SSGCID: Target Page

BrabA.17684.a

Flagellar hook-associated protein FlgL, putative KO: K02397 flagellar hook-associated protein 3 FlgL

CENTER ID:	BrabA.17684.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17684.a.A1.GE31723	Full length( BrabA.17684.a )		1	348

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17684.a.A1.PS01060	Full length( BrabA.17684.a )		1	348

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.3399
RefSeq:	YP_419223.1
UniProt:	Q2YJG1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKAQSISTYG ATSALRALVA KNKAEMVKAQ QEATTGTVFD VGLSLGSRTG QTVSLRKEYD RLSVLTDMNK LVQQRMTATQ TAAGKIIENT QNFLGDLAGA NNSGETAKTV AKSARSMLDS VTGLLNTSFN GEYIFAGVNT DVKPISDYAD GSTAQNAVRQ AFQDHFGFAM DDPQVANISG DEMKAFLEGD FAEQFNDANW AANWSDASDT RIKSRISPTE TADTSISANA DGFRKTVMSA VMVTEFADIG LNASAFDALT TQALQITTQA VTETTSEQTT LGLAQSRTEA ATTRIAAQQK ILNQSVLNLE EVDPYDAATR VNALKTQIET SYSLTVQLQN MSLLNYLR

NT Sequence

ATGAAAGCGC AATCGATTTC CACCTATGGC GCAACATCAG CCCTGCGGGC CCTTGTCGCC AAGAACAAGG CTGAAATGGT GAAGGCACAG CAGGAAGCAA CGACCGGCAC TGTGTTCGAT GTCGGGCTGT CGCTGGGTTC TCGGACGGGG CAGACGGTTT CGCTGCGCAA GGAATATGAC CGGCTGAGCG TTTTGACCGA CATGAACAAG CTGGTCCAGC AGCGCATGAC CGCAACGCAG ACCGCAGCCG GCAAGATCAT CGAGAATACG CAGAACTTCC TTGGTGATCT GGCAGGGGCC AATAATTCCG GTGAAACGGC GAAGACCGTC GCTAAATCGG CGCGCTCGAT GCTGGATTCC GTTACCGGTC TTCTCAACAC CAGCTTCAAT GGCGAATATA TTTTCGCCGG TGTGAATACC GATGTGAAGC CCATCTCCGA TTATGCGGAT GGCAGCACCG CGCAAAATGC CGTGCGCCAG GCCTTTCAGG ATCATTTCGG TTTCGCCATG GATGATCCGC AGGTGGCGAA TATCAGCGGC GACGAGATGA AAGCCTTTCT GGAAGGTGAT TTCGCAGAAC AGTTCAACGA TGCCAATTGG GCGGCCAACT GGTCGGATGC TTCCGACACG CGCATCAAGA GCCGGATTTC GCCCACTGAA ACAGCGGATA CCTCCATTTC CGCCAATGCG GATGGCTTTC GCAAGACGGT CATGTCGGCG GTTATGGTGA CGGAATTCGC AGATATCGGC CTCAACGCTT CGGCTTTCGA TGCCCTGACA ACACAGGCCT TGCAGATCAC GACACAGGCG GTCACCGAAA CGACATCGGA GCAGACGACG CTCGGCCTTG CCCAGTCTCG CACCGAGGCC GCGACGACGC GCATTGCCGC GCAGCAGAAG ATCCTGAACC AGTCCGTGCT CAATCTTGAA GAGGTCGATC CCTATGACGC CGCAACGCGT GTGAACGCGC TCAAGACGCA GATCGAGACA TCTTATTCGC TGACGGTTCA ACTACAGAAT ATGAGCCTCC TCAACTATCT GAGG

Details for BrabA.17684.a.A1.GE31723

HARVESTED ON:	2/23/2011
SEQUENCED ON:	3/1/2011
EXPECTED MW:	39kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

attacctcag atagttgagg aggctcatat tctgtagttg aaccgtcagc gaataagatg tctcgatctg cgtcttgagc gcgttcacac gcgttgcggc gtcataggga tcgacctctt caagattgag cacggactgg ttcaggatct tctgctgcgc ggcaatgcgc gtcgtcgcgg cctcggtgcg agactgggca aggccgagcg tcgtctgctc cgatgtcgtt tcggtgaccg cctgtgtcgt gatctgcaag gcctgtgttg tcagggcatc gaaagccgaa gcgttgaggc cgatatctgc gaattccgtc accataaccg ccgacatgac cgtcttgcga aagccatccg cattggcgga aatggaggta tccgctgttt cagtgggcga aatccggctc ttgatgcgcg tgtcggaagc atccgaccag ttggccgccc aattggcatc gttgaactgt tctgcgaaat caccttccag aaaggctttc atctcgtcgc cgctgatatt cgccacctgc ggatcatcca tggcgaaacc gaaatgatcc tgaaaggcct ggcgcacggc attttgcgcg gtgctgccat ccgcataatc ggagatgggc ttcacatcgg tattcacacc ggcgaaaata tattcgccat tgaagctggt gttgagaaga ccggtaacgg aatccagcat cgagcgcgcc gatttagcga cggtcttcgc cgtttcaccg gaattattgg cccctgccag atcaccaagg aagttctgcg tattctcgat gatcttgccg gctgcggtct gcgttgcggt catgcgctgc tggaccagct tgttcatgtc ggtcaaaacg ctcagccggt catattcctt gcgcagcgaa accgtctgcc ccgtccgaga acccagcgac agcccgacat cgaacacagt gccggtcgtt gcttcctgct gtgccttcac catttcagcc ttgttcttgg cgacaagggc ccgcagggct gatgttgcgc cataggtgga aatcgattgc gctttcatcg aaccaggacc ctgggtctga gcttccag

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKAQSISTY GATSALRALV AKNKAEMVKA QQEATTGTVF DVGLSLGSRT GQTVSLRKEY DRLSVLTDMN KLVQQRMTAT QTAAGKIIEN TQNFLGDLAG ANNSGETAKT VAKSARSMLD SVTGLLNTSF NGEYIFAGVN TDVKPISDYA DGSTAQNAVR QAFQDHFGFA MDDPQVANIS GDEMKAFLEG DFAEQFNDAN WAANWSDASD TRIKSRISPT ETADTSISAN ADGFRKTVMS AVMVTEFADI GLNASAFDAL TTQALQITTQ AVTETTSEQT TLGLAQSRTE AATTRIAAQQ KILNQSVLNL EEVDPYDAAT RVNALKTQIE TSYSLTVQLQ NMSLLNYLR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaagcgcaa tcgatttcca cctatggcgc aacatcagcc ctgcgggccc ttgtcgccaa gaacaaggct gaaatggtga aggcacagca ggaagcaacg accggcactg tgttcgatgt cgggctgtcg ctgggttctc ggacggggca gacggtttcg ctgcgcaagg aatatgaccg gctgagcgtt ttgaccgaca tgaacaagct ggtccagcag cgcatgaccg caacgcagac cgcagccggc aagatcatcg agaatacgca gaacttcctt ggtgatctgg caggggccaa taattccggt gaaacggcga agaccgtcgc taaatcggcg cgctcgatgc tggattccgt taccggtctt ctcaacacca gcttcaatgg cgaatatatt ttcgccggtg tgaataccga tgtgaagccc atctccgatt atgcggatgg cagcaccgcg caaaatgccg tgcgccaggc ctttcaggat catttcggtt tcgccatgga tgatccgcag gtggcgaata tcagcggcga cgagatgaaa gcctttctgg aaggtgattt cgcagaacag ttcaacgatg ccaattgggc ggccaactgg tcggatgctt ccgacacgcg catcaagagc cggatttcgc ccactgaaac agcggatacc tccatttccg ccaatgcgga tggctttcgc aagacggtca tgtcggcggt tatggtgacg gaattcgcag atatcggcct caacgcttcg gctttcgatg ccctgacaac acaggccttg cagatcacga cacaggcggt caccgaaacg acatcggagc agacgacgct cggccttgcc cagtctcgca ccgaggccgc gacgacgcgc attgccgcgc agcagaagat cctgaaccag tccgtgctca atcttgaaga ggtcgatccc tatgacgccg caacgcgtgt gaacgcgctc aagacgcaga tcgagacatc ttattcgctg acggttcaac tacagaatat gagcctcctc aactatctga ggtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for BrabA.17684.a.A1.PS01060

PURIFICATION DATe:	4/22/2011
CONCENTRATION:	7.7mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAAGCGCAA TCGATTTCCA CCTATGGCGC AACATCAGCC CTGCGGGCCC TTGTCGCCAA GAACAAGGCT GAAATGGTGA AGGCACAGCA GGAAGCAACG ACCGGCACTG TGTTCGATGT CGGGCTGTCG CTGGGTTCTC GGACGGGGCA GACGGTTTCG CTGCGCAAGG AATATGACCG GCTGAGCGTT TTGACCGACA TGAACAAGCT GGTCCAGCAG CGCATGACCG CAACGCAGAC CGCAGCCGGC AAGATCATCG AGAATACGCA GAACTTCCTT GGTGATCTGG CAGGGGCCAA TAATTCCGGT GAAACGGCGA AGACCGTCGC TAAATCGGCG CGCTCGATGC TGGATTCCGT TACCGGTCTT CTCAACACCA GCTTCAATGG CGAATATATT TTCGCCGGTG TGAATACCGA TGTGAAGCCC ATCTCCGATT ATGCGGATGG CAGCACCGCG CAAAATGCCG TGCGCCAGGC CTTTCAGGAT CATTTCGGTT TCGCCATGGA TGATCCGCAG GTGGCGAATA TCAGCGGCGA CGAGATGAAA GCCTTTCTGG AAGGTGATTT CGCAGAACAG TTCAACGATG CCAATTGGGC GGCCAACTGG TCGGATGCTT CCGACACGCG CATCAAGAGC CGGATTTCGC CCACTGAAAC AGCGGATACC TCCATTTCCG CCAATGCGGA TGGCTTTCGC AAGACGGTCA TGTCGGCGGT TATGGTGACG GAATTCGCAG ATATCGGCCT CAACGCTTCG GCTTTCGATG CCCTGACAAC ACAGGCCTTG CAGATCACGA CACAGGCGGT CACCGAAACG ACATCGGAGC AGACGACGCT CGGCCTTGCC CAGTCTCGCA CCGAGGCCGC GACGACGCGC ATTGCCGCGC AGCAGAAGAT CCTGAACCAG TCCGTGCTCA ATCTTGAAGA GGTCGATCCC TATGACGCCG CAACGCGTGT GAACGCGCTC AAGACGCAGA TCGAGACATC TTATTCGCTG ACGGTTCAAC TACAGAATAT GAGCCTCCTC AACTATCTGA GGTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA