BrabA.17299.a

Protein tolB

CENTER ID: BrabA.17299.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17299.a.A2.GE31576 Mature protein( BrabA.17299.a ) 33 443
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17299.a.A2.PS01070 Mature protein( BrabA.17299.a ) 33 443

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.1772
RefSeq: YP_415059.1
UniProt: Q2YRD7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKIGIINTKI RTVFSAFACM IAASLVCTMP ARAVVEININ KGVIEPLPIA ITDFLSADQL GSNITSVIAA DLERSGLFAP IDKGAFIEKI SNPDAAPRFE DWKVINAQAL VTGRITKQPD GRLKAEFRLW DTFGGQQMIG QQFFTTPDNW RRVAHIIADA IYERLTGDKG YFDTRVVFVD ESGPAQKRVK RLAIMDQDGA NVRFISDGRA LSLTPRFSPN RQEVTYMSFE GGSPKVYLLQ LETGQRELVG NFPGMTIAPR FSPDGQKVVM SLLQDDGSAN IYTMDLRNRT TTRLTSSQAI DTGASYSPDG SQIVFTSDRG GRPQLYVMGA DGSNPRRISM GDGSYSTPVW SPRGDLIAFT KQSQGQFSIG VMKTDGSGER LLTSGFHNEG PTWAPNGRVL MFFRKAAGAG GPKLFTIDLT GRNERQIQTP NFASDPAWSP LLE
NT Sequence
ATGAAAATCG GCATCATCAA TACAAAGATC CGGACGGTCT TTTCGGCTTT CGCGTGTATG ATTGCCGCAA GCCTCGTCTG CACCATGCCG GCGCGCGCTG TCGTGGAAAT CAACATCAAC AAGGGTGTGA TCGAGCCGCT GCCGATCGCC ATCACCGATT TTCTTTCGGC TGATCAGCTT GGATCGAATA TCACGTCGGT CATTGCGGCC GATCTTGAGC GGTCCGGCCT CTTCGCCCCC ATAGACAAGG GCGCTTTCAT CGAAAAGATC TCCAATCCGG ATGCGGCGCC GCGTTTCGAG GACTGGAAGG TCATCAATGC GCAGGCGCTC GTTACGGGCC GCATCACCAA ACAGCCCGAT GGCAGGCTCA AGGCTGAGTT CCGTCTCTGG GATACATTTG GCGGCCAGCA GATGATCGGC CAGCAATTTT TCACCACGCC GGATAACTGG CGTCGTGTTG CCCATATCAT CGCGGACGCC ATCTATGAGC GCCTGACCGG TGACAAAGGC TATTTCGATA CCCGTGTCGT TTTCGTGGAT GAATCAGGCC CGGCGCAGAA GCGTGTGAAG CGCCTTGCCA TCATGGACCA GGACGGCGCC AATGTGCGTT TTATTTCTGA TGGCCGCGCC CTTTCGCTGA CGCCGCGCTT CTCGCCGAAC CGGCAGGAAG TGACCTATAT GTCGTTTGAG GGCGGCTCGC CGAAGGTCTA TCTGCTGCAA CTTGAAACCG GCCAGCGCGA GCTTGTCGGC AACTTCCCCG GCATGACTAT TGCGCCGCGT TTTTCGCCCG ACGGCCAGAA GGTCGTGATG AGCCTGCTGC AGGATGACGG CAGCGCCAAT ATCTATACGA TGGATCTGCG CAATCGCACG ACCACGCGCC TGACCAGCAG CCAGGCGATC GATACGGGTG CTTCCTATTC GCCGGATGGT TCGCAGATTG TCTTCACATC CGACCGTGGC GGGCGCCCGC AGCTCTATGT CATGGGTGCC GATGGCTCCA ATCCGCGCCG TATCTCGATG GGTGACGGCA GCTATTCCAC GCCGGTCTGG TCCCCGCGCG GTGATCTCAT CGCTTTCACC AAGCAATCGC AGGGCCAGTT CTCCATTGGT GTGATGAAGA CCGATGGTTC GGGTGAGCGT CTGCTGACAT CGGGCTTTCA CAATGAAGGC CCGACATGGG CCCCGAATGG CCGCGTGCTG ATGTTTTTCC GCAAGGCAGC CGGTGCAGGC GGCCCGAAAC TCTTCACCAT CGACCTGACC GGCCGTAACG AACGCCAGAT CCAGACGCCG AATTTTGCTT CCGACCCGGC CTGGTCGCCG CTGCTCGAA
Details for BrabA.17299.a.A2.GE31576
HARVESTED ON: 3/1/2011
SEQUENCED ON: 3/7/2011
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 57
Validated AA Sequence
MDQDGANVRF ISDGRALSLT PRFSPNXQEV TYMSFEGGSP KVYLLQLETG QRELVGNFPG MTIAPRFSPD GQKVVMSLLQ DDGSANIYTM DLRNRTTTRL TSSQAIDTGA SYSPDGSQIV FTSDRGGRPQ LYVMGADGSN PRRISMGDGS YSTPVWSPRG DLIAFTKQSQ GQFSIGVMKT DGSGERLLTS GFHNEGPTWA PNGRVLMFFR KAAGAGGPKL FTIDLTGRNE RQIQTPNFAS DPAWSPLL
Validated NT Sequence
ttcgagcagc ggcgaccagg ccgggtcgga agcaaaattc ggcgtctgga tctggcgttc gttacggccg gtcaggtcga tggtgaagag tttcgggccg cctgcaccgg ctgccttgcg gaaaaacatc agcacgcggc cattcggggc ccatgtcggg ccttcattgt gaaagcccga tgtcagcaga cgctcacccg aaccatcggt cttcatcaca ccaatggaga actggccctg cgattgcttg gtgaaagcga tgagatcacc gcgcggggac cagaccggcg tggaatagct gccgtcaccc atcgagatac ggcgcggatt ggagccatcg gcacccatga catagagctg cgggcgcccg ccacggtcgg atgtgaagac aatctgcgaa ccatccggcg aataggaagc acccgtatcg atcgcctggc tgctggtcag gcgcgtggtc gtgcgattgc gcagatccat cgtatagata ttggcgctgc cgtcatcctg cagcaggctc atcacgacct tctggccgtc gggcgaaaaa cgcggcgcaa tagtcatgcc ggggaagttg ccgacnagct cgcgctggcc ggtttcaagt tgcagcagat agaccttcgg cgagccgccc tcaaacgaca tataggtcac ttcctgccng ttcggcgaga agcgcggcgt cagcgaaagg gcgcggccat cagaaataaa acgcacattg gcgccgtcct ggtccatgat ggcaaggcgc ttcacacgct tctgcgccgg gcctgnttca tccacgaaaa cgacacgggt atcgaaatan cctttgtcac cngtcnnnng ctcatagatg gcgtccgcga tganntgggc ancncgacnc c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAVVEININ KGVIEPLPIA ITDFLSADQL GSNITSVIAA DLERSGLFAP IDKGAFIEKI SNPDAAPRFE DWKVINAQAL VTGRITKQPD GRLKAEFRLW DTFGGQQMIG QQFFTTPDNW RRVAHIIADA IYERLTGDKG YFDTRVVFVD ESGPAQKRVK RLAIMDQDGA NVRFISDGRA LSLTPRFSPN RQEVTYMSFE GGSPKVYLLQ LETGQRELVG NFPGMTIAPR FSPDGQKVVM SLLQDDGSAN IYTMDLRNRT TTRLTSSQAI DTGASYSPDG SQIVFTSDRG GRPQLYVMGA DGSNPRRISM GDGSYSTPVW SPRGDLIAFT KQSQGQFSIG VMKTDGSGER LLTSGFHNEG PTWAPNGRVL MFFRKAAGAG GPKLFTIDLT GRNERQIQTP NFASDPAWSP LLE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgtcgtg gaaatcaaca tcaacaaggg tgtgatcgag ccgctgccga tcgccatcac cgattttctt tcggctgatc agcttggatc gaatatcacg tcggtcattg cggccgatct tgagcggtcc ggcctcttcg cccccataga caagggcgct ttcatcgaaa agatctccaa tccggatgcg gcgccgcgtt tcgaggactg gaaggtcatc aatgcgcagg cgctcgttac gggccgcatc accaaacagc ccgatggcag gctcaaggct gagttccgtc tctgggatac atttggcggc cagcagatga tcggccagca atttttcacc acgccggata actggcgtcg tgttgcccat atcatcgcgg acgccatcta tgagcgcctg accggtgaca aaggctattt cgatacccgt gtcgttttcg tggatgaatc aggcccggcg cagaagcgtg tgaagcgcct tgccatcatg gaccaggacg gcgccaatgt gcgttttatt tctgatggcc gcgccctttc gctgacgccg cgcttctcgc cgaaccggca ggaagtgacc tatatgtcgt ttgagggcgg ctcgccgaag gtctatctgc tgcaacttga aaccggccag cgcgagcttg tcggcaactt ccccggcatg actattgcgc cgcgtttttc gcccgacggc cagaaggtcg tgatgagcct gctgcaggat gacggcagcg ccaatatcta tacgatggat ctgcgcaatc gcacgaccac gcgcctgacc agcagccagg cgatcgatac gggtgcttcc tattcgccgg atggttcgca gattgtcttc acatccgacc gtggcgggcg cccgcagctc tatgtcatgg gtgccgatgg ctccaatccg cgccgtatct cgatgggtga cggcagctat tccacgccgg tctggtcccc gcgcggtgat ctcatcgctt tcaccaagca atcgcagggc cagttctcca ttggtgtgat gaagaccgat ggttcgggtg agcgtctgct gacatcgggc tttcacaatg aaggcccgac atgggccccg aatggccgcg tgctgatgtt tttccgcaag gcagccggtg caggcggccc gaaactcttc accatcgacc tgaccggccg taacgaacgc cagatccaga cgccgaattt tgcttccgac ccggcctggt cgccgctgct cgaataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BrabA.17299.a.A2.PS01070
PURIFICATION DATe: 5/6/2011
CONCENTRATION: 48.07mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 57
Protocol Notes
notes unavailable
Validated AA Sequence
MDQDGANVRF ISDGRALSLT PRFSPNXQEV TYMSFEGGSP KVYLLQLETG QRELVGNFPG MTIAPRFSPD GQKVVMSLLQ DDGSANIYTM DLRNRTTTRL TSSQAIDTGA SYSPDGSQIV FTSDRGGRPQ LYVMGADGSN PRRISMGDGS YSTPVWSPRG DLIAFTKQSQ GQFSIGVMKT DGSGERLLTS GFHNEGPTWA PNGRVLMFFR KAAGAGGPKL FTIDLTGRNE RQIQTPNFAS DPAWSPLL
Validated NT Sequence
ttcgagcagc ggcgaccagg ccgggtcgga agcaaaattc ggcgtctgga tctggcgttc gttacggccg gtcaggtcga tggtgaagag tttcgggccg cctgcaccgg ctgccttgcg gaaaaacatc agcacgcggc cattcggggc ccatgtcggg ccttcattgt gaaagcccga tgtcagcaga cgctcacccg aaccatcggt cttcatcaca ccaatggaga actggccctg cgattgcttg gtgaaagcga tgagatcacc gcgcggggac cagaccggcg tggaatagct gccgtcaccc atcgagatac ggcgcggatt ggagccatcg gcacccatga catagagctg cgggcgcccg ccacggtcgg atgtgaagac aatctgcgaa ccatccggcg aataggaagc acccgtatcg atcgcctggc tgctggtcag gcgcgtggtc gtgcgattgc gcagatccat cgtatagata ttggcgctgc cgtcatcctg cagcaggctc atcacgacct tctggccgtc gggcgaaaaa cgcggcgcaa tagtcatgcc ggggaagttg ccgacnagct cgcgctggcc ggtttcaagt tgcagcagat agaccttcgg cgagccgccc tcaaacgaca tataggtcac ttcctgccng ttcggcgaga agcgcggcgt cagcgaaagg gcgcggccat cagaaataaa acgcacattg gcgccgtcct ggtccatgat ggcaaggcgc ttcacacgct tctgcgccgg gcctgnttca tccacgaaaa cgacacgggt atcgaaatan cctttgtcac cngtcnnnng ctcatagatg gcgtccgcga tganntgggc ancncgacnc c
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAVVEININ KGVIEPLPIA ITDFLSADQL GSNITSVIAA DLERSGLFAP IDKGAFIEKI SNPDAAPRFE DWKVINAQAL VTGRITKQPD GRLKAEFRLW DTFGGQQMIG QQFFTTPDNW RRVAHIIADA IYERLTGDKG YFDTRVVFVD ESGPAQKRVK RLAIMDQDGA NVRFISDGRA LSLTPRFSPN RQEVTYMSFE GGSPKVYLLQ LETGQRELVG NFPGMTIAPR FSPDGQKVVM SLLQDDGSAN IYTMDLRNRT TTRLTSSQAI DTGASYSPDG SQIVFTSDRG GRPQLYVMGA DGSNPRRISM GDGSYSTPVW SPRGDLIAFT KQSQGQFSIG VMKTDGSGER LLTSGFHNEG PTWAPNGRVL MFFRKAAGAG GPKLFTIDLT GRNERQIQTP NFASDPAWSP LLE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTGTCGTG GAAATCAACA TCAACAAGGG TGTGATCGAG CCGCTGCCGA TCGCCATCAC CGATTTTCTT TCGGCTGATC AGCTTGGATC GAATATCACG TCGGTCATTG CGGCCGATCT TGAGCGGTCC GGCCTCTTCG CCCCCATAGA CAAGGGCGCT TTCATCGAAA AGATCTCCAA TCCGGATGCG GCGCCGCGTT TCGAGGACTG GAAGGTCATC AATGCGCAGG CGCTCGTTAC GGGCCGCATC ACCAAACAGC CCGATGGCAG GCTCAAGGCT GAGTTCCGTC TCTGGGATAC ATTTGGCGGC CAGCAGATGA TCGGCCAGCA ATTTTTCACC ACGCCGGATA ACTGGCGTCG TGTTGCCCAT ATCATCGCGG ACGCCATCTA TGAGCGCCTG ACCGGTGACA AAGGCTATTT CGATACCCGT GTCGTTTTCG TGGATGAATC AGGCCCGGCG CAGAAGCGTG TGAAGCGCCT TGCCATCATG GACCAGGACG GCGCCAATGT GCGTTTTATT TCTGATGGCC GCGCCCTTTC GCTGACGCCG CGCTTCTCGC CGAACCGGCA GGAAGTGACC TATATGTCGT TTGAGGGCGG CTCGCCGAAG GTCTATCTGC TGCAACTTGA AACCGGCCAG CGCGAGCTTG TCGGCAACTT CCCCGGCATG ACTATTGCGC CGCGTTTTTC GCCCGACGGC CAGAAGGTCG TGATGAGCCT GCTGCAGGAT GACGGCAGCG CCAATATCTA TACGATGGAT CTGCGCAATC GCACGACCAC GCGCCTGACC AGCAGCCAGG CGATCGATAC GGGTGCTTCC TATTCGCCGG ATGGTTCGCA GATTGTCTTC ACATCCGACC GTGGCGGGCG CCCGCAGCTC TATGTCATGG GTGCCGATGG CTCCAATCCG CGCCGTATCT CGATGGGTGA CGGCAGCTAT TCCACGCCGG TCTGGTCCCC GCGCGGTGAT CTCATCGCTT TCACCAAGCA ATCGCAGGGC CAGTTCTCCA TTGGTGTGAT GAAGACCGAT GGTTCGGGTG AGCGTCTGCT GACATCGGGC TTTCACAATG AAGGCCCGAC ATGGGCCCCG AATGGCCGCG TGCTGATGTT TTTCCGCAAG GCAGCCGGTG CAGGCGGCCC GAAACTCTTC ACCATCGACC TGACCGGCCG TAACGAACGC CAGATCCAGA CGCCGAATTT TGCTTCCGAC CCGGCCTGGT CGCCGCTGCT CGAATAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA