BrabA.17315.a

Ribosomal-protein-alanine N-acetyltransferase

CENTER ID: BrabA.17315.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17315.a.A1.GE31589 Full length( BrabA.17315.a ) 1 216
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17315.a.A1.PW31491 Full length( BrabA.17315.a ) 1 216

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.1927
RefSeq: YP_415200.1
UniProt: Q2YLL9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVVEEESYEA SCCKREADYA DASPGLEKEL GYVLDCPILV TERLVLRPPH AEDVDAISYL ANNARVSSML ARMPHPYTRE NALDFVGRVR KGEMGNCIYA ITQAETGIFM GCCGIHPYKH GEGLEIGYWL GEPYWGHGFA TEAAHALIDL AFRATGIGRL HVSCRASNGG SRRVIHKCGF QFSGMGMADS LAAGNVPVEH YVLDRRTWIG LRSWQS
NT Sequence
ATGGTTGTCG AAGAAGAAAG CTACGAAGCA AGTTGTTGTA AGCGGGAAGC GGATTATGCC GATGCATCTC CCGGCTTGGA AAAGGAGCTG GGCTATGTGC TGGATTGCCC CATTCTCGTC ACGGAAAGGC TGGTTCTGCG CCCGCCCCAT GCCGAAGACG TGGATGCCAT TTCCTATCTT GCCAACAATG CTCGCGTATC CAGCATGCTT GCCCGCATGC CGCATCCATA TACGCGAGAA AACGCCCTCG ATTTTGTGGG GCGCGTGCGA AAAGGCGAGA TGGGCAATTG CATCTATGCG ATTACGCAAG CAGAGACCGG CATTTTCATG GGGTGCTGCG GCATCCATCC GTACAAACAT GGCGAAGGGC TGGAAATCGG TTACTGGCTG GGGGAACCCT ATTGGGGCCA CGGCTTTGCG ACCGAGGCCG CTCATGCCCT GATTGATCTG GCCTTTCGCG CGACGGGCAT CGGGCGGCTG CATGTTTCAT GCCGGGCCAG CAATGGCGGC TCGCGCCGGG TGATCCACAA GTGCGGCTTT CAGTTCAGCG GCATGGGCAT GGCGGATTCG CTTGCCGCAG GAAATGTGCC GGTCGAGCAC TATGTTCTGG ACAGGCGTAC ATGGATCGGT CTGAGAAGCT GGCAATCA
Details for BrabA.17315.a.A1.GE31589
HARVESTED ON: 3/1/2011
SEQUENCED ON: 3/7/2011
EXPECTED MW: 26kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 51
PERCENT COVERAGE: 71
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVVEEESYE ASCCKREADY ADASPGLEKE LGYVLDCPHS RHGKAGSAPA PCRRRGCHFL SCQQCSRIQH ACPHAASIYA RKRPRFCGAR AKRRDGQLHL CDYASRDRHF HGVLRHPSVQ TWRRAGNRLL AGGTLLGPRL CDRGRSCPD
Validated NT Sequence
ccagcttctc agaccgatcc atgtacgcct gtccagaaca tagtgctcga ccggcacatt tcntgcggca agcgaatccg ccatgcccat gccgctgaac tgaaagccgc acttgtggat cacccggcgc gagccgccat tgctggcccg gcatgaaaca tgcagccgcc cgatgcccgt cgcgcgaaag gccagatcaa tcagggcatg agcggcctcg gtcgcaaagc cgtggcccca atagggttcc cccagccagt aaccgatttc cagcccttcg ccatgtttgt acggatggat gccgcagcac cccatgaaaa tgccggtctc tgcttgcgta atcgcataga tgcaattgcc catctcgcct tttcgcacgc gccccacaaa atcgagggcg ttttctcgcg tatatggatg cggcatgcgg gcaagcatgc tggatacgcg agcattgttg gcaagatagg aaatggcatc cacgtcttcg gcatggggcg ggcgcagaac cagcctttcc gtgacgagaa tgggggcaat ccagcacata gcccagctcc ttttccaagc cgggagatgc atcggcataa tccgcttccc gcttacaaca acttgcttcg tagctttctt cttcgacaac catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccatggta t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVVEEESYE ASCCKREADY ADASPGLEKE LGYVLDCPIL VTERLVLRPP HAEDVDAISY LANNARVSSM LARMPHPYTR ENALDFVGRV RKGEMGNCIY AITQAETGIF MGCCGIHPYK HGEGLEIGYW LGEPYWGHGF ATEAAHALID LAFRATGIGR LHVSCRASNG GSRRVIHKCG FQFSGMGMAD SLAAGNVPVE HYVLDRRTWI GLRSWQS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggttgtcgaa gaagaaagct acgaagcaag ttgttgtaag cgggaagcgg attatgccga tgcatctccc ggcttggaaa aggagctggg ctatgtgctg gattgcccca ttctcgtcac ggaaaggctg gttctgcgcc cgccccatgc cgaagacgtg gatgccattt cctatcttgc caacaatgct cgcgtatcca gcatgcttgc ccgcatgccg catccatata cgcgagaaaa cgccctcgat tttgtggggc gcgtgcgaaa aggcgagatg ggcaattgca tctatgcgat tacgcaagca gagaccggca ttttcatggg gtgctgcggc atccatccgt acaaacatgg cgaagggctg gaaatcggtt actggctggg ggaaccctat tggggccacg gctttgcgac cgaggccgct catgccctga ttgatctggc ctttcgcgcg acgggcatcg ggcggctgca tgtttcatgc cgggccagca atggcggctc gcgccgggtg atccacaagt gcggctttca gttcagcggc atgggcatgg cggattcgct tgccgcagga aatgtgccgg tcgagcacta tgttctggac aggcgtacat ggatcggtct gagaagctgg caatcataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BrabA.17315.a.A1.PW31491
PURIFICATION DATe: 6/10/2011
CONCENTRATION: 40.79mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 100µl
PERCENT IDENTITY: 51
PERCENT COVERAGE: 71
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVVEEESYE ASCCKREADY ADASPGLEKE LGYVLDCPHS RHGKAGSAPA PCRRRGCHFL SCQQCSRIQH ACPHAASIYA RKRPRFCGAR AKRRDGQLHL CDYASRDRHF HGVLRHPSVQ TWRRAGNRLL AGGTLLGPRL CDRGRSCPD
Validated NT Sequence
ccagcttctc agaccgatcc atgtacgcct gtccagaaca tagtgctcga ccggcacatt tcntgcggca agcgaatccg ccatgcccat gccgctgaac tgaaagccgc acttgtggat cacccggcgc gagccgccat tgctggcccg gcatgaaaca tgcagccgcc cgatgcccgt cgcgcgaaag gccagatcaa tcagggcatg agcggcctcg gtcgcaaagc cgtggcccca atagggttcc cccagccagt aaccgatttc cagcccttcg ccatgtttgt acggatggat gccgcagcac cccatgaaaa tgccggtctc tgcttgcgta atcgcataga tgcaattgcc catctcgcct tttcgcacgc gccccacaaa atcgagggcg ttttctcgcg tatatggatg cggcatgcgg gcaagcatgc tggatacgcg agcattgttg gcaagatagg aaatggcatc cacgtcttcg gcatggggcg ggcgcagaac cagcctttcc gtgacgagaa tgggggcaat ccagcacata gcccagctcc ttttccaagc cgggagatgc atcggcataa tccgcttccc gcttacaaca acttgcttcg tagctttctt cttcgacaac catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccatggta t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVVEEESYE ASCCKREADY ADASPGLEKE LGYVLDCPIL VTERLVLRPP HAEDVDAISY LANNARVSSM LARMPHPYTR ENALDFVGRV RKGEMGNCIY AITQAETGIF MGCCGIHPYK HGEGLEIGYW LGEPYWGHGF ATEAAHALID LAFRATGIGR LHVSCRASNG GSRRVIHKCG FQFSGMGMAD SLAAGNVPVE HYVLDRRTWI GLRSWQS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTTGTCGAA GAAGAAAGCT ACGAAGCAAG TTGTTGTAAG CGGGAAGCGG ATTATGCCGA TGCATCTCCC GGCTTGGAAA AGGAGCTGGG CTATGTGCTG GATTGCCCCA TTCTCGTCAC GGAAAGGCTG GTTCTGCGCC CGCCCCATGC CGAAGACGTG GATGCCATTT CCTATCTTGC CAACAATGCT CGCGTATCCA GCATGCTTGC CCGCATGCCG CATCCATATA CGCGAGAAAA CGCCCTCGAT TTTGTGGGGC GCGTGCGAAA AGGCGAGATG GGCAATTGCA TCTATGCGAT TACGCAAGCA GAGACCGGCA TTTTCATGGG GTGCTGCGGC ATCCATCCGT ACAAACATGG CGAAGGGCTG GAAATCGGTT ACTGGCTGGG GGAACCCTAT TGGGGCCACG GCTTTGCGAC CGAGGCCGCT CATGCCCTGA TTGATCTGGC CTTTCGCGCG ACGGGCATCG GGCGGCTGCA TGTTTCATGC CGGGCCAGCA ATGGCGGCTC GCGCCGGGTG ATCCACAAGT GCGGCTTTCA GTTCAGCGGC ATGGGCATGG CGGATTCGCT TGCCGCAGGA AATGTGCCGG TCGAGCACTA TGTTCTGGAC AGGCGTACAT GGATCGGTCT GAGAAGCTGG CAATCATAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA