SSGCID: Target Page

BrabA.17369.a

H+-transporting two-sector ATPase, alpha/beta subunit, central region:Disease resistance protein:ATP/GTP-binding site motif A...

CENTER ID:	BrabA.17369.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17369.a.A1.GE31618	Full length( BrabA.17369.a )		1	453

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17369.a.A1.PS01097	Full length( BrabA.17369.a )		1	453

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.2407
RefSeq:	YP_418365.1
UniProt:	Q2YLC8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTPDLPLTRL AAFVRQQTIA PEPLTGIGGT VSDVSRSSIA VRGLSRDARL GDTVAIRAGE GAPSLAEIIR VADLQVLVKP FDDRIMPSLG AAVFEEGPLR IRPAPEWRGR VINALGNAID GKGALKPGTR PMAAESLAPA ALRRARVDRG LRTGVNVIDI FTPLCFGQRI GIFAGSGVGK STLLAMMTRA ADFDTVVLAL TGERGREVRE MLEETMAGHL GKTITVVATG DESPMMRRLA PNTATAIAEY FRDLGQNVLL IVDSVTRFAH AAREVAIAAE EPPVARGYPP SVFSQLPRLL ERAGPGSAEA GGSITGIYSV LVDGDDHNDP VSDTIRGTLD GHIVLDRAIA AQGRFPAVDI PASVSRLAKH NWTPEQRKLV MQLRSMVARF EETRDLRAIG AYQKSHDGLL DQAVDFVPRI YEALQQSPET GLSDDPYNDL AAALRGDRQA QNA

NT Sequence

ATGACGCCTG ATCTGCCACT TACCCGCCTT GCTGCTTTTG TTCGCCAGCA AACAATAGCG CCAGAGCCGC TCACCGGTAT CGGGGGCACG GTGAGTGACG TTTCGCGTTC GTCTATTGCA GTGCGTGGCC TTTCGCGCGA TGCGCGGCTT GGCGATACGG TGGCAATCCG CGCTGGCGAG GGGGCGCCGA GCCTTGCCGA AATCATCCGT GTTGCCGATT TGCAGGTGCT GGTGAAACCG TTCGATGACC GGATCATGCC TTCGCTGGGG GCAGCGGTTT TCGAGGAAGG CCCGTTGCGC ATCCGCCCGG CGCCGGAATG GCGGGGCCGG GTCATCAATG CGCTTGGAAA TGCCATTGAC GGAAAGGGCG CGCTGAAACC GGGCACCCGC CCCATGGCGG CAGAAAGCCT TGCTCCGGCG GCTTTGCGCC GTGCGCGGGT GGATCGCGGC TTGCGTACCG GCGTTAATGT GATCGATATT TTCACGCCGC TCTGTTTTGG CCAGCGCATC GGCATTTTCG CAGGCTCCGG TGTCGGCAAA TCAACGCTTC TCGCCATGAT GACGCGCGCT GCCGATTTCG ATACGGTCGT GCTGGCGCTG ACGGGCGAGC GCGGGCGCGA AGTGCGCGAA ATGCTGGAAG AAACCATGGC CGGGCATTTG GGCAAGACCA TAACTGTGGT GGCGACCGGC GATGAAAGCC CGATGATGCG CCGCCTTGCG CCCAATACGG CGACGGCGAT TGCCGAATAT TTCCGCGATC TTGGCCAGAA TGTTCTGTTG ATCGTCGATT CCGTCACACG CTTTGCCCAT GCGGCACGCG AAGTGGCCAT TGCTGCCGAG GAGCCGCCGG TTGCACGCGG TTATCCGCCA AGTGTTTTCA GCCAGTTGCC CCGCCTTCTG GAGCGTGCCG GTCCCGGCAG TGCCGAAGCG GGTGGCAGCA TCACCGGTAT TTATTCCGTG CTGGTGGATG GTGACGATCA TAACGATCCG GTTTCCGATA CTATTCGCGG AACGCTGGAC GGCCACATTG TGCTCGACCG CGCCATTGCC GCGCAGGGGC GCTTTCCGGC GGTGGATATT CCGGCTTCCG TTTCGCGCCT TGCCAAACAC AACTGGACGC CGGAACAGCG TAAGCTGGTG ATGCAATTGC GCAGCATGGT TGCCCGGTTC GAGGAAACGC GCGACCTGCG CGCCATTGGC GCTTATCAGA AAAGCCATGA CGGGCTTCTG GACCAGGCGG TCGATTTTGT GCCGCGTATT TATGAAGCTT TACAGCAATC ACCGGAAACC GGCCTATCGG ACGATCCCTA TAACGACCTT GCCGCAGCAT TGCGCGGTGA CAGACAGGCG CAAAACGCA

Details for BrabA.17369.a.A1.GE31618

HARVESTED ON:	3/1/2011
SEQUENCED ON:	3/7/2011
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	77

Validated AA Sequence

MPSLGAAVFE EGPLRIRPAP EXRGRVINAL GNAIDGKGAL KPGTRXMAAE SLAPAALRRA RXDRGLRTGV NVIXIFTPLC FXQRIGIFAG SGVGKSTLLA MMTRAADFDT VVLALTGERG REVREMLEET MXGHLGKTIT VVATGDESPM MRRLAPNTAT AIAEYFRDLG QNVLLIVDSV TRFAHAAREV AIAAEEPPVA RGYPPSVFSQ LPRLLERAGP GSAEAGGSIT GIYSVLVDGD DHNDPVSDTI RGTLDGHIVL DRAIAAQGRF PAVDIPASVS RLAKHNWTPE QRKLVMQLRS MVARFEETRD LRAIGAYQKS HDGLLDQAVD FVPRIYEALQ QSPETGLSDD PYNDLAAALR GDRQAQ

Validated NT Sequence

tttgcgcctg tctgtcaccg cgcaatgctg cggcaaggtc gttataggga tcgtccgata ggccggtttc cggtgattgc tgtaaagctt cataaatacg cggcacaaaa tcgaccgcct ggtccagaag cccgtcatgg cttttctgat aagcgccaat ggcgcgcagg tcgcgcgttt cctcgaaccg ggcaaccatg ctgcgcaatt gcatcaccag cttacgctgt tccggcgtcc agttgtgttt ggcaaggcgc gaaacggaag ccggaatatc caccgccgga aagcgcccct gcgcggcaat ggcgcggtcg agcacaatgt ggccgtccag cgttccgcga atagtatcgg aaaccggatc gttatgatcg tcaccatcca ccagcacgga ataaataccg gtgatgctgc cacccgcttc ggcactgccg ggaccggcac gctccagaag gcggggcaac tggctgaaaa cacttggcgg ataaccgcgt gcaaccggcg gctcctcggc agcaatggcc acttcgcgtg ccgcatgggc aaagcgtgtg acggaatcga cgatcaacag aacattctgg ccaagatcgc ggaaatattc ggcaatcgcc gtcgccgtat tgggcgcaag gcggcgcatc atcgggcttt catcgccggt cgccaccaca gttatggtct tgcccaaatg cccggncatg gtttcttcca gcatttcgcg cacttcgcgc ccgcgctcgc ccgtcagcgc cagcacgacc gtatcgaaat cggcagcgcg cgtcatcatg gcgagaagcg ttgatttgcc gacaccggag cctgcgaaaa tgccgatgcg ctggncaaaa cagagcggcg tgaaaatntc gatcacatta acgccggtac gcaagccgcg atccncccgc gcacggcgca aagccgccgg agcaaggctt tctgccgcca tggngcgggt gcccggtttc agcgcgccct ttccgtcaat ggcatttcca agcgcattga tgacccggcc ccgncattcc ggcgccgggc ggatgcgcaa cgggccttcc tcgaaaaccg ctgcccccag cgaaggcatg atccggtcat cgaacggttt caccagcacc tgcaaatcgg caacacggat gatt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTPDLPLTR LAAFVRQQTI APEPLTGIGG TVSDVSRSSI AVRGLSRDAR LGDTVAIRAG EGAPSLAEII RVADLQVLVK PFDDRIMPSL GAAVFEEGPL RIRPAPEWRG RVINALGNAI DGKGALKPGT RPMAAESLAP AALRRARVDR GLRTGVNVID IFTPLCFGQR IGIFAGSGVG KSTLLAMMTR AADFDTVVLA LTGERGREVR EMLEETMAGH LGKTITVVAT GDESPMMRRL APNTATAIAE YFRDLGQNVL LIVDSVTRFA HAAREVAIAA EEPPVARGYP PSVFSQLPRL LERAGPGSAE AGGSITGIYS VLVDGDDHND PVSDTIRGTL DGHIVLDRAI AAQGRFPAVD IPASVSRLAK HNWTPEQRKL VMQLRSMVAR FEETRDLRAI GAYQKSHDGL LDQAVDFVPR IYEALQQSPE TGLSDDPYND LAAALRGDRQ AQNA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgcctgat ctgccactta cccgccttgc tgcttttgtt cgccagcaaa caatagcgcc agagccgctc accggtatcg ggggcacggt gagtgacgtt tcgcgttcgt ctattgcagt gcgtggcctt tcgcgcgatg cgcggcttgg cgatacggtg gcaatccgcg ctggcgaggg ggcgccgagc cttgccgaaa tcatccgtgt tgccgatttg caggtgctgg tgaaaccgtt cgatgaccgg atcatgcctt cgctgggggc agcggttttc gaggaaggcc cgttgcgcat ccgcccggcg ccggaatggc ggggccgggt catcaatgcg cttggaaatg ccattgacgg aaagggcgcg ctgaaaccgg gcacccgccc catggcggca gaaagccttg ctccggcggc tttgcgccgt gcgcgggtgg atcgcggctt gcgtaccggc gttaatgtga tcgatatttt cacgccgctc tgttttggcc agcgcatcgg cattttcgca ggctccggtg tcggcaaatc aacgcttctc gccatgatga cgcgcgctgc cgatttcgat acggtcgtgc tggcgctgac gggcgagcgc gggcgcgaag tgcgcgaaat gctggaagaa accatggccg ggcatttggg caagaccata actgtggtgg cgaccggcga tgaaagcccg atgatgcgcc gccttgcgcc caatacggcg acggcgattg ccgaatattt ccgcgatctt ggccagaatg ttctgttgat cgtcgattcc gtcacacgct ttgcccatgc ggcacgcgaa gtggccattg ctgccgagga gccgccggtt gcacgcggtt atccgccaag tgttttcagc cagttgcccc gccttctgga gcgtgccggt cccggcagtg ccgaagcggg tggcagcatc accggtattt attccgtgct ggtggatggt gacgatcata acgatccggt ttccgatact attcgcggaa cgctggacgg ccacattgtg ctcgaccgcg ccattgccgc gcaggggcgc tttccggcgg tggatattcc ggcttccgtt tcgcgccttg ccaaacacaa ctggacgccg gaacagcgta agctggtgat gcaattgcgc agcatggttg cccggttcga ggaaacgcgc gacctgcgcg ccattggcgc ttatcagaaa agccatgacg ggcttctgga ccaggcggtc gattttgtgc cgcgtattta tgaagcttta cagcaatcac cggaaaccgg cctatcggac gatccctata acgaccttgc cgcagcattg cgcggtgaca gacaggcgca aaacgcataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for BrabA.17369.a.A1.PS01097

PURIFICATION DATe:	5/18/2011
CONCENTRATION:	15.77mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	9
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	77

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGCCTGAT CTGCCACTTA CCCGCCTTGC TGCTTTTGTT CGCCAGCAAA CAATAGCGCC AGAGCCGCTC ACCGGTATCG GGGGCACGGT GAGTGACGTT TCGCGTTCGT CTATTGCAGT GCGTGGCCTT TCGCGCGATG CGCGGCTTGG CGATACGGTG GCAATCCGCG CTGGCGAGGG GGCGCCGAGC CTTGCCGAAA TCATCCGTGT TGCCGATTTG CAGGTGCTGG TGAAACCGTT CGATGACCGG ATCATGCCTT CGCTGGGGGC AGCGGTTTTC GAGGAAGGCC CGTTGCGCAT CCGCCCGGCG CCGGAATGGC GGGGCCGGGT CATCAATGCG CTTGGAAATG CCATTGACGG AAAGGGCGCG CTGAAACCGG GCACCCGCCC CATGGCGGCA GAAAGCCTTG CTCCGGCGGC TTTGCGCCGT GCGCGGGTGG ATCGCGGCTT GCGTACCGGC GTTAATGTGA TCGATATTTT CACGCCGCTC TGTTTTGGCC AGCGCATCGG CATTTTCGCA GGCTCCGGTG TCGGCAAATC AACGCTTCTC GCCATGATGA CGCGCGCTGC CGATTTCGAT ACGGTCGTGC TGGCGCTGAC GGGCGAGCGC GGGCGCGAAG TGCGCGAAAT GCTGGAAGAA ACCATGGCCG GGCATTTGGG CAAGACCATA ACTGTGGTGG CGACCGGCGA TGAAAGCCCG ATGATGCGCC GCCTTGCGCC CAATACGGCG ACGGCGATTG CCGAATATTT CCGCGATCTT GGCCAGAATG TTCTGTTGAT CGTCGATTCC GTCACACGCT TTGCCCATGC GGCACGCGAA GTGGCCATTG CTGCCGAGGA GCCGCCGGTT GCACGCGGTT ATCCGCCAAG TGTTTTCAGC CAGTTGCCCC GCCTTCTGGA GCGTGCCGGT CCCGGCAGTG CCGAAGCGGG TGGCAGCATC ACCGGTATTT ATTCCGTGCT GGTGGATGGT GACGATCATA ACGATCCGGT TTCCGATACT ATTCGCGGAA CGCTGGACGG CCACATTGTG CTCGACCGCG CCATTGCCGC GCAGGGGCGC TTTCCGGCGG TGGATATTCC GGCTTCCGTT TCGCGCCTTG CCAAACACAA CTGGACGCCG GAACAGCGTA AGCTGGTGAT GCAATTGCGC AGCATGGTTG CCCGGTTCGA GGAAACGCGC GACCTGCGCG CCATTGGCGC TTATCAGAAA AGCCATGACG GGCTTCTGGA CCAGGCGGTC GATTTTGTGC CGCGTATTTA TGAAGCTTTA CAGCAATCAC CGGAAACCGG CCTATCGGAC GATCCCTATA ACGACCTTGC CGCAGCATTG CGCGGTGACA GACAGGCGCA AAACGCATAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA