BrabA.17549.a

7-cyano-7-deazaguanine synthase

CENTER ID: BrabA.17549.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17549.a.A2.GE34629 Mature protein( BrabA.17549.a ) 17 232
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.2055
RefSeq: YP_415306.1
UniProt: Q2YR96

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTLVICSGG LDSVSLAHKM AAEHELTGLL SFDYGQRHKK ELDFAQACAK RLGVPHQIID IRTIGASLTG SALTDDVDVP DGHYAEETMK VTVVPNRNAI MLAIAFGVAA AQKADAVALA VHGGDHFIYP DCRPGFIEAF QTMQKHALDG YADVKLLAPY VHATKADIVA DGAKYRTPFE ATWSCYKGAD RHCGRCGTCV ERREAFHLAG IDDPTSYEDA DFWRATTQKR NA
NT Sequence
ATGAAAACGC TCGTCATCTG CTCCGGCGGA TTGGACTCCG TTTCGCTTGC CCACAAAATG GCGGCGGAAC ATGAACTCAC AGGCCTGCTC TCTTTCGATT ACGGCCAGCG CCACAAGAAG GAGCTGGACT TCGCCCAGGC CTGCGCAAAA AGGCTTGGCG TCCCGCATCA GATCATCGAT ATCCGCACCA TTGGCGCAAG CCTGACCGGC TCGGCGCTCA CCGATGACGT CGATGTGCCG GACGGCCACT ACGCTGAAGA AACGATGAAA GTCACGGTCG TTCCGAATCG CAACGCCATC ATGCTTGCCA TCGCCTTCGG CGTCGCAGCA GCACAGAAAG CCGATGCGGT GGCGCTTGCC GTTCATGGCG GCGATCATTT CATTTATCCC GATTGCCGCC CCGGCTTCAT CGAAGCTTTC CAGACCATGC AGAAACACGC ACTGGACGGC TATGCCGATG TGAAACTGCT TGCTCCTTAC GTCCATGCCA CCAAGGCCGA CATCGTTGCC GATGGCGCGA AATATCGCAC GCCCTTTGAG GCCACATGGT CCTGCTACAA GGGCGCAGAT CGGCATTGCG GGCGCTGCGG CACCTGCGTG GAGCGGCGCG AGGCGTTTCA TCTTGCAGGA ATAGACGACC CGACCAGCTA TGAGGATGCC GACTTCTGGC GCGCCACCAC GCAAAAAAGG AACGCC
Details for BrabA.17549.a.A2.GE34629
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/16/2012
EXPECTED MW: 26kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAHKMAAEH ELTGLLSFDY GQRHKKELDF AQACAKRLGV PHQIIDIRTI GASLTGSALT DDVDVPDGHY AEETMKVTVV PNRNAIMLAI AFGVAAAQKA DAVALAVHGG DHFIYPDCRP GFIEAFQTMQ KHALDGYADV KLLAPYVHAT KADIVADGAK YRTPFEATWS CYKGADRHCG RCGTCVERRE AFHLAGIDDP TSYEDADFWR ATTQKR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggccc acaaaatggc ggcggaacat gaactcacag gcctgctctc tttcgattac ggccagcgcc acaagaagga gctggacttc gcccaggcct gcgcaaaaag gcttggcgtc ccgcatcaga tcatcgatat ccgcaccatt ggcgcaagcc tgaccggctc ggcgctcacc gatgacgtcg atgtgccgga cggccactac gctgaagaaa cgatgaaagt cacggtcgtt ccgaatcgca acgccatcat gcttgccatc gccttcggcg tcgcagcagc acagaaagcc gatgcggtgg cgcttgccgt tcatggcggc gatcatttca tttatcccga ttgccgcccc ggcttcatcg aagctttcca gaccatgcag aaacacgcac tggacggcta tgccgatgtg aaactgcttg ctccttacgt ccatgccacc aaggccgaca tcgttgccga tggcgcgaaa tatcgcacgc cctttgaggc cacatggtcc tgctacaagg gcgcagatcg gcattgcggg cgctgcggca cctgcgtgga gcggcgcgag gcgtttcatc ttgcaggaat agacgacccg accagctatg aggatgccga cttctggcgc gccaccacgc aaaaaagga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAHKMAAEH ELTGLLSFDY GQRHKKELDF AQACAKRLGV PHQIIDIRTI GASLTGSALT DDVDVPDGHY AEETMKVTVV PNRNAIMLAI AFGVAAAQKA DAVALAVHGG DHFIYPDCRP GFIEAFQTMQ KHALDGYADV KLLAPYVHAT KADIVADGAK YRTPFEATWS CYKGADRHCG RCGTCVERRE AFHLAGIDDP TSYEDADFWR ATTQKRNA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcccacaaa atggcggcgg aacatgaact cacaggcctg ctctctttcg attacggcca gcgccacaag aaggagctgg acttcgccca ggcctgcgca aaaaggcttg gcgtcccgca tcagatcatc gatatccgca ccattggcgc aagcctgacc ggctcggcgc tcaccgatga cgtcgatgtg ccggacggcc actacgctga agaaacgatg aaagtcacgg tcgttccgaa tcgcaacgcc atcatgcttg ccatcgcctt cggcgtcgca gcagcacaga aagccgatgc ggtggcgctt gccgttcatg gcggcgatca tttcatttat cccgattgcc gccccggctt catcgaagct ttccagacca tgcagaaaca cgcactggac ggctatgccg atgtgaaact gcttgctcct tacgtccatg ccaccaaggc cgacatcgtt gccgatggcg cgaaatatcg cacgcccttt gaggccacat ggtcctgcta caagggcgca gatcggcatt gcgggcgctg cggcacctgc gtggagcggc gcgaggcgtt tcatcttgca ggaatagacg acccgaccag ctatgaggat gccgacttct ggcgcgccac cacgcaaaaa aggaacgcct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa