SSGCID: Target Page

BrabA.17411.a

Condensation domain

CENTER ID:	BrabA.17411.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17411.a.A1.GE31640	Full length( BrabA.17411.a )		1	469

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17411.a.A1.PW31542	Full length( BrabA.17411.a )		1	469

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.2293
RefSeq:	YP_418263.1
UniProt:	Q2YL93

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVPICQPGDW PTLNEACRAE RNEMARIDFA DGWMPLTLPQ LDFWEEFSFH PDQPVSTVAH CVEIEGDVDV EKLLRAISQT VREAEVLSIR FSDEKEQGRP VQLCDPAHMP DVDFVDLSAF PDPRAEAGRR METDLSAPLD LRRDRMSAHA LYRIGARQYI WYIRAHHIII DGYGLALIEQ RCGQLYSHFC GQGESGHPFH PFASFIHEED AYRASPRWEA DRKFWQDYLD GPVRLPILDR GAEDYGEAGY HHDVAFRDDI SIGLRAMAKA TDIGWPDLLV LLSGLYLSRM LPMQGEDEGL TLWLPFMSRW GSVGAHMPGL LVNILPFHLE FDSGETLFSA LRRNMDMLKK HRLHGRYRIE QISADRGLVS GSRFFFSPLI NVLPFSTPQF AGCRVAREVL ANGPGDGFNL TYRADVDGAG LTLKIEAASN MMDAQTFARI AAGLPAFLKA SLAPHLIERP VSDLRRRLP

NT Sequence

TTGGTCCCAA TTTGTCAACC GGGTGATTGG CCGACGCTGA ACGAAGCTTG CCGAGCCGAG AGGAATGAGA TGGCCAGGAT AGATTTTGCA GATGGCTGGA TGCCGCTGAC ATTGCCCCAG CTCGATTTCT GGGAAGAGTT TTCCTTCCAC CCCGACCAGC CGGTCTCCAC GGTCGCGCAT TGCGTGGAAA TCGAAGGCGA TGTGGATGTG GAAAAATTGC TGCGCGCCAT TTCGCAGACC GTCCGTGAGG CGGAGGTTCT TTCCATCCGC TTTTCCGATG AAAAGGAGCA GGGGCGTCCG GTGCAATTAT GCGACCCGGC GCATATGCCG GACGTGGATT TTGTTGATCT CAGCGCCTTT CCCGATCCGC GGGCAGAGGC TGGGCGGCGT ATGGAAACCG ATCTGTCCGC ACCGCTTGAT CTGCGCCGGG ACAGGATGTC CGCCCATGCG CTTTATCGCA TCGGCGCGCG CCAATATATC TGGTATATCC GCGCCCATCA CATCATTATC GACGGTTACG GGCTGGCGCT GATCGAACAG CGTTGCGGCC AGCTTTACAG CCATTTTTGC GGCCAGGGCG AAAGTGGCCA CCCCTTCCAT CCTTTTGCAA GCTTCATTCA TGAGGAGGAC GCCTATCGGG CAAGCCCCCG CTGGGAGGCC GACCGCAAAT TCTGGCAGGA TTATCTGGAC GGGCCTGTGC GCCTTCCCAT CCTCGATCGG GGCGCGGAAG ATTATGGCGA GGCGGGCTAT CATCATGACG TCGCCTTTCG CGACGATATT TCCATAGGCC TTCGCGCGAT GGCAAAAGCC ACGGATATCG GCTGGCCCGA TCTTCTGGTG CTTTTATCGG GGCTCTATCT CTCGCGGATG CTTCCGATGC AGGGAGAGGA CGAGGGGCTG ACGCTGTGGC TGCCTTTCAT GAGCCGCTGG GGCAGCGTGG GCGCACATAT GCCGGGGCTT CTGGTCAATA TCCTGCCGTT CCATCTTGAA TTCGATTCCG GCGAAACATT GTTCTCTGCG TTACGGCGCA ATATGGACAT GCTGAAAAAG CACCGGCTGC ACGGGCGCTA TCGCATCGAA CAGATTTCCG CCGACCGCGG CCTCGTCAGT GGAAGCCGCT TTTTCTTCTC GCCGCTGATT AATGTTCTGC CATTCAGCAC GCCGCAATTT GCAGGTTGCC GGGTTGCGCG TGAGGTTCTG GCCAATGGCC CCGGCGACGG CTTCAACCTG ACCTATCGTG CCGATGTCGA TGGCGCTGGC CTTACCTTGA AAATCGAAGC GGCATCGAAC ATGATGGATG CGCAAACCTT TGCCCGCATT GCGGCGGGCC TTCCGGCCTT TCTCAAGGCA AGCCTTGCGC CCCATCTCAT CGAGCGTCCG GTGAGCGATC TTCGCCGCCG TTTGCCA

Details for BrabA.17411.a.A1.GE31640

HARVESTED ON:	3/1/2011
SEQUENCED ON:	3/7/2011
EXPECTED MW:	55kDa
OBSERVED MW:	55kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	15
PERCENT COVERAGE:	38

Validated AA Sequence

MXXXAXXXXX XXXXXXXXXR XXXXXPXSXX GSXXGXXXNK XXAXXXXXXX APXXXAPXXG KAXGKNXXXT DXXXXGXQXX XHIPIXXXXX XXXXPXXXXX XGGXXXXXXX LLVIAQRHPA ICKIYPGHLI PLGSASFVQR RPITRLTNWD QHRTRTXXXX XXXXXXXXXX XXXXXKHGXX XXXXXXX

Validated NT Sequence

cnnnnnnnnn nnnnnnnnnn gnggnncagc catgtttnnn nnnctnnnan cnnnaannna nnannannnn nnnncannnn nnnctcannn nnnnggtcct ggttcgatgt tggtcccaat ttgtcaaccg ggtgattggc cgacgctgaa cgaagcttgc cgagccgaga ggaatgagat ggccaggata gattttgcag atggctggat gccgctgagc aataactagc aannncnngn ananannnnn nnnnccaccc cnannnnncn gnnnnnncgg nncgcnnnnn nnnngnaann tgnaggngat ggggatgtgg nnnnnntgct gcnngccnnn nnncanancg tccgtgangc ngangttctt tccntccgct tttccnanga nnggagcnng gncgnccggt gcnattannn nnnnngnnnc nnnggccnna nntttgttga nnnnngngcc nnttcncgan ccnnnnntcg annntggngn natgnnnnnn natctgtnnn nnncnctngn nnnntgncnn nnnnnnatnt ccgnncatgc ncnnnntcnc atcngcncnc gcnantatat cngnnccgcn ccnatnncnt cnntatcgan gnntacngnc tgnnnctnat nnngnnnngn nnnnnncttt acanncnnnt nttgcggcna nnnnnaaant gntcannccc ttccttcnnt tttgcnnnct tcnattcntn annnnacncc tatngnnnaa gncnncgctg gnangnngan ngc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLVPICQPG DWPTLNEACR AERNEMARID FADGWMPLTL PQLDFWEEFS FHPDQPVSTV AHCVEIEGDV DVEKLLRAIS QTVREAEVLS IRFSDEKEQG RPVQLCDPAH MPDVDFVDLS AFPDPRAEAG RRMETDLSAP LDLRRDRMSA HALYRIGARQ YIWYIRAHHI IIDGYGLALI EQRCGQLYSH FCGQGESGHP FHPFASFIHE EDAYRASPRW EADRKFWQDY LDGPVRLPIL DRGAEDYGEA GYHHDVAFRD DISIGLRAMA KATDIGWPDL LVLLSGLYLS RMLPMQGEDE GLTLWLPFMS RWGSVGAHMP GLLVNILPFH LEFDSGETLF SALRRNMDML KKHRLHGRYR IEQISADRGL VSGSRFFFSP LINVLPFSTP QFAGCRVARE VLANGPGDGF NLTYRADVDG AGLTLKIEAA SNMMDAQTFA RIAAGLPAFL KASLAPHLIE RPVSDLRRRL P

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggtccca atttgtcaac cgggtgattg gccgacgctg aacgaagctt gccgagccga gaggaatgag atggccagga tagattttgc agatggctgg atgccgctga cattgcccca gctcgatttc tgggaagagt tttccttcca ccccgaccag ccggtctcca cggtcgcgca ttgcgtggaa atcgaaggcg atgtggatgt ggaaaaattg ctgcgcgcca tttcgcagac cgtccgtgag gcggaggttc tttccatccg cttttccgat gaaaaggagc aggggcgtcc ggtgcaatta tgcgacccgg cgcatatgcc ggacgtggat tttgttgatc tcagcgcctt tcccgatccg cgggcagagg ctgggcggcg tatggaaacc gatctgtccg caccgcttga tctgcgccgg gacaggatgt ccgcccatgc gctttatcgc atcggcgcgc gccaatatat ctggtatatc cgcgcccatc acatcattat cgacggttac gggctggcgc tgatcgaaca gcgttgcggc cagctttaca gccatttttg cggccagggc gaaagtggcc accccttcca tccttttgca agcttcattc atgaggagga cgcctatcgg gcaagccccc gctgggaggc cgaccgcaaa ttctggcagg attatctgga cgggcctgtg cgccttccca tcctcgatcg gggcgcggaa gattatggcg aggcgggcta tcatcatgac gtcgcctttc gcgacgatat ttccataggc cttcgcgcga tggcaaaagc cacggatatc ggctggcccg atcttctggt gcttttatcg gggctctatc tctcgcggat gcttccgatg cagggagagg acgaggggct gacgctgtgg ctgcctttca tgagccgctg gggcagcgtg ggcgcacata tgccggggct tctggtcaat atcctgccgt tccatcttga attcgattcc ggcgaaacat tgttctctgc gttacggcgc aatatggaca tgctgaaaaa gcaccggctg cacgggcgct atcgcatcga acagatttcc gccgaccgcg gcctcgtcag tggaagccgc tttttcttct cgccgctgat taatgttctg ccattcagca cgccgcaatt tgcaggttgc cgggttgcgc gtgaggttct ggccaatggc cccggcgacg gcttcaacct gacctatcgt gccgatgtcg atggcgctgg ccttaccttg aaaatcgaag cggcatcgaa catgatggat gcgcaaacct ttgcccgcat tgcggcgggc cttccggcct ttctcaaggc aagccttgcg ccccatctca tcgagcgtcc ggtgagcgat cttcgccgcc gtttgccata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for BrabA.17411.a.A1.PW31542

PURIFICATION DATe:	6/3/2011
CONCENTRATION:	21.01mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	18
VIAL VOLUME:	200µl
PERCENT IDENTITY:	15
PERCENT COVERAGE:	38

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGGTCCCA ATTTGTCAAC CGGGTGATTG GCCGACGCTG AACGAAGCTT GCCGAGCCGA GAGGAATGAG ATGGCCAGGA TAGATTTTGC AGATGGCTGG ATGCCGCTGA CATTGCCCCA GCTCGATTTC TGGGAAGAGT TTTCCTTCCA CCCCGACCAG CCGGTCTCCA CGGTCGCGCA TTGCGTGGAA ATCGAAGGCG ATGTGGATGT GGAAAAATTG CTGCGCGCCA TTTCGCAGAC CGTCCGTGAG GCGGAGGTTC TTTCCATCCG CTTTTCCGAT GAAAAGGAGC AGGGGCGTCC GGTGCAATTA TGCGACCCGG CGCATATGCC GGACGTGGAT TTTGTTGATC TCAGCGCCTT TCCCGATCCG CGGGCAGAGG CTGGGCGGCG TATGGAAACC GATCTGTCCG CACCGCTTGA TCTGCGCCGG GACAGGATGT CCGCCCATGC GCTTTATCGC ATCGGCGCGC GCCAATATAT CTGGTATATC CGCGCCCATC ACATCATTAT CGACGGTTAC GGGCTGGCGC TGATCGAACA GCGTTGCGGC CAGCTTTACA GCCATTTTTG CGGCCAGGGC GAAAGTGGCC ACCCCTTCCA TCCTTTTGCA AGCTTCATTC ATGAGGAGGA CGCCTATCGG GCAAGCCCCC GCTGGGAGGC CGACCGCAAA TTCTGGCAGG ATTATCTGGA CGGGCCTGTG CGCCTTCCCA TCCTCGATCG GGGCGCGGAA GATTATGGCG AGGCGGGCTA TCATCATGAC GTCGCCTTTC GCGACGATAT TTCCATAGGC CTTCGCGCGA TGGCAAAAGC CACGGATATC GGCTGGCCCG ATCTTCTGGT GCTTTTATCG GGGCTCTATC TCTCGCGGAT GCTTCCGATG CAGGGAGAGG ACGAGGGGCT GACGCTGTGG CTGCCTTTCA TGAGCCGCTG GGGCAGCGTG GGCGCACATA TGCCGGGGCT TCTGGTCAAT ATCCTGCCGT TCCATCTTGA ATTCGATTCC GGCGAAACAT TGTTCTCTGC GTTACGGCGC AATATGGACA TGCTGAAAAA GCACCGGCTG CACGGGCGCT ATCGCATCGA ACAGATTTCC GCCGACCGCG GCCTCGTCAG TGGAAGCCGC TTTTTCTTCT CGCCGCTGAT TAATGTTCTG CCATTCAGCA CGCCGCAATT TGCAGGTTGC CGGGTTGCGC GTGAGGTTCT GGCCAATGGC CCCGGCGACG GCTTCAACCT GACCTATCGT GCCGATGTCG ATGGCGCTGG CCTTACCTTG AAAATCGAAG CGGCATCGAA CATGATGGAT GCGCAAACCT TTGCCCGCAT TGCGGCGGGC CTTCCGGCCT TTCTCAAGGC AAGCCTTGCG CCCCATCTCA TCGAGCGTCC GGTGAGCGAT CTTCGCCGCC GTTTGCCATA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A