BrabA.17333.a
DegT/DnrJ/EryC1/StrS family protein
| CENTER ID: | BrabA.17333.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.17333.a.B1.GE37725 | Full length( BrabA.17333.a ) | 1 | 374 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4QGR
DEPOSITED: 5/23/2014
DETERMINATION: XRay
CLONE: BrabA.17333.a.B1.GE37725
PROTEIN: BrabA.17333.a.B1.PS02033
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | PATRIC_Locus_Tag:VBIBruMel86222_1675 |
| BV-BRC: | fig|359391.4.peg.1675 |
| RefSeq: | YP_414978.1 |
| UniProt: | Q2YRB9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQFIDLGAQR ARIENRLNAA ISKVVAEGRY ILGPEVAEFE KKLGEYLGVE HVIACANGTD ALQMPLMTRG IGPGHAVFVP SFTFAATAEV VALVGAEPVF VDVDPDSYNM NVEQLEAAIA ATIKEGRLEP KAIIPVDLFG LAASYNRITA IAEREGLFII EDAAQSIGGK RDNVMCGAFG HVGATSFYPA KPLGCYGDGG AMFTNDAELA DTLRSVLFHG KGETQYDNVR IGINSRLDTI QAAVLLEKLA ILEDEMEARD RIARRYNEAL KDVVKVPELP AGNRSAWAQY SIESENRDGL KAQLQAEGIP SVIYYVKPLH LQTAYKHYSV APGGLPVSES LPSRILSLPM HPYLSEADQD KIIGVIRGFH GKKA
NT Sequence
ATGCAGTTCA TTGATCTTGG AGCGCAGCGC GCGCGTATCG AAAATCGTCT CAATGCCGCC ATTTCCAAGG TTGTTGCGGA AGGCCGTTAT ATTCTTGGGC CGGAAGTGGC TGAATTTGAA AAAAAGCTTG GCGAATATCT GGGTGTGGAG CATGTCATCG CCTGTGCCAA TGGCACTGAC GCCTTGCAGA TGCCCCTGAT GACACGCGGC ATCGGGCCGG GCCATGCGGT TTTTGTTCCG TCCTTCACCT TTGCAGCGAC CGCTGAGGTT GTTGCGCTCG TCGGTGCAGA GCCGGTATTC GTTGATGTCG ATCCAGATAG CTACAACATG AATGTCGAGC AGCTCGAAGC TGCCATTGCC GCCACCATCA AGGAAGGTCG TCTGGAGCCG AAGGCGATCA TTCCGGTCGA TCTTTTTGGC CTTGCCGCCT CTTACAATCG CATCACCGCC ATTGCCGAGC GCGAAGGCCT GTTCATCATC GAGGATGCGG CCCAGTCCAT CGGCGGCAAG CGCGACAATG TCATGTGCGG CGCTTTCGGC CATGTGGGCG CAACCAGCTT CTATCCCGCC AAGCCGCTTG GCTGCTACGG TGACGGTGGC GCCATGTTCA CCAATGATGC AGAATTGGCA GATACACTGC GCTCCGTGCT GTTCCACGGC AAGGGCGAGA CGCAGTATGA CAATGTCCGC ATCGGCATCA ATTCTCGCCT GGATACGATA CAGGCGGCTG TTCTTCTGGA AAAGCTTGCC ATTCTCGAAG ATGAGATGGA AGCCCGCGAC CGCATTGCAA GGCGCTATAA TGAGGCGCTG AAGGATGTGG TGAAGGTGCC GGAACTTCCA GCCGGCAACC GCTCGGCCTG GGCGCAATAT TCTATCGAAA GCGAGAACCG CGACGGCTTG AAGGCACAAC TTCAGGCAGA AGGTATTCCG TCCGTCATCT ATTATGTGAA GCCTCTGCAC TTGCAGACGG CCTATAAGCA CTATTCCGTT GCCCCCGGTG GCCTGCCGGT TTCGGAAAGC CTGCCATCAC GCATTCTGAG CCTGCCGATG CATCCTTACC TTTCGGAAGC TGACCAGGAC AAGATTATCG GCGTGATCCG TGGCTTCCAC GGCAAGAAGG CC
Details for BrabA.17333.a.B1.GE37725
| HARVESTED ON: | 2/5/2014 |
| SEQUENCED ON: | 2/6/2014 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 42kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
MQFIDLGAQR ARIENRLNAA ISKVVAEGRY ILGPEVAEFE KKLGEYLGVE HVIACANGTD ALQMPLMTRG IGPGHAVFVP SFTFAATAEV VALVGAEPVF VDVDPDSYNM NVEQLEAAIA ATIKEGRLEP KAIIPVDLFG LAASYNRITA IAEREGLFII EDAAQSIGGK RDNVMCGAFG HVGATSFYPA KPLGCYGDGG AMFTNDAELA DTLRSVLFHG KGETQYDNVR IGINSRLDTI QAAVLLEKLA ILEDEMEARD RIARRYNEAL KDVVKVPELP AGNRSAWAQY SIESENRDGL KAQLQAEGIP SVIYYVKPLH LQTAYKHYSV APGGLPVSES LPSRILSLPM HPYLSEADQD KIIGVIR
Validated NT Sequence
cacggatcac gccgataatc ttgtcctggt cagcttccga aaggtaagga tgcatcggca ggctcagaat gcgtgatggc aggctttccg aaaccggcag gccaccgggg gcaacggaat agtgcttata ggccgtctgc aagtgcagag gcttcacata atagatgacg gacggaatac cttctgcctg aagttgtgcc ttcaagccgt cgcggttctc gctttcgata gaatattgcg cccaggccga gcggttgccg gctggaagtt ccggcacctt caccacatcc ttcagcgcct cattatagcg ccttgcaatg cggtcgcggg cttccatctc atcttcgaga atggcaagct tttccagaag aacagccgcc tgtatcgtat ccaggcgaga attgatgccg atgcggacat tgtcatactg cgtctcgccc ttgccgtgga acagcacgga gcgcagtgta tctgccaatt ctgcatcatt ggtgaacatg gcgccaccgt caccgtagca gccaagcggc ttggcgggat agaagctggt tgcgcccaca tggccgaaag cgccgcacat gacattgtcg cgcttgccgc cgatggactg ggccgcatcc tcgatgatga acaggccttc gcgctcggca atggcggtga tgcgattgta agaggcggca aggccaaaaa gatcgaccgg aatgatcgcc ttcggctcca gacgaccttc cttgatggtg gcggcaatgg cagcttcgag ctgctcgaca ttcatgttgt agctatctgg atcgacatca acgaataccg gctctgcacc gacgagcgca acaacctcag cggtcgctgc aaaggtgaag gacggaacaa aaaccgcatg gcccggcccg atgccgcgtg tcatcagggg catctgcaag gcgtcagtgc cattggcaca ggcgatgaca tgctccacac ccagatattc gccaagcttt ttttcaaatt cagccacttc cggcccaaga atataacggc cttccgcaac aaccttggaa atggcagcat tgagacgatt ttcgatacgc gcgcgctgcg ctccaagatc aatgaactgc atatgg
Expected Protein Sequence
MAHHHHHHMQ FIDLGAQRAR IENRLNAAIS KVVAEGRYIL GPEVAEFEKK LGEYLGVEHV IACANGTDAL QMPLMTRGIG PGHAVFVPSF TFAATAEVVA LVGAEPVFVD VDPDSYNMNV EQLEAAIAAT IKEGRLEPKA IIPVDLFGLA ASYNRITAIA EREGLFIIED AAQSIGGKRD NVMCGAFGHV GATSFYPAKP LGCYGDGGAM FTNDAELADT LRSVLFHGKG ETQYDNVRIG INSRLDTIQA AVLLEKLAIL EDEMEARDRI ARRYNEALKD VVKVPELPAG NRSAWAQYSI ESENRDGLKA QLQAEGIPSV IYYVKPLHLQ TAYKHYSVAP GGLPVSESLP SRILSLPMHP YLSEADQDKI IGVIRGFHGK KA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagt tcattgatct tggagcgcag cgcgcgcgta tcgaaaatcg tctcaatgcc gccatttcca aggttgttgc ggaaggccgt tatattcttg ggccggaagt ggctgaattt gaaaaaaagc ttggcgaata tctgggtgtg gagcatgtca tcgcctgtgc caatggcact gacgccttgc agatgcccct gatgacacgc ggcatcgggc cgggccatgc ggtttttgtt ccgtccttca cctttgcagc gaccgctgag gttgttgcgc tcgtcggtgc agagccggta ttcgttgatg tcgatccaga tagctacaac atgaatgtcg agcagctcga agctgccatt gccgccacca tcaaggaagg tcgtctggag ccgaaggcga tcattccggt cgatcttttt ggccttgccg cctcttacaa tcgcatcacc gccattgccg agcgcgaagg cctgttcatc atcgaggatg cggcccagtc catcggcggc aagcgcgaca atgtcatgtg cggcgctttc ggccatgtgg gcgcaaccag cttctatccc gccaagccgc ttggctgcta cggtgacggt ggcgccatgt tcaccaatga tgcagaattg gcagatacac tgcgctccgt gctgttccac ggcaagggcg agacgcagta tgacaatgtc cgcatcggca tcaattctcg cctggatacg atacaggcgg ctgttcttct ggaaaagctt gccattctcg aagatgagat ggaagcccgc gaccgcattg caaggcgcta taatgaggcg ctgaaggatg tggtgaaggt gccggaactt ccagccggca accgctcggc ctgggcgcaa tattctatcg aaagcgagaa ccgcgacggc ttgaaggcac aacttcaggc agaaggtatt ccgtccgtca tctattatgt gaagcctctg cacttgcaga cggcctataa gcactattcc gttgcccccg gtggcctgcc ggtttcggaa agcctgccat cacgcattct gagcctgccg atgcatcctt acctttcgga agctgaccag gacaagatta tcggcgtgat ccgtggcttc cacggcaaga aggcctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat