BrabA.17365.a

Glycosyl transferase, group 1

CENTER ID: BrabA.17365.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17365.a.A1.GE31614 Full length( BrabA.17365.a ) 1 372
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.579
RefSeq: YP_414007.1
UniProt: Q2YMN5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRIGVDARNL VNNITGISRY VMENCRELYE RGHELVLYTP EKPRVQLSSS EGFDFRVSNF QGPAGRLFWS QTVLPLQLRK DRIDVFWGPA HRLPALKGAI PSVVSIHDLV WYYASSTMRL QGWLADRFLM KGALRNADQI VAVSYATSSA ISSVFPQYTS KIQMVYPGVS HFNRIGTSNI LKEHRINKSY GLFIGTLEPR KNLIRLLEAY ANLPECVRNN FLLVVAGGKG WNLGDIGKTI NRLGIAEYTR LTGYVTDSDL NDLYRNARTL LMPSLYEGFG LPIIEAQAYG VPVITSNCSS MPEVAGDAAI LVDPLNVAEI SNALYRIATD NKLWQRKSLL ALQNIERFKW HNSIEKLESV FIKARESKLM TY
NT Sequence
ATGCGAATTG GTGTCGACGC TCGCAATTTA GTTAATAATA TAACTGGCAT ATCTAGGTAT GTCATGGAAA ATTGCCGAGA GCTTTATGAG CGAGGACATG AGCTTGTCTT GTATACACCA GAAAAGCCAC GGGTGCAGTT ATCCTCTTCA GAAGGATTCG ACTTTCGAGT TAGTAATTTT CAAGGGCCAG CGGGGCGGCT TTTTTGGTCT CAAACTGTTT TGCCACTACA GCTCAGAAAA GATCGAATTG ATGTTTTCTG GGGACCTGCC CACCGCCTCC CTGCGTTGAA GGGAGCGATT CCGAGCGTTG TGAGTATTCA CGACTTGGTT TGGTACTACG CATCGTCAAC AATGAGACTG CAAGGTTGGC TTGCAGATCG CTTCCTTATG AAAGGAGCGC TTAGGAATGC TGATCAAATT GTTGCTGTAT CATATGCAAC TTCATCAGCA ATTTCTTCGG TATTCCCTCA GTATACTTCA AAGATTCAAA TGGTATATCC GGGTGTCTCG CACTTCAATA GGATCGGTAC TTCGAACATC TTAAAAGAGC ATCGAATTAA CAAGTCGTAC GGCTTGTTCA TCGGTACCTT AGAGCCTAGA AAAAACCTTA TACGGCTCCT CGAAGCTTAT GCAAACTTAC CTGAATGTGT TCGAAATAAT TTTCTGCTGG TTGTAGCCGG TGGAAAGGGC TGGAACCTAG GAGATATTGG GAAGACTATA AATCGGCTTG GCATTGCTGA GTATACGCGT TTAACTGGGT ATGTTACAGA CAGCGATCTA AACGATTTAT ATAGAAATGC ACGGACGCTT CTCATGCCGT CTCTCTACGA AGGTTTTGGC TTACCCATAA TTGAGGCACA AGCCTACGGA GTTCCTGTGA TTACGTCAAA CTGTTCATCG ATGCCTGAGG TGGCTGGGGA TGCAGCAATC TTGGTCGATC CTCTGAACGT AGCCGAAATA TCGAATGCAC TTTATAGAAT CGCTACTGAT AATAAACTAT GGCAGCGGAA GTCATTACTT GCTTTACAAA ACATTGAGCG TTTCAAATGG CATAATAGCA TAGAAAAGCT GGAAAGTGTT TTCATCAAAG CCAGAGAATC TAAGCTCATG ACCTAT
Details for BrabA.17365.a.A1.GE31614
HARVESTED ON: 3/1/2011
SEQUENCED ON: 3/11/2011
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 76
PERCENT COVERAGE: 31
Validated AA Sequence
MSXXIXXSFM SEDMSLSXIH XXKPXXXLSS SEGFDFRVSN FQGPAGRLFW SQTVLPLQLX KDRIDVFWGP AHRLPALKGA IPSVVSIXDL VWYYASSTMR LQGXLADRFL MKGALRNAXX IXAXX
Validated NT Sequence
cnnnnnnnnn nnnnnnnntg cggnncagcc atgtttacgn ncactcctnt ngtnnatccn nnnnnnnnnn annannnnnn nncannnnnn nnnnnannca nnggncctgg nnccatgcna anngnngtcn acgctcgcaa tttanttnat aatataactg gcntatntag gnatgtcntg naanattgnc nanagcttta tgagcgagga catgagcttg tcttgnatac accannanaa gccangngng canttatcct cttcagaagg attcgacttt cgagttagta attttcaagg gccagcgggg cggctttttt ggtctcaaac tgttttgcca ctacagctca naaaagatcg aattgatgtt ttctggggac ctgcccaccg cctccctgcg ttgaagggag cgattccgag cgttgtgagt attcncgact tggtttggta ctacgcatcg tcaacaatga gactgcaagg nnggcttgca gatcgcttcc ttatgaaagg agcgcttagg aatgcnganc anattgntgc tgnnncatag caacttcatc agcaatttct tcggtattcc ctcagtatac ttcnnanatt nanatggnat atncgggngt nncgcacttc antaggatcg gnacttccaa catcttanaa nancatcnna annnnnnnnt cgtacggctn gntcntcgnn cctnagannc nnnnnaaaan nttnnannnn tcctnngaan ctnatgaana
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRIGVDARN LVNNITGISR YVMENCRELY ERGHELVLYT PEKPRVQLSS SEGFDFRVSN FQGPAGRLFW SQTVLPLQLR KDRIDVFWGP AHRLPALKGA IPSVVSIHDL VWYYASSTMR LQGWLADRFL MKGALRNADQ IVAVSYATSS AISSVFPQYT SKIQMVYPGV SHFNRIGTSN ILKEHRINKS YGLFIGTLEP RKNLIRLLEA YANLPECVRN NFLLVVAGGK GWNLGDIGKT INRLGIAEYT RLTGYVTDSD LNDLYRNART LLMPSLYEGF GLPIIEAQAY GVPVITSNCS SMPEVAGDAA ILVDPLNVAE ISNALYRIAT DNKLWQRKSL LALQNIERFK WHNSIEKLES VFIKARESKL MTY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgaattggt gtcgacgctc gcaatttagt taataatata actggcatat ctaggtatgt catggaaaat tgccgagagc tttatgagcg aggacatgag cttgtcttgt atacaccaga aaagccacgg gtgcagttat cctcttcaga aggattcgac tttcgagtta gtaattttca agggccagcg gggcggcttt tttggtctca aactgttttg ccactacagc tcagaaaaga tcgaattgat gttttctggg gacctgccca ccgcctccct gcgttgaagg gagcgattcc gagcgttgtg agtattcacg acttggtttg gtactacgca tcgtcaacaa tgagactgca aggttggctt gcagatcgct tccttatgaa aggagcgctt aggaatgctg atcaaattgt tgctgtatca tatgcaactt catcagcaat ttcttcggta ttccctcagt atacttcaaa gattcaaatg gtatatccgg gtgtctcgca cttcaatagg atcggtactt cgaacatctt aaaagagcat cgaattaaca agtcgtacgg cttgttcatc ggtaccttag agcctagaaa aaaccttata cggctcctcg aagcttatgc aaacttacct gaatgtgttc gaaataattt tctgctggtt gtagccggtg gaaagggctg gaacctagga gatattggga agactataaa tcggcttggc attgctgagt atacgcgttt aactgggtat gttacagaca gcgatctaaa cgatttatat agaaatgcac ggacgcttct catgccgtct ctctacgaag gttttggctt acccataatt gaggcacaag cctacggagt tcctgtgatt acgtcaaact gttcatcgat gcctgaggtg gctggggatg cagcaatctt ggtcgatcct ctgaacgtag ccgaaatatc gaatgcactt tatagaatcg ctactgataa taaactatgg cagcggaagt cattacttgc tttacaaaac attgagcgtt tcaaatggca taatagcata gaaaagctgg aaagtgtttt catcaaagcc agagaatcta agctcatgac ctattaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa