SSGCID: Target Page

BrabA.17662.a

Flagellar basal-body rod protein FlgF

CENTER ID:	BrabA.17662.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17662.a.A1.GE31718	Full length( BrabA.17662.a )		1	258

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.2405
RefSeq:	YP_418363.1
UniProt:	Q2YLD0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSYRLIASN MTRSLQRVSK EPLVERETAY YKENIGNVKS VDDFMADTRL FNYAMKAFGL EDMAYAKAFV RKILTEGVTD EDAMANKLTD KRYKEFATVF DFAGKGAQAT QSTAAQQGVI DKYVRQTLEK EAGSQNEGVR LALYFERKAP SLTNAYEILG DKALLTMLQT TFGWPSTMSN ADIDKQAKMI ADKIDFSKMS DPDYVSKFIS RFTAMHEMNN PTASSPASIA SFLLGGTSSI GISMNILATL QNFKPGGR

NT Sequence

ATGACGAGCT ATCGCCTGAT CGCGTCGAAT ATGACGCGGT CATTGCAGCG CGTTTCAAAA GAGCCGCTGG TGGAGCGCGA AACGGCCTAT TACAAGGAAA ATATCGGCAA TGTGAAATCG GTTGATGATT TCATGGCCGA TACGCGGCTC TTCAATTACG CCATGAAGGC GTTCGGCCTT GAAGACATGG CCTATGCCAA AGCTTTCGTG CGCAAGATCC TGACTGAAGG CGTCACCGAT GAAGACGCGA TGGCCAATAA GCTGACCGAC AAACGCTACA AGGAATTTGC CACCGTCTTC GATTTTGCAG GCAAAGGCGC GCAGGCCACG CAGAGTACGG CGGCGCAGCA GGGTGTGATT GACAAATATG TCCGCCAGAC GCTGGAAAAG GAAGCGGGCA GCCAGAATGA GGGCGTCCGG CTTGCGCTCT ATTTCGAGCG CAAGGCGCCC TCGCTCACCA ATGCCTATGA AATTCTTGGC GACAAGGCAT TGCTGACCAT GCTGCAGACG ACCTTTGGCT GGCCGTCCAC CATGTCCAAT GCAGATATCG ACAAGCAGGC GAAAATGATC GCCGACAAAA TCGATTTTTC CAAGATGTCC GATCCCGACT ATGTATCGAA GTTCATTTCG CGCTTCACGG CCATGCATGA AATGAACAAT CCGACCGCGA GCAGTCCGGC CTCCATCGCA TCGTTTCTTC TGGGCGGCAC CTCATCCATC GGCATTTCGA TGAACATCCT CGCAACCCTG CAAAACTTCA AGCCGGGTGG GCGC

Details for BrabA.17662.a.A1.GE31718

HARVESTED ON:	2/23/2011
SEQUENCED ON:	3/1/2011
EXPECTED MW:	30kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTSYRLIAS NMTRSLQRVS KEPLVERETA YYKENIGNVK SVDDFMADTR LFNYAMKAFG LEDMAYAKAF VRKILTEGVT DEDAMANKLT DKRYKEFATV FDFAGKGAQA TQSTAAQQGV IDKYVRQTLE KEAGSQNEGV RLALYFERKA PSLTNAYEIL GDKALLTMLQ TTFGWPSTMS NADIDKQAKM IADKIDFSKM SDPDYVSKFI SRFTAMHEMN NPTASSPASI ASFLLGGTSS IGISMNILAT LQNFKPGGR

Validated NT Sequence

ttattagcgc ccacccggct tgaagttttg cagggttgcg aggatgttca tcgaaatgcc gatggatgag gtgccgccca gaagaaacga tgcgatggag gccggactgc tcgcggtcgg attgttcatt tcatgcatgg ccgtgaagcg cgaaatgaac ttcgatacat agtcgggatc ggacatcttg gaaaaatcga ttttgtcggc gatcattttc gcctgcttgt cgatatctgc attggacatg gtggacggcc agccaaaggt cgtctgcagc atggtcagca atgccttgtc gccaagaatt tcataggcat tggtgagcga gggcgccttg cgctcgaaat agagcgcaag ccggacgccc tcattctggc tgcccgcttc cttttccagc gtctggcgga catatttgtc aatcacaccc tgctgcgccg ccgtactctg cgtggcctgc gcgcctttgc ctgcaaaatc gaagacggtg gcaaattcct tgtagcgttt gtcggtcagc ttattggcca tcgcgtcttc atcggtgacg ccttcagtca ggatcttgcg cacgaaagct ttggcatagg ccatgtcttc aaggccgaac gccttcatgg cgtaattgaa gagccgcgta tcggccatga aatcatcaac cgatttcaca ttgccgatat tttccttgta ataggccgtt tcgcgctcca ccagcggctc ttttgaaacg cgctgcaatg accgcgtcat attcgacgcg atcaggcgat agctcgtcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc catggta

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgagctat cgcctgatcg cgtcgaatat gacgcggtca ttgcagcgcg tttcaaaaga gccgctggtg gagcgcgaaa cggcctatta caaggaaaat atcggcaatg tgaaatcggt tgatgatttc atggccgata cgcggctctt caattacgcc atgaaggcgt tcggccttga agacatggcc tatgccaaag ctttcgtgcg caagatcctg actgaaggcg tcaccgatga agacgcgatg gccaataagc tgaccgacaa acgctacaag gaatttgcca ccgtcttcga ttttgcaggc aaaggcgcgc aggccacgca gagtacggcg gcgcagcagg gtgtgattga caaatatgtc cgccagacgc tggaaaagga agcgggcagc cagaatgagg gcgtccggct tgcgctctat ttcgagcgca aggcgccctc gctcaccaat gcctatgaaa ttcttggcga caaggcattg ctgaccatgc tgcagacgac ctttggctgg ccgtccacca tgtccaatgc agatatcgac aagcaggcga aaatgatcgc cgacaaaatc gatttttcca agatgtccga tcccgactat gtatcgaagt tcatttcgcg cttcacggcc atgcatgaaa tgaacaatcc gaccgcgagc agtccggcct ccatcgcatc gtttcttctg ggcggcacct catccatcgg catttcgatg aacatcctcg caaccctgca aaacttcaag ccgggtgggc gctaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa