BrabA.17646.a

Low-specificity threonine aldolase

CENTER ID: BrabA.17646.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17646.a.A1.GE34637 Full length( BrabA.17646.a ) 1 349
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.2328
RefSeq: YP_418296.1
UniProt: Q2YJ80

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHFASDNWAG AHPKIAESLS LHAAGFAAAY GASEIDKRVE QHFNELFERE VAVFFVGTGT AANSLALASV NRPGGVSLVH REAHVIEDGC GAPEYFTGGA RLHPIDGALG RIDPELLKRE LKRFNPAFVH AGQPMAVSIT QATEVGTLYQ PEHIATISQI CRDAGLPLHM DGARFANALV SLDLTPAEMT WKLGVDIVSF GGTKNGCWCA EALVFMDPAR AKDLPFIRKR AAQLFSKTRF VAAQFDAYLR DDLWRDLARH SNALAARLAN HIHASSQMRL AWKPEANEVF AIMKQSVYDR LRDAGAVFYD WNPPHSEVGR IGEGEIFARF VTSFANTEEE VDRFGDMIA
NT Sequence
GTGCATTTTG CGTCAGACAA TTGGGCAGGC GCCCATCCAA AAATCGCCGA GAGCCTTTCG CTGCATGCAG CCGGTTTTGC GGCAGCCTAT GGCGCAAGCG AGATCGACAA ACGTGTGGAA CAACACTTCA ATGAATTGTT CGAGCGTGAA GTGGCCGTTT TCTTTGTCGG AACCGGTACG GCTGCCAATT CGCTGGCGCT GGCCAGCGTC AACCGGCCCG GCGGCGTTTC GCTGGTCCAC CGCGAGGCGC ATGTGATCGA GGATGGATGC GGCGCACCGG AATATTTCAC CGGCGGCGCA CGTCTCCACC CGATCGACGG CGCGCTGGGC CGCATTGATC CCGAACTTCT GAAACGCGAA TTGAAGCGTT TCAACCCCGC CTTCGTTCAC GCGGGCCAGC CCATGGCCGT CAGCATCACG CAGGCGACGG AAGTGGGTAC GCTTTACCAG CCGGAGCATA TCGCCACCAT TTCGCAAATC TGCCGCGATG CCGGACTGCC GCTACACATG GATGGCGCAC GCTTTGCCAA TGCGCTCGTG TCGCTTGATC TCACACCTGC CGAAATGACA TGGAAACTTG GCGTGGACAT TGTGTCCTTC GGCGGCACCA AGAATGGCTG CTGGTGCGCC GAGGCGCTGG TCTTCATGGA CCCGGCCCGC GCCAAAGACC TGCCCTTCAT CCGCAAGCGC GCAGCACAGC TTTTCTCCAA GACGCGCTTC GTTGCCGCGC AGTTCGATGC CTATCTGCGT GACGATCTCT GGCGCGACCT TGCCCGCCAT TCCAACGCGC TTGCTGCCCG CCTTGCCAAC CATATCCATG CATCAAGCCA GATGCGCCTT GCCTGGAAAC CGGAAGCCAA CGAGGTTTTC GCGATCATGA AGCAATCGGT TTATGACCGT CTGCGCGATG CGGGTGCGGT ATTCTACGAC TGGAATCCTC CGCATTCGGA AGTGGGCCGC ATCGGCGAGG GCGAGATTTT TGCCCGCTTT GTCACCAGCT TCGCCAATAC GGAAGAAGAA GTGGACCGTT TCGGCGACAT GATCGCC
Details for BrabA.17646.a.A1.GE34637
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/16/2012
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVHFASDNW AGAHPKIAES LSLHAAGFAA AYGASEIDKR VEQHFNELFE REVAVFFVGT GTAANSLALA SVNRPGGVSL VHREAHVIED GCGAPEYFTG GARLHPIDGA LGRIDPELLK RELKRFNPAF VHAGQPMAVS ITQATEVGTL YQPEHIATIS QICRDAGLPL HMDGARFANA LVSLDLTPAE MTWKLGVDIV SFGGTKNGCW CAEALVFMDP ARAKDLPFIR KRAAQLFSKT RFVAAQFDAY LRDDLWRDLA RHSNALAARL ANHIHASSQM RLAWKPEANE VFAIMKQSVY DRLRDAGAVF YDWNPPHSEV GRIGEGEIFA RFVTSFANTE EEVDRFGDMI
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgc attttgcgtc agacaattgg gcaggcgccc atccaaaaat cgccgagagc ctttcgctgc atgcagccgg ttttgcggca gcctatggcg caagcgagat cgacaaacgt gtggaacaac acttcaatga attgttcgag cgtgaagtgg ccgttttctt tgtcggaacc ggtacggctg ccaattcgct ggcgctggcc agcgtcaacc ggcccggcgg cgtttcgctg gtccaccgcg aggcgcatgt gatcgaggat ggatgcggcg caccggaata tttcaccggc ggcgcacgtc tccacccgat cgacggcgcg ctgggccgca ttgatcccga acttctgaaa cgcgaattga agcgtttcaa ccccgccttc gttcacgcgg gccagcccat ggccgtcagc atcacgcagg cgacggaagt gggtacgctt taccagccgg agcatatcgc caccatttcg caaatctgcc gcgatgccgg actgccgcta cacatggatg gcgcacgctt tgccaatgcg ctcgtgtcgc ttgacctcac acctgccgaa atgacatgga aacttggcgt ggacattgtg tccttcggcg gcaccaagaa tggctgctgg tgcgccgagg cgctggtctt catggacccg gcccgcgcca aagacctgcc cttcatccgc aagcgcgcag cacagctttt ctccaagacg cgcttcgttg ccgcgcagtt cgatgcctat ctgcgtgacg atctctggcg cgaccttgcc cgccattcca acgcgcttgc tgcccgcctt gccaaccata tccatgcatc aagccagatg cgccttgcct ggaaaccgga agccaacgag gttttcgcga tcatgaagca atcggtttat gaccgtctgc gcgatgcggg tgcggtattc tacgactgga atcctccgca ttcggaagtg ggccgcatcg gcgagggcga gatttttgcc cgctttgtca ccagcttcgc caatacggaa gaagaagtgg accgtttcgg cgacatgatc g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVHFASDNW AGAHPKIAES LSLHAAGFAA AYGASEIDKR VEQHFNELFE REVAVFFVGT GTAANSLALA SVNRPGGVSL VHREAHVIED GCGAPEYFTG GARLHPIDGA LGRIDPELLK RELKRFNPAF VHAGQPMAVS ITQATEVGTL YQPEHIATIS QICRDAGLPL HMDGARFANA LVSLDLTPAE MTWKLGVDIV SFGGTKNGCW CAEALVFMDP ARAKDLPFIR KRAAQLFSKT RFVAAQFDAY LRDDLWRDLA RHSNALAARL ANHIHASSQM RLAWKPEANE VFAIMKQSVY DRLRDAGAVF YDWNPPHSEV GRIGEGEIFA RFVTSFANTE EEVDRFGDMI A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcatttt gcgtcagaca attgggcagg cgcccatcca aaaatcgccg agagcctttc gctgcatgca gccggttttg cggcagccta tggcgcaagc gagatcgaca aacgtgtgga acaacacttc aatgaattgt tcgagcgtga agtggccgtt ttctttgtcg gaaccggtac ggctgccaat tcgctggcgc tggccagcgt caaccggccc ggcggcgttt cgctggtcca ccgcgaggcg catgtgatcg aggatggatg cggcgcaccg gaatatttca ccggcggcgc acgtctccac ccgatcgacg gcgcgctggg ccgcattgat cccgaacttc tgaaacgcga attgaagcgt ttcaaccccg ccttcgttca cgcgggccag cccatggccg tcagcatcac gcaggcgacg gaagtgggta cgctttacca gccggagcat atcgccacca tttcgcaaat ctgccgcgat gccggactgc cgctacacat ggatggcgca cgctttgcca atgcgctcgt gtcgcttgat ctcacacctg ccgaaatgac atggaaactt ggcgtggaca ttgtgtcctt cggcggcacc aagaatggct gctggtgcgc cgaggcgctg gtcttcatgg acccggcccg cgccaaagac ctgcccttca tccgcaagcg cgcagcacag cttttctcca agacgcgctt cgttgccgcg cagttcgatg cctatctgcg tgacgatctc tggcgcgacc ttgcccgcca ttccaacgcg cttgctgccc gccttgccaa ccatatccat gcatcaagcc agatgcgcct tgcctggaaa ccggaagcca acgaggtttt cgcgatcatg aagcaatcgg tttatgaccg tctgcgcgat gcgggtgcgg tattctacga ctggaatcct ccgcattcgg aagtgggccg catcggcgag ggcgagattt ttgcccgctt tgtcaccagc ttcgccaata cggaagaaga agtggaccgt ttcggcgaca tgatcgccta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a