SSGCID: Target Page

BrabA.17679.a

Leu/Ile/Val-binding protein homolog 6

CENTER ID:	BrabA.17679.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17679.a.A2.GE31720	Mature protein( BrabA.17679.a )		21	390

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17679.a.A2.PS01052	Mature protein( BrabA.17679.a )		21	390

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.2886
RefSeq:	YP_418775.1
UniProt:	Q2YKQ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKIALTALA VFSLAASAAY ADVVKVGVIG PFSGPFALQG KNFKAGIDAY MAEHGNKVGD DTVEVVYRDV PQADPAQSKA LAQELVVKEG VQYLAGFYFT PDAMAVTPIL KQGNVPMVVM NAATSSIVTK SPYVVRTSFT TWQTSTPIAR VALDKGVKKV ISVVSDYGPG VDAENAFKAA FTDAGGEVVE AIRMPLATND FSPIMQRIKD SGAQGVFAFL PSGPTTFGFM KAYVDNGLKS SGIQLFAPGD LTQESDLPAL GENALGVLTT FHYAVSHDSP ENRKFVEEAR KAIGNPAELS FPSVGAYDGM HVIYKMIEAT GGKKDAAKAV EAVKGMEWVS PRGPVSIDPE SRHITQNIYL REVAKADDGT YYNKEIQTFE KQGDPGLKAQ

NT Sequence

ATGAAGAAAA TTGCGCTCAC CGCGCTCGCG GTCTTTTCGC TTGCGGCTTC GGCGGCCTAT GCCGATGTTG TGAAAGTCGG CGTCATAGGT CCGTTTTCGG GGCCGTTTGC CCTGCAAGGC AAGAATTTCA AGGCGGGCAT TGATGCCTAT ATGGCCGAGC ACGGCAACAA GGTGGGCGAC GACACGGTCG AGGTCGTCTA TCGCGACGTG CCGCAGGCCG ACCCCGCACA ATCCAAGGCT CTGGCACAGG AACTCGTGGT GAAGGAAGGC GTGCAATATC TTGCCGGTTT CTACTTCACC CCCGACGCCA TGGCCGTGAC GCCCATCCTG AAGCAGGGCA ATGTGCCGAT GGTGGTGATG AATGCGGCCA CCTCATCCAT CGTGACCAAA AGCCCCTATG TGGTGCGCAC ATCCTTCACC ACCTGGCAGA CCTCGACACC TATTGCCAGG GTCGCACTCG ACAAGGGCGT GAAAAAGGTC ATTTCGGTCG TGAGCGATTA CGGCCCCGGC GTGGACGCCG AAAATGCCTT CAAGGCGGCT TTCACCGATG CCGGTGGCGA AGTGGTGGAA GCCATCCGTA TGCCGCTTGC CACCAATGAT TTCAGCCCCA TCATGCAGCG CATCAAGGAT TCCGGGGCAC AGGGCGTGTT CGCCTTCCTG CCTTCCGGCC CCACCACGTT CGGCTTCATG AAGGCCTATG TCGATAACGG CCTGAAAAGC TCCGGCATCC AGCTTTTCGC TCCCGGTGAT CTGACGCAGG AATCCGACCT GCCCGCGCTT GGCGAAAATG CGCTCGGCGT GCTGACCACG TTCCACTATG CGGTTTCGCA CGATTCACCT GAAAACAGGA AATTCGTTGA GGAAGCCCGC AAGGCCATCG GCAATCCGGC TGAACTCTCC TTCCCGTCGG TCGGCGCTTA TGACGGTATG CATGTCATCT ACAAGATGAT CGAAGCAACT GGCGGCAAGA AAGATGCCGC GAAGGCTGTC GAAGCAGTCA AGGGCATGGA ATGGGTAAGC CCACGCGGCC CGGTTTCCAT CGATCCCGAA AGCCGCCACA TAACGCAGAA TATCTATCTG CGCGAAGTGG CCAAGGCGGA TGACGGCACT TACTACAACA AGGAAATCCA GACCTTCGAG AAGCAGGGCG ATCCGGGCCT GAAAGCGCAG

Details for BrabA.17679.a.A2.GE31720

HARVESTED ON:	2/23/2011
SEQUENCED ON:	3/1/2011
EXPECTED MW:	42kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	50

Validated AA Sequence

MPLATNDFSP IMQRIKDSGA QGVFAFLPSG PTTFGFMKAY VDNGLKSSGI QLFAPGDLTQ ESDLPALGEN ALGVLTTFHY AVSHDSPENR KFVEEARKAI GNPAELSFPS VGAYDGMHVI YKMIEATGGK KDAAKAVEAV KGMEWVSPRG PVSIDPESRH ITQNIYLREV AKADDGTYYN KEIQTFEKQG DPGLKAQ

Validated NT Sequence

ttattactgc gctttcaggc ccggatcgcc ctgcttctcg aaggtctgga tttccttgtt gtagtaagtg ccgtcatccg ccttggccac ttcgcgcaga tagatattct gcgttatgtg gcggctttcg ggatcgatgg aaaccgggcc gcgtgggctt acccattcca tgcccttgac tgcttcgaca gccttcgcgg catctttctt gccgccagtt gcttcgatca tcttgtagat gacatgcata ccgtcataag cgccgaccga cgggaaggag agttcagccg gattgccgat ggccttgcgg gcttcctcaa cgaatttcct gttttcaggt gaatcgtgcg aaaccgcata gtggaacgtg gtcagcacgc cgagcgcatt ttcgccaagc gcgggcaggt cggattcctg cgtcagatca ccgggagcga aaagctggat gccggagctt ttcaggccgt tatcgacata ggccttcatg aagccgaacg tggtggggcc ggaaggcagg aaggcgaaca cgccctgtgc cccggaatcc ttgatgcgct gcatgatggg gctgaaatca ttggtggcaa gcggcatacg gatggcttcc accacttcgc caccggcatc ggtgaaagcc gccttgaagg cattttcggc gtccacgccg gggccgtaat cgctcacgac cgaaatgacc tttttcacgc ccttgtcgag tgcgaccctg gcaataggtg tcgaggtctg ccnnnggtga aggatgtgcg caccacatag gggcttttgg tcacgatgga tgaggtggcc gcattcatca ccaccatcgg cacattgccc tgcttcagga tgggcgtcac ggccatggcg tcgggggtga agtagaaacc ggcaagatat tgcacgcctt ccttcaccac gagttcctgt gccagagcct tggattgtgc ggggtcggcc tgcggcacgt cgcgatagac gacctcgacc gtgtcgtcgc ccaccttgtt gccgtgctcg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMADVVKVGV IGPFSGPFAL QGKNFKAGID AYMAEHGNKV GDDTVEVVYR DVPQADPAQS KALAQELVVK EGVQYLAGFY FTPDAMAVTP ILKQGNVPMV VMNAATSSIV TKSPYVVRTS FTTWQTSTPI ARVALDKGVK KVISVVSDYG PGVDAENAFK AAFTDAGGEV VEAIRMPLAT NDFSPIMQRI KDSGAQGVFA FLPSGPTTFG FMKAYVDNGL KSSGIQLFAP GDLTQESDLP ALGENALGVL TTFHYAVSHD SPENRKFVEE ARKAIGNPAE LSFPSVGAYD GMHVIYKMIE ATGGKKDAAK AVEAVKGMEW VSPRGPVSID PESRHITQNI YLREVAKADD GTYYNKEIQT FEKQGDPGLK AQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgatgtt gtgaaagtcg gcgtcatagg tccgttttcg gggccgtttg ccctgcaagg caagaatttc aaggcgggca ttgatgccta tatggccgag cacggcaaca aggtgggcga cgacacggtc gaggtcgtct atcgcgacgt gccgcaggcc gaccccgcac aatccaaggc tctggcacag gaactcgtgg tgaaggaagg cgtgcaatat cttgccggtt tctacttcac ccccgacgcc atggccgtga cgcccatcct gaagcagggc aatgtgccga tggtggtgat gaatgcggcc acctcatcca tcgtgaccaa aagcccctat gtggtgcgca catccttcac cacctggcag acctcgacac ctattgccag ggtcgcactc gacaagggcg tgaaaaaggt catttcggtc gtgagcgatt acggccccgg cgtggacgcc gaaaatgcct tcaaggcggc tttcaccgat gccggtggcg aagtggtgga agccatccgt atgccgcttg ccaccaatga tttcagcccc atcatgcagc gcatcaagga ttccggggca cagggcgtgt tcgccttcct gccttccggc cccaccacgt tcggcttcat gaaggcctat gtcgataacg gcctgaaaag ctccggcatc cagcttttcg ctcccggtga tctgacgcag gaatccgacc tgcccgcgct tggcgaaaat gcgctcggcg tgctgaccac gttccactat gcggtttcgc acgattcacc tgaaaacagg aaattcgttg aggaagcccg caaggccatc ggcaatccgg ctgaactctc cttcccgtcg gtcggcgctt atgacggtat gcatgtcatc tacaagatga tcgaagcaac tggcggcaag aaagatgccg cgaaggctgt cgaagcagtc aagggcatgg aatgggtaag cccacgcggc ccggtttcca tcgatcccga aagccgccac ataacgcaga atatctatct gcgcgaagtg gccaaggcgg atgacggcac ttactacaac aaggaaatcc agaccttcga gaagcagggc gatccgggcc tgaaagcgca gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for BrabA.17679.a.A2.PS01052

PURIFICATION DATe:	4/19/2011
CONCENTRATION:	58.17mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	50

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGATGTT GTGAAAGTCG GCGTCATAGG TCCGTTTTCG GGGCCGTTTG CCCTGCAAGG CAAGAATTTC AAGGCGGGCA TTGATGCCTA TATGGCCGAG CACGGCAACA AGGTGGGCGA CGACACGGTC GAGGTCGTCT ATCGCGACGT GCCGCAGGCC GACCCCGCAC AATCCAAGGC TCTGGCACAG GAACTCGTGG TGAAGGAAGG CGTGCAATAT CTTGCCGGTT TCTACTTCAC CCCCGACGCC ATGGCCGTGA CGCCCATCCT GAAGCAGGGC AATGTGCCGA TGGTGGTGAT GAATGCGGCC ACCTCATCCA TCGTGACCAA AAGCCCCTAT GTGGTGCGCA CATCCTTCAC CACCTGGCAG ACCTCGACAC CTATTGCCAG GGTCGCACTC GACAAGGGCG TGAAAAAGGT CATTTCGGTC GTGAGCGATT ACGGCCCCGG CGTGGACGCC GAAAATGCCT TCAAGGCGGC TTTCACCGAT GCCGGTGGCG AAGTGGTGGA AGCCATCCGT ATGCCGCTTG CCACCAATGA TTTCAGCCCC ATCATGCAGC GCATCAAGGA TTCCGGGGCA CAGGGCGTGT TCGCCTTCCT GCCTTCCGGC CCCACCACGT TCGGCTTCAT GAAGGCCTAT GTCGATAACG GCCTGAAAAG CTCCGGCATC CAGCTTTTCG CTCCCGGTGA TCTGACGCAG GAATCCGACC TGCCCGCGCT TGGCGAAAAT GCGCTCGGCG TGCTGACCAC GTTCCACTAT GCGGTTTCGC ACGATTCACC TGAAAACAGG AAATTCGTTG AGGAAGCCCG CAAGGCCATC GGCAATCCGG CTGAACTCTC CTTCCCGTCG GTCGGCGCTT ATGACGGTAT GCATGTCATC TACAAGATGA TCGAAGCAAC TGGCGGCAAG AAAGATGCCG CGAAGGCTGT CGAAGCAGTC AAGGGCATGG AATGGGTAAG CCCACGCGGC CCGGTTTCCA TCGATCCCGA AAGCCGCCAC ATAACGCAGA ATATCTATCT GCGCGAAGTG GCCAAGGCGG ATGACGGCAC TTACTACAAC AAGGAAATCC AGACCTTCGA GAAGCAGGGC GATCCGGGCC TGAAAGCGCA GTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA