BrabA.17359.b

Glycosyl transferase, family 25

CENTER ID: BrabA.17359.b
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17359.b.A1.GE31608 Full length( BrabA.17359.b ) 1 260
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.1679
RefSeq: YP_414982.1
UniProt: Q2YRB5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKCYLINLDK SRDRLEFMAS QFERLGAQFE RVEAVNGRAM SPLELASFTQ ISKEWPAPLS PAEIGCFLSH RKCLEKIAAG EDAYAAVFED DIRLSQGSSR FLASDHWIPK QADIVKIDAY GHEVLISNPV KNEGPYSISR LRSRHLQTGG YVVSREAARK LLPLMEKVSA PVDHFLFDPN DGPFNDFEIY QISPAICRQS GMESTIGQNR RPKQRPSLLG LVWREAKRLV MRTRRNLKGF IANVTKTGRW GPIPFDRDIA
NT Sequence
ATGAAGTGTT ATCTTATTAA TCTCGACAAG AGCCGGGATC GCCTCGAATT CATGGCGTCT CAGTTTGAGC GCCTCGGCGC ACAATTCGAG CGTGTGGAAG CCGTAAATGG ACGAGCCATG TCGCCGCTTG AACTGGCTTC CTTTACTCAA ATAAGTAAAG AATGGCCCGC TCCTTTGTCG CCTGCCGAAA TTGGCTGTTT TCTTTCTCAT CGCAAATGCC TTGAAAAGAT TGCTGCCGGC GAAGATGCCT ATGCGGCAGT CTTTGAAGAT GACATTCGAT TGAGCCAGGG TTCCTCGCGG TTTTTGGCTT CAGATCACTG GATACCCAAG CAAGCGGATA TCGTCAAAAT CGACGCTTAC GGACATGAGG TATTGATTTC CAACCCTGTT AAAAATGAAG GCCCGTATTC GATTTCCCGG CTGCGCTCGC GACACTTGCA GACGGGCGGA TACGTTGTTT CGCGCGAAGC AGCTCGCAAG CTCCTTCCAT TGATGGAAAA AGTCTCAGCG CCGGTAGACC ATTTCTTGTT CGATCCCAAT GATGGGCCAT TCAACGATTT TGAAATTTAT CAGATTTCCC CTGCAATTTG CCGCCAGTCG GGAATGGAAA GCACAATCGG TCAGAATCGG CGCCCCAAAC AGCGCCCCTC TCTTTTGGGC TTGGTCTGGC GTGAAGCTAA AAGATTGGTT ATGCGAACCC GTCGCAATCT AAAAGGTTTC ATAGCCAACG TCACCAAAAC AGGCCGGTGG GGGCCCATTC CATTTGATCG GGATATTGCG
Details for BrabA.17359.b.A1.GE31608
HARVESTED ON: 3/1/2011
SEQUENCED ON: 3/7/2011
EXPECTED MW: 31kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 55
PERCENT COVERAGE: 87
Validated AA Sequence
MFXXLXXXXX XXTXXXXXXX KXVLXXXXXX XXXXTRXXXX XXXXXXXXXR XXXXXXXXXX XXXXXXGXXX LXXXTNGXXX XXXXXXXCFL SHRKCLEKIA AGEXAYAAXF EDDIRLSQGS SRFLASDHWX PKQADXVXID AYGHEVLISN PVXNEGPYSI SRLRSRHLQT GGYXVSREAA RKLLPLMXKV SAXXDHXLXX PXXGPFXXFE IYQISPAICR XSGMESTXXQ NRRPXXRPSL LXLVXA
Validated NT Sequence
gnngnncagc catgtttnnn nnactcnnnn nnnannnaca nnnnannaca annnnnnann annannnatn caaannggtc ctgnnncnnn gaanngnnat nnnannnnnn ncnacnagag nnnnnnntcn nnntnnngnn gnnnnnnnnn nnnngcntcg nnncnnnngn nngngcaann nnnnnnnnnn nnncnnnntn tnccnccnng gnnnnnnnnn nctnnnanca ntnacgaatg ggcnnnnnnt tngnngccng cnncnnnnnn nntgttttct ttcncatcgc aaatgccttg aaaagattgc tgccggcgaa natgcctatg cggcagnctt tgaagatgac attcgattga gccagggttc ctcgcggttt ttggcttcag atcactggan acccaagcaa gcggatntcg tcnaaatcga cgcttacgga catgaggtat tgatttccaa ccctgttaan aatgaaggcc cgtattcnat ttcccggctg cgctcgcgac acttgcagac nggcggatac nttgtttcgc gcgaagcagc tcgcaagctc cttccattga tgnnaaaagt ctcagcnncn gnngaccatt tnttgntcna tcccnatgan gggccattcn nnnnntttga aatttatcag atttcccctg caatttgccg ccngtcggga atggaaagca cannnngnca gaatcggcgc cccnnacanc gnccctctct tttggncttg gtntnggcgt gaagctnaaa natngnttat gcnnnnncgt nnnantctaa aacggntnnn anccnannnt nacnaaaana ngnnnngtgg gggcccnttc cnnttgatcn ggatnttgcg naataaannn nnnnnanaag ttntgcagcc ancttnnttn ngnnnnnnng ctgctannaa annncnnnng gaagnnnnnn nttnnctgct gnncnccncc tnanannnaa nnnnnnnnna nnaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKCYLINLD KSRDRLEFMA SQFERLGAQF ERVEAVNGRA MSPLELASFT QISKEWPAPL SPAEIGCFLS HRKCLEKIAA GEDAYAAVFE DDIRLSQGSS RFLASDHWIP KQADIVKIDA YGHEVLISNP VKNEGPYSIS RLRSRHLQTG GYVVSREAAR KLLPLMEKVS APVDHFLFDP NDGPFNDFEI YQISPAICRQ SGMESTIGQN RRPKQRPSLL GLVWREAKRL VMRTRRNLKG FIANVTKTGR WGPIPFDRDI A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagtgttat cttattaatc tcgacaagag ccgggatcgc ctcgaattca tggcgtctca gtttgagcgc ctcggcgcac aattcgagcg tgtggaagcc gtaaatggac gagccatgtc gccgcttgaa ctggcttcct ttactcaaat aagtaaagaa tggcccgctc ctttgtcgcc tgccgaaatt ggctgttttc tttctcatcg caaatgcctt gaaaagattg ctgccggcga agatgcctat gcggcagtct ttgaagatga cattcgattg agccagggtt cctcgcggtt tttggcttca gatcactgga tacccaagca agcggatatc gtcaaaatcg acgcttacgg acatgaggta ttgatttcca accctgttaa aaatgaaggc ccgtattcga tttcccggct gcgctcgcga cacttgcaga cgggcggata cgttgtttcg cgcgaagcag ctcgcaagct ccttccattg atggaaaaag tctcagcgcc ggtagaccat ttcttgttcg atcccaatga tgggccattc aacgattttg aaatttatca gatttcccct gcaatttgcc gccagtcggg aatggaaagc acaatcggtc agaatcggcg ccccaaacag cgcccctctc ttttgggctt ggtctggcgt gaagctaaaa gattggttat gcgaacccgt cgcaatctaa aaggtttcat agccaacgtc accaaaacag gccggtgggg gcccattcca tttgatcggg atattgcgta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a