SSGCID: Target Page

BrabA.17534.a

Flageller protein FlgA

CENTER ID:	BrabA.17534.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17534.a.A2.GE31692	Mature protein( BrabA.17534.a )		29	160

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.2431
RefSeq:	YP_418387.1
UniProt:	Q2YJ69

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRVKAIFQT ARLALAGGFL LAAMAGAGAA DRIAFVVPSA TVYPGQIVSD TALLEKQFFI NAPAATQYVL SLDQAVGKVA RKTLLPGKPI LVSALGEPSL VTRGIPAPLV FTAGTLTITA MGTPLESGVA GDFIKVRNVD SGIIVSGTVL ANGRIQVGMQ

NT Sequence

ATGGACCGTG TGAAGGCGAT TTTTCAAACG GCGCGGCTGG CTCTTGCTGG TGGCTTTCTG CTGGCAGCAA TGGCTGGCGC CGGTGCGGCA GACCGCATTG CTTTTGTCGT GCCTTCGGCG ACCGTCTATC CCGGACAAAT CGTGAGCGAT ACCGCGCTTC TGGAAAAGCA GTTCTTCATC AATGCGCCTG CGGCGACCCA ATATGTGCTG TCGCTGGATC AGGCGGTGGG CAAGGTGGCG CGCAAGACGC TGCTTCCCGG CAAGCCGATC CTCGTTTCGG CACTGGGCGA GCCGTCTCTG GTCACGCGCG GCATTCCTGC ACCACTGGTC TTTACCGCCG GCACGCTGAC TATAACTGCG ATGGGTACAC CGCTCGAATC CGGTGTGGCT GGCGATTTCA TCAAGGTTCG TAACGTCGAT AGCGGCATCA TCGTGTCCGG CACAGTGCTC GCAAATGGTC GTATTCAGGT GGGGATGCAG

Details for BrabA.17534.a.A2.GE31692

HARVESTED ON:	2/23/2011
SEQUENCED ON:	3/1/2011
EXPECTED MW:	16kDa
OBSERVED MW:	14kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAADRIAFV VPSATVYPGQ IVSDTALLEK QFFINAPAAT QYVLSLDQAV GKVARKTLLP GKPILVSALG EPSLVTRGIP APLVFTAGTL TITAMGTPLE SGVAGDFIKV RNVDSGIIVS GTVLANGRIQ VGMQ

Validated NT Sequence

ttattactgc atccccacct gaatacgacc atttgcgagc actgtgccgg acacgatgat gccgctatcg acgttacgaa ccttgatgaa atcgccagcc acaccggatt cgagcggtgt acccatcgca gttatagtca gcgtgccggc ggtaaagacc agtggtgcag gaatgccgcg cgtgaccaga gacggctcgc ccagtgccga aacgaggatc ggcttgccgg gaagcagcgt cttgcgcgcc accttgccca ccgcctgatc cagcgacagc acatattggg tcgccgcagg cgcattgatg aagaactgct tttccagaag cgcggtatcg ctcacgattt gtccgggata gacggtcgcc gaaggcacga caaaagcaat gcggtctgcc gccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAADRIAFV VPSATVYPGQ IVSDTALLEK QFFINAPAAT QYVLSLDQAV GKVARKTLLP GKPILVSALG EPSLVTRGIP APLVFTAGTL TITAMGTPLE SGVAGDFIKV RNVDSGIIVS GTVLANGRIQ VGMQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggcagac cgcattgctt ttgtcgtgcc ttcggcgacc gtctatcccg gacaaatcgt gagcgatacc gcgcttctgg aaaagcagtt cttcatcaat gcgcctgcgg cgacccaata tgtgctgtcg ctggatcagg cggtgggcaa ggtggcgcgc aagacgctgc ttcccggcaa gccgatcctc gtttcggcac tgggcgagcc gtctctggtc acgcgcggca ttcctgcacc actggtcttt accgccggca cgctgactat aactgcgatg ggtacaccgc tcgaatccgg tgtggctggc gatttcatca aggttcgtaa cgtcgatagc ggcatcatcg tgtccggcac agtgctcgca aatggtcgta ttcaggtggg gatgcagtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa