SSGCID: Target Page

BrabA.17661.c

Glutamate Aspartate periplasmic binding protein precursor GltI (TC 3.A.1.3.4)

CENTER ID:	BrabA.17661.c
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17661.c.B1.GE37965	Full length( BrabA.17661.c )		1	247

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel86222_0797

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDVLIRNTTW TISRDTSLGL DFAGINYYDG QGFMINSKKL AGINSALQLS GASICVQAGT TTELNMADYF RANKMEYNPV VFEKIEEANA AYDSGRCDAY TTDQSSLYGV RLALANPDDH VILPEIISKE PFGLTVRQGD ARWADVVRWT HNALLNAEEY GITQANVEEM KKSDNPDIKR LLGAEADTKI GTDLGLDKDW VVKIIKGVGN YGEIFERNIG SGSPLKIARG LNAQWNKGGL QYGIPVR

NT Sequence

GTGGACGTTC TGATCCGCAA CACGACCTGG ACCATCAGCC GCGATACGTC GCTTGGTCTC GATTTTGCTG GCATCAACTA CTATGACGGC CAGGGCTTCA TGATCAATTC AAAGAAGCTG GCGGGCATCA ATTCCGCATT GCAGCTTTCG GGTGCTTCCA TCTGCGTCCA GGCCGGCACG ACGACCGAGC TGAACATGGC CGACTATTTC CGCGCCAATA AGATGGAATA TAATCCGGTT GTCTTTGAGA AGATCGAAGA AGCCAATGCC GCTTATGATT CCGGCCGTTG TGACGCCTAT ACGACCGACC AGTCCAGCCT TTACGGGGTT CGTCTGGCGC TTGCCAATCC GGACGATCAC GTCATCCTGC CGGAAATCAT TTCCAAGGAG CCTTTCGGCC TGACCGTTCG TCAAGGTGAC GCCAGGTGGG CGGATGTCGT TCGCTGGACG CATAATGCGC TGCTCAATGC CGAGGAATAC GGCATTACGC AGGCCAATGT CGAAGAGATG AAGAAGTCCG ATAATCCGGA TATCAAGCGT CTTCTCGGCG CGGAAGCAGA CACCAAGATC GGCACCGATC TCGGCCTCGA CAAGGACTGG GTGGTGAAGA TCATCAAGGG TGTCGGCAAT TACGGCGAAA TCTTCGAGCG CAACATTGGT TCGGGCAGCC CGCTCAAGAT CGCACGCGGC CTCAACGCCC AGTGGAACAA GGGCGGCCTG CAATATGGCA TCCCCGTGCG C

Details for BrabA.17661.c.B1.GE37965

HARVESTED ON:	5/12/2014
SEQUENCED ON:	5/4/2014
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Validated AA Sequence

MVDVLIRNTT WTISRDTSLG LDFAGINYYD GQGFMINSKK LAGINSALQL SGASICVQAG TTTELNMADY FRANKMEYNP VVFEKIEEAN AAYDSGRCDA YTTDQSSLYG VRLALANPDD HVILPEIISK EPFGLTVRQG DARWADVVRW THNALLNAEE YGITQANVEE MKKSDNPDIK RLLGAEADTK IGTDLGLDKD WVVKIIKGVG NYGEIFERNI GSGSPLKIAR GLNAQWNKRG LQYGIPVR

Validated NT Sequence

ctagaatant tttnnttaac tttaagaagg aganatacca tggcttcacc accaccacca ccatatggtg gacgttctga tccgcaacac gacctggacc atcagccgcg atacgtcgct nggtctcgat tttgctggca tcaactacta tgacggccag ggcttcatga tcaattcaaa gaagctggcg ggcatcaatt ccgcattgca gctttcgggt gcttccatct gcgtccaggc cggcacgacg accgagctga acatggccga ctatttccgc gccaataaga tggaatataa tccggttgtc tttgagaaga tcgaagaagc caatgccgct tatgattccg gccgttgtga cgcctatacg accgaccagt ccagccttta cggggttcgt ctggcgcttg ccaatccgga cgatcacgtc atcctgccgg aaatcatttc caaggagcct ttcggcctga ccgttcgtca aggtgacgcc aggtgggcgg atgtcgttcg ctggacgcat aatgcgctgc tcaatgccga ggaatacggc attacgcagg ccaatgtcga agagatgaag aagtccgata atccggatat caagcgtctt ctcggcgcgg aagcagacac caagatcggc accgatctcg gcctcgacaa ggactgggtg gtgaagatca tcaagggtgt cggcaattac ggcgaaatct tcgagcgcaa cattggttcg ggcagcccgc tcaagatcgc acgcggcctc aacgcccagt ggaacaagcg cggcctgcaa tatggcatcc ccgtgcgcta agtgagtaag ataggatccg tgnnnncnnn nagnnnnggg aaanannnnn anaancncan ttccngcgca taattcaggc ggtgggangg cagngntacc ncttcatgng atgcggnana agccaagctg tnnntggtgc ggcgtgagcg gtcaggnact ngtngcgctn aaaancccca taaagngtcn ggggnggagg cncnttgcgc tcagcncntt gcnagtanga aantaaaaag gaacnnnnag ntgaagnnnn tgntcgnc

Expected Protein Sequence

MAHHHHHHMD VLIRNTTWTI SRDTSLGLDF AGINYYDGQG FMINSKKLAG INSALQLSGA SICVQAGTTT ELNMADYFRA NKMEYNPVVF EKIEEANAAY DSGRCDAYTT DQSSLYGVRL ALANPDDHVI LPEIISKEPF GLTVRQGDAR WADVVRWTHN ALLNAEEYGI TQANVEEMKK SDNPDIKRLL GAEADTKIGT DLGLDKDWVV KIIKGVGNYG EIFERNIGSG SPLKIARGLN AQWNKGGLQY GIPVR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggacg ttctgatccg caacacgacc tggaccatca gccgcgatac gtcgcttggt ctcgattttg ctggcatcaa ctactatgac ggccagggct tcatgatcaa ttcaaagaag ctggcgggca tcaattccgc attgcagctt tcgggtgctt ccatctgcgt ccaggccggc acgacgaccg agctgaacat ggccgactat ttccgcgcca ataagatgga atataatccg gttgtctttg agaagatcga agaagccaat gccgcttatg attccggccg ttgtgacgcc tatacgaccg accagtccag cctttacggg gttcgtctgg cgcttgccaa tccggacgat cacgtcatcc tgccggaaat catttccaag gagcctttcg gcctgaccgt tcgtcaaggt gacgccaggt gggcggatgt cgttcgctgg acgcataatg cgctgctcaa tgccgaggaa tacggcatta cgcaggccaa tgtcgaagag atgaagaagt ccgataatcc ggatatcaag cgtcttctcg gcgcggaagc agacaccaag atcggcaccg atctcggcct cgacaaggac tgggtggtga agatcatcaa gggtgtcggc aattacggcg aaatcttcga gcgcaacatt ggttcgggca gcccgctcaa gatcgcacgc ggcctcaacg cccagtggaa caagggcggc ctgcaatatg gcatccccgt gcgctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat