SSGCID: Target Page

BrabA.17375.a

Spermidine/putrescine-binding periplasmic protein

CENTER ID:	BrabA.17375.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17375.a.A2.GE31626	Mature protein( BrabA.17375.a )		25	349

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17375.a.A2.PS01089	Mature protein( BrabA.17375.a )		25	349

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.3363
RefSeq:	YP_419195.1
UniProt:	Q2YJI9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKKIKTMTV LAAVLLAGGA LAAKADSIVY ATTGGQMRQT LERAFYQPFA KESGTDVIPY DIEVPDQWAR VEAMKRAGKY DFDIVTATGP DLVDKADLLL TIDCSKLPNV EAKGLEGSCK EKGVARTTGG MLLAYDASVY KDKVPQTWAD FWDVKTFPGP RGLPDTGDRD WWLPAIALMA DGVPRDKIFP MDLDRAYKKL DEIKPYISVW WKTGNQVAQI MRDQEVAMTM SYSGRALTAM NEGAPFQLSW NDAIMDTGYF AILKDAPNPE GALKFINYFY GHAEGHPAFI KTANYATHSK DGMALLPPEE QKNYATAPEN FKLLMVPDFK WIGENRDMLR ERWQNWLAE

NT Sequence

ATGTCCAAGA AAATAAAAAC GATGACTGTG CTGGCCGCGG TTCTGCTGGC TGGCGGTGCG CTTGCCGCAA AAGCCGATAG CATCGTCTAC GCCACGACTG GCGGACAGAT GCGCCAGACG CTTGAACGCG CATTCTACCA GCCTTTTGCA AAGGAAAGCG GCACGGACGT CATCCCATAT GACATCGAAG TGCCCGACCA GTGGGCCCGT GTCGAAGCGA TGAAGCGGGC CGGAAAATAC GATTTCGATA TCGTTACGGC AACCGGCCCG GACCTGGTCG ACAAGGCCGA CCTTCTTTTG ACCATCGATT GTTCGAAACT GCCCAATGTG GAAGCGAAGG GCCTTGAGGG TTCCTGCAAG GAAAAAGGCG TGGCCCGGAC CACCGGCGGC ATGCTGCTCG CCTATGATGC ATCCGTGTAT AAAGACAAGG TTCCGCAAAC ATGGGCTGAT TTTTGGGATG TGAAGACATT TCCCGGCCCG CGTGGCCTGC CCGATACCGG CGATCGCGAC TGGTGGCTGC CGGCCATTGC CTTGATGGCG GACGGCGTAC CCCGCGACAA GATTTTTCCG ATGGACCTTG ATCGCGCCTA CAAGAAGCTC GATGAGATCA AGCCCTATAT CAGCGTGTGG TGGAAGACCG GCAATCAGGT TGCGCAGATC ATGCGCGATC AGGAAGTGGC TATGACCATG TCCTATTCCG GGCGCGCCTT GACCGCGATG AACGAAGGCG CTCCCTTCCA GCTGAGCTGG AACGACGCCA TCATGGATAC GGGATATTTC GCCATTCTCA AGGATGCGCC AAATCCGGAA GGCGCCCTGA AATTCATCAA TTATTTCTAC GGTCACGCCG AAGGGCACCC GGCCTTCATC AAGACTGCAA ACTATGCGAC CCATTCCAAG GATGGTATGG CGTTGCTTCC GCCGGAAGAA CAGAAGAATT ATGCGACGGC GCCTGAGAAT TTCAAACTGC TGATGGTGCC GGACTTCAAG TGGATCGGTG AAAACCGCGA CATGCTTCGC GAACGCTGGC AAAACTGGCT GGCCGAA

Details for BrabA.17375.a.A2.GE31626

HARVESTED ON:	3/1/2011
SEQUENCED ON:	3/7/2011
EXPECTED MW:	39kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	94
PERCENT COVERAGE:	69

Validated AA Sequence

MWKRRALRVP ARKKAWPRTT GGMLLAYDAS VYKDKVPQTW ADFWDVKTFP GPRGLPDTGD RDWWLPAIAL MADGVPRDKI FPMDLDRAYK KLDEIKPYIS VWWKTGNQVA QIMRDQEVAM TMSYSGRALT AMNEGAPFQL SWNDAIMDTG YFAILKDAPN PEGALKFINY FYGHAEGHPA FIKTANYATH SKDGMALLPP EEQKNYATAP ENFKLLMVPD FKWIGENRDM LRERWQNWLA

Validated NT Sequence

ttcggccagc cagttttgcc agcgttcgcg aagcatgtcg cggttttcac cgatccactt gaagtccggc accatcagca gtttgaaatt ctcaggcgcc gtcgcataat tcttctgttc ttccggcgga agcaacgcca taccatcctt ggaatgggtc gcatagtttg cagtcttgat gaaggccggg tgcccttcgg cgtgaccgta gaaataattg atgaatttca gggcgccttc cggatttggc gcatccttga gaatggcgaa atatcccgta tccatgatgg cgtcgttcca gctcagctgg aagggagcgc cttcgttcat cgcggtcaag gcgcgcccgg aataggacat ggtcatagcc acttcctgat cgcgcatgat ctgcgcaacc tgattgccgg tcttccacca cacgctgata tagggcttga tctcatcgag cttcttgtag gcgcgatcaa ggtccatcgg aaaaatcttg tcgcggggta cgccgtccgc catcaaggca atggccggca gccaccagtc gcgatcgccg gtatcgggca ggccacgcgg gccgggaaat gtcttcacat cccaaaaatc agcccatgtt tgcggaacct tgtctttata cacggatgca tcataggcga gcagcatgcc gccggtggtc cggggccacg cctttttcct tgcaggaacc ctcaaggccc ttcgcttcca cattgggcag tttcgaacaa tcgatggtca aaagaaggtc ggccttgtcg accaggtccg ggccggttgc cgtaacgata tcgaaatcgt attttccggc ccgcttcatc gcttcnacac gggcccactg gtcgggcact tcgatgtcat atgggatgac gtc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMADSIVYAT TGGQMRQTLE RAFYQPFAKE SGTDVIPYDI EVPDQWARVE AMKRAGKYDF DIVTATGPDL VDKADLLLTI DCSKLPNVEA KGLEGSCKEK GVARTTGGML LAYDASVYKD KVPQTWADFW DVKTFPGPRG LPDTGDRDWW LPAIALMADG VPRDKIFPMD LDRAYKKLDE IKPYISVWWK TGNQVAQIMR DQEVAMTMSY SGRALTAMNE GAPFQLSWND AIMDTGYFAI LKDAPNPEGA LKFINYFYGH AEGHPAFIKT ANYATHSKDG MALLPPEEQK NYATAPENFK LLMVPDFKWI GENRDMLRER WQNWLAE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgatagc atcgtctacg ccacgactgg cggacagatg cgccagacgc ttgaacgcgc attctaccag ccttttgcaa aggaaagcgg cacggacgtc atcccatatg acatcgaagt gcccgaccag tgggcccgtg tcgaagcgat gaagcgggcc ggaaaatacg atttcgatat cgttacggca accggcccgg acctggtcga caaggccgac cttcttttga ccatcgattg ttcgaaactg cccaatgtgg aagcgaaggg ccttgagggt tcctgcaagg aaaaaggcgt ggcccggacc accggcggca tgctgctcgc ctatgatgca tccgtgtata aagacaaggt tccgcaaaca tgggctgatt tttgggatgt gaagacattt cccggcccgc gtggcctgcc cgataccggc gatcgcgact ggtggctgcc ggccattgcc ttgatggcgg acggcgtacc ccgcgacaag atttttccga tggaccttga tcgcgcctac aagaagctcg atgagatcaa gccctatatc agcgtgtggt ggaagaccgg caatcaggtt gcgcagatca tgcgcgatca ggaagtggct atgaccatgt cctattccgg gcgcgccttg accgcgatga acgaaggcgc tcccttccag ctgagctgga acgacgccat catggatacg ggatatttcg ccattctcaa ggatgcgcca aatccggaag gcgccctgaa attcatcaat tatttctacg gtcacgccga agggcacccg gccttcatca agactgcaaa ctatgcgacc cattccaagg atggtatggc gttgcttccg ccggaagaac agaagaatta tgcgacggcg cctgagaatt tcaaactgct gatggtgccg gacttcaagt ggatcggtga aaaccgcgac atgcttcgcg aacgctggca aaactggctg gccgaataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for BrabA.17375.a.A2.PS01089

PURIFICATION DATe:	5/29/2011
CONCENTRATION:	47.9mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	100µl
PERCENT IDENTITY:	94
PERCENT COVERAGE:	69

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGATAGC ATCGTCTACG CCACGACTGG CGGACAGATG CGCCAGACGC TTGAACGCGC ATTCTACCAG CCTTTTGCAA AGGAAAGCGG CACGGACGTC ATCCCATATG ACATCGAAGT GCCCGACCAG TGGGCCCGTG TCGAAGCGAT GAAGCGGGCC GGAAAATACG ATTTCGATAT CGTTACGGCA ACCGGCCCGG ACCTGGTCGA CAAGGCCGAC CTTCTTTTGA CCATCGATTG TTCGAAACTG CCCAATGTGG AAGCGAAGGG CCTTGAGGGT TCCTGCAAGG AAAAAGGCGT GGCCCGGACC ACCGGCGGCA TGCTGCTCGC CTATGATGCA TCCGTGTATA AAGACAAGGT TCCGCAAACA TGGGCTGATT TTTGGGATGT GAAGACATTT CCCGGCCCGC GTGGCCTGCC CGATACCGGC GATCGCGACT GGTGGCTGCC GGCCATTGCC TTGATGGCGG ACGGCGTACC CCGCGACAAG ATTTTTCCGA TGGACCTTGA TCGCGCCTAC AAGAAGCTCG ATGAGATCAA GCCCTATATC AGCGTGTGGT GGAAGACCGG CAATCAGGTT GCGCAGATCA TGCGCGATCA GGAAGTGGCT ATGACCATGT CCTATTCCGG GCGCGCCTTG ACCGCGATGA ACGAAGGCGC TCCCTTCCAG CTGAGCTGGA ACGACGCCAT CATGGATACG GGATATTTCG CCATTCTCAA GGATGCGCCA AATCCGGAAG GCGCCCTGAA ATTCATCAAT TATTTCTACG GTCACGCCGA AGGGCACCCG GCCTTCATCA AGACTGCAAA CTATGCGACC CATTCCAAGG ATGGTATGGC GTTGCTTCCG CCGGAAGAAC AGAAGAATTA TGCGACGGCG CCTGAGAATT TCAAACTGCT GATGGTGCCG GACTTCAAGT GGATCGGTGA AAACCGCGAC ATGCTTCGCG AACGCTGGCA AAACTGGCTG GCCGAATAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA