SSGCID: Target Page

BrabA.17683.a

3-dehydroquinate synthase

CENTER ID:	BrabA.17683.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17683.a.A1.GE32522	Full length( BrabA.17683.a )		1	378

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.2111
RefSeq:	YP_415359.1
UniProt:	Q2YR43

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNAPTTFADS VTVPVSLGDR SYDILIGKGL VERAGEEVAK RLKGVRVAIV TDENVAAVHL ERLQASFARA GIDSTPVIVA PGEKSKSFAT LETVTNAILV AKLERGDAVV ALGGGVVGDL SGFVAGIVRR GMNFVQMPTS LLAQVDSSVG GKTGINTAHG KNLVGVFNQP QLVLADTQVL DTLSPREFRA GYAEVAKYGL IDRPDFFAWL EANWQEVFSG GAARTKAIAE SCRSKAAVVA RDERETGDRA LLNLGHTFGH ALESATGYDS SRLVHGEGVA IGMALAYRFS ARMNLAGIEA AERVEAHLKA VGLPVSLAEV PGGLPPAEKL MDYIAQDKKV TRGTLTFILT HGIGQSFIAK DVPPAAVLEF LKERLAIA

NT Sequence

ATGAATGCGC CCACAACCTT TGCCGATAGT GTTACCGTTC CGGTTTCGCT GGGGGATCGT TCCTACGATA TTCTGATCGG CAAGGGGCTT GTCGAGCGCG CGGGCGAGGA AGTCGCCAAG CGGCTGAAGG GGGTTCGTGT CGCCATCGTC ACCGATGAGA ATGTGGCGGC AGTGCATCTT GAGCGTTTGC AGGCAAGCTT TGCACGGGCC GGTATCGATT CCACGCCGGT GATTGTCGCA CCTGGAGAAA AATCCAAATC CTTTGCGACG CTTGAAACCG TCACCAACGC CATTCTGGTG GCAAAGCTGG AGCGTGGCGA TGCGGTGGTG GCGTTGGGCG GTGGCGTGGT GGGCGACCTT TCCGGCTTTG TTGCGGGGAT CGTGCGGCGC GGCATGAATT TCGTGCAAAT GCCAACCTCG CTTCTTGCAC AGGTCGATTC CTCCGTTGGC GGCAAGACCG GCATTAATAC CGCCCATGGC AAGAACCTCG TTGGTGTCTT TAACCAGCCG CAACTCGTGC TGGCCGATAC GCAGGTGCTC GATACGCTGA GCCCGCGTGA ATTCCGTGCC GGTTATGCGG AAGTCGCAAA ATATGGCCTG ATCGACCGCC CGGATTTCTT CGCCTGGCTT GAAGCGAACT GGCAGGAGGT TTTCTCCGGC GGTGCGGCCC GCACAAAGGC TATTGCCGAA TCCTGCCGCT CAAAGGCTGC GGTCGTGGCG CGCGATGAGC GTGAAACCGG CGACCGCGCC CTGCTCAATC TCGGTCATAC ATTCGGCCAT GCGCTGGAAA GCGCAACCGG CTATGATTCC AGCCGTCTCG TTCATGGCGA AGGGGTCGCG ATCGGCATGG CGCTGGCATA TCGCTTTTCG GCCCGGATGA ACCTTGCCGG TATTGAAGCG GCAGAGCGGG TGGAAGCGCA TCTGAAAGCT GTCGGCCTGC CGGTTTCGCT GGCCGAGGTG CCGGGCGGCC TGCCGCCCGC GGAAAAGCTG ATGGATTATA TCGCGCAGGA CAAGAAGGTG ACGCGCGGTA CGCTGACCTT CATTCTCACG CATGGCATCG GCCAGTCCTT CATCGCAAAG GACGTACCGC CTGCTGCCGT GCTGGAATTT CTGAAAGAGC GCCTCGCCAT AGCT

Details for BrabA.17683.a.A1.GE32522

HARVESTED ON:	5/17/2011
SEQUENCED ON:	5/23/2011
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	95
PERCENT COVERAGE:	78

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNAPTTFAD SVTVPVSLGD RSYDILIGKG LVERAGEEVA KRLKGVRVAI VTDENVAAVH LERLQASFAR AGIDSTPVIV APGEKSKSFA TLETVTNAIL VAKLERGDAV VALGGGVVGD LSGFVAGIVR RGMNFVQMPT SLLAQVDSSV GGKTGINTAH GKNLVGVFNQ PQLVLADTQV LDTLSPREFR AGYAEVAKYG LIDRPDFFAW LEANWQEVFS GGAARTKAIA ESCRSKAAVV ARDERETGDR ALLNLGHTFG HALESATGYD SSRXVHGXGS RSAWRWHIAF RPX

Validated NT Sequence

tnnnnnnnnn nnnnaggaga nataccatgg ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tgaatgcgcc cacaaccttt gccgatagtg ttaccgttcc ggtttcgctg ggggatcgtt cctacgatat tctgatcggc aaggggcttg tcgagcgcgc gggcgaggaa gtcgccaagc ggctgaaggg ggttcgtgtc gccatcgtca ccgatgagaa tgtggcggca gtgcatcttg agcgtttgca ggcaagcttt gcacgggccg gtatcgattc cacgccggtg attgtcgcac ctggagaaaa atccaaatcc tttgcgacgc ttgaaaccgt caccaacgcc attctggtgg caaagctgga gcgtggcgat gcggtggtgg cgttgggcgg tggcgtggtg ggcgaccttt ccggctttgt tgcggggatc gtgcggcgcg gcatgaattt cgtgcaaatg ccaacctcgc ttcttgcaca ggtcgattcc tccgttggcg gcaagaccgg cattaatacc gcccatggca agaacctcgt tggtgtcttt aaccagccgc aactcgtgct ggccgatacg caggtgctcg atacgctgag cccgcgtgaa ttccgtgccg gttatgcgga agtcgcaaaa tatggcctga tcgaccgccc ggatttcttc gcctggcttg aagcgaactg gcaggaggtt ttctccggcg gtgcggcccg cacaaaggct attgccgaat cctgccgctc aaaggctgcg gtcgtggcgc gcgatgagcg tgaaaccggc gaccgcgccc tgctcaatct cggtcataca ttcggccatg cgctggaaag cgcaaccggc tatgattcca gccgtcncgt tcatggcaan gggtcgcgat cggcatggcg ctggcatatc gcttttcggc ccnnatgaan nntnnnntan tgaagcggca gancgggnnn nngcgcatct gaaagctgtn nncntnnnnn nngctnnnna ngnnnngnnn gnnncnnncn gctgannnnn nnnntcnnnc ngnnnnnnag nnnnnncnnn nnnnncnnan nnnnnnnnnc ncnnnnntnn nnnnncnttn nnnnnnnnnn nnnnnnnngn nnnnnnnnnt nnnnnnnnnn naaaannnnn nnncc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatgcgccc acaacctttg ccgatagtgt taccgttccg gtttcgctgg gggatcgttc ctacgatatt ctgatcggca aggggcttgt cgagcgcgcg ggcgaggaag tcgccaagcg gctgaagggg gttcgtgtcg ccatcgtcac cgatgagaat gtggcggcag tgcatcttga gcgtttgcag gcaagctttg cacgggccgg tatcgattcc acgccggtga ttgtcgcacc tggagaaaaa tccaaatcct ttgcgacgct tgaaaccgtc accaacgcca ttctggtggc aaagctggag cgtggcgatg cggtggtggc gttgggcggt ggcgtggtgg gcgacctttc cggctttgtt gcggggatcg tgcggcgcgg catgaatttc gtgcaaatgc caacctcgct tcttgcacag gtcgattcct ccgttggcgg caagaccggc attaataccg cccatggcaa gaacctcgtt ggtgtcttta accagccgca actcgtgctg gccgatacgc aggtgctcga tacgctgagc ccgcgtgaat tccgtgccgg ttatgcggaa gtcgcaaaat atggcctgat cgaccgcccg gatttcttcg cctggcttga agcgaactgg caggaggttt tctccggcgg tgcggcccgc acaaaggcta ttgccgaatc ctgccgctca aaggctgcgg tcgtggcgcg cgatgagcgt gaaaccggcg accgcgccct gctcaatctc ggtcatacat tcggccatgc gctggaaagc gcaaccggct atgattccag ccgtctcgtt catggcgaag gggtcgcgat cggcatggcg ctggcatatc gcttttcggc ccggatgaac cttgccggta ttgaagcggc agagcgggtg gaagcgcatc tgaaagctgt cggcctgccg gtttcgctgg ccgaggtgcc gggcggcctg ccgcccgcgg aaaagctgat ggattatatc gcgcaggaca agaaggtgac gcgcggtacg ctgaccttca ttctcacgca tggcatcggc cagtccttca tcgcaaagga cgtaccgcct gctgccgtgc tggaatttct gaaagagcgc ctcgccatag cttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa