SSGCID: Target Page

BrabA.17320.a

ATP/GTP-binding site motif A (P-loop):AFG1-like ATPase

CENTER ID:	BrabA.17320.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17320.a.A1.GE31594	Full length( BrabA.17320.a )		1	387

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17320.a.A1.PS01086	Full length( BrabA.17320.a )		1	387

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.2008
RefSeq:	YP_415268.1
UniProt:	Q2YLR7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSFDGNLKAF PSVRERYEAM VAAGDVETDP AQLELTSRYD RLIGEICSKR LSRKSSALGW LFGKRRETAA TIKGLYVHGE VGRGKTMLMD MFFQLLPVER KRRAHFNDFM ADVHERIYAH RQAHKRGETK QDDPIPPVAE ALSQQAWVLC FDEFAVTDIA DAMILSRLFS ALFSRGVVLV ATSNVAPDNL YRDGLNRQLF LPFIDILKSH VDVINLDSRT DYRLEKLDRQ PVYLSPLGPE TERRMDAAWA AHKNGAEEKP DVIHIKGRDI EVPRAAAGAA RFSFDDLCAR PLGASDYIAI ANRYPTLFID NVPVLDYSRR NEAKRFILLI DVLYDHHARL FVSAEAQPEK LYIANSGTEA FEFDRTASRL FEMQSAEYLD GIPEGVA

NT Sequence

ATGTCTTTTG ATGGCAATCT GAAAGCCTTC CCTTCCGTCC GCGAACGTTA TGAAGCCATG GTTGCAGCGG GCGATGTCGA AACCGACCCC GCCCAGCTTG AACTTACCAG CCGATATGAC AGGCTGATCG GGGAGATTTG CTCAAAACGC CTTTCGCGCA AGTCGAGCGC GCTCGGCTGG CTGTTCGGCA AGCGCAGGGA AACGGCCGCG ACCATCAAGG GGCTTTATGT CCACGGTGAG GTGGGGCGCG GCAAGACCAT GCTGATGGAC ATGTTCTTCC AGCTTTTGCC GGTGGAGCGC AAGCGGCGCG CGCATTTCAA CGATTTCATG GCCGATGTGC ATGAGCGCAT TTATGCGCAT CGTCAGGCGC ACAAGCGCGG TGAAACCAAA CAGGATGATC CAATCCCGCC GGTGGCGGAG GCGCTGAGCC AGCAGGCATG GGTTCTGTGT TTCGATGAAT TTGCCGTGAC CGACATTGCG GACGCGATGA TCCTGTCGCG CCTGTTCAGT GCGCTGTTCT CGCGCGGTGT GGTGCTGGTC GCGACCTCGA ACGTCGCGCC GGACAATCTC TACCGCGACG GGTTGAACCG TCAGCTTTTC CTGCCTTTCA TCGACATTCT CAAGAGCCAT GTCGATGTCA TCAATCTCGA TTCGCGCACG GATTACCGTC TGGAAAAGCT TGACCGCCAG CCGGTCTATC TTTCCCCGCT GGGGCCTGAA ACCGAGCGCC GCATGGATGC GGCCTGGGCG GCACACAAGA ACGGTGCGGA AGAAAAGCCT GACGTGATCC ATATCAAGGG GCGTGATATC GAAGTGCCGC GCGCTGCTGC TGGCGCTGCA CGGTTCTCGT TCGATGATCT TTGTGCCAGG CCGCTTGGTG CATCCGACTA TATAGCGATC GCCAACCGCT ATCCGACGCT GTTTATCGAT AATGTGCCGG TACTGGATTA TTCGCGCCGC AACGAGGCAA AGCGGTTTAT TCTGCTGATC GACGTGCTTT ACGATCATCA TGCGCGGCTG TTCGTTTCGG CTGAGGCGCA ACCGGAGAAG CTTTATATCG CCAATAGCGG CACGGAGGCT TTTGAGTTCG ACCGCACGGC TTCACGTCTT TTCGAGATGC AGAGCGCGGA ATATCTGGAT GGGATACCCG AAGGCGTAGC C

Details for BrabA.17320.a.A1.GE31594

HARVESTED ON:	3/1/2011
SEQUENCED ON:	3/7/2011
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	94

Validated AA Sequence

MSFDGNLKAF PSVRERYEAM VAAGDVETDP AQLELTSRYD RLIGEICSKR LSRKSSALGW LFGKRRETAA TIKGLYVHGE VGRGKTMLMD MFFQLLPVER KRRAHFNDFM ADVHERIYAH RQAHKRGETK QDDPIPPVAE ALSQQAWVLC FDEFAVTDIA DAMILSRLFS ALFSRGVVLV ATSNVAPDNL YRDGLNRQLF LPFIDILKSH VDVINLDSRT DYRLEKLDRQ PVYLSPLGPE TERRMDAAWA AHKNGAEEKP DVIHIKGRDI EVPRAAAGAA RFSFDDLCAR PLGASDYIAI ANRYPTLFIX XXPXXDYSRR NEAKRFILLI DVLYDHHARL FVSXEAQPEK LYIAXSGTEA FEFDRTASRL FEMQSAEYXX GIPEGV

Validated NT Sequence

gntacgcctt cgggtatccc ntnnngatat tccgcgctct gcatctcgaa aagacgtgaa gccgtgcggt cgaactcaaa agcctccgtg ccgctnttgg cgatataaag cttctccggt tgcgcctcan ccgaaacgaa cagccgcgca tgatgatcgt aaagcacgtc gatcagcaga ataaaccgct ttgcctcgtt gcggcgcgaa taatccantn ncggcannnn nncgataaac agcgtcggat agcggttggc gatcgctata tagtcggatg caccaagcgg cctggcacaa agatcatcga acgagaaccg tgcagcgcca gcagcagcgc gcggcacttc gatatcacgc cccttgatat ggatcacgtc aggcttttct tccgcaccgt tcttgtgtgc cgcccaggcc gcatccatgc ggcgctcggt ttcaggcccc agcggggaaa gatagaccgg ctggcggtca agcttttcca gacggtaatc cgtgcgcgaa tcgagattga tgacatcgac atggctcttg agaatgtcga tgaaaggcag gaaaagctga cggttcaacc cgtcgcggta gagattgtcc ggcgcgacgt tcgaggtcgc gaccagcacc acaccgcgcg agaacagcgc actgaacagg cgcgacagga tcatcgcgtc cgcaatgtcg gtcacggcaa attcatcgaa acacagaacc catgcctgct ggctcagcgc ctccgccacc ggcgggattg gatcatcctg tttggtttca ccgcgcttgt gcgcctgacg atgcgcataa atgcgctcat gcacatcggc catgaaatcg ttgaaatgcg cgcgccgctt gcgctccacc ggcaaaagct ggaagaacat gtccatcagc atggtcttgc cgcgccccac ctcaccgtgg acataaagcc ccttgatggt cgcggccgtt tccctgcgct tgccgaacag ccagccgagc gcgctcgact tgcgcgaaag gcgttttgag caaatctccc cgatcagcct gtcatatcgg ctggtaagtt caagctgggc ggggtcggtt tcgacatcgc ccgctgcaac catggcttca taacgttcgc ggacggaagg gaaggctttc agattgccat caaaagacat cgaaccagga ccctgggtnt gagcttncan gg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSFDGNLKA FPSVRERYEA MVAAGDVETD PAQLELTSRY DRLIGEICSK RLSRKSSALG WLFGKRRETA ATIKGLYVHG EVGRGKTMLM DMFFQLLPVE RKRRAHFNDF MADVHERIYA HRQAHKRGET KQDDPIPPVA EALSQQAWVL CFDEFAVTDI ADAMILSRLF SALFSRGVVL VATSNVAPDN LYRDGLNRQL FLPFIDILKS HVDVINLDSR TDYRLEKLDR QPVYLSPLGP ETERRMDAAW AAHKNGAEEK PDVIHIKGRD IEVPRAAAGA ARFSFDDLCA RPLGASDYIA IANRYPTLFI DNVPVLDYSR RNEAKRFILL IDVLYDHHAR LFVSAEAQPE KLYIANSGTE AFEFDRTASR LFEMQSAEYL DGIPEGVA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcttttgat ggcaatctga aagccttccc ttccgtccgc gaacgttatg aagccatggt tgcagcgggc gatgtcgaaa ccgaccccgc ccagcttgaa cttaccagcc gatatgacag gctgatcggg gagatttgct caaaacgcct ttcgcgcaag tcgagcgcgc tcggctggct gttcggcaag cgcagggaaa cggccgcgac catcaagggg ctttatgtcc acggtgaggt ggggcgcggc aagaccatgc tgatggacat gttcttccag cttttgccgg tggagcgcaa gcggcgcgcg catttcaacg atttcatggc cgatgtgcat gagcgcattt atgcgcatcg tcaggcgcac aagcgcggtg aaaccaaaca ggatgatcca atcccgccgg tggcggaggc gctgagccag caggcatggg ttctgtgttt cgatgaattt gccgtgaccg acattgcgga cgcgatgatc ctgtcgcgcc tgttcagtgc gctgttctcg cgcggtgtgg tgctggtcgc gacctcgaac gtcgcgccgg acaatctcta ccgcgacggg ttgaaccgtc agcttttcct gcctttcatc gacattctca agagccatgt cgatgtcatc aatctcgatt cgcgcacgga ttaccgtctg gaaaagcttg accgccagcc ggtctatctt tccccgctgg ggcctgaaac cgagcgccgc atggatgcgg cctgggcggc acacaagaac ggtgcggaag aaaagcctga cgtgatccat atcaaggggc gtgatatcga agtgccgcgc gctgctgctg gcgctgcacg gttctcgttc gatgatcttt gtgccaggcc gcttggtgca tccgactata tagcgatcgc caaccgctat ccgacgctgt ttatcgataa tgtgccggta ctggattatt cgcgccgcaa cgaggcaaag cggtttattc tgctgatcga cgtgctttac gatcatcatg cgcggctgtt cgtttcggct gaggcgcaac cggagaagct ttatatcgcc aatagcggca cggaggcttt tgagttcgac cgcacggctt cacgtctttt cgagatgcag agcgcggaat atctggatgg gatacccgaa ggcgtagcct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for BrabA.17320.a.A1.PS01086

PURIFICATION DATe:	5/17/2011
CONCENTRATION:	60mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	150µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

MSFDGNLKAF PSVRERYEAM VAAGDVETDP AQLELTSRYD RLIGEICSKR LSRKSSALGW LFGKRRETAA TIKGLYVHGE VGRGKTMLMD MFFQLLPVER KRRAHFNDFM ADVHERIYAH RQAHKRGETK QDDPIPPVAE ALSQQAWVLC FDEFAVTDIA DAMILSRLFS ALFSRGVVLV ATSNVAPDNL YRDGLNRQLF LPFIDILKSH VDVINLDSRT DYRLEKLDRQ PVYLSPLGPE TERRMDAAWA AHKNGAEEKP DVIHIKGRDI EVPRAAAGAA RFSFDDLCAR PLGASDYIAI ANRYPTLFIX XXPXXDYSRR NEAKRFILLI DVLYDHHARL FVSXEAQPEK LYIAXSGTEA FEFDRTASRL FEMQSAEYXX GIPEGV

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTTTTGAT GGCAATCTGA AAGCCTTCCC TTCCGTCCGC GAACGTTATG AAGCCATGGT TGCAGCGGGC GATGTCGAAA CCGACCCCGC CCAGCTTGAA CTTACCAGCC GATATGACAG GCTGATCGGG GAGATTTGCT CAAAACGCCT TTCGCGCAAG TCGAGCGCGC TCGGCTGGCT GTTCGGCAAG CGCAGGGAAA CGGCCGCGAC CATCAAGGGG CTTTATGTCC ACGGTGAGGT GGGGCGCGGC AAGACCATGC TGATGGACAT GTTCTTCCAG CTTTTGCCGG TGGAGCGCAA GCGGCGCGCG CATTTCAACG ATTTCATGGC CGATGTGCAT GAGCGCATTT ATGCGCATCG TCAGGCGCAC AAGCGCGGTG AAACCAAACA GGATGATCCA ATCCCGCCGG TGGCGGAGGC GCTGAGCCAG CAGGCATGGG TTCTGTGTTT CGATGAATTT GCCGTGACCG ACATTGCGGA CGCGATGATC CTGTCGCGCC TGTTCAGTGC GCTGTTCTCG CGCGGTGTGG TGCTGGTCGC GACCTCGAAC GTCGCGCCGG ACAATCTCTA CCGCGACGGG TTGAACCGTC AGCTTTTCCT GCCTTTCATC GACATTCTCA AGAGCCATGT CGATGTCATC AATCTCGATT CGCGCACGGA TTACCGTCTG GAAAAGCTTG ACCGCCAGCC GGTCTATCTT TCCCCGCTGG GGCCTGAAAC CGAGCGCCGC ATGGATGCGG CCTGGGCGGC ACACAAGAAC GGTGCGGAAG AAAAGCCTGA CGTGATCCAT ATCAAGGGGC GTGATATCGA AGTGCCGCGC GCTGCTGCTG GCGCTGCACG GTTCTCGTTC GATGATCTTT GTGCCAGGCC GCTTGGTGCA TCCGACTATA TAGCGATCGC CAACCGCTAT CCGACGCTGT TTATCGATAA TGTGCCGGTA CTGGATTATT CGCGCCGCAA CGAGGCAAAG CGGTTTATTC TGCTGATCGA CGTGCTTTAC GATCATCATG CGCGGCTGTT CGTTTCGGCT GAGGCGCAAC CGGAGAAGCT TTATATCGCC AATAGCGGCA CGGAGGCTTT TGAGTTCGAC CGCACGGCTT CACGTCTTTT CGAGATGCAG AGCGCGGAAT ATCTGGATGG GATACCCGAA GGCGTAGCCT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA