BrabA.17327.a

ATP/GTP-binding site motif A (P-loop):Substrate-binding region of ABC-type glycine betaine transport system

CENTER ID: BrabA.17327.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17327.a.A2.GE31597 Mature protein( BrabA.17327.a ) 25 288
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17327.a.A2.PW31499 Mature protein( BrabA.17327.a ) 25 288

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.2798
RefSeq: YP_418692.1
UniProt: Q2YKZ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLGKLTRFGL AAVVAGTMTM GAAFAQDGKT VKIGWAPWSD AEFVTKLARK LIEDNLGQKV ELVQTDVAPL YQGVSRGDID AMMMAWLPET HADYYKRVEG KVENLGPLYE GAKLGWIVPD YIPESEVSSI EDLKKPEVRE KLKGEIQGID PGAGLTRLSQ EAIKKYGLDY KLNISSEAAM LTTVDRANRS EGWFVATSWS PHWMFGKYKL RYIADPKGAL GGAEHIDAVA RKGFKEDDPK VAALLAKMSI PINELEAAMF DAQETSYEKA VDKYIADHPD RIKEWLSQ
NT Sequence
ATGTTGGGCA AACTAACCAG ATTCGGTCTG GCGGCCGTTG TGGCGGGAAC GATGACCATG GGCGCCGCTT TTGCGCAGGA TGGCAAAACC GTCAAGATCG GCTGGGCGCC ATGGTCAGAT GCCGAATTTG TCACCAAACT CGCCAGGAAG CTGATCGAAG ATAATCTCGG CCAGAAGGTG GAGCTGGTGC AAACCGACGT AGCCCCCCTT TATCAGGGCG TGAGCCGTGG CGATATCGAT GCCATGATGA TGGCGTGGCT ACCGGAAACC CATGCGGATT ACTACAAGCG CGTCGAAGGC AAGGTGGAAA ATCTGGGTCC GCTTTATGAA GGCGCGAAGC TTGGCTGGAT CGTGCCGGAT TATATCCCGG AAAGTGAAGT CAGCTCCATC GAAGACCTCA AGAAGCCAGA GGTTCGCGAG AAGCTGAAGG GCGAGATTCA GGGGATCGAT CCGGGCGCGG GCCTCACCCG CCTGTCGCAG GAAGCGATCA AGAAATATGG GCTGGATTAC AAGCTCAATA TTTCGAGCGA GGCGGCAATG CTGACCACGG TTGACCGTGC CAACCGCTCT GAAGGCTGGT TTGTTGCCAC TTCGTGGAGC CCGCACTGGA TGTTCGGCAA GTACAAGCTG CGCTATATCG CCGATCCGAA AGGTGCGCTA GGCGGTGCGG AGCATATCGA TGCGGTGGCG CGCAAGGGCT TCAAGGAGGA CGATCCGAAG GTTGCAGCCT TGCTGGCGAA AATGAGCATT CCGATCAACG AGCTTGAGGC GGCGATGTTT GATGCGCAGG AAACGTCCTA TGAAAAGGCC GTCGACAAAT ATATCGCCGA CCATCCGGAC CGTATCAAGG AATGGCTTTC GCAA
Details for BrabA.17327.a.A2.GE31597
HARVESTED ON: 3/1/2011
SEQUENCED ON: 3/7/2011
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMAQDGKT VKIGWAPWSD AEFVTKLARK LIEDNLGQKV ELVQTDVAPL YQGVSRGDID AMMMAWLPET HADYYKRVEG KVENLGPLYE GAKLGWIVPD YIPESEVSSI EDLKKPEVRE KLKGEIQGID PGAGLTRLSQ EAIKKYGLDY KLNISSEAAM LTTVDRANRS EGWFVATSWS PHWMFGKYKL RYIADPKGAL GGAEHIDAVA RKGFKEDDPK VAALLAKMSI PINELEAAMF DAQETSYEKA VDKYIADHPD RIKEWLSQ
Validated NT Sequence
ttattattgc gaaagccatt ccttgatacg gtccggatgg tcggcgatat atttgtcgac ggccttttca taggacgttt cctgcgcatc aaacatcgcc gcctcaagct cgttgatcgg aatgctcatt ttcgccagca aggctgcaac cttcggatcg tcctccttga agcccttgcg cgccaccgca tcgatatgct ccgcaccgcc tagcgcacct ttcggatcgg cgatatagcg cagcttgtac ttgccgaaca tccagtgcgg gctccacgaa gtggcaacaa accagccttc agagcggttg gcacggtcaa ccgtggtcag cattgccgcc tcgctcgaaa tattgagctt gtaatccagc ccatatttct tgatcgcttc ctgcgacagg cgggtgaggc ccgcgcccgg atcgatcccc tgaatctcgc ccttcagctt ctcgcgaacc tctggcttct tgaggtcttc gatggagctg acttcacttt ccgggatata atccggcacg atccagccaa gcttcgcgcc ttcataaagc ggacccagat tttccacctt gccttcgacg cgcttgtagt aatccgcatg ggtttccggt agccacgcca tcatcatggc atcgatatcg ccacggctca cgccctgata aaggggggct acgtcggttt gcaccagctc caccttctgg ccgagattat cttcgatcag cttcctggcg agtttggtga caaattcggc atctgaccat ggcgcccagc cgatcttgac ggttttgcca tcctgcgcca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAQDGKTVK IGWAPWSDAE FVTKLARKLI EDNLGQKVEL VQTDVAPLYQ GVSRGDIDAM MMAWLPETHA DYYKRVEGKV ENLGPLYEGA KLGWIVPDYI PESEVSSIED LKKPEVREKL KGEIQGIDPG AGLTRLSQEA IKKYGLDYKL NISSEAAMLT TVDRANRSEG WFVATSWSPH WMFGKYKLRY IADPKGALGG AEHIDAVARK GFKEDDPKVA ALLAKMSIPI NELEAAMFDA QETSYEKAVD KYIADHPDRI KEWLSQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgcaggat ggcaaaaccg tcaagatcgg ctgggcgcca tggtcagatg ccgaatttgt caccaaactc gccaggaagc tgatcgaaga taatctcggc cagaaggtgg agctggtgca aaccgacgta gccccccttt atcagggcgt gagccgtggc gatatcgatg ccatgatgat ggcgtggcta ccggaaaccc atgcggatta ctacaagcgc gtcgaaggca aggtggaaaa tctgggtccg ctttatgaag gcgcgaagct tggctggatc gtgccggatt atatcccgga aagtgaagtc agctccatcg aagacctcaa gaagccagag gttcgcgaga agctgaaggg cgagattcag gggatcgatc cgggcgcggg cctcacccgc ctgtcgcagg aagcgatcaa gaaatatggg ctggattaca agctcaatat ttcgagcgag gcggcaatgc tgaccacggt tgaccgtgcc aaccgctctg aaggctggtt tgttgccact tcgtggagcc cgcactggat gttcggcaag tacaagctgc gctatatcgc cgatccgaaa ggtgcgctag gcggtgcgga gcatatcgat gcggtggcgc gcaagggctt caaggaggac gatccgaagg ttgcagcctt gctggcgaaa atgagcattc cgatcaacga gcttgaggcg gcgatgtttg atgcgcagga aacgtcctat gaaaaggccg tcgacaaata tatcgccgac catccggacc gtatcaagga atggctttcg caataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BrabA.17327.a.A2.PW31499
PURIFICATION DATe: 5/13/2011
CONCENTRATION: 26.42mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMAQDGKT VKIGWAPWSD AEFVTKLARK LIEDNLGQKV ELVQTDVAPL YQGVSRGDID AMMMAWLPET HADYYKRVEG KVENLGPLYE GAKLGWIVPD YIPESEVSSI EDLKKPEVRE KLKGEIQGID PGAGLTRLSQ EAIKKYGLDY KLNISSEAAM LTTVDRANRS EGWFVATSWS PHWMFGKYKL RYIADPKGAL GGAEHIDAVA RKGFKEDDPK VAALLAKMSI PINELEAAMF DAQETSYEKA VDKYIADHPD RIKEWLSQ
Validated NT Sequence
ttattattgc gaaagccatt ccttgatacg gtccggatgg tcggcgatat atttgtcgac ggccttttca taggacgttt cctgcgcatc aaacatcgcc gcctcaagct cgttgatcgg aatgctcatt ttcgccagca aggctgcaac cttcggatcg tcctccttga agcccttgcg cgccaccgca tcgatatgct ccgcaccgcc tagcgcacct ttcggatcgg cgatatagcg cagcttgtac ttgccgaaca tccagtgcgg gctccacgaa gtggcaacaa accagccttc agagcggttg gcacggtcaa ccgtggtcag cattgccgcc tcgctcgaaa tattgagctt gtaatccagc ccatatttct tgatcgcttc ctgcgacagg cgggtgaggc ccgcgcccgg atcgatcccc tgaatctcgc ccttcagctt ctcgcgaacc tctggcttct tgaggtcttc gatggagctg acttcacttt ccgggatata atccggcacg atccagccaa gcttcgcgcc ttcataaagc ggacccagat tttccacctt gccttcgacg cgcttgtagt aatccgcatg ggtttccggt agccacgcca tcatcatggc atcgatatcg ccacggctca cgccctgata aaggggggct acgtcggttt gcaccagctc caccttctgg ccgagattat cttcgatcag cttcctggcg agtttggtga caaattcggc atctgaccat ggcgcccagc cgatcttgac ggttttgcca tcctgcgcca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAQDGKTVK IGWAPWSDAE FVTKLARKLI EDNLGQKVEL VQTDVAPLYQ GVSRGDIDAM MMAWLPETHA DYYKRVEGKV ENLGPLYEGA KLGWIVPDYI PESEVSSIED LKKPEVREKL KGEIQGIDPG AGLTRLSQEA IKKYGLDYKL NISSEAAMLT TVDRANRSEG WFVATSWSPH WMFGKYKLRY IADPKGALGG AEHIDAVARK GFKEDDPKVA ALLAKMSIPI NELEAAMFDA QETSYEKAVD KYIADHPDRI KEWLSQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGCAGGAT GGCAAAACCG TCAAGATCGG CTGGGCGCCA TGGTCAGATG CCGAATTTGT CACCAAACTC GCCAGGAAGC TGATCGAAGA TAATCTCGGC CAGAAGGTGG AGCTGGTGCA AACCGACGTA GCCCCCCTTT ATCAGGGCGT GAGCCGTGGC GATATCGATG CCATGATGAT GGCGTGGCTA CCGGAAACCC ATGCGGATTA CTACAAGCGC GTCGAAGGCA AGGTGGAAAA TCTGGGTCCG CTTTATGAAG GCGCGAAGCT TGGCTGGATC GTGCCGGATT ATATCCCGGA AAGTGAAGTC AGCTCCATCG AAGACCTCAA GAAGCCAGAG GTTCGCGAGA AGCTGAAGGG CGAGATTCAG GGGATCGATC CGGGCGCGGG CCTCACCCGC CTGTCGCAGG AAGCGATCAA GAAATATGGG CTGGATTACA AGCTCAATAT TTCGAGCGAG GCGGCAATGC TGACCACGGT TGACCGTGCC AACCGCTCTG AAGGCTGGTT TGTTGCCACT TCGTGGAGCC CGCACTGGAT GTTCGGCAAG TACAAGCTGC GCTATATCGC CGATCCGAAA GGTGCGCTAG GCGGTGCGGA GCATATCGAT GCGGTGGCGC GCAAGGGCTT CAAGGAGGAC GATCCGAAGG TTGCAGCCTT GCTGGCGAAA ATGAGCATTC CGATCAACGA GCTTGAGGCG GCGATGTTTG ATGCGCAGGA AACGTCCTAT GAAAAGGCCG TCGACAAATA TATCGCCGAC CATCCGGACC GTATCAAGGA ATGGCTTTCG CAATAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA