BrabA.17437.a

Putative uncharacterized protein

CENTER ID: BrabA.17437.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17437.a.A1.GE31641 Full length( BrabA.17437.a ) 1 338
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17437.a.A1.PS01090 Full length( BrabA.17437.a ) 1 338

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.273
RefSeq: YP_413734.1
UniProt: Q2YPB3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNIHASNLDI EKFRKVYALV TGGATDGERV AAQARARKIA ERAGMSLNDA VSQLDSQPKP KPANFFEGFA DWMEEKEPGY KAKRAREVVE REQRYASRRA EILKQFGTAK AFLDPTPNER LILKAAEPFI AELGEPYEDA CGTWRRPISS FAGVHSHFFN LDDVDPEAIM AIKAAIPFPE TIRGAFEELK VWDKLNDDRA HFYGHHEYYY ELPVELRIEL LREVMRTQPV TSWGDLEARF HYKSYAWQRQ WIDPKDFDDP EWSRLFDDVR ILRALAEKPF REPVQNGRRT NAEKRSAVLS MLDTNPELSD REICRRVGVS PQTVGNWRRR RNDRLSQP
NT Sequence
ATGAACATTC ATGCGTCCAA TTTGGACATA GAGAAATTCC GCAAGGTTTA TGCGCTGGTC ACAGGCGGCG CGACTGACGG GGAGAGAGTA GCAGCCCAAG CGAGAGCCAG AAAGATTGCA GAAAGAGCGG GCATGTCGCT GAATGACGCT GTGTCCCAAT TGGACAGTCA GCCAAAGCCG AAGCCGGCCA ATTTCTTCGA GGGCTTCGCG GACTGGATGG AAGAAAAGGA GCCTGGCTAC AAGGCGAAGC GGGCGCGGGA GGTTGTTGAG CGTGAACAGC GCTACGCGTC AAGGCGTGCT GAAATCCTGA AACAGTTTGG GACCGCTAAG GCCTTTCTTG ACCCTACACC GAACGAACGG CTGATCCTCA AGGCAGCGGA ACCCTTCATT GCTGAGTTGG GCGAGCCATA CGAGGACGCT TGCGGGACAT GGCGTCGGCC AATTTCGTCA TTCGCCGGTG TTCACAGCCA CTTTTTCAAT CTGGATGACG TTGACCCGGA AGCTATCATG GCTATCAAGG CGGCAATTCC ATTCCCCGAA ACCATACGTG GAGCTTTCGA GGAATTGAAG GTGTGGGACA AACTGAACGA CGATAGGGCT CATTTTTACG GTCACCACGA ATATTATTAC GAGCTTCCCG TTGAGCTTCG AATTGAACTT CTACGAGAAG TGATGCGAAC CCAGCCGGTT ACTTCATGGG GCGATCTGGA AGCCCGTTTT CATTACAAGT CGTATGCGTG GCAGCGGCAA TGGATTGATC CGAAGGATTT TGATGACCCG GAATGGTCGC GGCTGTTTGA TGATGTTCGC ATTCTTCGCG CTTTGGCCGA AAAGCCTTTC CGTGAACCCG TCCAAAATGG ACGCCGCACC AATGCGGAAA AGCGTTCTGC CGTCCTGTCG ATGTTGGACA CTAACCCCGA ATTGTCAGAC CGTGAGATTT GCCGGCGTGT CGGCGTTAGT CCTCAGACCG TAGGCAATTG GCGCAGACGC AGGAATGACA GATTGTCGCA GCCT
Details for BrabA.17437.a.A1.GE31641
HARVESTED ON: 3/1/2011
SEQUENCED ON: 3/7/2011
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MNIHASNLDI EKFRKVYALV TGGATDGERV AAQARARKIA ERAGMSLNDA VSQLDSQPKP KPANFFEGFA DWMEEKEPGY KAKRAREVVE REQRYASRRA EILKQFGTAK AFLDPTPNER LILKAAEPFI AELGEPYEDA CGTWRRPISS FAGVHSHFFN LDDVDPEAIM AIKAAIPFPE TIRGAFEELK VWDKLNDDRA HFYGHHEYYY ELPVELRIEL LREVMRTQPV TSWGDLEARF HYKSYAWQRQ WIDPKDFDDP EWSRLFDDVR ILRALAEKPF REPVQNGRRT NAEKRSAVLS MLDTNPELSD REICRRVGVS PQTVGNWRRR RNDRL
Validated NT Sequence
cgacaatctg tcattcctgc gtctgcgcca attgcctacg gtctgaggac taacgccgac acgccggcaa atctcacggt ctgacaattc ggggttagtg tccaacatcg acaggacggc agaacgcttt tccgcattgg tgcggcgtcc attttggacg ggttcacgga aaggcttttc ggccaaagcg cgaagaatgc gaacatcatc aaacagccgc gaccattccg ggtcatcaaa atccttcgga tcaatccatt gccgctgcca cgcatacgac ttgtaatgaa aacgggcttc cagatcgccc catgaagtaa ccggctgggt tcgcatcact tctcgtagaa gttcaattcg aagctcaacg ggaagctcgt aataatattc gtggtgaccg taaaaatgag ccctatcgtc gttcagtttg tcccacacct tcaattcctc gaaagctcca cgtatggttt cggggaatgg aattgccgcc ttgatagcca tgatagcttc cgggtcaacg tcatccagat tgaaaaagtg gctgtgaaca ccggcgaatg acgaaattgg ccgacgccat gtcccgcaag cgtcctcgta tggctcgccc aactcagcaa tgaagggttc cgctgccttg aggatcagcc gttcgttcgg tgtagggtca agaaaggcct tagcggtccc aaactgtttc aggatttcag cacgccttga cgcgtagcgc tgttcacgct caacaacctc ccgcgcccgc ttcgccttgt agccaggctc cttttcttcc atccagtccg cgaagccctc gaagaaattg gccggcttcg gctttggctg actgtccaat tgggacacag cgtcattcag cgacatgccc gctctttctg caatctttct ggctctcgct tgggctgcta ctctctcccc gtcagtcgcg ccgcctgtga ccagcgcata aaccttgcgg aatttctcta tgtccaaatt ggacgcatga atgttcatcg aaccaggacc ctgggtctga gcttccaggg taccc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNIHASNLD IEKFRKVYAL VTGGATDGER VAAQARARKI AERAGMSLND AVSQLDSQPK PKPANFFEGF ADWMEEKEPG YKAKRAREVV EREQRYASRR AEILKQFGTA KAFLDPTPNE RLILKAAEPF IAELGEPYED ACGTWRRPIS SFAGVHSHFF NLDDVDPEAI MAIKAAIPFP ETIRGAFEEL KVWDKLNDDR AHFYGHHEYY YELPVELRIE LLREVMRTQP VTSWGDLEAR FHYKSYAWQR QWIDPKDFDD PEWSRLFDDV RILRALAEKP FREPVQNGRR TNAEKRSAVL SMLDTNPELS DREICRRVGV SPQTVGNWRR RRNDRLSQP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacattcat gcgtccaatt tggacataga gaaattccgc aaggtttatg cgctggtcac aggcggcgcg actgacgggg agagagtagc agcccaagcg agagccagaa agattgcaga aagagcgggc atgtcgctga atgacgctgt gtcccaattg gacagtcagc caaagccgaa gccggccaat ttcttcgagg gcttcgcgga ctggatggaa gaaaaggagc ctggctacaa ggcgaagcgg gcgcgggagg ttgttgagcg tgaacagcgc tacgcgtcaa ggcgtgctga aatcctgaaa cagtttggga ccgctaaggc ctttcttgac cctacaccga acgaacggct gatcctcaag gcagcggaac ccttcattgc tgagttgggc gagccatacg aggacgcttg cgggacatgg cgtcggccaa tttcgtcatt cgccggtgtt cacagccact ttttcaatct ggatgacgtt gacccggaag ctatcatggc tatcaaggcg gcaattccat tccccgaaac catacgtgga gctttcgagg aattgaaggt gtgggacaaa ctgaacgacg atagggctca tttttacggt caccacgaat attattacga gcttcccgtt gagcttcgaa ttgaacttct acgagaagtg atgcgaaccc agccggttac ttcatggggc gatctggaag cccgttttca ttacaagtcg tatgcgtggc agcggcaatg gattgatccg aaggattttg atgacccgga atggtcgcgg ctgtttgatg atgttcgcat tcttcgcgct ttggccgaaa agcctttccg tgaacccgtc caaaatggac gccgcaccaa tgcggaaaag cgttctgccg tcctgtcgat gttggacact aaccccgaat tgtcagaccg tgagatttgc cggcgtgtcg gcgttagtcc tcagaccgta ggcaattggc gcagacgcag gaatgacaga ttgtcgcagc cttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BrabA.17437.a.A1.PS01090
PURIFICATION DATe: 5/18/2011
CONCENTRATION: 25.45mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MNIHASNLDI EKFRKVYALV TGGATDGERV AAQARARKIA ERAGMSLNDA VSQLDSQPKP KPANFFEGFA DWMEEKEPGY KAKRAREVVE REQRYASRRA EILKQFGTAK AFLDPTPNER LILKAAEPFI AELGEPYEDA CGTWRRPISS FAGVHSHFFN LDDVDPEAIM AIKAAIPFPE TIRGAFEELK VWDKLNDDRA HFYGHHEYYY ELPVELRIEL LREVMRTQPV TSWGDLEARF HYKSYAWQRQ WIDPKDFDDP EWSRLFDDVR ILRALAEKPF REPVQNGRRT NAEKRSAVLS MLDTNPELSD REICRRVGVS PQTVGNWRRR RNDRL
Validated NT Sequence
cgacaatctg tcattcctgc gtctgcgcca attgcctacg gtctgaggac taacgccgac acgccggcaa atctcacggt ctgacaattc ggggttagtg tccaacatcg acaggacggc agaacgcttt tccgcattgg tgcggcgtcc attttggacg ggttcacgga aaggcttttc ggccaaagcg cgaagaatgc gaacatcatc aaacagccgc gaccattccg ggtcatcaaa atccttcgga tcaatccatt gccgctgcca cgcatacgac ttgtaatgaa aacgggcttc cagatcgccc catgaagtaa ccggctgggt tcgcatcact tctcgtagaa gttcaattcg aagctcaacg ggaagctcgt aataatattc gtggtgaccg taaaaatgag ccctatcgtc gttcagtttg tcccacacct tcaattcctc gaaagctcca cgtatggttt cggggaatgg aattgccgcc ttgatagcca tgatagcttc cgggtcaacg tcatccagat tgaaaaagtg gctgtgaaca ccggcgaatg acgaaattgg ccgacgccat gtcccgcaag cgtcctcgta tggctcgccc aactcagcaa tgaagggttc cgctgccttg aggatcagcc gttcgttcgg tgtagggtca agaaaggcct tagcggtccc aaactgtttc aggatttcag cacgccttga cgcgtagcgc tgttcacgct caacaacctc ccgcgcccgc ttcgccttgt agccaggctc cttttcttcc atccagtccg cgaagccctc gaagaaattg gccggcttcg gctttggctg actgtccaat tgggacacag cgtcattcag cgacatgccc gctctttctg caatctttct ggctctcgct tgggctgcta ctctctcccc gtcagtcgcg ccgcctgtga ccagcgcata aaccttgcgg aatttctcta tgtccaaatt ggacgcatga atgttcatcg aaccaggacc ctgggtctga gcttccaggg taccc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNIHASNLD IEKFRKVYAL VTGGATDGER VAAQARARKI AERAGMSLND AVSQLDSQPK PKPANFFEGF ADWMEEKEPG YKAKRAREVV EREQRYASRR AEILKQFGTA KAFLDPTPNE RLILKAAEPF IAELGEPYED ACGTWRRPIS SFAGVHSHFF NLDDVDPEAI MAIKAAIPFP ETIRGAFEEL KVWDKLNDDR AHFYGHHEYY YELPVELRIE LLREVMRTQP VTSWGDLEAR FHYKSYAWQR QWIDPKDFDD PEWSRLFDDV RILRALAEKP FREPVQNGRR TNAEKRSAVL SMLDTNPELS DREICRRVGV SPQTVGNWRR RRNDRLSQP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAACATTCAT GCGTCCAATT TGGACATAGA GAAATTCCGC AAGGTTTATG CGCTGGTCAC AGGCGGCGCG ACTGACGGGG AGAGAGTAGC AGCCCAAGCG AGAGCCAGAA AGATTGCAGA AAGAGCGGGC ATGTCGCTGA ATGACGCTGT GTCCCAATTG GACAGTCAGC CAAAGCCGAA GCCGGCCAAT TTCTTCGAGG GCTTCGCGGA CTGGATGGAA GAAAAGGAGC CTGGCTACAA GGCGAAGCGG GCGCGGGAGG TTGTTGAGCG TGAACAGCGC TACGCGTCAA GGCGTGCTGA AATCCTGAAA CAGTTTGGGA CCGCTAAGGC CTTTCTTGAC CCTACACCGA ACGAACGGCT GATCCTCAAG GCAGCGGAAC CCTTCATTGC TGAGTTGGGC GAGCCATACG AGGACGCTTG CGGGACATGG CGTCGGCCAA TTTCGTCATT CGCCGGTGTT CACAGCCACT TTTTCAATCT GGATGACGTT GACCCGGAAG CTATCATGGC TATCAAGGCG GCAATTCCAT TCCCCGAAAC CATACGTGGA GCTTTCGAGG AATTGAAGGT GTGGGACAAA CTGAACGACG ATAGGGCTCA TTTTTACGGT CACCACGAAT ATTATTACGA GCTTCCCGTT GAGCTTCGAA TTGAACTTCT ACGAGAAGTG ATGCGAACCC AGCCGGTTAC TTCATGGGGC GATCTGGAAG CCCGTTTTCA TTACAAGTCG TATGCGTGGC AGCGGCAATG GATTGATCCG AAGGATTTTG ATGACCCGGA ATGGTCGCGG CTGTTTGATG ATGTTCGCAT TCTTCGCGCT TTGGCCGAAA AGCCTTTCCG TGAACCCGTC CAAAATGGAC GCCGCACCAA TGCGGAAAAG CGTTCTGCCG TCCTGTCGAT GTTGGACACT AACCCCGAAT TGTCAGACCG TGAGATTTGC CGGCGTGTCG GCGTTAGTCC TCAGACCGTA GGCAATTGGC GCAGACGCAG GAATGACAGA TTGTCGCAGC CTTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA