SSGCID: Target Page

BrabA.17312.a

Hsp33 protein

CENTER ID:	BrabA.17312.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17312.a.A1.GE31584	Full length( BrabA.17312.a )		1	340

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.352
RefSeq:	YP_413801.1
UniProt:	Q2YPH1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSDKTSNEHT EAENIQTGLG GFNFAGDDAV VPFQVEGLDV RGRAVQLGPS INAILKRHEY PEEVARLLAE ATVLTVLLGT SLKFDGKFIF QTQSDGPVDM LVVDFRTPRS VRAYARYDEA RLKEAIAANK TRPEELLGNG TLAFTVDQGI YTQRYQGIVA LDGSTLEEIA VTYFRQSEQI PTDLKLSVAK LIERGADGKP VEQWRAGGLI IQFLPESELR ARVPDLPGGD GDDSQLIHHP EDDAWDEARS LVGTIESSEL TDPQVGSERL LYRLFHERGV RVFDSIAVID ECSCSREKIA GVLSGFTAEE IKDSVEDGKI SVTCEFCSKL YQFDPAEFTK

NT Sequence

TTGTCCGACA AGACCAGTAA TGAGCATACC GAAGCCGAAA ATATCCAGAC CGGTCTGGGC GGCTTCAATT TCGCAGGCGA TGATGCCGTC GTGCCCTTCC AGGTGGAAGG GCTCGATGTG CGTGGCCGCG CGGTTCAGCT TGGCCCTAGC ATCAACGCGA TACTTAAGCG CCATGAATAC CCGGAAGAAG TTGCGCGCCT TCTGGCGGAA GCGACGGTCC TGACCGTTCT GCTCGGCACT TCGCTGAAAT TCGACGGAAA GTTCATCTTC CAGACCCAGT CCGACGGTCC GGTGGATATG CTGGTGGTGG ATTTTCGTAC GCCGCGTTCG GTGCGCGCCT ATGCGCGTTA CGACGAAGCG CGCCTGAAGG AAGCCATTGC CGCAAACAAG ACTCGCCCGG AAGAGTTGCT CGGCAATGGA ACGCTCGCTT TCACCGTGGA TCAGGGGATC TATACCCAGC GTTATCAGGG CATCGTTGCG CTTGATGGTT CCACGCTGGA GGAGATCGCC GTCACCTATT TCCGCCAGTC GGAGCAGATT CCGACCGACC TGAAGCTCAG CGTCGCCAAG CTCATCGAAC GCGGCGCGGA CGGCAAGCCG GTCGAGCAAT GGCGTGCGGG CGGCCTCATC ATCCAGTTCC TGCCGGAATC GGAATTGCGC GCGCGCGTTC CCGATCTTCC CGGCGGTGAC GGCGATGACA GCCAATTGAT TCATCACCCG GAAGACGATG CCTGGGATGA AGCCCGTTCG CTGGTGGGAA CCATCGAAAG CTCGGAACTG ACCGATCCTC AGGTCGGCTC GGAACGGCTT CTTTATCGCC TGTTCCATGA ACGCGGCGTG CGGGTGTTCG ATTCCATTGC GGTTATCGAT GAATGTTCGT GCTCGCGTGA GAAGATTGCA GGCGTGCTGT CAGGATTCAC GGCCGAGGAA ATCAAGGACA GCGTGGAAGA CGGGAAAATT TCCGTCACCT GCGAATTTTG CTCCAAGCTT TATCAGTTCG ATCCGGCTGA ATTCACCAAA

Details for BrabA.17312.a.A1.GE31584

HARVESTED ON:	3/1/2011
SEQUENCED ON:	3/7/2011
EXPECTED MW:	39kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	85
PERCENT COVERAGE:	5

Validated AA Sequence

MLVVDFRTPX SVRXYARXDE A

Validated NT Sequence

gcgcttcgtc ntaacgcgca tagncgcgca ccgaangcgg cgtacgaaaa tccaccacca gcatatccac cggaccgtcg gactgggtct ggaagatgaa ctttccgtcg aatttcagcg aagtgccgag cagaacggtc agnaccgtcg cnt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLSDKTSNE HTEAENIQTG LGGFNFAGDD AVVPFQVEGL DVRGRAVQLG PSINAILKRH EYPEEVARLL AEATVLTVLL GTSLKFDGKF IFQTQSDGPV DMLVVDFRTP RSVRAYARYD EARLKEAIAA NKTRPEELLG NGTLAFTVDQ GIYTQRYQGI VALDGSTLEE IAVTYFRQSE QIPTDLKLSV AKLIERGADG KPVEQWRAGG LIIQFLPESE LRARVPDLPG GDGDDSQLIH HPEDDAWDEA RSLVGTIESS ELTDPQVGSE RLLYRLFHER GVRVFDSIAV IDECSCSREK IAGVLSGFTA EEIKDSVEDG KISVTCEFCS KLYQFDPAEF TK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgtccgac aagaccagta atgagcatac cgaagccgaa aatatccaga ccggtctggg cggcttcaat ttcgcaggcg atgatgccgt cgtgcccttc caggtggaag ggctcgatgt gcgtggccgc gcggttcagc ttggccctag catcaacgcg atacttaagc gccatgaata cccggaagaa gttgcgcgcc ttctggcgga agcgacggtc ctgaccgttc tgctcggcac ttcgctgaaa ttcgacggaa agttcatctt ccagacccag tccgacggtc cggtggatat gctggtggtg gattttcgta cgccgcgttc ggtgcgcgcc tatgcgcgtt acgacgaagc gcgcctgaag gaagccattg ccgcaaacaa gactcgcccg gaagagttgc tcggcaatgg aacgctcgct ttcaccgtgg atcaggggat ctatacccag cgttatcagg gcatcgttgc gcttgatggt tccacgctgg aggagatcgc cgtcacctat ttccgccagt cggagcagat tccgaccgac ctgaagctca gcgtcgccaa gctcatcgaa cgcggcgcgg acggcaagcc ggtcgagcaa tggcgtgcgg gcggcctcat catccagttc ctgccggaat cggaattgcg cgcgcgcgtt cccgatcttc ccggcggtga cggcgatgac agccaattga ttcatcaccc ggaagacgat gcctgggatg aagcccgttc gctggtggga accatcgaaa gctcggaact gaccgatcct caggtcggct cggaacggct tctttatcgc ctgttccatg aacgcggcgt gcgggtgttc gattccattg cggttatcga tgaatgttcg tgctcgcgtg agaagattgc aggcgtgctg tcaggattca cggccgagga aatcaaggac agcgtggaag acgggaaaat ttccgtcacc tgcgaatttt gctccaagct ttatcagttc gatccggctg aattcaccaa ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa