BrabA.17502.a
Ribosomal-protein-alanine N-acetyltransferase
| CENTER ID: | BrabA.17502.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.17502.a.A1.GE32520 | Full length( BrabA.17502.a ) | 1 | 152 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
7N1L
DEPOSITED: 5/27/2021
DETERMINATION: XRay
CLONE: BrabA.17502.a.A1.GE32520
PROTEIN: BrabA.17502.a.A1.PS01129
Molecular models
| METHOD | RESULTS |
|---|---|
| comparative modelling | Robetta_81918 |
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|359391.4.peg.1983 |
| RefSeq: | YP_415246.1 |
| UniProt: | Q2YLP8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTKDHAVDIA LLHLRDAHEF APLLASYAQA LKRGAPRRPD DFYAEHLLQD RAAEALGARV DGNLVGFVIF YDLPEPVTGL RAGQVDHIYV HHDHRGKGIA KALIDVLADK AEERSWSKLV LNAPRVPEDG RKLYEQIAAA ADWSSYVIRF GN
NT Sequence
ATGACCAAAG ATCATGCCGT CGATATTGCA CTTCTTCATT TGCGCGACGC CCATGAATTT GCGCCGCTGC TTGCAAGCTA TGCGCAGGCA CTGAAACGCG GTGCGCCACG CCGGCCGGAT GATTTTTATG CCGAGCATCT GCTTCAGGAT CGCGCAGCAG AAGCACTTGG TGCCCGTGTC GACGGCAATC TGGTCGGCTT TGTCATCTTC TATGATCTGC CTGAACCCGT CACTGGCCTG CGCGCCGGGC AGGTAGATCA CATCTATGTC CATCATGACC ATCGCGGCAA AGGCATCGCC AAGGCGCTTA TCGATGTGCT GGCCGACAAA GCCGAAGAGC GTAGCTGGTC GAAGCTTGTC CTCAATGCGC CGCGTGTACC CGAAGACGGC CGCAAGCTCT ATGAACAGAT CGCGGCGGCT GCGGACTGGT CGAGCTATGT GATTCGATTC GGCAAT
Details for BrabA.17502.a.A1.GE32520
| HARVESTED ON: | 5/17/2011 |
| SEQUENCED ON: | 2/2/2017 |
| EXPECTED MW: | 19kDa |
| OBSERVED MW: | 19kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTKDHAVDI ALLHLRDAHE FAPLLASYAQ ALKRGAPRRP DDFYAEHLLQ DRAAEALGAR VDGNLVGFVI FYDLPEPVTG LRAGQVDHIY VHHDHRGKGI AKALIDVLAD KAEERSWSKL VLNAPRVPED GRKLYEQIAA AADWSSYVIR FGN
Validated NT Sequence
ttattaattg ccgaatcgaa tcacatagct cgaccagtcc gcagccgccg cgatctgttc atagagcttg cggccgtctt cgggtacacg cggcgcattg aggacaagct tcgaccagct acgctcttcg gctttgtcgg ccagcacatc gataagcgcc ttggcgatgc ctttgccgcg atggtcatga tggacataga tgtgatctac ctgcccggcg cgcaggccag tgacgggttc aggcagatca tagaagatga caaagccgac cagattgccg tcgacacggg caccaagtgc ttctgctgcg cgatcctgaa gcagatgctc ggcataaaaa tcatccggcc ggcgtggcgc accgcgtttc agtgcctgcg catagcttgc aagcagcggc gcaaattcat gggcgtcgcg caaatgaaga agtgcaatat cgacggcatg atctttggtc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTKDHAVDI ALLHLRDAHE FAPLLASYAQ ALKRGAPRRP DDFYAEHLLQ DRAAEALGAR VDGNLVGFVI FYDLPEPVTG LRAGQVDHIY VHHDHRGKGI AKALIDVLAD KAEERSWSKL VLNAPRVPED GRKLYEQIAA AADWSSYVIR FGN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccaaagat catgccgtcg atattgcact tcttcatttg cgcgacgccc atgaatttgc gccgctgctt gcaagctatg cgcaggcact gaaacgcggt gcgccacgcc ggccggatga tttttatgcc gagcatctgc ttcaggatcg cgcagcagaa gcacttggtg cccgtgtcga cggcaatctg gtcggctttg tcatcttcta tgatctgcct gaacccgtca ctggcctgcg cgccgggcag gtagatcaca tctatgtcca tcatgaccat cgcggcaaag gcatcgccaa ggcgcttatc gatgtgctgg ccgacaaagc cgaagagcgt agctggtcga agcttgtcct caatgcgccg cgtgtacccg aagacggccg caagctctat gaacagatcg cggcggctgc ggactggtcg agctatgtga ttcgattcgg caattaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa