BrabA.17661.a

Solute-binding protein/glutamate receptor:Bacterial extracellular solute-binding protein, family 3

CENTER ID: BrabA.17661.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17661.a.A2.GE34635 Mature protein( BrabA.17661.a ) 24 339
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.2576
RefSeq: YP_418499.1
UniProt: Q2YLB5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKTVWGALL AAGFALSAQG GAAADTLSTV KERGKVNCGV SQGVAGFSSP DDQGKWTGFD IDFCRAVSAA VFGDPDKVSF IPLSTKERFT ALQSGTVDLL SCQTTWTLSR DAGMGIHFVG TAYYDGQGFM IRKDLGVDSA LKLDGASICT EQGTTTEQNV ADYCGAHNIK YEPVVIDSAD GILKAFETGR CDVYTTDASA LYAQRLKLAE PDAYMVLPEV ISKEPLGPAV RQGDDKWFNI VRWTLFALLE AEELGITQKT VAADLDSRKP DIRRFLGSEG DNGQQLGLEP KWAYNIVASV GNYGEMFERT IGEGSLLKIN RGINALWNKG GLMYAPPVK
NT Sequence
ATGAAAAAGA CAGTTTGGGG TGCGTTGCTG GCGGCGGGCT TTGCCCTGTC GGCACAAGGC GGGGCTGCGG CGGACACGCT TTCGACGGTC AAGGAACGCG GCAAGGTCAA CTGCGGCGTC AGCCAGGGCG TTGCGGGCTT TTCGTCGCCG GACGATCAGG GCAAATGGAC CGGCTTCGAC ATTGATTTCT GCCGTGCGGT TTCGGCTGCG GTCTTTGGCG ATCCCGACAA GGTGAGCTTC ATTCCGCTTT CGACCAAGGA ACGTTTTACC GCATTGCAAT CGGGGACGGT CGATCTGCTT TCGTGCCAGA CCACCTGGAC GCTTTCGCGC GATGCAGGCA TGGGCATCCA TTTTGTCGGC ACCGCCTATT ATGACGGACA GGGCTTCATG ATCCGCAAGG ATCTGGGTGT GGACAGCGCC CTGAAGCTTG ACGGTGCTTC CATCTGCACC GAACAGGGCA CGACGACCGA ACAGAACGTG GCCGATTATT GCGGCGCCCA TAATATCAAA TATGAGCCGG TGGTGATCGA TTCCGCTGAC GGTATTCTGA AAGCATTCGA GACGGGGCGT TGCGACGTCT ATACGACCGA CGCTTCGGCG CTTTATGCGC AGCGCCTCAA GCTTGCCGAG CCGGACGCCT ATATGGTGTT GCCGGAGGTT ATTTCAAAAG AGCCGCTTGG CCCCGCTGTT CGCCAGGGGG ACGATAAATG GTTCAATATT GTGCGCTGGA CGCTGTTTGC CTTGCTGGAA GCGGAAGAGC TCGGCATCAC ACAGAAGACG GTGGCAGCGG ACCTGGATTC CCGGAAGCCG GATATACGCC GCTTCCTCGG CTCGGAAGGC GATAATGGCC AGCAACTGGG CCTCGAACCG AAATGGGCTT ATAATATCGT GGCGTCGGTC GGCAATTATG GCGAGATGTT CGAGCGCACC ATCGGTGAGG GCAGCCTGCT CAAGATCAAC CGGGGTATCA ATGCGCTCTG GAACAAGGGT GGGCTGATGT ACGCCCCGCC GGTCAAA
Details for BrabA.17661.a.A2.GE34635
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/16/2012
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMADTLSTVK ERGKVNCGVS QGVAGFSSPD DQGKWTGFDI DFCRAVSAAV FGDPDKVSFI PLSTKERFTA LQSGTVDLLS CQTTWTLSRD AGMGIHFVGT AYYDGQGFMI RKDLGVDSAL KLDGASICTE QGTTTEQNVA DYCGAHNIKY EPVVIDSADG ILKAFETGRC DVYTTDASAL YAQRLKLAEP DAYMVLPEVI SKEPLGPAVR QGDDKWFNIV RWTLFALLEA EELGITQKTV AADLDSRKPD IRRFLGSEGD NGQQLGLEPK WAYNIVASVG NYGEMFERTI GEGSLLKINR GINALWNKGG LMYAPPVK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgg acacgctttc gacggtcaag gaacgcggca aggtcaactg cggcgtcagc cagggcgttg cgggcttttc gtcgccggac gatcagggca aatggaccgg cttcgacatt gatttctgcc gtgcggtttc ggctgcggtc tttggcgatc ccgacaaggt gagcttcatt ccgctttcga ccaaggaacg ttttaccgca ttgcaatcgg ggacggtcga tctgctttcg tgccagacca cctggacgct ttcgcgcgat gcaggcatgg gcatccattt tgtcggcacc gcctattatg acggacaggg cttcatgatc cgcaaggatc tgggtgtgga cagcgccctg aagcttgacg gtgcttccat ctgcaccgaa cagggcacga cgaccgaaca gaacgtggcc gattattgcg gcgcccataa tatcaaatat gagccggtgg tgatcgattc cgctgacggt attctgaaag cattcgagac ggggcgttgc gacgtctata cgaccgacgc ttcggcgctt tatgcgcagc gcctcaagct tgccgagccg gacgcctata tggtgttgcc ggaggttatt tcaaaagagc cgcttggccc cgctgttcgc cagggggacg ataaatggtt caatattgtg cgctggacgc tgtttgcctt gctggaagcg gaagagctcg gcatcacaca gaagacggtg gcagcggacc tggattcccg gaagccggat atacgccgct tcctcggctc ggaaggcgat aatggccagc aactgggcct cgaaccgaaa tgggcttata atatcgtggc gtcggtcggc aattatggcg agatgttcga gcgcaccatc ggtgagggca gcctgctcaa gatcaaccgg ggtatcaatg cgctctggaa caagggtggg ctgatgtacg ccccgccggt caaataataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADTLSTVK ERGKVNCGVS QGVAGFSSPD DQGKWTGFDI DFCRAVSAAV FGDPDKVSFI PLSTKERFTA LQSGTVDLLS CQTTWTLSRD AGMGIHFVGT AYYDGQGFMI RKDLGVDSAL KLDGASICTE QGTTTEQNVA DYCGAHNIKY EPVVIDSADG ILKAFETGRC DVYTTDASAL YAQRLKLAEP DAYMVLPEVI SKEPLGPAVR QGDDKWFNIV RWTLFALLEA EELGITQKTV AADLDSRKPD IRRFLGSEGD NGQQLGLEPK WAYNIVASVG NYGEMFERTI GEGSLLKINR GINALWNKGG LMYAPPVK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggacacg ctttcgacgg tcaaggaacg cggcaaggtc aactgcggcg tcagccaggg cgttgcgggc ttttcgtcgc cggacgatca gggcaaatgg accggcttcg acattgattt ctgccgtgcg gtttcggctg cggtctttgg cgatcccgac aaggtgagct tcattccgct ttcgaccaag gaacgtttta ccgcattgca atcggggacg gtcgatctgc tttcgtgcca gaccacctgg acgctttcgc gcgatgcagg catgggcatc cattttgtcg gcaccgccta ttatgacgga cagggcttca tgatccgcaa ggatctgggt gtggacagcg ccctgaagct tgacggtgct tccatctgca ccgaacaggg cacgacgacc gaacagaacg tggccgatta ttgcggcgcc cataatatca aatatgagcc ggtggtgatc gattccgctg acggtattct gaaagcattc gagacggggc gttgcgacgt ctatacgacc gacgcttcgg cgctttatgc gcagcgcctc aagcttgccg agccggacgc ctatatggtg ttgccggagg ttatttcaaa agagccgctt ggccccgctg ttcgccaggg ggacgataaa tggttcaata ttgtgcgctg gacgctgttt gccttgctgg aagcggaaga gctcggcatc acacagaaga cggtggcagc ggacctggat tcccggaagc cggatatacg ccgcttcctc ggctcggaag gcgataatgg ccagcaactg ggcctcgaac cgaaatgggc ttataatatc gtggcgtcgg tcggcaatta tggcgagatg ttcgagcgca ccatcggtga gggcagcctg ctcaagatca accggggtat caatgcgctc tggaacaagg gtgggctgat gtacgccccg ccggtcaaat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa