BrabA.17351.a

Indole-3-glycerol phosphate synthase

CENTER ID: BrabA.17351.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: in PDB
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.17351.a.A1.GE31598 Full length( BrabA.17351.a ) 1 268
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.17351.a.A1.PS01096 Full length( BrabA.17351.a ) 1 268
Structures
3TSM
DEPOSITED: 9/13/2011
DETERMINATION: XRay
CLONE: BrabA.17351.a.A1.GE31598
PROTEIN: BrabA.17351.a.A1.PS01096
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_129980
PATRIC ID: fig|359391.4.peg.1211
RefSeq: YP_414555.1
UniProt: Q2YRR4
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTDILRKIE AYKREEIAAA KARLALDELK ARTRDQSAPR GFLKALEAKR AAGQFALIAE IKKASPSKGL IRPDFDPPAL AKAYEEGGAA CLSVLTDTPS FQGAPEFLTA ARQACSLPAL RKDFLFDPYQ VYEARSWGAD CILIIMASVD DDLAKELEDT AFALGMDALI EVHDEAEMER ALKLSSRLLG VNNRNLRSFE VNLAVSERLA KMAPSDRLLV GESGIFTHED CLRLEKSGIG TFLIGESLMR QHDVAAATRA LLTGAEKL
NT Sequence
ATGTCCACCG ATATTCTTCG CAAGATCGAA GCCTATAAGC GGGAAGAAAT AGCCGCCGCC AAGGCGCGTC TTGCCCTTGA TGAACTGAAG GCCCGGACGA GGGATCAGTC AGCGCCGCGC GGTTTTCTGA AGGCACTTGA AGCCAAGCGC GCCGCCGGTC AATTTGCACT GATCGCGGAG ATAAAGAAAG CAAGCCCGTC CAAAGGCTTG ATCCGCCCCG ATTTCGACCC GCCAGCGCTT GCAAAGGCTT ATGAAGAAGG CGGTGCGGCC TGTCTTTCCG TGCTGACCGA CACGCCTTCC TTTCAGGGCG CGCCGGAATT CCTCACGGCC GCACGCCAGG CTTGCAGCCT GCCCGCATTG CGCAAGGATT TCCTGTTCGA CCCCTATCAG GTCTATGAAG CGCGTAGCTG GGGAGCGGAT TGCATTCTCA TCATCATGGC CAGCGTCGAT GACGATCTTG CAAAAGAGCT GGAAGACACG GCTTTCGCAC TGGGCATGGA TGCGCTGATC GAAGTGCATG ACGAAGCTGA AATGGAACGC GCCCTGAAGC TTTCCTCGCG CCTGCTCGGC GTCAACAATC GCAATCTGCG CAGCTTCGAG GTCAATCTCG CCGTTTCCGA GCGGCTTGCG AAAATGGCTC CGTCCGACCG TCTGCTGGTT GGCGAAAGCG GCATCTTCAC GCATGAGGAC TGCCTGCGGC TTGAAAAGTC CGGCATCGGC ACTTTTCTGA TAGGCGAAAG CCTTATGCGA CAACATGACG TTGCGGCAGC CACCCGCGCG CTTTTGACGG GTGCGGAAAA GCTA
Details for BrabA.17351.a.A1.GE31598
HARVESTED ON: 3/1/2011
SEQUENCED ON: 3/7/2011
EXPECTED MW: 31kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSTDILRKI EAYKREEIAA AKARLALDEL KARTRDQSAP RGFLKALEAK RAAGQFALIA EIKKASPSKG LIRPDFDPPA LAKAYEEGGA ACLSVLTDTP SFQGAPEFLT AARQACSLPA LRKDFLFDPY QVYEARSWGA DCILIIMASV DDDLAKELED TAFALGMDAL IEVHDEAEME RALKLSSRLL GVNNRNLRSF EVNLAVSERL AKMAPSDRLL VGESGIFTHE DCLRLEKSGI GTFLIGESLM RQHDVAAATR ALLTGAE
Validated NT Sequence
tttccgcacc cgtcaaaagc gcgcgggtgg ctgccgcaac gtcatgttgt cgcataaggc tttcgcctat cagaaaagtg ccgatgccgg acttttcaag ccgcaggcag tcctcatgcg tgaagatgcc gctttcgcca accagcagac ggtcggacgg agccattttc gcaagccgct cggaaacggc gagattgacc tcgaagctgc gcagattgcg attgttgacg ccgagcaggc gcgaggaaag cttcagggcg cgttccattt cagcttcgtc atgcacttcg atcagcgcat ccatgcccag tgcgaaagcc gtgtcttcca gctcttttgc aagatcgtca tcgacgctgg ccatgatgat gagaatgcaa tccgctcccc agctacgcgc ttcatagacc tgataggggt cgaacaggaa atccttgcgc aatgcgggca ggctgcaagc ctggcgtgcg gccgtgagga attccggcgc gccctgaaag gaaggcgtgt cggtcagcac ggaaagacag gccgcaccgc cttcttcata agcctttgca agcgctggcg ggtcgaaatc ggggcggatc aagcctttgg acgggcttgc tttctttatc tccgcgatca gtgcaaattg accggcggcg cgcttggctt caagtgcctt cagaaaaccg cgcggcgctg actgatccct cgtccgggcc ttcagttcat caagggcaag acgcgccttg gcggcggcta tttcttcccg cttataggct tcgatcttgc gaagaatatc ggtggacatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc atgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTDILRKI EAYKREEIAA AKARLALDEL KARTRDQSAP RGFLKALEAK RAAGQFALIA EIKKASPSKG LIRPDFDPPA LAKAYEEGGA ACLSVLTDTP SFQGAPEFLT AARQACSLPA LRKDFLFDPY QVYEARSWGA DCILIIMASV DDDLAKELED TAFALGMDAL IEVHDEAEME RALKLSSRLL GVNNRNLRSF EVNLAVSERL AKMAPSDRLL VGESGIFTHE DCLRLEKSGI GTFLIGESLM RQHDVAAATR ALLTGAEKL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccaccgat attcttcgca agatcgaagc ctataagcgg gaagaaatag ccgccgccaa ggcgcgtctt gcccttgatg aactgaaggc ccggacgagg gatcagtcag cgccgcgcgg ttttctgaag gcacttgaag ccaagcgcgc cgccggtcaa tttgcactga tcgcggagat aaagaaagca agcccgtcca aaggcttgat ccgccccgat ttcgacccgc cagcgcttgc aaaggcttat gaagaaggcg gtgcggcctg tctttccgtg ctgaccgaca cgccttcctt tcagggcgcg ccggaattcc tcacggccgc acgccaggct tgcagcctgc ccgcattgcg caaggatttc ctgttcgacc cctatcaggt ctatgaagcg cgtagctggg gagcggattg cattctcatc atcatggcca gcgtcgatga cgatcttgca aaagagctgg aagacacggc tttcgcactg ggcatggatg cgctgatcga agtgcatgac gaagctgaaa tggaacgcgc cctgaagctt tcctcgcgcc tgctcggcgt caacaatcgc aatctgcgca gcttcgaggt caatctcgcc gtttccgagc ggcttgcgaa aatggctccg tccgaccgtc tgctggttgg cgaaagcggc atcttcacgc atgaggactg cctgcggctt gaaaagtccg gcatcggcac ttttctgata ggcgaaagcc ttatgcgaca acatgacgtt gcggcagcca cccgcgcgct tttgacgggt gcggaaaagc tataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BrabA.17351.a.A1.PS01096
PURIFICATION DATe: 5/16/2011
CONCENTRATION: 62.93mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSTDILRKI EAYKREEIAA AKARLALDEL KARTRDQSAP RGFLKALEAK RAAGQFALIA EIKKASPSKG LIRPDFDPPA LAKAYEEGGA ACLSVLTDTP SFQGAPEFLT AARQACSLPA LRKDFLFDPY QVYEARSWGA DCILIIMASV DDDLAKELED TAFALGMDAL IEVHDEAEME RALKLSSRLL GVNNRNLRSF EVNLAVSERL AKMAPSDRLL VGESGIFTHE DCLRLEKSGI GTFLIGESLM RQHDVAAATR ALLTGAE
Validated NT Sequence
tttccgcacc cgtcaaaagc gcgcgggtgg ctgccgcaac gtcatgttgt cgcataaggc tttcgcctat cagaaaagtg ccgatgccgg acttttcaag ccgcaggcag tcctcatgcg tgaagatgcc gctttcgcca accagcagac ggtcggacgg agccattttc gcaagccgct cggaaacggc gagattgacc tcgaagctgc gcagattgcg attgttgacg ccgagcaggc gcgaggaaag cttcagggcg cgttccattt cagcttcgtc atgcacttcg atcagcgcat ccatgcccag tgcgaaagcc gtgtcttcca gctcttttgc aagatcgtca tcgacgctgg ccatgatgat gagaatgcaa tccgctcccc agctacgcgc ttcatagacc tgataggggt cgaacaggaa atccttgcgc aatgcgggca ggctgcaagc ctggcgtgcg gccgtgagga attccggcgc gccctgaaag gaaggcgtgt cggtcagcac ggaaagacag gccgcaccgc cttcttcata agcctttgca agcgctggcg ggtcgaaatc ggggcggatc aagcctttgg acgggcttgc tttctttatc tccgcgatca gtgcaaattg accggcggcg cgcttggctt caagtgcctt cagaaaaccg cgcggcgctg actgatccct cgtccgggcc ttcagttcat caagggcaag acgcgccttg gcggcggcta tttcttcccg cttataggct tcgatcttgc gaagaatatc ggtggacatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc atgg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTDILRKI EAYKREEIAA AKARLALDEL KARTRDQSAP RGFLKALEAK RAAGQFALIA EIKKASPSKG LIRPDFDPPA LAKAYEEGGA ACLSVLTDTP SFQGAPEFLT AARQACSLPA LRKDFLFDPY QVYEARSWGA DCILIIMASV DDDLAKELED TAFALGMDAL IEVHDEAEME RALKLSSRLL GVNNRNLRSF EVNLAVSERL AKMAPSDRLL VGESGIFTHE DCLRLEKSGI GTFLIGESLM RQHDVAAATR ALLTGAEKL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCACCGAT ATTCTTCGCA AGATCGAAGC CTATAAGCGG GAAGAAATAG CCGCCGCCAA GGCGCGTCTT GCCCTTGATG AACTGAAGGC CCGGACGAGG GATCAGTCAG CGCCGCGCGG TTTTCTGAAG GCACTTGAAG CCAAGCGCGC CGCCGGTCAA TTTGCACTGA TCGCGGAGAT AAAGAAAGCA AGCCCGTCCA AAGGCTTGAT CCGCCCCGAT TTCGACCCGC CAGCGCTTGC AAAGGCTTAT GAAGAAGGCG GTGCGGCCTG TCTTTCCGTG CTGACCGACA CGCCTTCCTT TCAGGGCGCG CCGGAATTCC TCACGGCCGC ACGCCAGGCT TGCAGCCTGC CCGCATTGCG CAAGGATTTC CTGTTCGACC CCTATCAGGT CTATGAAGCG CGTAGCTGGG GAGCGGATTG CATTCTCATC ATCATGGCCA GCGTCGATGA CGATCTTGCA AAAGAGCTGG AAGACACGGC TTTCGCACTG GGCATGGATG CGCTGATCGA AGTGCATGAC GAAGCTGAAA TGGAACGCGC CCTGAAGCTT TCCTCGCGCC TGCTCGGCGT CAACAATCGC AATCTGCGCA GCTTCGAGGT CAATCTCGCC GTTTCCGAGC GGCTTGCGAA AATGGCTCCG TCCGACCGTC TGCTGGTTGG CGAAAGCGGC ATCTTCACGC ATGAGGACTG CCTGCGGCTT GAAAAGTCCG GCATCGGCAC TTTTCTGATA GGCGAAAGCC TTATGCGACA ACATGACGTT GCGGCAGCCA CCCGCGCGCT TTTGACGGGT GCGGAAAAGC TATAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA