BrabA.17373.b

Leu/Ile/Val-binding protein homolog 7

CENTER ID: BrabA.17373.b
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.17373.b.A2.GE31623 Mature protein( BrabA.17373.b ) 22 399
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.17373.b.A2.PW31525 Mature protein( BrabA.17373.b ) 22 399

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.3116
RefSeq: YP_418976.1
UniProt: Q2YK58

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEKHLIALSV AALQAGAAPA SADIKMGSLY PFSGPLALLG DESARGLEIA VEEINAKGGV QGEKIVLVRG DAVDNNQAIG EARRLISVEN VAGIFGSFSS GRAVAASQVS ELAGVPYFEL GAVADEITDR GLENVYRANP YARDFAQMIV EMLQKKIAPK LGRDSKDLKI AVIYEDSSYG TSVAKHEETF LKEAGLNMVL SQSYPGNTVD MSSLVLDLKS AGADVVLQTS YQSDSVLFLQ QANEGGYKPS AIVGAGGGYS LQPTADAVGH DVIEAAYDVD FTQFAVNTSF TPGLEEFVEA YKKKYGETPR SGYSLTNYVG AKVILEALNK VKGFDAAAVK QALSAVDIEA GKTAMGYGFK FDQNNQNERA SMMGMQWQDG KLVTVYPDAA AISEIRLPQ
NT Sequence
TTGGAAAAAC ATCTTATCGC ATTATCTGTT GCAGCCTTGC AGGCAGGCGC CGCGCCTGCC TCGGCGGATA TCAAGATGGG GTCGCTTTAC CCGTTCAGCG GTCCGCTGGC GCTTCTGGGA GATGAAAGCG CGCGCGGTCT TGAAATAGCC GTGGAAGAGA TCAACGCCAA GGGTGGCGTA CAGGGCGAAA AAATCGTTCT GGTGCGAGGC GACGCGGTTG ATAACAATCA GGCGATCGGT GAAGCGCGTC GCCTGATCTC CGTTGAGAAT GTTGCCGGGA TTTTCGGGTC ATTTTCATCC GGTCGTGCGG TTGCCGCCAG CCAGGTGAGC GAACTGGCGG GCGTGCCTTA TTTCGAGCTA GGGGCGGTGG CCGACGAGAT CACCGATCGG GGACTGGAAA ACGTCTACCG CGCCAATCCC TATGCCCGTG ATTTTGCGCA GATGATTGTG GAGATGCTCC AGAAGAAGAT CGCGCCAAAG CTGGGCAGGG ATTCGAAAGA CCTCAAGATC GCGGTCATCT ATGAGGATTC GAGCTATGGC ACCTCCGTCG CCAAGCATGA GGAGACTTTC CTGAAGGAAG CTGGCCTCAA CATGGTGCTG TCGCAATCCT ATCCGGGCAA CACCGTCGAT ATGTCGTCTC TGGTTCTCGA CCTGAAGAGC GCCGGTGCGG ATGTGGTTCT GCAAACCTCC TATCAAAGCG ATTCAGTCCT GTTTCTGCAG CAGGCTAATG AAGGTGGCTA TAAGCCATCC GCAATCGTCG GTGCCGGCGG TGGATATTCG CTTCAGCCGA CTGCCGATGC CGTGGGGCAT GATGTGATTG AAGCAGCCTA TGATGTCGAT TTCACGCAAT TCGCCGTCAA TACGAGCTTT ACGCCCGGCC TTGAGGAGTT TGTCGAAGCC TACAAGAAAA AATATGGTGA AACACCGCGT TCAGGCTATT CGCTTACCAA TTATGTCGGT GCGAAAGTCA TTCTCGAAGC CTTGAACAAG GTTAAGGGAT TTGATGCGGC TGCGGTCAAG CAGGCCTTGA GCGCAGTCGA TATTGAAGCG GGCAAGACTG CGATGGGTTA CGGGTTCAAG TTTGACCAGA ACAACCAGAA TGAGCGTGCA TCCATGATGG GGATGCAATG GCAGGATGGC AAACTGGTCA CTGTCTACCC GGACGCCGCC GCCATATCGG AAATCCGGTT GCCGCAG
Details for BrabA.17373.b.A2.GE31623
HARVESTED ON: 3/1/2011
SEQUENCED ON: 3/7/2011
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 63
Validated AA Sequence
MIVEMLQKKI APKLGRDSKD LKIAVIYEDS SYGTSVAKHE ETFLKEAGLN MVLSQSYPGN TVDMSSLVLD LKSAGADVVL QTSYQSDSVL FLQQANEGGY KPSAIVGAGG GYSLQPTADA VGHDVIEAAY DVDFTQFAVN TSFTPGLEEF VEAYKKKYGE TPRSGYSLTN YVGAKVILEA LNKVKGFDAA AVKQALSAVD IEAGKTAMGY GFKFDQNNQN ERASMMGMQW QDGKLVTVYP DAAAISEIRL PQ
Validated NT Sequence
tactgcggca accggatttc cgatatggcg gcggcgtccg ggtagacagt gaccagtttg ccatcctgcc attgcatccc catcatggat gcacgctcat tctggttgtt ctggtcaaac ttgaacccgt aacccatcgc agtcttgccc gcttcaatat cgactgcgct caaggcctgc ttgaccgcag ccgcatcaaa tcccttaacc ttgttcaagg cttcgagaat gactttcgca ccgacataat tggtaagcga atagcctgaa cgcggtgttt caccatattt tttcttgtag gcttcgacaa actcctcaag gccgggcgta aagctcgtat tgacggcgaa ttgcgtgaaa tcgacatcat aggctgcttc aatcacatca tgccccacgg catcggcagt cggctgaagc gaatatccac cgccggcacc gacgattgcg gatggcttat agccaccttc attagcctgc tgcagaaaca ggactgaatc gctttgatag gaggtttgca gaaccacatc cgcaccggcg ctcttcaggt cgagaaccag agacgacata tcgacggtgt tgcccggata ggattgcgac agcaccatgt tgaggccagc ttccttcagg aaagtctcct catgcttggc gacggaggtg ccatagctcg aatcctcata gatgaccgcg atcttgaggt ctttcgaatc cctgcccagc tttggcgcga tcttcttctg gagcatctcc acaatcatct gcgcaaaatc acgggcatag ggattggcgc ggtagacgtt ttccagtccc cgatcggtga tctcgtcggc caccgcccct agctcgaaat aaggcacgcc cgccagttcg ctcacctggc tggcggcaac cgcacgaccg gatgaaaatg acccgaaaat cccggcaaca ttctcaacgg agatcaggcg acgcgcttca ccgatcgcct gattgtnatc anccgcgtcn cc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADIKMGSL YPFSGPLALL GDESARGLEI AVEEINAKGG VQGEKIVLVR GDAVDNNQAI GEARRLISVE NVAGIFGSFS SGRAVAASQV SELAGVPYFE LGAVADEITD RGLENVYRAN PYARDFAQMI VEMLQKKIAP KLGRDSKDLK IAVIYEDSSY GTSVAKHEET FLKEAGLNMV LSQSYPGNTV DMSSLVLDLK SAGADVVLQT SYQSDSVLFL QQANEGGYKP SAIVGAGGGY SLQPTADAVG HDVIEAAYDV DFTQFAVNTS FTPGLEEFVE AYKKKYGETP RSGYSLTNYV GAKVILEALN KVKGFDAAAV KQALSAVDIE AGKTAMGYGF KFDQNNQNER ASMMGMQWQD GKLVTVYPDA AAISEIRLPQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggatatc aagatggggt cgctttaccc gttcagcggt ccgctggcgc ttctgggaga tgaaagcgcg cgcggtcttg aaatagccgt ggaagagatc aacgccaagg gtggcgtaca gggcgaaaaa atcgttctgg tgcgaggcga cgcggttgat aacaatcagg cgatcggtga agcgcgtcgc ctgatctccg ttgagaatgt tgccgggatt ttcgggtcat tttcatccgg tcgtgcggtt gccgccagcc aggtgagcga actggcgggc gtgccttatt tcgagctagg ggcggtggcc gacgagatca ccgatcgggg actggaaaac gtctaccgcg ccaatcccta tgcccgtgat tttgcgcaga tgattgtgga gatgctccag aagaagatcg cgccaaagct gggcagggat tcgaaagacc tcaagatcgc ggtcatctat gaggattcga gctatggcac ctccgtcgcc aagcatgagg agactttcct gaaggaagct ggcctcaaca tggtgctgtc gcaatcctat ccgggcaaca ccgtcgatat gtcgtctctg gttctcgacc tgaagagcgc cggtgcggat gtggttctgc aaacctccta tcaaagcgat tcagtcctgt ttctgcagca ggctaatgaa ggtggctata agccatccgc aatcgtcggt gccggcggtg gatattcgct tcagccgact gccgatgccg tggggcatga tgtgattgaa gcagcctatg atgtcgattt cacgcaattc gccgtcaata cgagctttac gcccggcctt gaggagtttg tcgaagccta caagaaaaaa tatggtgaaa caccgcgttc aggctattcg cttaccaatt atgtcggtgc gaaagtcatt ctcgaagcct tgaacaaggt taagggattt gatgcggctg cggtcaagca ggccttgagc gcagtcgata ttgaagcggg caagactgcg atgggttacg ggttcaagtt tgaccagaac aaccagaatg agcgtgcatc catgatgggg atgcaatggc aggatggcaa actggtcact gtctacccgg acgccgccgc catatcggaa atccggttgc cgcagtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BrabA.17373.b.A2.PW31525
PURIFICATION DATe: 5/13/2011
CONCENTRATION: 38.14mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 63
Protocol Notes
notes unavailable
Validated AA Sequence
MIVEMLQKKI APKLGRDSKD LKIAVIYEDS SYGTSVAKHE ETFLKEAGLN MVLSQSYPGN TVDMSSLVLD LKSAGADVVL QTSYQSDSVL FLQQANEGGY KPSAIVGAGG GYSLQPTADA VGHDVIEAAY DVDFTQFAVN TSFTPGLEEF VEAYKKKYGE TPRSGYSLTN YVGAKVILEA LNKVKGFDAA AVKQALSAVD IEAGKTAMGY GFKFDQNNQN ERASMMGMQW QDGKLVTVYP DAAAISEIRL PQ
Validated NT Sequence
tactgcggca accggatttc cgatatggcg gcggcgtccg ggtagacagt gaccagtttg ccatcctgcc attgcatccc catcatggat gcacgctcat tctggttgtt ctggtcaaac ttgaacccgt aacccatcgc agtcttgccc gcttcaatat cgactgcgct caaggcctgc ttgaccgcag ccgcatcaaa tcccttaacc ttgttcaagg cttcgagaat gactttcgca ccgacataat tggtaagcga atagcctgaa cgcggtgttt caccatattt tttcttgtag gcttcgacaa actcctcaag gccgggcgta aagctcgtat tgacggcgaa ttgcgtgaaa tcgacatcat aggctgcttc aatcacatca tgccccacgg catcggcagt cggctgaagc gaatatccac cgccggcacc gacgattgcg gatggcttat agccaccttc attagcctgc tgcagaaaca ggactgaatc gctttgatag gaggtttgca gaaccacatc cgcaccggcg ctcttcaggt cgagaaccag agacgacata tcgacggtgt tgcccggata ggattgcgac agcaccatgt tgaggccagc ttccttcagg aaagtctcct catgcttggc gacggaggtg ccatagctcg aatcctcata gatgaccgcg atcttgaggt ctttcgaatc cctgcccagc tttggcgcga tcttcttctg gagcatctcc acaatcatct gcgcaaaatc acgggcatag ggattggcgc ggtagacgtt ttccagtccc cgatcggtga tctcgtcggc caccgcccct agctcgaaat aaggcacgcc cgccagttcg ctcacctggc tggcggcaac cgcacgaccg gatgaaaatg acccgaaaat cccggcaaca ttctcaacgg agatcaggcg acgcgcttca ccgatcgcct gattgtnatc anccgcgtcn cc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADIKMGSL YPFSGPLALL GDESARGLEI AVEEINAKGG VQGEKIVLVR GDAVDNNQAI GEARRLISVE NVAGIFGSFS SGRAVAASQV SELAGVPYFE LGAVADEITD RGLENVYRAN PYARDFAQMI VEMLQKKIAP KLGRDSKDLK IAVIYEDSSY GTSVAKHEET FLKEAGLNMV LSQSYPGNTV DMSSLVLDLK SAGADVVLQT SYQSDSVLFL QQANEGGYKP SAIVGAGGGY SLQPTADAVG HDVIEAAYDV DFTQFAVNTS FTPGLEEFVE AYKKKYGETP RSGYSLTNYV GAKVILEALN KVKGFDAAAV KQALSAVDIE AGKTAMGYGF KFDQNNQNER ASMMGMQWQD GKLVTVYPDA AAISEIRLPQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGGATATC AAGATGGGGT CGCTTTACCC GTTCAGCGGT CCGCTGGCGC TTCTGGGAGA TGAAAGCGCG CGCGGTCTTG AAATAGCCGT GGAAGAGATC AACGCCAAGG GTGGCGTACA GGGCGAAAAA ATCGTTCTGG TGCGAGGCGA CGCGGTTGAT AACAATCAGG CGATCGGTGA AGCGCGTCGC CTGATCTCCG TTGAGAATGT TGCCGGGATT TTCGGGTCAT TTTCATCCGG TCGTGCGGTT GCCGCCAGCC AGGTGAGCGA ACTGGCGGGC GTGCCTTATT TCGAGCTAGG GGCGGTGGCC GACGAGATCA CCGATCGGGG ACTGGAAAAC GTCTACCGCG CCAATCCCTA TGCCCGTGAT TTTGCGCAGA TGATTGTGGA GATGCTCCAG AAGAAGATCG CGCCAAAGCT GGGCAGGGAT TCGAAAGACC TCAAGATCGC GGTCATCTAT GAGGATTCGA GCTATGGCAC CTCCGTCGCC AAGCATGAGG AGACTTTCCT GAAGGAAGCT GGCCTCAACA TGGTGCTGTC GCAATCCTAT CCGGGCAACA CCGTCGATAT GTCGTCTCTG GTTCTCGACC TGAAGAGCGC CGGTGCGGAT GTGGTTCTGC AAACCTCCTA TCAAAGCGAT TCAGTCCTGT TTCTGCAGCA GGCTAATGAA GGTGGCTATA AGCCATCCGC AATCGTCGGT GCCGGCGGTG GATATTCGCT TCAGCCGACT GCCGATGCCG TGGGGCATGA TGTGATTGAA GCAGCCTATG ATGTCGATTT CACGCAATTC GCCGTCAATA CGAGCTTTAC GCCCGGCCTT GAGGAGTTTG TCGAAGCCTA CAAGAAAAAA TATGGTGAAA CACCGCGTTC AGGCTATTCG CTTACCAATT ATGTCGGTGC GAAAGTCATT CTCGAAGCCT TGAACAAGGT TAAGGGATTT GATGCGGCTG CGGTCAAGCA GGCCTTGAGC GCAGTCGATA TTGAAGCGGG CAAGACTGCG ATGGGTTACG GGTTCAAGTT TGACCAGAAC AACCAGAATG AGCGTGCATC CATGATGGGG ATGCAATGGC AGGATGGCAA ACTGGTCACT GTCTACCCGG ACGCCGCCGC CATATCGGAA ATCCGGTTGC CGCAGTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA