SSGCID: Target Page

BrabA.17520.a

HTH-type quorum sensing-dependent transcriptional regulator vjbR

CENTER ID:	BrabA.17520.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17520.a.A1.GE31688	Full length( BrabA.17520.a )		1	259

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17520.a.A1.PS01215	Full length( BrabA.17520.a )		1	259	unavailable

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.2395
RefSeq:	YP_418357.1
UniProt:	Q2YJ50

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLDLVHFPN YKKTFFGSSF QSDTLALLTR IRDEIGCRYV THTYRGRVGD CTKVNSADLT VLMTLPATWV ARYSSKNYFA IDPVFQEDAP YYRNDTSAIA RDLKEDADIC PAVAELLHDA EKHGLGNLFI AVSARNPKGV AGCTVFTFEV EDEDRTQFLA RMRPRLLSLA GIIHGTVCGC KDANSVASLL TPREVDCLRW AANGKTDGEI AEILSIARWT VVTYLQNAKI KLNCSNRTSA VATALSLGII DMPEVQHLV

NT Sequence

ATGAGTCTTG ATCTCGTTCA TTTTCCGAAT TACAAAAAAA CCTTTTTTGG TTCTTCCTTT CAAAGCGATA CTTTGGCGCT TCTAACCCGC ATCCGGGATG AAATAGGATG CCGCTACGTA ACGCATACCT ATCGTGGTCG CGTAGGCGAC TGCACGAAGG TGAATTCAGC CGATCTGACT GTTCTTATGA CGCTGCCTGC TACCTGGGTC GCGCGCTATT CCTCGAAAAA CTACTTTGCC ATTGACCCGG TTTTTCAGGA AGACGCTCCG TATTACCGCA ATGATACGAG CGCCATTGCC CGCGACCTGA AAGAAGATGC GGATATTTGC CCGGCCGTTG CCGAACTGTT GCATGATGCT GAAAAGCACG GCCTGGGAAA TCTTTTCATC GCCGTATCCG CGCGCAATCC CAAGGGCGTT GCGGGTTGTA CGGTTTTCAC TTTCGAGGTG GAGGACGAAG ACCGTACGCA ATTCCTTGCC AGGATGCGCC CGCGCCTTCT AAGCCTTGCC GGCATCATTC ATGGAACTGT CTGCGGCTGC AAGGATGCAA ATTCAGTTGC AAGCCTTCTC ACCCCGCGCG AGGTCGATTG CCTGCGCTGG GCTGCCAATG GCAAGACTGA TGGCGAAATC GCCGAAATCC TCAGCATCGC CCGCTGGACG GTGGTGACTT ATCTACAGAA TGCCAAAATC AAGCTGAACT GTTCCAATCG AACGTCGGCA GTGGCGACGG CGCTTTCCCT CGGCATTATC GATATGCCCG AGGTACAGCA TCTCGTC

Details for BrabA.17520.a.A1.GE31688

HARVESTED ON:	2/23/2011
SEQUENCED ON:	3/1/2011
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMSLDLVH FPNYKKTFFG SSFQSDTLAL LTRIRDEIGC RYVTHTYRGR VGDCTKVNSA DLTVLMTLPA TWVARYSSKN YFAIDPVFQE DAPYYRNDTS AIARDLKEDA DICPAVAELL HDAEKHGLGN LFIAVSARNP KGVAGCTVFT FEVEDEDRTQ FLARMRPRLL SLAGIIHGTV CGCKDANSVA SLLTPREVDC LRWAANGKTD GEIAEILSIA RWTVVTYLQN AKIKLNCSNR TSAVATALSL GIIDMPEVQH LV

Validated NT Sequence

ttattagacg agatgctgta cctcgggcat atcgataatg ccgagggaaa gcgccgtcgc cactgccgac gttcgattgg aacagttcag cttgattttg gcattctgta gataagtcac caccgtccag cgggcgatgc tgaggatttc ggcgatttcg ccatcagtct tgccattggc agcccagcgc aggcaatcga cctcgcgcgg ggtgagaagg cttgcaactg aatttgcatc cttgcagccg cagacagttc catgaatgat gccggcaagg cttagaaggc gcgggcgcat cctggcaagg aattgcgtac ggtcttcgtc ctccacctcg aaagtgaaaa ccgtacaacc cgcaacgccc ttgggattgc gcgcggatac ggcgatgaaa agatttccca ggccgtgctt ttcagcatca tgcaacagtt cggcaacggc cgggcaaata tccgcatctt ctttcaggtc gcgggcaatg gcgctcgtat cattgcggta atacggagcg tcttcctgaa aaaccgggtc aatggcaaag tagtttttcg aggaatagcg cgcgacccag gtagcaggca gcgtcataag aacagtcaga tcggctgaat tcaccttcgt gcagtcgcct acgcgaccac gataggtatg cgttacgtag cggcatccta tttcatcccg gatgcgggtt agaagcgcca aagtatcgct ttgaaaggaa gaaccaaaaa aggttttttt gtaattcgga aaatgaacga gatcaagact catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccnt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSLDLVHFP NYKKTFFGSS FQSDTLALLT RIRDEIGCRY VTHTYRGRVG DCTKVNSADL TVLMTLPATW VARYSSKNYF AIDPVFQEDA PYYRNDTSAI ARDLKEDADI CPAVAELLHD AEKHGLGNLF IAVSARNPKG VAGCTVFTFE VEDEDRTQFL ARMRPRLLSL AGIIHGTVCG CKDANSVASL LTPREVDCLR WAANGKTDGE IAEILSIARW TVVTYLQNAK IKLNCSNRTS AVATALSLGI IDMPEVQHLV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtcttgat ctcgttcatt ttccgaatta caaaaaaacc ttttttggtt cttcctttca aagcgatact ttggcgcttc taacccgcat ccgggatgaa ataggatgcc gctacgtaac gcatacctat cgtggtcgcg taggcgactg cacgaaggtg aattcagccg atctgactgt tcttatgacg ctgcctgcta cctgggtcgc gcgctattcc tcgaaaaact actttgccat tgacccggtt tttcaggaag acgctccgta ttaccgcaat gatacgagcg ccattgcccg cgacctgaaa gaagatgcgg atatttgccc ggccgttgcc gaactgttgc atgatgctga aaagcacggc ctgggaaatc ttttcatcgc cgtatccgcg cgcaatccca agggcgttgc gggttgtacg gttttcactt tcgaggtgga ggacgaagac cgtacgcaat tccttgccag gatgcgcccg cgccttctaa gccttgccgg catcattcat ggaactgtct gcggctgcaa ggatgcaaat tcagttgcaa gccttctcac cccgcgcgag gtcgattgcc tgcgctgggc tgccaatggc aagactgatg gcgaaatcgc cgaaatcctc agcatcgccc gctggacggt ggtgacttat ctacagaatg ccaaaatcaa gctgaactgt tccaatcgaa cgtcggcagt ggcgacggcg ctttccctcg gcattatcga tatgcccgag gtacagcatc tcgtctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for BrabA.17520.a.A1.PS01215

PURIFICATION DATe:	9/21/2011
CONCENTRATION:	8.45mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	data unavailable
VIAL VOLUME:	data unavailable
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTCTTGAT CTCGTTCATT TTCCGAATTA CAAAAAAACC TTTTTTGGTT CTTCCTTTCA AAGCGATACT TTGGCGCTTC TAACCCGCAT CCGGGATGAA ATAGGATGCC GCTACGTAAC GCATACCTAT CGTGGTCGCG TAGGCGACTG CACGAAGGTG AATTCAGCCG ATCTGACTGT TCTTATGACG CTGCCTGCTA CCTGGGTCGC GCGCTATTCC TCGAAAAACT ACTTTGCCAT TGACCCGGTT TTTCAGGAAG ACGCTCCGTA TTACCGCAAT GATACGAGCG CCATTGCCCG CGACCTGAAA GAAGATGCGG ATATTTGCCC GGCCGTTGCC GAACTGTTGC ATGATGCTGA AAAGCACGGC CTGGGAAATC TTTTCATCGC CGTATCCGCG CGCAATCCCA AGGGCGTTGC GGGTTGTACG GTTTTCACTT TCGAGGTGGA GGACGAAGAC CGTACGCAAT TCCTTGCCAG GATGCGCCCG CGCCTTCTAA GCCTTGCCGG CATCATTCAT GGAACTGTCT GCGGCTGCAA GGATGCAAAT TCAGTTGCAA GCCTTCTCAC CCCGCGCGAG GTCGATTGCC TGCGCTGGGC TGCCAATGGC AAGACTGATG GCGAAATCGC CGAAATCCTC AGCATCGCCC GCTGGACGGT GGTGACTTAT CTACAGAATG CCAAAATCAA GCTGAACTGT TCCAATCGAA CGTCGGCAGT GGCGACGGCG CTTTCCCTCG GCATTATCGA TATGCCCGAG GTACAGCATC TCGTCTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA