SSGCID: Target Page

BrabA.17373.a

Leu/Ile/Val-binding protein homolog 8

CENTER ID:	BrabA.17373.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17373.a.A2.GE31622	Mature protein( BrabA.17373.a )		26	403

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17373.a.A2.PS01095	Mature protein( BrabA.17373.a )		26	403

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.3124
RefSeq:	YP_418984.1
UniProt:	Q2YK50

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRLSRLLIGA SLGVALSSTV FTAALADVKF GSLYPISGSL ALLGEESARG LELAVDEVNA AGGIKGEKVV LERGDAVDNN QATGEARRLI SLVGVKAIFG TYSSARVIAA SQVSELAGIP YFEMGAVADE VTGRGLQYLF RTNPTAENMA KDSVDMLIKG IAPGLGKDPK DMKIGIIYED SSYGTSVAGH QEDNAKAAGL TVVLKSGYPS NTVDMSSLVL ELREKGADVV MQTSYQNDSV LFLQQANEAG YKPLAIIGGG GGYSMQPTAD AVGHDLIDGV FDADYTQYAV NTSATPGLTE FVEAYKAKYG SQPRSGHSLT NYVGAKAIFQ ALNAGEGFEP DQIVSAVKAL DIPDGQTAAG YGVKFGKNNQ NERATMMGMQ WQDGKLVTVY PENAAIAKMR FKK

NT Sequence

ATGAGACTGT CCAGGCTCCT TATCGGCGCA TCGCTCGGCG TTGCGCTTTC GTCCACTGTC TTCACCGCGG CACTCGCCGA TGTGAAATTC GGCTCGCTTT ATCCGATCAG CGGTTCGCTT GCGCTTCTGG GTGAAGAAAG CGCACGCGGT CTGGAACTTG CCGTAGACGA GGTCAATGCG GCTGGCGGCA TCAAGGGTGA AAAAGTCGTG CTTGAGCGCG GCGATGCCGT TGACAACAAC CAGGCTACCG GTGAGGCTCG CCGCCTGATC TCGCTTGTGG GTGTGAAGGC GATTTTCGGG ACTTATTCTT CGGCGCGGGT TATTGCCGCC TCGCAGGTGA GCGAGCTTGC AGGCATACCC TATTTCGAAA TGGGTGCCGT TGCCGACGAG GTGACGGGGC GTGGCTTGCA ATATCTTTTC CGCACCAATC CGACCGCGGA AAACATGGCC AAGGACTCTG TAGATATGCT CATCAAGGGC ATTGCTCCGG GCCTCGGCAA AGACCCCAAG GATATGAAAA TCGGCATTAT TTATGAGGAC TCTTCCTATG GCACATCCGT TGCCGGGCAT CAGGAAGATA ACGCAAAGGC AGCCGGTCTC ACTGTCGTTC TGAAATCCGG CTATCCATCC AACACAGTCG ACATGTCTTC GCTGGTTCTG GAGCTGCGCG AGAAGGGTGC GGATGTCGTC ATGCAGACGT CCTACCAGAA CGATTCCGTC CTGTTCCTGC AACAGGCCAA CGAAGCGGGC TATAAGCCCC TTGCCATCAT CGGCGGTGGC GGCGGTTATT CCATGCAGCC AACGGCGGAC GCCGTGGGAC ATGACCTGAT CGACGGCGTT TTCGATGCCG ACTATACGCA ATATGCAGTC AACACCTCCG CCACGCCGGG CCTGACCGAA TTTGTGGAAG CCTATAAGGC CAAATATGGC TCGCAACCGC GTTCCGGCCA TTCGCTGACC AATTATGTCG GGGCGAAGGC GATTTTTCAG GCTTTGAACG CTGGGGAAGG CTTTGAACCG GACCAGATCG TAAGCGCAGT CAAGGCACTG GACATTCCGG ACGGCCAGAC GGCAGCAGGC TATGGGGTCA AGTTTGGCAA GAACAACCAG AATGAGCGTG CGACGATGAT GGGGATGCAG TGGCAGGATG GCAAGCTCGT TACCGTCTAT CCGGAAAATG CCGCTATCGC CAAGATGCGC TTCAAGAAA

Details for BrabA.17373.a.A2.GE31622

HARVESTED ON:	3/1/2011
SEQUENCED ON:	3/7/2011
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	57

Validated AA Sequence

MADVKFGSLY PISGSLALLG EESARXLXLA VDEVNAAGGX XGEKVXLERG DAVDNNQATG EARRLISLVG VKAIFGTYSS ARVIAASQVS ELAGIPYFEM GAVADEVTGR GLQYLFRTNP TAENMAKDSV DMLIKGIAPG LGKDPKDMKI GIIYEDSSYG TSVAGHQEDN AKAAGLTVVL KSGYPSNTVD MSSLVLELRE KGADVVMQTS YQNDSVLFLQ QANEAGYK

Validated NT Sequence

gcttatagcc cgcttcgttg gcctgttgca ggaacaggac ggaatcgttc tggtaggacg tctgcatgac gacatccgca cccttctcgc gcagctccag aaccagcgaa gacatgtcga ctgtgttgga tggatagccg gatttcagaa cgacagtgag accggctgcc tttgcgttat cttcctgatg cccggcaacg gatgtgccat aggaagagtc ctcataaata atgccgattt tcatatcctt ggggtctttg ccgaggcccg gagcaatgcc cttgatgagc atatctacag agtccttggc catgttttcc gcggtcggat tggtgcggaa aagatattgc aagccacgcc ccgtcacctc gtcggcaacg gcacccattt cgaaataggg tatgcctgca agctcgctca cctgcgaggc ggcaataacc cgcgccgaag aataagtccc gaaaatcgcc ttcacaccca caagcgagat caggcggcga gcctcaccgg tagcctggtt gttgtcaacg gcatcgccgc gctcaagcnn gactttttca ccctngangc cgccagccgc attgacctcg tctacggcaa gttncagnnc gcgtgcgctt tcttcaccca gaagcgcaag cgaaccgctg atcggataaa gcgagccgaa tttcacatcg gccatcgaac caggacc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMADVKFGSL YPISGSLALL GEESARGLEL AVDEVNAAGG IKGEKVVLER GDAVDNNQAT GEARRLISLV GVKAIFGTYS SARVIAASQV SELAGIPYFE MGAVADEVTG RGLQYLFRTN PTAENMAKDS VDMLIKGIAP GLGKDPKDMK IGIIYEDSSY GTSVAGHQED NAKAAGLTVV LKSGYPSNTV DMSSLVLELR EKGADVVMQT SYQNDSVLFL QQANEAGYKP LAIIGGGGGY SMQPTADAVG HDLIDGVFDA DYTQYAVNTS ATPGLTEFVE AYKAKYGSQP RSGHSLTNYV GAKAIFQALN AGEGFEPDQI VSAVKALDIP DGQTAAGYGV KFGKNNQNER ATMMGMQWQD GKLVTVYPEN AAIAKMRFKK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgatgtg aaattcggct cgctttatcc gatcagcggt tcgcttgcgc ttctgggtga agaaagcgca cgcggtctgg aacttgccgt agacgaggtc aatgcggctg gcggcatcaa gggtgaaaaa gtcgtgcttg agcgcggcga tgccgttgac aacaaccagg ctaccggtga ggctcgccgc ctgatctcgc ttgtgggtgt gaaggcgatt ttcgggactt attcttcggc gcgggttatt gccgcctcgc aggtgagcga gcttgcaggc ataccctatt tcgaaatggg tgccgttgcc gacgaggtga cggggcgtgg cttgcaatat cttttccgca ccaatccgac cgcggaaaac atggccaagg actctgtaga tatgctcatc aagggcattg ctccgggcct cggcaaagac cccaaggata tgaaaatcgg cattatttat gaggactctt cctatggcac atccgttgcc gggcatcagg aagataacgc aaaggcagcc ggtctcactg tcgttctgaa atccggctat ccatccaaca cagtcgacat gtcttcgctg gttctggagc tgcgcgagaa gggtgcggat gtcgtcatgc agacgtccta ccagaacgat tccgtcctgt tcctgcaaca ggccaacgaa gcgggctata agccccttgc catcatcggc ggtggcggcg gttattccat gcagccaacg gcggacgccg tgggacatga cctgatcgac ggcgttttcg atgccgacta tacgcaatat gcagtcaaca cctccgccac gccgggcctg accgaatttg tggaagccta taaggccaaa tatggctcgc aaccgcgttc cggccattcg ctgaccaatt atgtcggggc gaaggcgatt tttcaggctt tgaacgctgg ggaaggcttt gaaccggacc agatcgtaag cgcagtcaag gcactggaca ttccggacgg ccagacggca gcaggctatg gggtcaagtt tggcaagaac aaccagaatg agcgtgcgac gatgatgggg atgcagtggc aggatggcaa gctcgttacc gtctatccgg aaaatgccgc tatcgccaag atgcgcttca agaaataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for BrabA.17373.a.A2.PS01095

PURIFICATION DATe:	5/26/2011
CONCENTRATION:	62.02mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	100µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	57

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGATGTG AAATTCGGCT CGCTTTATCC GATCAGCGGT TCGCTTGCGC TTCTGGGTGA AGAAAGCGCA CGCGGTCTGG AACTTGCCGT AGACGAGGTC AATGCGGCTG GCGGCATCAA GGGTGAAAAA GTCGTGCTTG AGCGCGGCGA TGCCGTTGAC AACAACCAGG CTACCGGTGA GGCTCGCCGC CTGATCTCGC TTGTGGGTGT GAAGGCGATT TTCGGGACTT ATTCTTCGGC GCGGGTTATT GCCGCCTCGC AGGTGAGCGA GCTTGCAGGC ATACCCTATT TCGAAATGGG TGCCGTTGCC GACGAGGTGA CGGGGCGTGG CTTGCAATAT CTTTTCCGCA CCAATCCGAC CGCGGAAAAC ATGGCCAAGG ACTCTGTAGA TATGCTCATC AAGGGCATTG CTCCGGGCCT CGGCAAAGAC CCCAAGGATA TGAAAATCGG CATTATTTAT GAGGACTCTT CCTATGGCAC ATCCGTTGCC GGGCATCAGG AAGATAACGC AAAGGCAGCC GGTCTCACTG TCGTTCTGAA ATCCGGCTAT CCATCCAACA CAGTCGACAT GTCTTCGCTG GTTCTGGAGC TGCGCGAGAA GGGTGCGGAT GTCGTCATGC AGACGTCCTA CCAGAACGAT TCCGTCCTGT TCCTGCAACA GGCCAACGAA GCGGGCTATA AGCCCCTTGC CATCATCGGC GGTGGCGGCG GTTATTCCAT GCAGCCAACG GCGGACGCCG TGGGACATGA CCTGATCGAC GGCGTTTTCG ATGCCGACTA TACGCAATAT GCAGTCAACA CCTCCGCCAC GCCGGGCCTG ACCGAATTTG TGGAAGCCTA TAAGGCCAAA TATGGCTCGC AACCGCGTTC CGGCCATTCG CTGACCAATT ATGTCGGGGC GAAGGCGATT TTTCAGGCTT TGAACGCTGG GGAAGGCTTT GAACCGGACC AGATCGTAAG CGCAGTCAAG GCACTGGACA TTCCGGACGG CCAGACGGCA GCAGGCTATG GGGTCAAGTT TGGCAAGAAC AACCAGAATG AGCGTGCGAC GATGATGGGG ATGCAGTGGC AGGATGGCAA GCTCGTTACC GTCTATCCGG AAAATGCCGC TATCGCCAAG ATGCGCTTCA AGAAATAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA