BrabA.17468.b
Acetyltransferase, GNAT family
| CENTER ID: | BrabA.17468.b |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.17468.b.B1.GE37962 | Full length( BrabA.17468.b ) | 1 | 164 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.17468.b.B1.PS02320 | Full length( BrabA.17468.b ) | 1 | 164 |
Structures
4ZBG
DEPOSITED: 4/14/2015
DETERMINATION: XRay
CLONE: BrabA.17468.b.B1.GE37962
PROTEIN: BrabA.17468.b.B1.PS02320
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|359391.4.peg.1626 |
| RefSeq: | YP_414933.1 |
| UniProt: | Q2YS09 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHIRKERPQD AAEIRQVTEA AFRPVAYSNQ KEGEIVDALR AAKALTLSLV AEEDGQVLGH IAFSPVLIGG AEKGWYGLGP VSVLPARQGE GIGGKLIREG LAQLRGAGAR GCVLLGDLGY YRRFGFKADA RQKLPGVPPE YFQCLAFGPD MPQGDVAYHA AFDA
NT Sequence
ATGCATATCC GCAAGGAAAG GCCACAAGAC GCCGCCGAAA TCCGTCAGGT GACAGAGGCG GCTTTTCGCC CTGTTGCTTA TAGCAACCAG AAGGAAGGGG AGATTGTCGA TGCGCTTCGC GCGGCAAAGG CGCTTACCCT GTCGCTGGTT GCGGAGGAAG ACGGGCAGGT TCTGGGCCAT ATCGCCTTTT CGCCGGTCCT GATCGGCGGT GCGGAAAAGG GTTGGTACGG GCTTGGTCCC GTTTCAGTGC TGCCCGCCCG GCAAGGTGAG GGGATTGGCG GCAAGCTGAT CCGCGAGGGC CTTGCGCAAT TGCGCGGTGC CGGGGCAAGG GGCTGTGTTC TTCTTGGCGA CCTCGGCTAT TACAGACGCT TTGGTTTCAA GGCCGATGCG CGGCAGAAAC TGCCGGGGGT GCCGCCCGAA TATTTCCAGT GTCTGGCCTT CGGTCCGGAT ATGCCGCAAG GCGATGTTGC CTATCATGCG GCATTCGATG CA
Details for BrabA.17468.b.B1.GE37962
| HARVESTED ON: | 5/12/2014 |
| SEQUENCED ON: | 5/4/2014 |
| EXPECTED MW: | 19kDa |
| OBSERVED MW: | 17kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMH IRKERPQDAA EIRQVTEAAF RPVAYSNQKE GEIVDALRAA KALTLSLVAE EDGQVLGHIA FSPVLIGGAE KGWYGLGPVS VLPARQGEGI GGKLIREGLA QLRGAGARGC VLLGDLGYYR RFGFKADARQ KLPGVPPEYF QCLAFGPDMP QGDVAYHAAF DA
Validated NT Sequence
atattttntt taactttaag aaggagatat accatggctc accaccacca ccaccatatg catatccgca aggaaaggcc acaagacgcc gccgaaatcc gtcaggtgac agaggcggct tttcgccctg ttgcttatag caaccagaag gaaggggaga ttgtcgatgc gcttcgcgcg gcaaaggcgc ttaccctgtc gctggttgcg gaggaagacg ggcaggttct gggccatatc gccttttcgc cggtcctgat cggcggtgcg gaaaagggtt ggtacgggct tggtcccgtt tcagtgctgc ccgcccggca aggtgagggg attggcggca agctgatccg cgagggcctt gcgcaattgc gcggtgccgg ggcaaggggc tgtgttcttc ttggcgacct cggctattac agacgctttg gtttcaaggc cgatgcgcgg cagaaactgc cgggggtgcc gcccgaatat ttccagtgtc tggccttcgg tccggatatg ccgcaaggcg atgttgccta tcatgcggca ttcgatgcat aagtgagtaa gataggatcc ggctgctann ncccccccaa gaagaaannt aattanngat nnacnggtat caaccatgtt gctcaaaaag cgattctcac tattcttctt ttgntagcaa gacagggaca gtgagcgctc gcgcccacga ggaaaacccc aaacgccatc ataatcacgg accatcgggc ctgcngaaat cctcnatatn cggtgtcgga agaccacacn ntntcgcagg gcctccatgc anagcacgct gngtattccg tctttgaggt catcaagacg acgacnntga agganatnag agacatgcga cacatgagtn cnnnnncnaa ctgcntnacc tcngccngta tccattcnna cgcnnncggn nattagccaa acgtttanna tgnctgcnag ncnncnncnn actacnntan tacatcantt cnntgncnng ctctcagcca aggacgaaaa aaacagcccg ganaaantat ttaaannng
Expected Protein Sequence
MAHHHHHHMH IRKERPQDAA EIRQVTEAAF RPVAYSNQKE GEIVDALRAA KALTLSLVAE EDGQVLGHIA FSPVLIGGAE KGWYGLGPVS VLPARQGEGI GGKLIREGLA QLRGAGARGC VLLGDLGYYR RFGFKADARQ KLPGVPPEYF QCLAFGPDMP QGDVAYHAAF DA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcata tccgcaagga aaggccacaa gacgccgccg aaatccgtca ggtgacagag gcggcttttc gccctgttgc ttatagcaac cagaaggaag gggagattgt cgatgcgctt cgcgcggcaa aggcgcttac cctgtcgctg gttgcggagg aagacgggca ggttctgggc catatcgcct tttcgccggt cctgatcggc ggtgcggaaa agggttggta cgggcttggt cccgtttcag tgctgcccgc ccggcaaggt gaggggattg gcggcaagct gatccgcgag ggccttgcgc aattgcgcgg tgccggggca aggggctgtg ttcttcttgg cgacctcggc tattacagac gctttggttt caaggccgat gcgcggcaga aactgccggg ggtgccgccc gaatatttcc agtgtctggc cttcggtccg gatatgccgc aaggcgatgt tgcctatcat gcggcattcg atgcatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BrabA.17468.b.B1.PS02320
| PURIFICATION DATe: | 2/5/2015 |
| CONCENTRATION: | 10.36mg/ml |
| OBSERVED MW: | 18kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMH IRKERPQDAA EIRQVTEAAF RPVAYSNQKE GEIVDALRAA KALTLSLVAE EDGQVLGHIA FSPVLIGGAE KGWYGLGPVS VLPARQGEGI GGKLIREGLA QLRGAGARGC VLLGDLGYYR RFGFKADARQ KLPGVPPEYF QCLAFGPDMP QGDVAYHAAF DA
Validated NT Sequence
atattttntt taactttaag aaggagatat accatggctc accaccacca ccaccatatg catatccgca aggaaaggcc acaagacgcc gccgaaatcc gtcaggtgac agaggcggct tttcgccctg ttgcttatag caaccagaag gaaggggaga ttgtcgatgc gcttcgcgcg gcaaaggcgc ttaccctgtc gctggttgcg gaggaagacg ggcaggttct gggccatatc gccttttcgc cggtcctgat cggcggtgcg gaaaagggtt ggtacgggct tggtcccgtt tcagtgctgc ccgcccggca aggtgagggg attggcggca agctgatccg cgagggcctt gcgcaattgc gcggtgccgg ggcaaggggc tgtgttcttc ttggcgacct cggctattac agacgctttg gtttcaaggc cgatgcgcgg cagaaactgc cgggggtgcc gcccgaatat ttccagtgtc tggccttcgg tccggatatg ccgcaaggcg atgttgccta tcatgcggca ttcgatgcat aagtgagtaa gataggatcc ggctgctann ncccccccaa gaagaaannt aattanngat nnacnggtat caaccatgtt gctcaaaaag cgattctcac tattcttctt ttgntagcaa gacagggaca gtgagcgctc gcgcccacga ggaaaacccc aaacgccatc ataatcacgg accatcgggc ctgcngaaat cctcnatatn cggtgtcgga agaccacacn ntntcgcagg gcctccatgc anagcacgct gngtattccg tctttgaggt catcaagacg acgacnntga agganatnag agacatgcga cacatgagtn cnnnnncnaa ctgcntnacc tcngccngta tccattcnna cgcnnncggn nattagccaa acgtttanna tgnctgcnag ncnncnncnn actacnntan tacatcantt cnntgncnng ctctcagcca aggacgaaaa aaacagcccg ganaaantat ttaaannng
Expressed Protein Sequence
MAHHHHHHMH IRKERPQDAA EIRQVTEAAF RPVAYSNQKE GEIVDALRAA KALTLSLVAE EDGQVLGHIA FSPVLIGGAE KGWYGLGPVS VLPARQGEGI GGKLIREGLA QLRGAGARGC VLLGDLGYYR RFGFKADARQ KLPGVPPEYF QCLAFGPDMP QGDVAYHAAF DA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCATA TCCGCAAGGA AAGGCCACAA GACGCCGCCG AAATCCGTCA GGTGACAGAG GCGGCTTTTC GCCCTGTTGC TTATAGCAAC CAGAAGGAAG GGGAGATTGT CGATGCGCTT CGCGCGGCAA AGGCGCTTAC CCTGTCGCTG GTTGCGGAGG AAGACGGGCA GGTTCTGGGC CATATCGCCT TTTCGCCGGT CCTGATCGGC GGTGCGGAAA AGGGTTGGTA CGGGCTTGGT CCCGTTTCAG TGCTGCCCGC CCGGCAAGGT GAGGGGATTG GCGGCAAGCT GATCCGCGAG GGCCTTGCGC AATTGCGCGG TGCCGGGGCA AGGGGCTGTG TTCTTCTTGG CGACCTCGGC TATTACAGAC GCTTTGGTTT CAAGGCCGAT GCGCGGCAGA AACTGCCGGG GGTGCCGCCC GAATATTTCC AGTGTCTGGC CTTCGGTCCG GATATGCCGC AAGGCGATGT TGCCTATCAT GCGGCATTCG ATGCATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT