SSGCID: Target Page

BrabA.17372.a

Chromosome II, complete sequence, strain 2308

CENTER ID:	BrabA.17372.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17372.a.A1.GE31621	Full length( BrabA.17372.a )		1	155

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.2978
RefSeq:	YP_418857.1
UniProt:	Q2YKH7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDLGSVDVDE KASPHSADDR HQHYAVSKVV DDRPAPRRVE VLLGKERRRR WSADEKAEIT AASFVPGANI SAVARQYGVS QGLLHYWRRC AREHMSDDEE MRFVPVVRAD EGLGVPAAVT ALTIRVEING TCAVIEGSID EQALCMVFKA LRASA

NT Sequence

ATGGATTTGG GATCAGTAGA TGTCGACGAG AAGGCCTCAC CGCATAGTGC TGATGACAGA CATCAGCACT ATGCGGTGAG CAAAGTTGTC GATGATAGGC CGGCACCTCG CCGTGTCGAG GTTCTCCTCG GCAAAGAGCG CCGGCGCCGT TGGAGCGCTG ATGAGAAGGC GGAGATCACG GCGGCCAGCT TTGTGCCTGG AGCAAATATC TCTGCCGTTG CGCGCCAATA TGGCGTGAGC CAGGGGCTGC TGCACTATTG GCGGCGCTGC GCGCGAGAGC ACATGTCGGA TGACGAGGAG ATGCGCTTTG TGCCGGTTGT CAGAGCCGAT GAAGGCTTGG GCGTCCCGGC CGCGGTGACG GCGCTGACGA TCCGTGTCGA AATCAACGGC ACCTGTGCCG TGATCGAGGG TAGCATCGAT GAGCAGGCAC TGTGCATGGT GTTCAAAGCG CTCAGGGCCT CGGCG

Details for BrabA.17372.a.A1.GE31621

HARVESTED ON:	3/1/2011
SEQUENCED ON:	3/7/2011
EXPECTED MW:	19kDa
OBSERVED MW:	20kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDLGSVDVD EKASPHSADD RHQHYAVSKV VDDRPAPRRV EVLLGKERRR RWSADEKAEI TAASFVPGAN ISAVARQYGV SQGLLHYWRR CAREHMSDDE EMRFVPVVRA DEGLGVPAAV TALTIRVEIN GTCAVIEGSI DEQALCMVFK ALRASA

Validated NT Sequence

attttgtttn nctttaagaa ggaganatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggat ttgggatcag tagatgtcga cgagaaggcc tcaccgcata gtgctgatga cagacatcag cactatgcgg tgagcaaagt tgtcgatgat aggccggcac ctcgccgtgt cgaggttctc ctcggcaaag agcgccggcg ccgttggagc gctgatgaga aggcggagat cacggcggcc agctttgtgc ctggagcaaa tatctctgcc gttgcgcgcc aatatggcgt gagccagggg ctgctgcact attggcggcg ctgcgcgcga gagcacatgt cggatgacga ggagatgcgc tttgtgccgg ttgtcagagc cgatgaaggc ttgggcgtcc cggccgcggt gacggcgctg acgatccgtg tcgaaatcaa cggcacctgt gccgtgatcg agggtagcat cgatgagcag gcactgtgca tggtgttcaa agcgctcagg gcctcggcgt aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgct

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDLGSVDVD EKASPHSADD RHQHYAVSKV VDDRPAPRRV EVLLGKERRR RWSADEKAEI TAASFVPGAN ISAVARQYGV SQGLLHYWRR CAREHMSDDE EMRFVPVVRA DEGLGVPAAV TALTIRVEIN GTCAVIEGSI DEQALCMVFK ALRASA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatttggga tcagtagatg tcgacgagaa ggcctcaccg catagtgctg atgacagaca tcagcactat gcggtgagca aagttgtcga tgataggccg gcacctcgcc gtgtcgaggt tctcctcggc aaagagcgcc ggcgccgttg gagcgctgat gagaaggcgg agatcacggc ggccagcttt gtgcctggag caaatatctc tgccgttgcg cgccaatatg gcgtgagcca ggggctgctg cactattggc ggcgctgcgc gcgagagcac atgtcggatg acgaggagat gcgctttgtg ccggttgtca gagccgatga aggcttgggc gtcccggccg cggtgacggc gctgacgatc cgtgtcgaaa tcaacggcac ctgtgccgtg atcgagggta gcatcgatga gcaggcactg tgcatggtgt tcaaagcgct cagggcctcg gcgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa