SSGCID: Target Page

BrabA.17302.c

ISBm1, transposase orfA

CENTER ID:	BrabA.17302.c
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.17302.c.A1.GE32614	Full length( BrabA.17302.c )		1	142

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.573
RefSeq:	YP_414000.1
UniProt:	Q2YMP2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTRRRYELTD HEWSIISPLL PNKPRGVARV DDRRVLNGIL WRFRTGSPWA EVPERYGPPT ACYNRFVRWR KAGVWDRLFE TVPKAYDGDI VMIDSTCVRV HQHAATGKKG METMVAWDVP AAGLRAKSTR SLMPKVARSI YV

NT Sequence

ATGACGCGTC GTCGCTACGA ACTAACTGAC CACGAATGGT CGATTATATC GCCGTTGTTG CCCAACAAAC CACGCGGCGT TGCTCGCGTT GATGATCGGC GGGTGCTGAA TGGCATCCTG TGGCGATTTC GAACGGGCTC GCCCTGGGCG GAAGTTCCCG AACGCTATGG CCCGCCGACG GCCTGTTACA ACCGCTTCGT GCGCTGGCGC AAAGCCGGGG TCTGGGATCG GCTTTTTGAA ACGGTGCCCA AGGCCTACGA TGGCGACATC GTCATGATCG ACTCGACCTG TGTCCGCGTT CACCAGCACG CGGCCACAGG AAAAAAGGGG ATGGAGACGA TGGTGGCATG GGACGTTCCC GCGGCGGGCT TACGAGCAAA ATCCACGCGC TCGTTGATGC CGAAGGTCGC CCGGTCAATC TACGTC

Details for BrabA.17302.c.A1.GE32614

HARVESTED ON:	5/23/2011
SEQUENCED ON:	5/31/2011
EXPECTED MW:	18kDa
OBSERVED MW:	18kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQSPG SMTRRRYELT DHEWSIISPL LPNKPRGVAR VDDRRVLNGI LWRFRTGSPW AEVPERYGPP TACYNRFVRW RKAGVWDRLF ETVPKAYDGD IVMIDSTCVR VHQHAATGKK GMETMVAWDV PAAGLRAKST RSLMPKVARS IYV

Validated NT Sequence

gnnnnnnnnn nnnnnnagga gnnataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccaaa gtcctggttc gatgacgcgt cgtcgctacg aactaactga ccacgaatgg tcgattatat cgccgttgtt gcccaacaaa ccacgcggcg ttgctcgcgt tgatgatcgg cgggtgctga atggcatcct gtggcgattt cgaacgggct cgccctgggc ggaagttccc gaacgctatg gcccgccgac ggcctgttac aaccgcttcg tgcgctggcg caaagccggg gtctgggatc ggctttttga aacggtgccc aaggcctacg atggcgacat cgtcatgatc gactcgacct gtgtccgcgt tcaccagcac gcggccacag gaaaaaaggg gatggagacg atggtggcat gggacgttcc cgcggcgggc ttacgagcaa aatccacgcg ctcgttgatg ccgaaggtcg cccggtcaat ctacgtctaa taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccanc gcna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTRRRYELT DHEWSIISPL LPNKPRGVAR VDDRRVLNGI LWRFRTGSPW AEVPERYGPP TACYNRFVRW RKAGVWDRLF ETVPKAYDGD IVMIDSTCVR VHQHAATGKK GMETMVAWDV PAAGLRAKST RSLMPKVARS IYV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgcgtcgt cgctacgaac taactgacca cgaatggtcg attatatcgc cgttgttgcc caacaaacca cgcggcgttg ctcgcgttga tgatcggcgg gtgctgaatg gcatcctgtg gcgatttcga acgggctcgc cctgggcgga agttcccgaa cgctatggcc cgccgacggc ctgttacaac cgcttcgtgc gctggcgcaa agccggggtc tgggatcggc tttttgaaac ggtgcccaag gcctacgatg gcgacatcgt catgatcgac tcgacctgtg tccgcgttca ccagcacgcg gccacaggaa aaaaggggat ggagacgatg gtggcatggg acgttcccgc ggcgggctta cgagcaaaat ccacgcgctc gttgatgccg aaggtcgccc ggtcaatcta cgtctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa