BrabA.17353.a

Tryptophan synthase alpha chain

CENTER ID: BrabA.17353.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17353.a.A1.GE31600 Full length( BrabA.17353.a ) 1 279
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17353.a.A1.PS01092 Full length( BrabA.17353.a ) 1 279

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.2195
RefSeq: YP_415435.1
UniProt: Q2YQW7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTRIDTKFA ELKAEGRPAL VTYFMGGDPD LETALKVMKA LPKAGADVIE LGMPFSDPMA DGPAIQAAGL RALNAGQTLA KTLYMAAEFR KEDDTTPIVM MGYYNPIYVY GVERFLTDAK ASGVDGLIVV DLPSEMDAEL CIPAMKAGIN FIRLTTPTTD DKRLPKVLHN SSGFVYYVSM NGITGAAIAD TAKVGEAVRH IKKSTDLPIC VGFGVKTPEQ AAAIATHADS VVVGTAIVNA IAGELDEKGK VKGDPVAAAT RLVHALAESV RATRLEAAQ
NT Sequence
ATGACTACTC GCATCGACAC CAAATTCGCC GAACTGAAGG CCGAGGGCCG TCCGGCGCTC GTCACCTATT TCATGGGCGG CGATCCCGAT CTGGAAACCG CGCTGAAGGT GATGAAAGCC CTGCCGAAAG CCGGCGCTGA CGTGATCGAG CTTGGTATGC CCTTCTCCGA CCCGATGGCG GATGGACCCG CCATTCAGGC GGCTGGCCTT CGCGCCCTGA ACGCCGGACA GACGCTGGCG AAGACGCTTT ATATGGCAGC AGAATTCCGC AAGGAAGACG ATACGACCCC GATCGTCATG ATGGGCTATT ACAATCCGAT CTATGTCTAT GGCGTGGAGC GTTTTCTGAC CGATGCCAAG GCATCCGGCG TGGATGGGCT GATCGTTGTC GATCTTCCTT CCGAAATGGA TGCCGAGCTT TGCATTCCGG CAATGAAGGC GGGCATCAAT TTCATCCGCC TCACCACGCC GACCACGGAC GACAAGCGCC TGCCGAAAGT GCTGCATAAT TCATCGGGCT TTGTCTATTA TGTCTCGATG AACGGCATCA CCGGCGCGGC CATTGCCGAT ACGGCAAAGG TCGGCGAAGC GGTTCGCCAT ATCAAGAAAA GCACCGACCT GCCGATCTGT GTCGGTTTCG GCGTCAAGAC GCCGGAACAG GCTGCGGCAA TCGCAACCCA TGCCGATAGC GTCGTCGTGG GCACGGCCAT TGTCAACGCA ATTGCCGGTG AACTCGATGA AAAGGGCAAG GTGAAGGGTG ATCCGGTTGC CGCCGCAACC CGGCTTGTCC ACGCTCTTGC AGAGAGTGTG CGCGCCACAC GCCTTGAAGC CGCCCAA
Details for BrabA.17353.a.A1.GE31600
HARVESTED ON: 3/1/2011
SEQUENCED ON: 3/12/2015
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMTTRIDT KFAELKAEGR PALVTYFMGG DPDLETALKV MKALPKAGAD VIELGMPFSD PMADGPAIQA AGLRALNAGQ TLAKTLYMAA EFRKEDDTTP IVMMGYYNPI YVYGVERFLT DAKASGVDGL IVVDLPSEMD AELCIPAMKA GINFIRLTTP TTDDKRLPKV LHNSSGFVYY VSMNGITGAA IADTAKVGEA VRHIKKSTDL PICVGFGVKT PEQAAAIATH ADSVVVGTAI VNAIAGELDE KGKVKGDPVA AATRLVHALA ESVRATRLEA AQ
Validated NT Sequence
ttattattgg gcggcttcaa ggcgtgtggc gcgcacactc tctgcaagag cgtggacaag ccgggttgcg gcggcaaccg gatcaccctt caccttgccc ttttcatcga gttcaccggc aattgcgttg acaatggccg tgcccacgac gacgctatcg gcatgggttg cgattgccgc agcctgttcc ggcgtcttga cgccgaaacc gacacagatc ggcaggtcgg tgcttttctt gatatggcga accgcttcgc cgacctttgc cgtatcggca atggccgcgc cggtgatgcc gttcatcgag acataataga caaagcccga tgaattatgc agcactttcg gcaggcgctt gtcgtccgtg gtcggcgtgg tgaggcggat gaaattgatg cccgccttca ttgccggaat gcaaagctcg gcatccattt cggaaggaag atcgacaacg atcagcccat ccacgccgga tgccttggca tcggtcagaa aacgctccac gccatagaca tagatcggat tgtaatagcc catcatgacg atcggggtcg tatcgtcttc cttgcggaat tctgctgcca tataaagcgt cttcgccagc gtctgtccgg cgttcagggc gcgaaggcca gccgcctgaa tggcgggtcc atccgccatc gggtcggaga agggcatacc aagctcgatc acgtcagcgc cggctttcgg cagggctttc atcaccttca gcgcggtttc cagatcggga tcgccgccca tgaaataggt gacgagcgcc ggacggccct cggccttcag ttcggcgaat ttggtgtcga tgcgagtagt catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg ancc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTRIDTKF AELKAEGRPA LVTYFMGGDP DLETALKVMK ALPKAGADVI ELGMPFSDPM ADGPAIQAAG LRALNAGQTL AKTLYMAAEF RKEDDTTPIV MMGYYNPIYV YGVERFLTDA KASGVDGLIV VDLPSEMDAE LCIPAMKAGI NFIRLTTPTT DDKRLPKVLH NSSGFVYYVS MNGITGAAIA DTAKVGEAVR HIKKSTDLPI CVGFGVKTPE QAAAIATHAD SVVVGTAIVN AIAGELDEKG KVKGDPVAAA TRLVHALAES VRATRLEAAQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactactcgc atcgacacca aattcgccga actgaaggcc gagggccgtc cggcgctcgt cacctatttc atgggcggcg atcccgatct ggaaaccgcg ctgaaggtga tgaaagccct gccgaaagcc ggcgctgacg tgatcgagct tggtatgccc ttctccgacc cgatggcgga tggacccgcc attcaggcgg ctggccttcg cgccctgaac gccggacaga cgctggcgaa gacgctttat atggcagcag aattccgcaa ggaagacgat acgaccccga tcgtcatgat gggctattac aatccgatct atgtctatgg cgtggagcgt tttctgaccg atgccaaggc atccggcgtg gatgggctga tcgttgtcga tcttccttcc gaaatggatg ccgagctttg cattccggca atgaaggcgg gcatcaattt catccgcctc accacgccga ccacggacga caagcgcctg ccgaaagtgc tgcataattc atcgggcttt gtctattatg tctcgatgaa cggcatcacc ggcgcggcca ttgccgatac ggcaaaggtc ggcgaagcgg ttcgccatat caagaaaagc accgacctgc cgatctgtgt cggtttcggc gtcaagacgc cggaacaggc tgcggcaatc gcaacccatg ccgatagcgt cgtcgtgggc acggccattg tcaacgcaat tgccggtgaa ctcgatgaaa agggcaaggt gaagggtgat ccggttgccg ccgcaacccg gcttgtccac gctcttgcag agagtgtgcg cgccacacgc cttgaagccg cccaataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BrabA.17353.a.A1.PS01092
PURIFICATION DATe: 5/24/2011
CONCENTRATION: 38.56mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMTTRIDT KFAELKAEGR PALVTYFMGG DPDLETALKV MKALPKAGAD VIELGMPFSD PMADGPAIQA AGLRALNAGQ TLAKTLYMAA EFRKEDDTTP IVMMGYYNPI YVYGVERFLT DAKASGVDGL IVVDLPSEMD AELCIPAMKA GINFIRLTTP TTDDKRLPKV LHNSSGFVYY VSMNGITGAA IADTAKVGEA VRHIKKSTDL PICVGFGVKT PEQAAAIATH ADSVVVGTAI VNAIAGELDE KGKVKGDPVA AATRLVHALA ESVRATRLEA AQ
Validated NT Sequence
ttattattgg gcggcttcaa ggcgtgtggc gcgcacactc tctgcaagag cgtggacaag ccgggttgcg gcggcaaccg gatcaccctt caccttgccc ttttcatcga gttcaccggc aattgcgttg acaatggccg tgcccacgac gacgctatcg gcatgggttg cgattgccgc agcctgttcc ggcgtcttga cgccgaaacc gacacagatc ggcaggtcgg tgcttttctt gatatggcga accgcttcgc cgacctttgc cgtatcggca atggccgcgc cggtgatgcc gttcatcgag acataataga caaagcccga tgaattatgc agcactttcg gcaggcgctt gtcgtccgtg gtcggcgtgg tgaggcggat gaaattgatg cccgccttca ttgccggaat gcaaagctcg gcatccattt cggaaggaag atcgacaacg atcagcccat ccacgccgga tgccttggca tcggtcagaa aacgctccac gccatagaca tagatcggat tgtaatagcc catcatgacg atcggggtcg tatcgtcttc cttgcggaat tctgctgcca tataaagcgt cttcgccagc gtctgtccgg cgttcagggc gcgaaggcca gccgcctgaa tggcgggtcc atccgccatc gggtcggaga agggcatacc aagctcgatc acgtcagcgc cggctttcgg cagggctttc atcaccttca gcgcggtttc cagatcggga tcgccgccca tgaaataggt gacgagcgcc ggacggccct cggccttcag ttcggcgaat ttggtgtcga tgcgagtagt catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg ancc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTRIDTKF AELKAEGRPA LVTYFMGGDP DLETALKVMK ALPKAGADVI ELGMPFSDPM ADGPAIQAAG LRALNAGQTL AKTLYMAAEF RKEDDTTPIV MMGYYNPIYV YGVERFLTDA KASGVDGLIV VDLPSEMDAE LCIPAMKAGI NFIRLTTPTT DDKRLPKVLH NSSGFVYYVS MNGITGAAIA DTAKVGEAVR HIKKSTDLPI CVGFGVKTPE QAAAIATHAD SVVVGTAIVN AIAGELDEKG KVKGDPVAAA TRLVHALAES VRATRLEAAQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACTACTCGC ATCGACACCA AATTCGCCGA ACTGAAGGCC GAGGGCCGTC CGGCGCTCGT CACCTATTTC ATGGGCGGCG ATCCCGATCT GGAAACCGCG CTGAAGGTGA TGAAAGCCCT GCCGAAAGCC GGCGCTGACG TGATCGAGCT TGGTATGCCC TTCTCCGACC CGATGGCGGA TGGACCCGCC ATTCAGGCGG CTGGCCTTCG CGCCCTGAAC GCCGGACAGA CGCTGGCGAA GACGCTTTAT ATGGCAGCAG AATTCCGCAA GGAAGACGAT ACGACCCCGA TCGTCATGAT GGGCTATTAC AATCCGATCT ATGTCTATGG CGTGGAGCGT TTTCTGACCG ATGCCAAGGC ATCCGGCGTG GATGGGCTGA TCGTTGTCGA TCTTCCTTCC GAAATGGATG CCGAGCTTTG CATTCCGGCA ATGAAGGCGG GCATCAATTT CATCCGCCTC ACCACGCCGA CCACGGACGA CAAGCGCCTG CCGAAAGTGC TGCATAATTC ATCGGGCTTT GTCTATTATG TCTCGATGAA CGGCATCACC GGCGCGGCCA TTGCCGATAC GGCAAAGGTC GGCGAAGCGG TTCGCCATAT CAAGAAAAGC ACCGACCTGC CGATCTGTGT CGGTTTCGGC GTCAAGACGC CGGAACAGGC TGCGGCAATC GCAACCCATG CCGATAGCGT CGTCGTGGGC ACGGCCATTG TCAACGCAAT TGCCGGTGAA CTCGATGAAA AGGGCAAGGT GAAGGGTGAT CCGGTTGCCG CCGCAACCCG GCTTGTCCAC GCTCTTGCAG AGAGTGTGCG CGCCACACGC CTTGAAGCCG CCCAATAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA