SSGCID: Target Page

BrabA.17352.b

phosphinothricin N-acetyltransferase

CENTER ID:	BrabA.17352.b
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17352.b.B1.GE37726	Full length( BrabA.17352.b )		1	119

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel86222_0088

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPVIRDFQPA DIETITAIYT QAVLTGTGSY EIEPPTMDEM AKRFAAFADQ GFPILVAEAD GRVLGYAYAS YFRVRPAYRW LAEDSILSPP MRRGRALASF CCANSSRGFP PLGFASCWQ

NT Sequence

ATGCCCGTCA TTCGTGATTT CCAGCCTGCC GATATCGAAA CCATCACCGC CATCTATACG CAAGCCGTGC TGACCGGTAC CGGCTCCTAT GAGATCGAGC CGCCGACAAT GGACGAAATG GCAAAGCGTT TTGCGGCATT TGCGGACCAG GGCTTTCCGA TCCTCGTCGC GGAGGCGGAT GGCCGGGTTC TGGGCTATGC CTATGCGAGC TATTTCCGCG TCAGGCCAGC CTATCGCTGG CTTGCCGAGG ATTCGATCTT ATCGCCCCCG ATGCGAAGGG GCAGGGCATT GGCAAGCTTC TGCTGCGCGA ACTCATCGCG CGGATTTCCG CCCTTGGGTT TCGCCAGTTG CTGGCAG

Details for BrabA.17352.b.B1.GE37726

HARVESTED ON:	2/5/2014
SEQUENCED ON:	2/6/2014
EXPECTED MW:	14kDa
OBSERVED MW:	14kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMP VIRDFQPADI ETITAIYTQA VLTGTGSYEI EPPTMDEMAK RFAAFADQGF PILVAEADGR VLGYAYASYF RVRPAYRWLA EDSILSPPMR RGRALASFCC ANSSRGFPPL GFASCWQ

Validated NT Sequence

cnnnnnnnnt tccnctagaa tattttgttt aactttaaga aggagatata ccatggctca ccaccaccac caccatatgc ccgtcattcg tgatttccag cctgccgata tcgaaaccat caccgccatc tatacgcaag ccgtgctgac cggtaccggc tcctatgaga tcgagccgcc gacaatggac gaaatggcaa agcgttttgc ggcatttgcg gaccagggct ttccgatcct cgtcgcggag gcggatggcc gggttctggg ctatgcctat gcgagctatt tccgcgtcag gccagcctat cgctggcttg ccgaggattc gatcttatcg cccccgatgc gaaggggcag ggcattggca agcttctgct gcgcgaactc atcgcgcgga tttccgccct tgggtttcgc cagttgctgg cagtaagtga gtaagatagg atccggctgc taacnggnnn ntntannnnn ctgnnntcgc cgacgctcgg gttcttgcgc cagatcacgc tcttcctcgg ccacctcgta gtgaatatca tgcacgtcag ctctngccac ccccagggcg acacgatcgt ctggctgcca tcgcagaacg tgctgttatc atgctaccat gtcgaaatcg actccccgtg ctattgccgc gacacgcagc tcgaaaagtc gccggcctcg ctcaatgcgc tacgcacgct cagcgacgag aagcacgtgc cgtgccgcga cttcccgctg ctgagtccgg ctgaatcaac aaagtcctct actacgctaa tgatgtcgtc gctacncttc ttgcgcangg cacgcaatgc ntcataacgc gatctcgacc tgaacgcgca ttgcgctcac gctagaagcg tggacccgta tgtcggccac gtgatcatct acgancactg tctgccttcg acgtgcgntt gcgttngact aggcngtgga ctttttcatc tctgcatctt gacnngcang cgacaacgac atctgnncnc gctgcanact attgagtann agacnnncgc caantcaaga gnnatcngna cgaaggnnnn attntctnng aaatcttttc atagaagaaa gtctaccann ntcgtccgcc tgnctataan attnctattt tanaatanna nagccnntcn tac

Expected Protein Sequence

MAHHHHHHMP VIRDFQPADI ETITAIYTQA VLTGTGSYEI EPPTMDEMAK RFAAFADQGF PILVAEADGR VLGYAYASYF RVRPAYRWLA EDSILSPPMR RGRALASFCC ANSSRGFPPL GFASCWQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg tcattcgtga tttccagcct gccgatatcg aaaccatcac cgccatctat acgcaagccg tgctgaccgg taccggctcc tatgagatcg agccgccgac aatggacgaa atggcaaagc gttttgcggc atttgcggac cagggctttc cgatcctcgt cgcggaggcg gatggccggg ttctgggcta tgcctatgcg agctatttcc gcgtcaggcc agcctatcgc tggcttgccg aggattcgat cttatcgccc ccgatgcgaa ggggcagggc attggcaagc ttctgctgcg cgaactcatc gcgcggattt ccgcccttgg gtttcgccag ttgctggcag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat