGC AGACAACATG TCCGCCTATA ATGCGCGCTT CTATGAAGGC CGTTATGTGC AGGGCGTGAT TGCCGCAAAG ATGTCGAAGA AGGGCATTGC CGGCTATATC GGCTCCGTGC CGGTGCCGGA AGTCGTGCAG GGCATCAACT CCTTCATGCT GGGCGCACAG TCCGTCAATC CTGATTTCAG GGTCAAGGTA ATCTGGGTCA ATTCCTGGTT CGATCCGGGC AAGGAAGCCG ATGCCGCCAA GGCGCTGATT GATCAGGGCG TGGATATCAT CACCCAGCAC ACGGATTCCA CCGCCGCCAT TCAGGTGGCG CACGACCGCG GCATCAAGGC GTTCGGCCAG GCTTCGGACA TGATCAAGTT TGCGCCTGAC ACGCAGCTTA CCGCTGTTGT TGACGAATGG GGTCCGTACT ATATCGACCG CGCCAAGGCT GTTCTTGACG GTACGTGGAA AAGCCAGAAC ATCTGGTGGG GTATGAAGGA AGGCCTCGTG AAGATGGCGC CTTTCACCAA TATGCCAGAT GATGTGAAGA AGCTGGCGGA AGAAACCGAA GCCAGGATCA AGTCTGGTGA ACTCAATCCC TTCACCGGCC CGATCAAGAA GCAAGACGGT TCGGAATGGC TGAAAGCAGG CGAAAAGGCT GATGACCAGA CCCTGCTTGG CATGAATTTC TACGTTGCTG GCGTGGATGA TAAGCTGCCG CAATAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA