BrabA.17635.a

Protease, putative

CENTER ID: BrabA.17635.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17635.a.A1.GE34634 Full length( BrabA.17635.a ) 1 310
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.3204
RefSeq: YP_419059.1
UniProt: Q2YJX5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATNATPSLS MGPVLFLWDG PKWRDFYFRI ADEAPVSHVT VGEIVCSKRF HFVDPYMEAV IERLQQAGKT VSLGSLALVM LEREAKQVRQ TIEKATLPVE ANDLSALGLL SGKPHTVGPL VNVYNAATAK VLAERGATSI CLPPELPLAS IREIVKGAPD IAFEVFSFGR VPLAISARCA HARSKGHIKD NCQFVCGEEP DGLPVKTLDS QPFLALNGVQ TLSHTCQAFL EDMPELKAAG VSRFRLSPQV CDMVAVAQIH EDVLLEKIDP DEGVARLKEV YPAVPLSNGF LHGGIGAAWV SRQRSMAAGH
NT Sequence
ATGGCAACGA ATGCGACCCC CAGCCTCAGC ATGGGGCCTG TTCTTTTTCT GTGGGATGGG CCGAAATGGC GGGATTTCTA TTTCCGCATC GCCGACGAAG CGCCGGTAAG CCACGTCACC GTTGGCGAAA TCGTCTGTTC CAAACGGTTT CATTTCGTCG ATCCCTATAT GGAAGCGGTC ATTGAGCGCC TGCAACAGGC GGGCAAGACG GTGAGCCTCG GCTCGCTTGC CCTTGTCATG CTGGAGCGCG AGGCAAAACA GGTGCGCCAG ACAATCGAGA AGGCCACGCT GCCCGTCGAA GCAAACGATC TTTCCGCGCT GGGGCTTCTT TCCGGCAAGC CGCACACGGT CGGCCCGCTC GTCAATGTCT ATAATGCAGC CACGGCCAAG GTTCTGGCCG AGCGCGGCGC AACGTCCATC TGCCTGCCGC CGGAATTGCC GCTCGCTTCA ATCCGCGAGA TCGTCAAGGG TGCGCCGGAC ATCGCCTTCG AGGTCTTCAG CTTCGGGCGC GTGCCGCTTG CCATCTCCGC GCGCTGTGCC CATGCCCGCT CCAAGGGGCA TATCAAGGAC AATTGCCAGT TCGTCTGCGG CGAGGAGCCG GACGGCCTGC CGGTCAAGAC GCTGGACAGC CAGCCATTCC TTGCACTGAA CGGTGTGCAG ACCCTGTCCC ATACATGTCA GGCTTTTCTG GAAGACATGC CGGAACTGAA AGCCGCAGGC GTCAGCCGTT TCCGCCTTTC GCCGCAGGTT TGCGACATGG TGGCTGTCGC GCAAATTCAT GAAGACGTGC TTCTGGAAAA GATCGACCCC GATGAAGGCG TCGCACGGCT CAAGGAAGTC TATCCCGCTG TACCGCTCTC CAACGGGTTT CTGCATGGCG GCATCGGCGC GGCATGGGTC AGCCGTCAGC GCAGCATGGC AGCCGGGCAT
Details for BrabA.17635.a.A1.GE34634
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/16/2012
EXPECTED MW: 35kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMATNATPSL SMGPVLFLWD GPKWRDFYFR IADEAPVSHV TVGEIVCSKR FHFVDPYMEA VIERLQQAGK TVSLGSLALV MLEREAKQVR QTIEKATLPV EANDLSALGL LSGKPHTVGP LVNVYNAATA KVLAERGATS ICLPPELPLA SIREIVNGAP DIAFEVFSFG RVPLAISARC AHARSKGHIK DNCQFVCGEE PDGLPVKTLD SQPFLALNGV QTLSHTCQAF LEDMPELKAA GVSRFRLSPQ VCDMVAVAQI HEDVLLEKID PDEGVARLKE VYPAVPLSNG FLHGGIGAAW VSRQRSMAAG H
Validated NT Sequence
ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggcaacg aatgcgaccc ccagcctcag catggggcct gttctttttc tgtgggatgg gccgaaatgg cgggatttct atttccgcat cgccgacgaa gcgccggtaa gccacgtcac cgttggcgaa atcgtctgtt ccaaacggtt tcatttcgtc gatccctata tggaagcggt cattgagcgc ctgcaacagg cgggcaagac ggtgagcctc ggctcgcttg cccttgtcat gctggagcgc gaggcaaaac aggtgcgcca gacaatcgag aaggccacgc tgcccgtcga agcaaacgat ctttccgcgc tggggcttct ttccggcaag ccgcacacgg tcggcccgct cgtcaatgtc tataatgcag ccacggccaa ggttctggcc gagcgcggcg caacgtccat ctgcctgccg ccggaattgc cgctcgcttc aatccgcgag atcgtcaatg gtgcgccgga catcgccttc gaggtcttca gcttcgggcg cgtgccgctt gccatctccg cgcgctgtgc ccatgcccgc tccaaggggc atatcaagga caattgccag ttcgtctgcg gcgaggagcc ggacggcctg ccggtcaaga cgctggacag ccagccattc cttgcactga acggtgtgca gaccctgtcc catacatgtc aggcttttct ggaagacatg ccggaactga aagccgcagg cgtcagccgt ttccgccttt cgccgcaggt ttgcgacatg gtggctgtcg cgcaaattca tgaagacgtg cttctggaaa agatcgaccc cgatgaaggc gtcgcacggc tcaaggaagt ctatcccgct gtaccgctct ccaacgggtt tctgcatggc ggcatcggcg cggcatgggt cagccgtcag cgcagcatgg cagccgggca ttaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMATNATPSL SMGPVLFLWD GPKWRDFYFR IADEAPVSHV TVGEIVCSKR FHFVDPYMEA VIERLQQAGK TVSLGSLALV MLEREAKQVR QTIEKATLPV EANDLSALGL LSGKPHTVGP LVNVYNAATA KVLAERGATS ICLPPELPLA SIREIVKGAP DIAFEVFSFG RVPLAISARC AHARSKGHIK DNCQFVCGEE PDGLPVKTLD SQPFLALNGV QTLSHTCQAF LEDMPELKAA GVSRFRLSPQ VCDMVAVAQI HEDVLLEKID PDEGVARLKE VYPAVPLSNG FLHGGIGAAW VSRQRSMAAG H
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaacgaat gcgaccccca gcctcagcat ggggcctgtt ctttttctgt gggatgggcc gaaatggcgg gatttctatt tccgcatcgc cgacgaagcg ccggtaagcc acgtcaccgt tggcgaaatc gtctgttcca aacggtttca tttcgtcgat ccctatatgg aagcggtcat tgagcgcctg caacaggcgg gcaagacggt gagcctcggc tcgcttgccc ttgtcatgct ggagcgcgag gcaaaacagg tgcgccagac aatcgagaag gccacgctgc ccgtcgaagc aaacgatctt tccgcgctgg ggcttctttc cggcaagccg cacacggtcg gcccgctcgt caatgtctat aatgcagcca cggccaaggt tctggccgag cgcggcgcaa cgtccatctg cctgccgccg gaattgccgc tcgcttcaat ccgcgagatc gtcaagggtg cgccggacat cgccttcgag gtcttcagct tcgggcgcgt gccgcttgcc atctccgcgc gctgtgccca tgcccgctcc aaggggcata tcaaggacaa ttgccagttc gtctgcggcg aggagccgga cggcctgccg gtcaagacgc tggacagcca gccattcctt gcactgaacg gtgtgcagac cctgtcccat acatgtcagg cttttctgga agacatgccg gaactgaaag ccgcaggcgt cagccgtttc cgcctttcgc cgcaggtttg cgacatggtg gctgtcgcgc aaattcatga agacgtgctt ctggaaaaga tcgaccccga tgaaggcgtc gcacggctca aggaagtcta tcccgctgta ccgctctcca acgggtttct gcatggcggc atcggcgcgg catgggtcag ccgtcagcgc agcatggcag ccgggcatta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a