BrabA.17403.a
Ribosomal-protein-alanine N-acetyltransferase
| CENTER ID: | BrabA.17403.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.17403.a.A1.GE31637 | Full length( BrabA.17403.a ) | 1 | 225 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.17403.a.A1.PS01078 | Full length( BrabA.17403.a ) | 1 | 225 | ||
| BrabA.17403.a.A1.PS01079 | Full length( BrabA.17403.a ) | 1 | 225 |
Structures
3TCV
DEPOSITED: 8/9/2011
DETERMINATION: XRay
CLONE: BrabA.17403.a.A1.GE31637
PROTEIN: BrabA.17403.a.A1.PS01078
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_133138 |
| PATRIC ID: | fig|359391.4.peg.2367 |
| RefSeq: | YP_418335.1 |
| UniProt: | Q2YIN4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDLQNWTPR PKPERKIFEG RYVRLEPLNA QKHGDELFAA SSVEDAEQRF TWLFETPPAT RAEFEPWLDK ASKSDDPLFF AVIDKASGKV AGRQALMRID PANGVIEIGS IYWGPLISRR PAATEAQFLF MQYVFDVLGY RRYEWECHNE NGPSRRAAER FGFRFEGIFR QHMVVKGRNR DTAWFSVLDS EWPALKQAYQ AWLAPENFDS AGQQKKTLQE FRDLG
NT Sequence
ATGACTGATT TGCAGAATTG GACACCGCGT CCAAAGCCGG AGCGCAAGAT ATTTGAAGGC CGCTATGTGC GCCTTGAACC GCTGAACGCG CAAAAGCATG GCGACGAGCT TTTTGCAGCC TCTTCGGTGG AAGATGCGGA GCAGCGCTTT ACATGGCTGT TTGAAACTCC GCCTGCCACA CGCGCCGAAT TCGAGCCATG GCTGGACAAG GCTTCCAAAA GCGATGATCC GCTGTTTTTC GCGGTGATCG ACAAGGCAAG CGGCAAAGTG GCCGGTCGGC AGGCGCTGAT GCGCATCGAC CCGGCAAATG GCGTGATCGA GATCGGGAGC ATCTATTGGG GGCCGCTCAT CTCGCGCAGG CCTGCGGCAA CCGAGGCGCA GTTCCTGTTC ATGCAATATG TTTTCGATGT GCTTGGCTAT CGTCGCTATG AATGGGAATG CCACAATGAA AATGGGCCTT CCAGGCGTGC TGCCGAGCGT TTCGGCTTCC GGTTTGAGGG CATTTTCCGC CAGCATATGG TGGTCAAGGG CAGGAACCGG GATACGGCGT GGTTCTCCGT TCTCGACAGC GAATGGCCTG CCTTGAAACA AGCCTATCAG GCATGGCTTG CACCGGAAAA TTTTGATAGT GCTGGCCAGC AAAAGAAGAC GTTGCAGGAG TTTCGTGATC TTGGC
Details for BrabA.17403.a.A1.GE31637
| HARVESTED ON: | 3/1/2011 |
| SEQUENCED ON: | 3/7/2011 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDLQNWTP RPKPERKIFE GRYVRLEPLN AQKHGDELFA ASSVEDAEQR FTWLFETPPA TRAEFEPWLD KASKSDDPLF FAVIDKASGK VAGRQALMRI DPANGVIEIG SIYWGPLISR RPAATEAQFL FMQYVFDVLG YRRYEWECHN ENGPSRRAAE RFGFRFEGIF RQHMVVKGRN RDTAWFSVLD SEWPALKQAY QAWLAPENFD SAGQQKKTLQ EFRDLG
Validated NT Sequence
ttattagcca agatcacgaa actcctgcaa cgtcttcttt tgctggccag cactatcaaa attttccggt gcaagccatg cctgataggc ttgtttcaag gcaggccatt cgctgtcgag aacggagaac cacgccgtat cccggttcct gcccttgacc accatatgct ggcggaaaat gccctcaaac cggaagccga aacgctcggc agcacgcctg gaaggcccat tttcattgtg gcattcccat tcatagcgac gatagccaag cacatcgaaa acatattgca tgaacaggaa ctgcgcctcg gttgccgcag gcctgcgcga gatgagcggc ccccaataga tgctcccgat ctcgatcacg ccatttgccg ggtcgatgcg catcagcgcc tgccgaccgg ccactttgcc gcttgccttg tcgatcaccg cgaaaaacag cggatcatcg cttttggaag ccttgtccag ccatggctcg aattcggcgc gtgtggcagg cggagtttca aacagccatg taaagcgctg ctccgcatct tccaccgaag aggctgcaaa aagctcgtcg ccatgctttt gcgcgttcag cggttcaagg cgcacatagc ggccttcaaa tatcttgcgc tccggctttg gacgcggtgt ccaattctgc aaatcagtca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccatggtata tctcc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDLQNWTP RPKPERKIFE GRYVRLEPLN AQKHGDELFA ASSVEDAEQR FTWLFETPPA TRAEFEPWLD KASKSDDPLF FAVIDKASGK VAGRQALMRI DPANGVIEIG SIYWGPLISR RPAATEAQFL FMQYVFDVLG YRRYEWECHN ENGPSRRAAE RFGFRFEGIF RQHMVVKGRN RDTAWFSVLD SEWPALKQAY QAWLAPENFD SAGQQKKTLQ EFRDLG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactgatttg cagaattgga caccgcgtcc aaagccggag cgcaagatat ttgaaggccg ctatgtgcgc cttgaaccgc tgaacgcgca aaagcatggc gacgagcttt ttgcagcctc ttcggtggaa gatgcggagc agcgctttac atggctgttt gaaactccgc ctgccacacg cgccgaattc gagccatggc tggacaaggc ttccaaaagc gatgatccgc tgtttttcgc ggtgatcgac aaggcaagcg gcaaagtggc cggtcggcag gcgctgatgc gcatcgaccc ggcaaatggc gtgatcgaga tcgggagcat ctattggggg ccgctcatct cgcgcaggcc tgcggcaacc gaggcgcagt tcctgttcat gcaatatgtt ttcgatgtgc ttggctatcg tcgctatgaa tgggaatgcc acaatgaaaa tgggccttcc aggcgtgctg ccgagcgttt cggcttccgg tttgagggca ttttccgcca gcatatggtg gtcaagggca ggaaccggga tacggcgtgg ttctccgttc tcgacagcga atggcctgcc ttgaaacaag cctatcaggc atggcttgca ccggaaaatt ttgatagtgc tggccagcaa aagaagacgt tgcaggagtt tcgtgatctt ggctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BrabA.17403.a.A1.PS01078
| PURIFICATION DATe: | 5/11/2011 |
| CONCENTRATION: | 28.7mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDLQNWTP RPKPERKIFE GRYVRLEPLN AQKHGDELFA ASSVEDAEQR FTWLFETPPA TRAEFEPWLD KASKSDDPLF FAVIDKASGK VAGRQALMRI DPANGVIEIG SIYWGPLISR RPAATEAQFL FMQYVFDVLG YRRYEWECHN ENGPSRRAAE RFGFRFEGIF RQHMVVKGRN RDTAWFSVLD SEWPALKQAY QAWLAPENFD SAGQQKKTLQ EFRDLG
Validated NT Sequence
ttattagcca agatcacgaa actcctgcaa cgtcttcttt tgctggccag cactatcaaa attttccggt gcaagccatg cctgataggc ttgtttcaag gcaggccatt cgctgtcgag aacggagaac cacgccgtat cccggttcct gcccttgacc accatatgct ggcggaaaat gccctcaaac cggaagccga aacgctcggc agcacgcctg gaaggcccat tttcattgtg gcattcccat tcatagcgac gatagccaag cacatcgaaa acatattgca tgaacaggaa ctgcgcctcg gttgccgcag gcctgcgcga gatgagcggc ccccaataga tgctcccgat ctcgatcacg ccatttgccg ggtcgatgcg catcagcgcc tgccgaccgg ccactttgcc gcttgccttg tcgatcaccg cgaaaaacag cggatcatcg cttttggaag ccttgtccag ccatggctcg aattcggcgc gtgtggcagg cggagtttca aacagccatg taaagcgctg ctccgcatct tccaccgaag aggctgcaaa aagctcgtcg ccatgctttt gcgcgttcag cggttcaagg cgcacatagc ggccttcaaa tatcttgcgc tccggctttg gacgcggtgt ccaattctgc aaatcagtca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccatggtata tctcc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDLQNWTP RPKPERKIFE GRYVRLEPLN AQKHGDELFA ASSVEDAEQR FTWLFETPPA TRAEFEPWLD KASKSDDPLF FAVIDKASGK VAGRQALMRI DPANGVIEIG SIYWGPLISR RPAATEAQFL FMQYVFDVLG YRRYEWECHN ENGPSRRAAE RFGFRFEGIF RQHMVVKGRN RDTAWFSVLD SEWPALKQAY QAWLAPENFD SAGQQKKTLQ EFRDLG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACTGATTTG CAGAATTGGA CACCGCGTCC AAAGCCGGAG CGCAAGATAT TTGAAGGCCG CTATGTGCGC CTTGAACCGC TGAACGCGCA AAAGCATGGC GACGAGCTTT TTGCAGCCTC TTCGGTGGAA GATGCGGAGC AGCGCTTTAC ATGGCTGTTT GAAACTCCGC CTGCCACACG CGCCGAATTC GAGCCATGGC TGGACAAGGC TTCCAAAAGC GATGATCCGC TGTTTTTCGC GGTGATCGAC AAGGCAAGCG GCAAAGTGGC CGGTCGGCAG GCGCTGATGC GCATCGACCC GGCAAATGGC GTGATCGAGA TCGGGAGCAT CTATTGGGGG CCGCTCATCT CGCGCAGGCC TGCGGCAACC GAGGCGCAGT TCCTGTTCAT GCAATATGTT TTCGATGTGC TTGGCTATCG TCGCTATGAA TGGGAATGCC ACAATGAAAA TGGGCCTTCC AGGCGTGCTG CCGAGCGTTT CGGCTTCCGG TTTGAGGGCA TTTTCCGCCA GCATATGGTG GTCAAGGGCA GGAACCGGGA TACGGCGTGG TTCTCCGTTC TCGACAGCGA ATGGCCTGCC TTGAAACAAG CCTATCAGGC ATGGCTTGCA CCGGAAAATT TTGATAGTGC TGGCCAGCAA AAGAAGACGT TGCAGGAGTT TCGTGATCTT GGCTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
Details for BrabA.17403.a.A1.PS01079
| PURIFICATION DATe: | 5/11/2011 |
| CONCENTRATION: | 46.5mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDLQNWTP RPKPERKIFE GRYVRLEPLN AQKHGDELFA ASSVEDAEQR FTWLFETPPA TRAEFEPWLD KASKSDDPLF FAVIDKASGK VAGRQALMRI DPANGVIEIG SIYWGPLISR RPAATEAQFL FMQYVFDVLG YRRYEWECHN ENGPSRRAAE RFGFRFEGIF RQHMVVKGRN RDTAWFSVLD SEWPALKQAY QAWLAPENFD SAGQQKKTLQ EFRDLG
Validated NT Sequence
ttattagcca agatcacgaa actcctgcaa cgtcttcttt tgctggccag cactatcaaa attttccggt gcaagccatg cctgataggc ttgtttcaag gcaggccatt cgctgtcgag aacggagaac cacgccgtat cccggttcct gcccttgacc accatatgct ggcggaaaat gccctcaaac cggaagccga aacgctcggc agcacgcctg gaaggcccat tttcattgtg gcattcccat tcatagcgac gatagccaag cacatcgaaa acatattgca tgaacaggaa ctgcgcctcg gttgccgcag gcctgcgcga gatgagcggc ccccaataga tgctcccgat ctcgatcacg ccatttgccg ggtcgatgcg catcagcgcc tgccgaccgg ccactttgcc gcttgccttg tcgatcaccg cgaaaaacag cggatcatcg cttttggaag ccttgtccag ccatggctcg aattcggcgc gtgtggcagg cggagtttca aacagccatg taaagcgctg ctccgcatct tccaccgaag aggctgcaaa aagctcgtcg ccatgctttt gcgcgttcag cggttcaagg cgcacatagc ggccttcaaa tatcttgcgc tccggctttg gacgcggtgt ccaattctgc aaatcagtca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccatggtata tctcc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDLQNWTP RPKPERKIFE GRYVRLEPLN AQKHGDELFA ASSVEDAEQR FTWLFETPPA TRAEFEPWLD KASKSDDPLF FAVIDKASGK VAGRQALMRI DPANGVIEIG SIYWGPLISR RPAATEAQFL FMQYVFDVLG YRRYEWECHN ENGPSRRAAE RFGFRFEGIF RQHMVVKGRN RDTAWFSVLD SEWPALKQAY QAWLAPENFD SAGQQKKTLQ EFRDLG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACTGATTTG CAGAATTGGA CACCGCGTCC AAAGCCGGAG CGCAAGATAT TTGAAGGCCG CTATGTGCGC CTTGAACCGC TGAACGCGCA AAAGCATGGC GACGAGCTTT TTGCAGCCTC TTCGGTGGAA GATGCGGAGC AGCGCTTTAC ATGGCTGTTT GAAACTCCGC CTGCCACACG CGCCGAATTC GAGCCATGGC TGGACAAGGC TTCCAAAAGC GATGATCCGC TGTTTTTCGC GGTGATCGAC AAGGCAAGCG GCAAAGTGGC CGGTCGGCAG GCGCTGATGC GCATCGACCC GGCAAATGGC GTGATCGAGA TCGGGAGCAT CTATTGGGGG CCGCTCATCT CGCGCAGGCC TGCGGCAACC GAGGCGCAGT TCCTGTTCAT GCAATATGTT TTCGATGTGC TTGGCTATCG TCGCTATGAA TGGGAATGCC ACAATGAAAA TGGGCCTTCC AGGCGTGCTG CCGAGCGTTT CGGCTTCCGG TTTGAGGGCA TTTTCCGCCA GCATATGGTG GTCAAGGGCA GGAACCGGGA TACGGCGTGG TTCTCCGTTC TCGACAGCGA ATGGCCTGCC TTGAAACAAG CCTATCAGGC ATGGCTTGCA CCGGAAAATT TTGATAGTGC TGGCCAGCAA AAGAAGACGT TGCAGGAGTT TCGTGATCTT GGCTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA