SSGCID: Target Page

BrabA.17376.a

Flagellar basal body rod protein:Flagellar basal-body rod protein FlgC

CENTER ID:	BrabA.17376.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17376.a.A1.GE31627	Full length( BrabA.17376.a )		1	140

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.2428
RefSeq:	YP_418384.1
UniProt:	Q2YJ72

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSDALQSTL KIAASGLSAQ STRLRIVSEN IANAQSTART ADTDPYRRKT VSFRTELEQG TGAAEVRVAE VGKDTSPFIE QYDPSHPAAD ARGYVKYPNV NMVVEMADMR EANRSYEANL QVVRQARELI SMTIDLLRST

NT Sequence

ATGTCTTCCG ATGCCTTGCA AAGCACATTG AAGATCGCGG CATCGGGCCT TTCGGCCCAG TCGACACGCC TGCGCATCGT TTCCGAAAAT ATTGCCAATG CGCAGTCCAC CGCCAGGACG GCGGATACCG ATCCCTATCG CCGCAAGACG GTTTCGTTCC GCACCGAGCT TGAACAAGGC ACGGGTGCAG CCGAGGTTCG GGTTGCGGAA GTGGGCAAGG ATACCTCGCC CTTTATCGAA CAATATGATC CGAGCCATCC CGCAGCGGAT GCGCGCGGCT ATGTCAAATA TCCAAATGTC AACATGGTCG TCGAGATGGC GGACATGCGC GAGGCAAACC GTTCCTACGA AGCCAATTTG CAGGTGGTCA GGCAGGCGCG TGAGCTGATT TCGATGACGA TCGACCTCTT GAGGAGCACA

Details for BrabA.17376.a.A1.GE31627

HARVESTED ON:	3/1/2011
SEQUENCED ON:	3/7/2011
EXPECTED MW:	17kDa
OBSERVED MW:	17kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSSDALQST LKIAASGLSA QSTRLRIVSE NIANAQSTAR TADTDPYRRK TVSFRTELEQ GTGAAEVRVA EVGKDTSPFI EQYDPSHPAA DARGYVKYPN VNMVVEMADM REANRSYEAN LQVVRQAREL ISMTIDLLRS T

Validated NT Sequence

attttgttta nctttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgtct tccgatgcct tgcaaagcac attgaagatc gcggcatcgg gcctttcggc ccagtcgaca cgcctgcgca tcgtttccga aaatattgcc aatgcgcagt ccaccgccag gacggcggat accgatccct atcgccgcaa gacggtttcg ttccgcaccg agcttgaaca aggcacgggt gcagccgagg ttcgggttgc ggaagtgggc aaggatacct cgccctttat cgaacaatat gatccgagcc atcccgcagc ggatgcgcgc ggctatgtca aatatccaaa tgtcaacatg gtcgtcgaga tggcggacat gcgcgaggca aaccgttcct acgaagccaa tttgcaggtg gtcaggcagg cgcgtgagct gatttcgatg acgatcgacc tcttgaggag cacataataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccnnn nnnnnnnnnn nnnnnnna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSSDALQST LKIAASGLSA QSTRLRIVSE NIANAQSTAR TADTDPYRRK TVSFRTELEQ GTGAAEVRVA EVGKDTSPFI EQYDPSHPAA DARGYVKYPN VNMVVEMADM REANRSYEAN LQVVRQAREL ISMTIDLLRS T

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcttccgat gccttgcaaa gcacattgaa gatcgcggca tcgggccttt cggcccagtc gacacgcctg cgcatcgttt ccgaaaatat tgccaatgcg cagtccaccg ccaggacggc ggataccgat ccctatcgcc gcaagacggt ttcgttccgc accgagcttg aacaaggcac gggtgcagcc gaggttcggg ttgcggaagt gggcaaggat acctcgccct ttatcgaaca atatgatccg agccatcccg cagcggatgc gcgcggctat gtcaaatatc caaatgtcaa catggtcgtc gagatggcgg acatgcgcga ggcaaaccgt tcctacgaag ccaatttgca ggtggtcagg caggcgcgtg agctgatttc gatgacgatc gacctcttga ggagcacata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a