BrabA.17379.a
Amidohydrolase
| CENTER ID: | BrabA.17379.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.17379.a.A1.GE31630 | Full length( BrabA.17379.a ) | 1 | 454 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.17379.a.A1.PS01212 | Full length( BrabA.17379.a ) | 1 | 454 |
Structures
4F0L
DEPOSITED: 5/4/2012
DETERMINATION: XRay
CLONE: BrabA.17379.a.A1.GE31630
PROTEIN: BrabA.17379.a.A1.PS01212
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|359391.4.peg.2601 |
| RefSeq: | YP_418518.1 |
| UniProt: | Q2YIL4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDQSSQHFI HARQALLPDG WAENVRIGIA GGVICSIETG VLAGPDDERQ SVVVAGMANL HSHAFQYGMA GLAERRGPSA DSFWSWREIM YKFALTMTPE QAEAVALRLY VDMLEAGFTR VGEFHYLHHD CDGTPYANLS EMADRIAAAA TTAGMGLTLL PVFYAHSGFG GAAANEGQRR FINDPERFAR LIEGCRKTLE GFEGAVLGVA PHSLRAVTPD ELDSVTQLLP DAPVHIHVAE QVKEVEDCIA WSGKRPVEWL LDHQDVTARW CLIHATHMSD EETKHMAKAG AIAGLCPVTE ANLGDGTFNA TEFAAAGGKF GIGSDSNVLI GIGDELRQLE YSQRLYHRAR NVLAANEGST GRALFDGAVL GGNIAMGRPE DGLKKGASAD FVSLDVERLP HAKGDVVLDG WIFAGRAHVC DVWVRGVKQV EGGRHRLRDE AERAFQKALG ELLA
NT Sequence
ATGTCCGATC AATCCAGCCA GCATTTCATT CATGCCCGAC AGGCGCTGTT GCCTGACGGT TGGGCTGAAA ATGTGCGCAT CGGCATTGCA GGCGGTGTCA TTTGCTCCAT TGAGACAGGC GTTCTGGCCG GGCCGGACGA CGAGCGGCAG AGCGTCGTCG TTGCCGGTAT GGCCAACCTG CACAGCCACG CTTTTCAATA TGGCATGGCG GGGCTTGCAG AGCGGCGCGG CCCATCCGCC GATTCCTTCT GGAGCTGGCG CGAGATCATG TACAAATTCG CGCTCACCAT GACGCCTGAA CAGGCTGAAG CCGTGGCGCT GCGGCTCTAT GTCGATATGC TGGAAGCCGG GTTTACCCGC GTTGGCGAGT TTCATTATCT GCATCATGAT TGCGACGGCA CGCCTTATGC CAATCTTTCG GAAATGGCGG ATCGTATCGC GGCGGCGGCG ACAACGGCAG GCATGGGACT GACGCTTCTG CCGGTCTTTT ATGCGCATTC CGGTTTTGGC GGGGCCGCTG CCAATGAAGG CCAGCGGCGG TTCATCAATG ATCCTGAACG CTTCGCGCGC CTGATTGAGG GCTGCCGAAA AACGCTGGAG GGTTTTGAGG GCGCTGTTCT GGGTGTGGCG CCGCACAGTC TGCGCGCCGT GACGCCGGAT GAACTCGATA GTGTCACGCA GCTTCTGCCC GATGCGCCGG TTCATATTCA TGTGGCCGAA CAGGTAAAGG AAGTCGAGGA CTGTATCGCC TGGTCTGGCA AGCGCCCGGT CGAATGGCTT CTGGATCATC AGGATGTGAC GGCGCGCTGG TGTCTCATTC ACGCAACCCA TATGAGCGAC GAGGAAACGA AACATATGGC CAAGGCTGGT GCGATTGCCG GTCTTTGCCC GGTGACGGAG GCCAATCTCG GTGATGGCAC TTTCAATGCA ACCGAATTTG CTGCCGCTGG CGGCAAATTC GGCATAGGCT CGGATTCAAA CGTGCTGATC GGCATTGGCG ATGAACTGCG CCAGCTTGAA TATTCGCAGC GTCTTTATCA TCGCGCCCGC AATGTGCTGG CCGCAAATGA GGGATCGACG GGCCGTGCGT TGTTTGATGG TGCGGTTTTG GGCGGCAATA TCGCCATGGG GCGGCCGGAG GATGGCCTGA AAAAGGGAGC GTCCGCCGAT TTTGTTTCGC TTGATGTCGA GCGCCTGCCC CATGCGAAAG GCGATGTGGT GCTCGATGGC TGGATTTTTG CCGGTCGTGC GCATGTCTGT GATGTGTGGG TGCGGGGTGT CAAACAGGTG GAAGGTGGAC GTCACCGCTT GCGCGACGAA GCGGAGCGTG CTTTCCAGAA AGCCTTGGGC GAATTACTCG CC
Details for BrabA.17379.a.A1.GE31630
| HARVESTED ON: | 3/1/2011 |
| SEQUENCED ON: | 3/7/2011 |
| EXPECTED MW: | 51kDa |
| OBSERVED MW: | 49kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 91 |
| PERCENT COVERAGE: | 84 |
Validated AA Sequence
SHVXXXXXXX TXXDXXXXXX XXXXXPGSMS DQSSQHFIHA RQALLXDXWA XXVRIGIXGX VICXIEXXXX XXPDDXRXXV XVAXMXNLXX HAFXXGMXXL AXXRXPSADS FWSWREIMYK FALTMTPEQA EAVALRLYVD MLEAGFTRVX EFHYLHHDCD GXPYAXLSEM ADRIAAXATT AGMGLTLLPV FYXHSGFGGA AANEGQRRFI NDPERFARLI EGCRKTLEGF EGAVLGVAPH SLRAVTPDEL DSVTQLLPDA PVHIHVAEQV KEVEDCIAWS GKRPVEWLLD HQDVTARWCL IHATHMSDEE TKHMAKAGAI AGLCPVTEAN LGDGTFNATE FAAAGGKFGI GSDSNVLIGI GDELRQLEYS QRLYHRARNV LAAYEGSTXR ALFDGAVLGG NIAMGRPEDG LKKG
Validated NT Sequence
ctcccttttt caggccatcc tccggccgcc ccatggcgat attgccgccc aaaaccgcac catcaaacaa cgcacggncc gtcgatccct catatgcggc cagcacattg cgggcgcgat gataaagacg ctgcgaatat tcaagctggc gcagttcatc gccaatgccg atcagcacgt ttgaatccga gcctatgccg aatttgccgc cagcggcagc aaattcggtt gcattgaaag tgccatcacc gagattggcc tccgtcaccg ggcaaagacc ggcaatcgca ccagccttgg ccatatgttt cgtttcctcg tcgctcatat gggttgcgtg aatgagacac cagcgcgccg tcacatcctg atgatccaga agccattcga ccgggcgctt gccagaccag gcgatacagt cctcgacttc ctttacctgt tcggccacat gaatatgaac cggcgcatcg ggcagaagct gcgtgacact atcgagttca tccggcgtca cggcgcgcag actgtgcggc gccacaccca gaacagcgcc ctcaaaaccc tccagcgttt ttcggcagcc ctcaatcagg cgcgcgaagc gttcaggatc attgatgaac cgccgctggc cttcattggc agcggccccg ccaaaaccgg aatgngcata aaagaccggc agaagcgtca gtcccatgcc tgccgttgtc gccgncgccg cgatacgatc cgccatttcc gaaagatngg cataaggcgn gccgtcgcaa tcatgatgca gataatgaaa ctcgncaacg cgggtaaacc cggcttccag catatcgaca tagagccgca gcgccacggc ttcagcctgt tcaggcgtca tggtgagcgc gaatttgtac atgatctcgc gccagctcca gaaggaatcg gcggatgggn cgcgncnntn tgcaagcncc gncatgccat nntgaaaagc gtggcnnnnc aggttgncca tnccggcaac gncgacgntn tgccgntngt cgtccggncc gnccnnancg nnngtctcaa tngngcaaat gacnccgccn gcaatgccga tgcgcacann ntcagcccan nngtcngnca acagcgcctg tcgggcatga atgaaatgct ggctggattg atcggacatc gaaccaggan cnntnnnttn naannntnnt nnnnnnnnnt tgtcnnnnnt tgtnnntnnn nnnngagngn nntaaacatg gctg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDQSSQHF IHARQALLPD GWAENVRIGI AGGVICSIET GVLAGPDDER QSVVVAGMAN LHSHAFQYGM AGLAERRGPS ADSFWSWREI MYKFALTMTP EQAEAVALRL YVDMLEAGFT RVGEFHYLHH DCDGTPYANL SEMADRIAAA ATTAGMGLTL LPVFYAHSGF GGAAANEGQR RFINDPERFA RLIEGCRKTL EGFEGAVLGV APHSLRAVTP DELDSVTQLL PDAPVHIHVA EQVKEVEDCI AWSGKRPVEW LLDHQDVTAR WCLIHATHMS DEETKHMAKA GAIAGLCPVT EANLGDGTFN ATEFAAAGGK FGIGSDSNVL IGIGDELRQL EYSQRLYHRA RNVLAANEGS TGRALFDGAV LGGNIAMGRP EDGLKKGASA DFVSLDVERL PHAKGDVVLD GWIFAGRAHV CDVWVRGVKQ VEGGRHRLRD EAERAFQKAL GELLA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccgatcaa tccagccagc atttcattca tgcccgacag gcgctgttgc ctgacggttg ggctgaaaat gtgcgcatcg gcattgcagg cggtgtcatt tgctccattg agacaggcgt tctggccggg ccggacgacg agcggcagag cgtcgtcgtt gccggtatgg ccaacctgca cagccacgct tttcaatatg gcatggcggg gcttgcagag cggcgcggcc catccgccga ttccttctgg agctggcgcg agatcatgta caaattcgcg ctcaccatga cgcctgaaca ggctgaagcc gtggcgctgc ggctctatgt cgatatgctg gaagccgggt ttacccgcgt tggcgagttt cattatctgc atcatgattg cgacggcacg ccttatgcca atctttcgga aatggcggat cgtatcgcgg cggcggcgac aacggcaggc atgggactga cgcttctgcc ggtcttttat gcgcattccg gttttggcgg ggccgctgcc aatgaaggcc agcggcggtt catcaatgat cctgaacgct tcgcgcgcct gattgagggc tgccgaaaaa cgctggaggg ttttgagggc gctgttctgg gtgtggcgcc gcacagtctg cgcgccgtga cgccggatga actcgatagt gtcacgcagc ttctgcccga tgcgccggtt catattcatg tggccgaaca ggtaaaggaa gtcgaggact gtatcgcctg gtctggcaag cgcccggtcg aatggcttct ggatcatcag gatgtgacgg cgcgctggtg tctcattcac gcaacccata tgagcgacga ggaaacgaaa catatggcca aggctggtgc gattgccggt ctttgcccgg tgacggaggc caatctcggt gatggcactt tcaatgcaac cgaatttgct gccgctggcg gcaaattcgg cataggctcg gattcaaacg tgctgatcgg cattggcgat gaactgcgcc agcttgaata ttcgcagcgt ctttatcatc gcgcccgcaa tgtgctggcc gcaaatgagg gatcgacggg ccgtgcgttg tttgatggtg cggttttggg cggcaatatc gccatggggc ggccggagga tggcctgaaa aagggagcgt ccgccgattt tgtttcgctt gatgtcgagc gcctgcccca tgcgaaaggc gatgtggtgc tcgatggctg gatttttgcc ggtcgtgcgc atgtctgtga tgtgtgggtg cggggtgtca aacaggtgga aggtggacgt caccgcttgc gcgacgaagc ggagcgtgct ttccagaaag ccttgggcga attactcgcc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BrabA.17379.a.A1.PS01212
| PURIFICATION DATe: | 9/22/2011 |
| CONCENTRATION: | 36.85mg/ml |
| OBSERVED MW: | 49kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 91 |
| PERCENT COVERAGE: | 84 |
Protocol Notes
notes unavailable
Validated AA Sequence
SHVXXXXXXX TXXDXXXXXX XXXXXPGSMS DQSSQHFIHA RQALLXDXWA XXVRIGIXGX VICXIEXXXX XXPDDXRXXV XVAXMXNLXX HAFXXGMXXL AXXRXPSADS FWSWREIMYK FALTMTPEQA EAVALRLYVD MLEAGFTRVX EFHYLHHDCD GXPYAXLSEM ADRIAAXATT AGMGLTLLPV FYXHSGFGGA AANEGQRRFI NDPERFARLI EGCRKTLEGF EGAVLGVAPH SLRAVTPDEL DSVTQLLPDA PVHIHVAEQV KEVEDCIAWS GKRPVEWLLD HQDVTARWCL IHATHMSDEE TKHMAKAGAI AGLCPVTEAN LGDGTFNATE FAAAGGKFGI GSDSNVLIGI GDELRQLEYS QRLYHRARNV LAAYEGSTXR ALFDGAVLGG NIAMGRPEDG LKKG
Validated NT Sequence
ctcccttttt caggccatcc tccggccgcc ccatggcgat attgccgccc aaaaccgcac catcaaacaa cgcacggncc gtcgatccct catatgcggc cagcacattg cgggcgcgat gataaagacg ctgcgaatat tcaagctggc gcagttcatc gccaatgccg atcagcacgt ttgaatccga gcctatgccg aatttgccgc cagcggcagc aaattcggtt gcattgaaag tgccatcacc gagattggcc tccgtcaccg ggcaaagacc ggcaatcgca ccagccttgg ccatatgttt cgtttcctcg tcgctcatat gggttgcgtg aatgagacac cagcgcgccg tcacatcctg atgatccaga agccattcga ccgggcgctt gccagaccag gcgatacagt cctcgacttc ctttacctgt tcggccacat gaatatgaac cggcgcatcg ggcagaagct gcgtgacact atcgagttca tccggcgtca cggcgcgcag actgtgcggc gccacaccca gaacagcgcc ctcaaaaccc tccagcgttt ttcggcagcc ctcaatcagg cgcgcgaagc gttcaggatc attgatgaac cgccgctggc cttcattggc agcggccccg ccaaaaccgg aatgngcata aaagaccggc agaagcgtca gtcccatgcc tgccgttgtc gccgncgccg cgatacgatc cgccatttcc gaaagatngg cataaggcgn gccgtcgcaa tcatgatgca gataatgaaa ctcgncaacg cgggtaaacc cggcttccag catatcgaca tagagccgca gcgccacggc ttcagcctgt tcaggcgtca tggtgagcgc gaatttgtac atgatctcgc gccagctcca gaaggaatcg gcggatgggn cgcgncnntn tgcaagcncc gncatgccat nntgaaaagc gtggcnnnnc aggttgncca tnccggcaac gncgacgntn tgccgntngt cgtccggncc gnccnnancg nnngtctcaa tngngcaaat gacnccgccn gcaatgccga tgcgcacann ntcagcccan nngtcngnca acagcgcctg tcgggcatga atgaaatgct ggctggattg atcggacatc gaaccaggan cnntnnnttn naannntnnt nnnnnnnnnt tgtcnnnnnt tgtnnntnnn nnnngagngn nntaaacatg gctg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDQSSQHF IHARQALLPD GWAENVRIGI AGGVICSIET GVLAGPDDER QSVVVAGMAN LHSHAFQYGM AGLAERRGPS ADSFWSWREI MYKFALTMTP EQAEAVALRL YVDMLEAGFT RVGEFHYLHH DCDGTPYANL SEMADRIAAA ATTAGMGLTL LPVFYAHSGF GGAAANEGQR RFINDPERFA RLIEGCRKTL EGFEGAVLGV APHSLRAVTP DELDSVTQLL PDAPVHIHVA EQVKEVEDCI AWSGKRPVEW LLDHQDVTAR WCLIHATHMS DEETKHMAKA GAIAGLCPVT EANLGDGTFN ATEFAAAGGK FGIGSDSNVL IGIGDELRQL EYSQRLYHRA RNVLAANEGS TGRALFDGAV LGGNIAMGRP EDGLKKGASA DFVSLDVERL PHAKGDVVLD GWIFAGRAHV CDVWVRGVKQ VEGGRHRLRD EAERAFQKAL GELLA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCGATCAA TCCAGCCAGC ATTTCATTCA TGCCCGACAG GCGCTGTTGC CTGACGGTTG GGCTGAAAAT GTGCGCATCG GCATTGCAGG CGGTGTCATT TGCTCCATTG AGACAGGCGT TCTGGCCGGG CCGGACGACG AGCGGCAGAG CGTCGTCGTT GCCGGTATGG CCAACCTGCA CAGCCACGCT TTTCAATATG GCATGGCGGG GCTTGCAGAG CGGCGCGGCC CATCCGCCGA TTCCTTCTGG AGCTGGCGCG AGATCATGTA CAAATTCGCG CTCACCATGA CGCCTGAACA GGCTGAAGCC GTGGCGCTGC GGCTCTATGT CGATATGCTG GAAGCCGGGT TTACCCGCGT TGGCGAGTTT CATTATCTGC ATCATGATTG CGACGGCACG CCTTATGCCA ATCTTTCGGA AATGGCGGAT CGTATCGCGG CGGCGGCGAC AACGGCAGGC ATGGGACTGA CGCTTCTGCC GGTCTTTTAT GCGCATTCCG GTTTTGGCGG GGCCGCTGCC AATGAAGGCC AGCGGCGGTT CATCAATGAT CCTGAACGCT TCGCGCGCCT GATTGAGGGC TGCCGAAAAA CGCTGGAGGG TTTTGAGGGC GCTGTTCTGG GTGTGGCGCC GCACAGTCTG CGCGCCGTGA CGCCGGATGA ACTCGATAGT GTCACGCAGC TTCTGCCCGA TGCGCCGGTT CATATTCATG TGGCCGAACA GGTAAAGGAA GTCGAGGACT GTATCGCCTG GTCTGGCAAG CGCCCGGTCG AATGGCTTCT GGATCATCAG GATGTGACGG CGCGCTGGTG TCTCATTCAC GCAACCCATA TGAGCGACGA GGAAACGAAA CATATGGCCA AGGCTGGTGC GATTGCCGGT CTTTGCCCGG TGACGGAGGC CAATCTCGGT GATGGCACTT TCAATGCAAC CGAATTTGCT GCCGCTGGCG GCAAATTCGG CATAGGCTCG GATTCAAACG TGCTGATCGG CATTGGCGAT GAACTGCGCC AGCTTGAATA TTCGCAGCGT CTTTATCATC GCGCCCGCAA TGTGCTGGCC GCAAATGAGG GATCGACGGG CCGTGCGTTG TTTGATGGTG CGGTTTTGGG CGGCAATATC GCCATGGGGC GGCCGGAGGA TGGCCTGAAA AAGGGAGCGT CCGCCGATTT TGTTTCGCTT GATGTCGAGC GCCTGCCCCA TGCGAAAGGC GATGTGGTGC TCGATGGCTG GATTTTTGCC GGTCGTGCGC ATGTCTGTGA TGTGTGGGTG CGGGGTGTCA AACAGGTGGA AGGTGGACGT CACCGCTTGC GCGACGAAGC GGAGCGTGCT TTCCAGAAAG CCTTGGGCGA ATTACTCGCC TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA